The protective effect of betanin and copper on heart and lung in end‑organ ischemia reperfusion injury
Betanin and copper sulphate have been previously indicated as beneficial agents for ischemia/reperfusion (I/R) as antioxidant compounds in various models. We investigated whether betanin and copper have any protective effects on the heart and lung against I/R injury in rats. Spraque-Dawley rats were...
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| Veröffentlicht in: | Bratislava Medical Journal 2020, Vol.121 (3), p.211-217 |
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| container_title | Bratislava Medical Journal |
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| creator | Tural, K Ozden, O Bilgi, Z Kubat, E Ermutlu, C S Merhan, O Findik Guvendi, K Kucuker, S A |
| description | Betanin and copper sulphate have been previously indicated as beneficial agents for ischemia/reperfusion (I/R) as antioxidant compounds in various models. We investigated whether betanin and copper have any protective effects on the heart and lung against I/R injury in rats.
Spraque-Dawley rats were assigned in groups: Sham (laparotomy only), control (I/R only), betanin treatment (100 mg/kg of betanin administered intraperitoneally (i.p.) 60 minutes before I/R) and copper sulfate treatment group (0.1 mg/kg/day copper sulfate i.p. for 7 days before I/R). Ischemia was induced by clamping the aorta between the left renal artery and aortic bifurcation for 45 minutes. After 48-hour reperfusion, the rats were sacrificed and heart/lung tissues were harvested. Malondialdehyde (MDA), myeloperoxidase (MPO), interleukin 6 (IL-6) levels were determined. Apoptosis was determined via TUNEL assay.
MDA, MPO, IL-6 levels and apoptotic cells were significantly increased in the I/R group. In both treatment groups, MDA and MPO levels were decreased. IL-6 was significantly decreased in response to betanin administration in the heart, but not in the lung; copper had no effect in either area. The numbers of apoptotic cells were significantly decreased in both treatment groups.
Betanin and copper may have protective effects on I/R injury in the heart and lung in rats (Fig. 6, Ref. 39). |
| doi_str_mv | 10.4149/BLL_2020_032 |
| format | Article |
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Spraque-Dawley rats were assigned in groups: Sham (laparotomy only), control (I/R only), betanin treatment (100 mg/kg of betanin administered intraperitoneally (i.p.) 60 minutes before I/R) and copper sulfate treatment group (0.1 mg/kg/day copper sulfate i.p. for 7 days before I/R). Ischemia was induced by clamping the aorta between the left renal artery and aortic bifurcation for 45 minutes. After 48-hour reperfusion, the rats were sacrificed and heart/lung tissues were harvested. Malondialdehyde (MDA), myeloperoxidase (MPO), interleukin 6 (IL-6) levels were determined. Apoptosis was determined via TUNEL assay.
MDA, MPO, IL-6 levels and apoptotic cells were significantly increased in the I/R group. In both treatment groups, MDA and MPO levels were decreased. IL-6 was significantly decreased in response to betanin administration in the heart, but not in the lung; copper had no effect in either area. The numbers of apoptotic cells were significantly decreased in both treatment groups.
Betanin and copper may have protective effects on I/R injury in the heart and lung in rats (Fig. 6, Ref. 39).</description><identifier>ISSN: 0006-9248</identifier><identifier>ISSN: 1336-0345</identifier><identifier>EISSN: 1336-0345</identifier><identifier>DOI: 10.4149/BLL_2020_032</identifier><identifier>PMID: 32115979</identifier><language>eng</language><publisher>Slovakia</publisher><subject>Animals ; Betacyanins - pharmacology ; Copper - pharmacology ; Heart - drug effects ; Lung - drug effects ; Malondialdehyde ; Rats ; Rats, Sprague-Dawley ; Reperfusion Injury - prevention & control</subject><ispartof>Bratislava Medical Journal, 2020, Vol.121 (3), p.211-217</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c395t-bfc7b3057cde0d2f58ac2ff940b3a3f69e44a1d4554937b602772a0d05e3456e3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,4010,27904,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32115979$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tural, K</creatorcontrib><creatorcontrib>Ozden, O</creatorcontrib><creatorcontrib>Bilgi, Z</creatorcontrib><creatorcontrib>Kubat, E</creatorcontrib><creatorcontrib>Ermutlu, C S</creatorcontrib><creatorcontrib>Merhan, O</creatorcontrib><creatorcontrib>Findik Guvendi, K</creatorcontrib><creatorcontrib>Kucuker, S A</creatorcontrib><title>The protective effect of betanin and copper on heart and lung in end‑organ ischemia reperfusion injury</title><title>Bratislava Medical Journal</title><addtitle>Bratisl Lek Listy</addtitle><description>Betanin and copper sulphate have been previously indicated as beneficial agents for ischemia/reperfusion (I/R) as antioxidant compounds in various models. We investigated whether betanin and copper have any protective effects on the heart and lung against I/R injury in rats.
Spraque-Dawley rats were assigned in groups: Sham (laparotomy only), control (I/R only), betanin treatment (100 mg/kg of betanin administered intraperitoneally (i.p.) 60 minutes before I/R) and copper sulfate treatment group (0.1 mg/kg/day copper sulfate i.p. for 7 days before I/R). Ischemia was induced by clamping the aorta between the left renal artery and aortic bifurcation for 45 minutes. After 48-hour reperfusion, the rats were sacrificed and heart/lung tissues were harvested. Malondialdehyde (MDA), myeloperoxidase (MPO), interleukin 6 (IL-6) levels were determined. Apoptosis was determined via TUNEL assay.
