Quantification of Proline-containing Cyclic Dipeptides by LC-MS/MS
The cyclic dipeptides, 2,5-diketopiperazines (DKPs), draw attention as bioactive and taste compounds. DKPs, especially those containing Proline (Pro), are contained in heated and fermented foods. Herein, we developed a method for a simultaneous quantitative analysis of Pro-containing DKPs using LC-M...
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| Veröffentlicht in: | Analytical Sciences 2020/08/10, Vol.36(8), pp.977-980 |
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| container_title | Analytical Sciences |
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| creator | OTSUKA, Yuuki SHIMAMURA, Tomoko SAKAJI, Michio ARITA, Hikaru KASHIWAGI, Takehiro UKEDA, Hiroyuki |
| description | The cyclic dipeptides, 2,5-diketopiperazines (DKPs), draw attention as bioactive and taste compounds. DKPs, especially those containing Proline (Pro), are contained in heated and fermented foods. Herein, we developed a method for a simultaneous quantitative analysis of Pro-containing DKPs using LC-MS/MS. After optimizing the LC-MS/MS conditions, the developed method was applied to quantify Pro-containing DKPs in Goishi tea, which is a post-fermented tea produced by a two-step aerobic-anaerobic fermentation process. Consequently, 17 kinds of Pro-containing DKPs could be quantified, and the total amount of Pro-containing DKPs was 3.40 mg/L. The recovery in a spiked test was 93 – 117%, which is satisfactory. We believe that the developed method can be used to elucidate the role of Pro-containing DKPs in food. |
| doi_str_mv | 10.2116/analsci.19P470 |
| format | Article |
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DKPs, especially those containing Proline (Pro), are contained in heated and fermented foods. Herein, we developed a method for a simultaneous quantitative analysis of Pro-containing DKPs using LC-MS/MS. After optimizing the LC-MS/MS conditions, the developed method was applied to quantify Pro-containing DKPs in Goishi tea, which is a post-fermented tea produced by a two-step aerobic-anaerobic fermentation process. Consequently, 17 kinds of Pro-containing DKPs could be quantified, and the total amount of Pro-containing DKPs was 3.40 mg/L. The recovery in a spiked test was 93 – 117%, which is satisfactory. We believe that the developed method can be used to elucidate the role of Pro-containing DKPs in food.</description><identifier>ISSN: 0910-6340</identifier><identifier>EISSN: 1348-2246</identifier><identifier>DOI: 10.2116/analsci.19P470</identifier><identifier>PMID: 32115462</identifier><language>eng</language><publisher>Singapore: The Japan Society for Analytical Chemistry</publisher><subject>Anaerobic processes ; Analytical Chemistry ; Chemistry ; Chromatography, Liquid - methods ; Cyclic dipeptide ; Cyclic dipeptides ; Fermentation ; Fermented food ; LC-MS/MS ; Limit of Detection ; Peptides, Cyclic - analysis ; Peptides, Cyclic - chemistry ; post-fermented tea ; Pro-containing DKPs ; Proline ; Proline - chemistry ; Tandem Mass Spectrometry - methods ; Tea</subject><ispartof>Analytical Sciences, 2020/08/10, Vol.36(8), pp.977-980</ispartof><rights>2020 by The Japan Society for Analytical Chemistry</rights><rights>The Japan Society for Analytical Chemistry 2020</rights><rights>Copyright Japan Science and Technology Agency 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c658t-f0dc5e21aaa404ce641d776b43114aa384e1226df79a56b6eaaf355e699d4cc23</citedby><cites>FETCH-LOGICAL-c658t-f0dc5e21aaa404ce641d776b43114aa384e1226df79a56b6eaaf355e699d4cc23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.2116/analsci.19P470$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.2116/analsci.19P470$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,1877,27903,27904,41467,42536,51297</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32115462$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>OTSUKA, Yuuki</creatorcontrib><creatorcontrib>SHIMAMURA, Tomoko</creatorcontrib><creatorcontrib>SAKAJI, Michio</creatorcontrib><creatorcontrib>ARITA, Hikaru</creatorcontrib><creatorcontrib>KASHIWAGI, Takehiro</creatorcontrib><creatorcontrib>UKEDA, Hiroyuki</creatorcontrib><title>Quantification of Proline-containing Cyclic Dipeptides by LC-MS/MS</title><title>Analytical Sciences</title><addtitle>ANAL. SCI</addtitle><addtitle>Anal Sci</addtitle><description>The cyclic dipeptides, 2,5-diketopiperazines (DKPs), draw attention as bioactive and taste compounds. DKPs, especially those containing Proline (Pro), are contained in heated and fermented foods. Herein, we developed a method for a simultaneous quantitative analysis of Pro-containing DKPs using LC-MS/MS. After optimizing the LC-MS/MS conditions, the developed method was applied to quantify Pro-containing DKPs in Goishi tea, which is a post-fermented tea produced by a two-step aerobic-anaerobic fermentation process. Consequently, 17 kinds of Pro-containing DKPs could be quantified, and the total amount of Pro-containing DKPs was 3.40 mg/L. The recovery in a spiked test was 93 – 117%, which is satisfactory. We believe that the developed method can be used to elucidate the role of Pro-containing DKPs in food.