Diurnal rhythm expression of transcription factor NF-κB subunit p65 in Pelteobagrus vachellii
The pleiotropic transcription factor nuclear factor-kappa B (NF-κB) has important functions in viral resistance. In the present study, we isolated a p65 subunit of NF-κB cDNA from Pelteobagrus vachellii (termed Pvp65) and characterized it. The full-length p65 cDNA comprised 3651 bp, including a 148-...
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| Veröffentlicht in: | Fish & shellfish immunology 2020-04, Vol.99, p.572-577 |
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| creator | Qin, Chuanjie Wang, Juanjuan Shi, Qinchao Lv, Yunyun Wan, Jun He, Yang Hu, Peng |
| description | The pleiotropic transcription factor nuclear factor-kappa B (NF-κB) has important functions in viral resistance. In the present study, we isolated a p65 subunit of NF-κB cDNA from Pelteobagrus vachellii (termed Pvp65) and characterized it. The full-length p65 cDNA comprised 3651 bp, including a 148-bp 5′-untranslated region (UTR), a 106-bp 3′-UTR, and an open reading frame encoding a 1067-amino acid putative protein. The protein sequence comprised a DNA binding motif, a Rel-homology domain, a Rel protein signature, a putative transcription activation domain, a nuclear localization signal, and a transcription initiation factor IIA domain. The expression of Pvp65 displayed a daily rhythm, with an acrophase at approximately at 15:32 h in the liver, 11:34 h in the spleen, and 16:45 h in the head kidney. In addition, infection with Aeromonas hydrophila caused Pvp65 expression to increase significantly (P |
| doi_str_mv | 10.1016/j.fsi.2020.02.048 |
| format | Article |
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•The cDNA of transcription factor NF-κB subunit p65 in P. vachellii was identified and analyzed.•NF-κB subunit p65 mRNA was ubiquitously expressed in P. vachellii tissues.•NF-κB subunit p65 showed obvious daily rhythm in liver, spleen, head kidney.•NF-κB subunit p65 was induced after bacterial challenge.</description><identifier>ISSN: 1050-4648</identifier><identifier>EISSN: 1095-9947</identifier><identifier>DOI: 10.1016/j.fsi.2020.02.048</identifier><identifier>PMID: 32112890</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Circadian rhythm ; Immunity ; Nuclear factor-kappa B ; p65 subunit ; Pelteobagrus vachellii</subject><ispartof>Fish & shellfish immunology, 2020-04, Vol.99, p.572-577</ispartof><rights>2020 Elsevier Ltd</rights><rights>Copyright © 2020 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c353t-46a17218bbfedfd4f3fd8ea508122039fb029c091106d30a4ef865c0157271cd3</citedby><cites>FETCH-LOGICAL-c353t-46a17218bbfedfd4f3fd8ea508122039fb029c091106d30a4ef865c0157271cd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1050464820301352$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32112890$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Qin, Chuanjie</creatorcontrib><creatorcontrib>Wang, Juanjuan</creatorcontrib><creatorcontrib>Shi, Qinchao</creatorcontrib><creatorcontrib>Lv, Yunyun</creatorcontrib><creatorcontrib>Wan, Jun</creatorcontrib><creatorcontrib>He, Yang</creatorcontrib><creatorcontrib>Hu, Peng</creatorcontrib><title>Diurnal rhythm expression of transcription factor NF-κB subunit p65 in Pelteobagrus vachellii</title><title>Fish & shellfish immunology</title><addtitle>Fish Shellfish Immunol</addtitle><description>The pleiotropic transcription factor nuclear factor-kappa B (NF-κB) has important functions in viral resistance. In the present study, we isolated a p65 subunit of NF-κB cDNA from Pelteobagrus vachellii (termed Pvp65) and characterized it. The full-length p65 cDNA comprised 3651 bp, including a 148-bp 5′-untranslated region (UTR), a 106-bp 3′-UTR, and an open reading frame encoding a 1067-amino acid putative protein. The protein sequence comprised a DNA binding motif, a Rel-homology domain, a Rel protein signature, a putative transcription activation domain, a nuclear localization signal, and a transcription initiation factor IIA domain. The expression of Pvp65 displayed a daily rhythm, with an acrophase at approximately at 15:32 h in the liver, 11:34 h in the spleen, and 16:45 h in the head kidney. In addition, infection with Aeromonas hydrophila caused Pvp65 expression to increase significantly (P < 0.05), and peaking at 12 h post infection in the spleen, at 24 in the head kidney, and at 12 h in the liver. Thus, NF-κB expression might be under light/dark cycle control in P. vachellii, and may be involved in the immune response to A. hydrophila.
