Unusual deprivation of compatible solutes in Acinetobacter baumannii
Summary The opportunistic human pathogen Acinetobacter baumannii is one of the leading causes of nosocomial infections. The high prevalence of multidrug‐resistant strains, a high adaptability to changing environments and an overall pronounced stress resistance contribute to persistence and spread of...
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| Veröffentlicht in: | Environmental microbiology 2020-04, Vol.22 (4), p.1370-1380 |
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| creator | Zeidler, Sabine Müller, Volker |
| description | Summary
The opportunistic human pathogen Acinetobacter baumannii is one of the leading causes of nosocomial infections. The high prevalence of multidrug‐resistant strains, a high adaptability to changing environments and an overall pronounced stress resistance contribute to persistence and spread of the bacteria in hospitals and thereby promote repeated outbreaks. Altogether, the success of A. baumannii is mainly built on adaptation and stress resistance mechanisms, rather than relying on ‘true’ virulence factors. One of the stress factors that pathogens must cope with is osmolarity, which can differ between the external environment and different body parts of the human host. A. baumannii ATCC 19606T accumulates the compatible solutes glutamate, mannitol and trehalose in response to high salinities. In this work, it was found that most of the solutes vanish immediately after reaching stationary phase, a very unusual phenomenon. While glutamate can be metabolized, mannitol produced by MtlD is excreted to the medium in high amounts. First results indicate that A. baumannii ATCC 19606T undergoes a rapid switch to a dormant state (viable but non‐culturable) after disappearance of the compatible solutes. Resuscitation from this state could easily be achieved in PBS or fresh medium. |
| doi_str_mv | 10.1111/1462-2920.14951 |
| format | Article |
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The opportunistic human pathogen Acinetobacter baumannii is one of the leading causes of nosocomial infections. The high prevalence of multidrug‐resistant strains, a high adaptability to changing environments and an overall pronounced stress resistance contribute to persistence and spread of the bacteria in hospitals and thereby promote repeated outbreaks. Altogether, the success of A. baumannii is mainly built on adaptation and stress resistance mechanisms, rather than relying on ‘true’ virulence factors. One of the stress factors that pathogens must cope with is osmolarity, which can differ between the external environment and different body parts of the human host. A. baumannii ATCC 19606T accumulates the compatible solutes glutamate, mannitol and trehalose in response to high salinities. In this work, it was found that most of the solutes vanish immediately after reaching stationary phase, a very unusual phenomenon. While glutamate can be metabolized, mannitol produced by MtlD is excreted to the medium in high amounts. First results indicate that A. baumannii ATCC 19606T undergoes a rapid switch to a dormant state (viable but non‐culturable) after disappearance of the compatible solutes. Resuscitation from this state could easily be achieved in PBS or fresh medium.</description><identifier>ISSN: 1462-2912</identifier><identifier>EISSN: 1462-2920</identifier><identifier>DOI: 10.1111/1462-2920.14951</identifier><identifier>PMID: 32079040</identifier><language>eng</language><publisher>Hoboken, USA: John Wiley & Sons, Inc</publisher><subject>Acinetobacter baumannii ; Acinetobacter baumannii - metabolism ; Adaptability ; Adaptation ; Body parts ; Changing environments ; Compatibility ; Deprivation ; Dormancy ; Environmental changes ; Glutamic Acid - metabolism ; Mannitol ; Mannitol - metabolism ; Microbiological strains ; Nosocomial infection ; Opportunist infection ; Osmolarity ; Osmotic Pressure ; Pathogens ; Resistance mechanisms ; Resuscitation ; Solutes ; Stationary phase ; Trehalose ; Virulence ; Virulence factors</subject><ispartof>Environmental microbiology, 2020-04, Vol.22 (4), p.1370-1380</ispartof><rights>2020 The Authors. published by Society for Applied Microbiology and John Wiley & Sons Ltd.