MDA, MPO, IL-6 levels and apoptotic cells were significantly increased in the I/R group. In both treatment groups, MDA and MPO levels were decreased. IL-6 was significantly decreased in response to betanin administration in the heart, but not in the lung; copper had no effect in either area. The numbers of apoptotic cells were significantly decreased in both treatment groups.
Betanin and copper may have protective effects on I/R injury in the heart and lung in rats (Fig. 6, Ref. 39).</description><subject>Animals</subject><subject>Betacyanins - pharmacology</subject><subject>Copper - pharmacology</subject><subject>Heart - drug effects</subject><subject>Lung - drug effects</subject><subject>Malondialdehyde</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Reperfusion Injury - prevention & control</subject><issn>0006-9248</issn><issn>1336-0345</issn><issn>1336-0345</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkMtOwzAQRS0EoqWwY428ZEFg4kdSL6HiJVViU9aWk4zbVIlT7ASpO36BX-RLMFAQqxnNnLmaewk5TeFSpEJd3cznmgEDDZztkXHKeZYAF3KfjAEgSxQT0xE5CmENILhMs0My4ixNpcrVmKwWK6Qb3_VY9vUrUrQ2drSztMDeuNpR4ypadpsNeto5ukLj--9ZM7gljXt01cfbe-eXxtE6lCtsa0M9Rt4OoY4ntVsPfntMDqxpAp7s6oQ8390uZg_J_On-cXY9T0quZJ8UtswLDjIvK4SKWTk1JbNWCSi44TZTKIRJKyGlUDwvMmB5zgxUIDF6zpBPyPmPbjT1MmDodRu_wqYxDrshaMYzNc2nXLCIXvygpe9C8Gj1xtet8Vudgv7KVv_PNuJnO-WhaLH6g3_D5J9inHYr</recordid><startdate>2020</startdate><enddate>2020</enddate><creator>Tural, K</creator><creator>Ozden, O</creator><creator>Bilgi, Z</creator><creator>Kubat, E</creator><creator>Ermutlu, C S</creator><creator>Merhan, O</creator><creator>Findik Guvendi, K</creator><creator>Kucuker, S A</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>2020</creationdate><title>The protective effect of betanin and copper on heart and lung in end‑organ ischemia reperfusion injury</title><author>Tural, K ; Ozden, O ; Bilgi, Z ; Kubat, E ; Ermutlu, C S ; Merhan, O ; Findik Guvendi, K ; Kucuker, S A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c395t-bfc7b3057cde0d2f58ac2ff940b3a3f69e44a1d4554937b602772a0d05e3456e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Animals</topic><topic>Betacyanins - pharmacology</topic><topic>Copper - pharmacology</topic><topic>Heart - drug effects</topic><topic>Lung - drug effects</topic><topic>Malondialdehyde</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Reperfusion Injury - prevention & control</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tural, K</creatorcontrib><creatorcontrib>Ozden, O</creatorcontrib><creatorcontrib>Bilgi, Z</creatorcontrib><creatorcontrib>Kubat, E</creatorcontrib><creatorcontrib>Ermutlu, C S</creatorcontrib><creatorcontrib>Merhan, O</creatorcontrib><creatorcontrib>Findik Guvendi, K</creatorcontrib><creatorcontrib>Kucuker, S A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Bratislava Medical Journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tural, K</au><au>Ozden, O</au><au>Bilgi, Z</au><au>Kubat, E</au><au>Ermutlu, C S</au><au>Merhan, O</au><au>Findik Guvendi, K</au><au>Kucuker, S A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The protective effect of betanin and copper on heart and lung in end‑organ ischemia reperfusion injury</atitle><jtitle>Bratislava Medical Journal</jtitle><addtitle>Bratisl Lek Listy</addtitle><date>2020</date><risdate>2020</risdate><volume>121</volume><issue>3</issue><spage>211</spage><epage>217</epage><pages>211-217</pages><issn>0006-9248</issn><issn>1336-0345</issn><eissn>1336-0345</eissn><abstract>Betanin and copper sulphate have been previously indicated as beneficial agents for ischemia/reperfusion (I/R) as antioxidant compounds in various models. We investigated whether betanin and copper have any protective effects on the heart and lung against I/R injury in rats.
Spraque-Dawley rats were assigned in groups: Sham (laparotomy only), control (I/R only), betanin treatment (100 mg/kg of betanin administered intraperitoneally (i.p.) 60 minutes before I/R) and copper sulfate treatment group (0.1 mg/kg/day copper sulfate i.p. for 7 days before I/R). Ischemia was induced by clamping the aorta between the left renal artery and aortic bifurcation for 45 minutes. After 48-hour reperfusion, the rats were sacrificed and heart/lung tissues were harvested. Malondialdehyde (MDA), myeloperoxidase (MPO), interleukin 6 (IL-6) levels were determined. Apoptosis was determined via TUNEL assay.
MDA, MPO, IL-6 levels and apoptotic cells were significantly increased in the I/R group. In both treatment groups, MDA and MPO levels were decreased. IL-6 was significantly decreased in response to betanin administration in the heart, but not in the lung; copper had no effect in either area. The numbers of apoptotic cells were significantly decreased in both treatment groups.
Betanin and copper may have protective effects on I/R injury in the heart and lung in rats (Fig. 6, Ref. 39).</abstract><cop>Slovakia</cop><pmid>32115979</pmid><doi>10.4149/BLL_2020_032</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Betacyanins - pharmacology Copper - pharmacology Heart - drug effects Lung - drug effects Malondialdehyde Rats Rats, Sprague-Dawley Reperfusion Injury - prevention & control |
| title | The protective effect of betanin and copper on heart and lung in end‑organ ischemia reperfusion injury |
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