</description><subject>Anaerobic processes</subject><subject>Analytical Chemistry</subject><subject>Chemistry</subject><subject>Chromatography, Liquid - methods</subject><subject>Cyclic dipeptide</subject><subject>Cyclic dipeptides</subject><subject>Fermentation</subject><subject>Fermented food</subject><subject>LC-MS/MS</subject><subject>Limit of Detection</subject><subject>Peptides, Cyclic - analysis</subject><subject>Peptides, Cyclic - chemistry</subject><subject>post-fermented tea</subject><subject>Pro-containing DKPs</subject><subject>Proline</subject><subject>Proline - chemistry</subject><subject>Tandem Mass Spectrometry - methods</subject><subject>Tea</subject><issn>0910-6340</issn><issn>1348-2246</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kDtPwzAURi0EgvJYGVEkFpa0fsVJRmh5SUWAgNm6dW6Kq9QpdjL035MqpUhILNfDPd93rUPIOaNDzpgagYMqGDtk-YtM6R4ZMCGzmHOp9smA5ozGSkh6RI5DWFDKeMb5ITkSXTaRig_IzWsLrrGlNdDY2kV1Gb34urIOY1O7Bqyzbh6N16ayJprYFa4aW2CIZutoOo6f3kZPb6fkoOw-gWfb94R83N2-jx_i6fP94_h6GhuVZE1c0sIkyBkASCoNKsmKNFUzKRiTACKTyDhXRZnmkKiZQoBSJAmqPC-kMVyckKu-d-XrrxZDo5c2GKwqcFi3QXOh8ixNhUw79PIPuqhbv1GluRS5yHLFVUcNe8r4OgSPpV55uwS_1ozqjV29tat7u13gYlvbzpZY7PAfnR0w6oHQrdwc_e_dfysnfWIRGpjjrhJ8Y02FO1wonW1GH9utzSd4jU58Awl8nhM</recordid><startdate>20200810</startdate><enddate>20200810</enddate><creator>OTSUKA, Yuuki</creator><creator>SHIMAMURA, Tomoko</creator><creator>SAKAJI, Michio</creator><creator>ARITA, Hikaru</creator><creator>KASHIWAGI, Takehiro</creator><creator>UKEDA, Hiroyuki</creator><general>The Japan Society for Analytical Chemistry</general><general>Springer Nature Singapore</general><general>Japan Science and Technology Agency</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SE</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>FR3</scope><scope>H8G</scope><scope>JG9</scope><scope>L7M</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20200810</creationdate><title>Quantification of Proline-containing Cyclic Dipeptides by LC-MS/MS</title><author>OTSUKA, Yuuki ; SHIMAMURA, Tomoko ; SAKAJI, Michio ; ARITA, Hikaru ; KASHIWAGI, Takehiro ; UKEDA, Hiroyuki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c658t-f0dc5e21aaa404ce641d776b43114aa384e1226df79a56b6eaaf355e699d4cc23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Anaerobic processes</topic><topic>Analytical Chemistry</topic><topic>Chemistry</topic><topic>Chromatography, Liquid - methods</topic><topic>Cyclic dipeptide</topic><topic>Cyclic dipeptides</topic><topic>Fermentation</topic><topic>Fermented food</topic><topic>LC-MS/MS</topic><topic>Limit of Detection</topic><topic>Peptides, Cyclic - analysis</topic><topic>Peptides, Cyclic - chemistry</topic><topic>post-fermented tea</topic><topic>Pro-containing DKPs</topic><topic>Proline</topic><topic>Proline - chemistry</topic><topic>Tandem Mass Spectrometry - methods</topic><topic>Tea</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>OTSUKA, Yuuki</creatorcontrib><creatorcontrib>SHIMAMURA, Tomoko</creatorcontrib><creatorcontrib>SAKAJI, Michio</creatorcontrib><creatorcontrib>ARITA, Hikaru</creatorcontrib><creatorcontrib>KASHIWAGI, Takehiro</creatorcontrib><creatorcontrib>UKEDA, Hiroyuki</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical Sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>OTSUKA, Yuuki</au><au>SHIMAMURA, Tomoko</au><au>SAKAJI, Michio</au><au>ARITA, Hikaru</au><au>KASHIWAGI, Takehiro</au><au>UKEDA, Hiroyuki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quantification of Proline-containing Cyclic Dipeptides by LC-MS/MS</atitle><jtitle>Analytical Sciences</jtitle><stitle>ANAL. 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| subjects | Anaerobic processes Analytical Chemistry Chemistry Chromatography, Liquid - methods Cyclic dipeptide Cyclic dipeptides Fermentation Fermented food LC-MS/MS Limit of Detection Peptides, Cyclic - analysis Peptides, Cyclic - chemistry post-fermented tea Pro-containing DKPs Proline Proline - chemistry Tandem Mass Spectrometry - methods Tea |
| title | Quantification of Proline-containing Cyclic Dipeptides by LC-MS/MS |
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