</rights><rights>2020 The Authors. Environmental Microbiology published by Society for Applied Microbiology and John Wiley & Sons Ltd.</rights><rights>2020 Society for Applied Microbiology and John Wiley & Sons Ltd</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3711-21ec1dac4ea1c6bb327e4444bea7bc5f15a7b1fdc870d8a7f92e70adc68fba2c3</citedby><cites>FETCH-LOGICAL-c3711-21ec1dac4ea1c6bb327e4444bea7bc5f15a7b1fdc870d8a7f92e70adc68fba2c3</cites><orcidid>0000-0001-7955-5508</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2F1462-2920.14951$$EPDF$$P50$$Gwiley$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2F1462-2920.14951$$EHTML$$P50$$Gwiley$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32079040$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zeidler, Sabine</creatorcontrib><creatorcontrib>Müller, Volker</creatorcontrib><title>Unusual deprivation of compatible solutes in Acinetobacter baumannii</title><title>Environmental microbiology</title><addtitle>Environ Microbiol</addtitle><description>Summary
The opportunistic human pathogen Acinetobacter baumannii is one of the leading causes of nosocomial infections. The high prevalence of multidrug‐resistant strains, a high adaptability to changing environments and an overall pronounced stress resistance contribute to persistence and spread of the bacteria in hospitals and thereby promote repeated outbreaks. Altogether, the success of A. baumannii is mainly built on adaptation and stress resistance mechanisms, rather than relying on ‘true’ virulence factors. One of the stress factors that pathogens must cope with is osmolarity, which can differ between the external environment and different body parts of the human host. A. baumannii ATCC 19606T accumulates the compatible solutes glutamate, mannitol and trehalose in response to high salinities. In this work, it was found that most of the solutes vanish immediately after reaching stationary phase, a very unusual phenomenon. While glutamate can be metabolized, mannitol produced by MtlD is excreted to the medium in high amounts. First results indicate that A. baumannii ATCC 19606T undergoes a rapid switch to a dormant state (viable but non‐culturable) after disappearance of the compatible solutes. Resuscitation from this state could easily be achieved in PBS or fresh medium.</description><subject>Acinetobacter baumannii</subject><subject>Acinetobacter baumannii - metabolism</subject><subject>Adaptability</subject><subject>Adaptation</subject><subject>Body parts</subject><subject>Changing environments</subject><subject>Compatibility</subject><subject>Deprivation</subject><subject>Dormancy</subject><subject>Environmental changes</subject><subject>Glutamic Acid - metabolism</subject><subject>Mannitol</subject><subject>Mannitol - metabolism</subject><subject>Microbiological strains</subject><subject>Nosocomial infection</subject><subject>Opportunist infection</subject><subject>Osmolarity</subject><subject>Osmotic Pressure</subject><subject>Pathogens</subject><subject>Resistance mechanisms</subject><subject>Resuscitation</subject><subject>Solutes</subject><subject>Stationary phase</subject><subject>Trehalose</subject><subject>Virulence</subject><subject>Virulence factors</subject><issn>1462-2912</issn><issn>1462-2920</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>WIN</sourceid><sourceid>EIF</sourceid><recordid>eNqFkD1PwzAQhi0EoqUws6FILCyhtuPGyViV8iEVsdDZsp2L5CqxSxyD-u9xSenAwi13Zz33ynoQuib4nsSaEpbTlJY0rqyckRM0Pr6cHmdCR-jC-w3GhGccn6NRRjEvMcNj9LC2wQfZJBVsO_Mpe-Ns4upEu3YbF9VA4l0TevCJsclcGwu9U1L30CVKhlZaa8wlOqtl4-Hq0Cdo_bh8Xzynq7enl8V8leqME5JSAppUUjOQROdKZZQDi6VAcqVnNZnFTupKFxxXheR1SYFjWem8qJWkOpuguyF327mPAL4XrfEamkZacMELmuUlIzljOKK3f9CNC52Nv4tUwYqMFSWN1HSgdOe876AWUUIru50gWOwFi71CsdcpfgTHi5tDblAtVEf-12gEZgPwZRrY_Zcnlq8vQ_A3OK-FSg</recordid><startdate>202004</startdate><enddate>202004</enddate><creator>Zeidler, Sabine</creator><creator>Müller, Volker</creator><general>John Wiley & Sons, Inc</general><general>Wiley Subscription Services, Inc</general><scope>24P</scope><scope>WIN</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QH</scope><scope>7QL</scope><scope>7ST</scope><scope>7T7</scope><scope>7TN</scope><scope>7U9</scope><scope>7UA</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H94</scope><scope>H95</scope><scope>H97</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope><scope>SOI</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-7955-5508</orcidid></search><sort><creationdate>202004</creationdate><title>Unusual deprivation of compatible solutes in Acinetobacter baumannii</title><author>Zeidler, Sabine ; Müller, Volker</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3711-21ec1dac4ea1c6bb327e4444bea7bc5f15a7b1fdc870d8a7f92e70adc68fba2c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Acinetobacter baumannii</topic><topic>Acinetobacter baumannii - metabolism</topic><topic>Adaptability</topic><topic>Adaptation</topic><topic>Body parts</topic><topic>Changing environments</topic><topic>Compatibility</topic><topic>Deprivation</topic><topic>Dormancy</topic><topic>Environmental changes</topic><topic>Glutamic Acid - metabolism</topic><topic>Mannitol</topic><topic>Mannitol - metabolism</topic><topic>Microbiological strains</topic><topic>Nosocomial infection</topic><topic>Opportunist infection</topic><topic>Osmolarity</topic><topic>Osmotic Pressure</topic><topic>Pathogens</topic><topic>Resistance mechanisms</topic><topic>Resuscitation</topic><topic>Solutes</topic><topic>Stationary phase</topic><topic>Trehalose</topic><topic>Virulence</topic><topic>Virulence factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zeidler, Sabine</creatorcontrib><creatorcontrib>Müller, Volker</creatorcontrib><collection>Wiley-Blackwell Open Access Titles</collection><collection>Wiley Free Content</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aqualine</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Oceanic Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Water Resources Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Environmental microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zeidler, Sabine</au><au>Müller, Volker</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Unusual deprivation of compatible solutes in Acinetobacter baumannii</atitle><jtitle>Environmental microbiology</jtitle><addtitle>Environ Microbiol</addtitle><date>2020-04</date><risdate>2020</risdate><volume>22</volume><issue>4</issue><spage>1370</spage><epage>1380</epage><pages>1370-1380</pages><issn>1462-2912</issn><eissn>1462-2920</eissn><abstract>Summary
The opportunistic human pathogen Acinetobacter baumannii is one of the leading causes of nosocomial infections. The high prevalence of multidrug‐resistant strains, a high adaptability to changing environments and an overall pronounced stress resistance contribute to persistence and spread of the bacteria in hospitals and thereby promote repeated outbreaks. Altogether, the success of A. baumannii is mainly built on adaptation and stress resistance mechanisms, rather than relying on ‘true’ virulence factors. One of the stress factors that pathogens must cope with is osmolarity, which can differ between the external environment and different body parts of the human host. A. baumannii ATCC 19606T accumulates the compatible solutes glutamate, mannitol and trehalose in response to high salinities. In this work, it was found that most of the solutes vanish immediately after reaching stationary phase, a very unusual phenomenon. While glutamate can be metabolized, mannitol produced by MtlD is excreted to the medium in high amounts. First results indicate that A. baumannii ATCC 19606T undergoes a rapid switch to a dormant state (viable but non‐culturable) after disappearance of the compatible solutes. Resuscitation from this state could easily be achieved in PBS or fresh medium.</abstract><cop>Hoboken, USA</cop><pub>John Wiley & Sons, Inc</pub><pmid>32079040</pmid><doi>10.1111/1462-2920.14951</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0001-7955-5508</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Acinetobacter baumannii Acinetobacter baumannii - metabolism Adaptability Adaptation Body parts Changing environments Compatibility Deprivation Dormancy Environmental changes Glutamic Acid - metabolism Mannitol Mannitol - metabolism Microbiological strains Nosocomial infection Opportunist infection Osmolarity Osmotic Pressure Pathogens Resistance mechanisms Resuscitation Solutes Stationary phase Trehalose Virulence Virulence factors |
| title | Unusual deprivation of compatible solutes in Acinetobacter baumannii |
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