•The cDNA of transcription factor NF-κB subunit p65 in P. vachellii was identified and analyzed.•NF-κB subunit p65 mRNA was ubiquitously expressed in P. vachellii tissues.•NF-κB subunit p65 showed obvious daily rhythm in liver, spleen, head kidney.•NF-κB subunit p65 was induced after bacterial challenge.</description><subject>Circadian rhythm</subject><subject>Immunity</subject><subject>Nuclear factor-kappa B</subject><subject>p65 subunit</subject><subject>Pelteobagrus vachellii</subject><issn>1050-4648</issn><issn>1095-9947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp9kE1OwzAQhS0EglI4ABvkJZuEsZ2kiVhB-ZUqYAFbLMcZU1dpEmyngqtxCM5EqgJLVjMavff05iPkiEHMgGWni9h4G3PgEAOPIcm3yIhBkUZFkUy213sKUZIl-R7Z934BAJnIYJfsCc4YzwsYkZdL27tG1dTNP8J8SfG9c-i9bRvaGhqcarx2tgvrg1E6tI7eX0dfnxfU92Xf2EC7LKW2oY9YB2xL9ep6T1dKz7GurT0gO0bVHg9_5pg8X189TW-j2cPN3fR8FmmRijB0VGzCWV6WBitTJUaYKkeVQs44B1GYEnihoWAMskqAStDkWaqBpRM-YboSY3Kyye1c-9ajD3JpvR4qqAbb3ksusiIByBM-SNlGql3rvUMjO2eXyn1IBnKNVS7kgFWusUrgcsA6eI5_4vtyidWf45fjIDjbCHB4cmXRSa8tNhor61AHWbX2n_hvH8uJEQ</recordid><startdate>202004</startdate><enddate>202004</enddate><creator>Qin, Chuanjie</creator><creator>Wang, Juanjuan</creator><creator>Shi, Qinchao</creator><creator>Lv, Yunyun</creator><creator>Wan, Jun</creator><creator>He, Yang</creator><creator>Hu, Peng</creator><general>Elsevier Ltd</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>202004</creationdate><title>Diurnal rhythm expression of transcription factor NF-κB subunit p65 in Pelteobagrus vachellii</title><author>Qin, Chuanjie ; Wang, Juanjuan ; Shi, Qinchao ; Lv, Yunyun ; Wan, Jun ; He, Yang ; Hu, Peng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c353t-46a17218bbfedfd4f3fd8ea508122039fb029c091106d30a4ef865c0157271cd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Circadian rhythm</topic><topic>Immunity</topic><topic>Nuclear factor-kappa B</topic><topic>p65 subunit</topic><topic>Pelteobagrus vachellii</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Qin, Chuanjie</creatorcontrib><creatorcontrib>Wang, Juanjuan</creatorcontrib><creatorcontrib>Shi, Qinchao</creatorcontrib><creatorcontrib>Lv, Yunyun</creatorcontrib><creatorcontrib>Wan, Jun</creatorcontrib><creatorcontrib>He, Yang</creatorcontrib><creatorcontrib>Hu, Peng</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Fish & shellfish immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Qin, Chuanjie</au><au>Wang, Juanjuan</au><au>Shi, Qinchao</au><au>Lv, Yunyun</au><au>Wan, Jun</au><au>He, Yang</au><au>Hu, Peng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Diurnal rhythm expression of transcription factor NF-κB subunit p65 in Pelteobagrus vachellii</atitle><jtitle>Fish & shellfish immunology</jtitle><addtitle>Fish Shellfish Immunol</addtitle><date>2020-04</date><risdate>2020</risdate><volume>99</volume><spage>572</spage><epage>577</epage><pages>572-577</pages><issn>1050-4648</issn><eissn>1095-9947</eissn><abstract>The pleiotropic transcription factor nuclear factor-kappa B (NF-κB) has important functions in viral resistance. In the present study, we isolated a p65 subunit of NF-κB cDNA from Pelteobagrus vachellii (termed Pvp65) and characterized it. The full-length p65 cDNA comprised 3651 bp, including a 148-bp 5′-untranslated region (UTR), a 106-bp 3′-UTR, and an open reading frame encoding a 1067-amino acid putative protein. The protein sequence comprised a DNA binding motif, a Rel-homology domain, a Rel protein signature, a putative transcription activation domain, a nuclear localization signal, and a transcription initiation factor IIA domain. The expression of Pvp65 displayed a daily rhythm, with an acrophase at approximately at 15:32 h in the liver, 11:34 h in the spleen, and 16:45 h in the head kidney. In addition, infection with Aeromonas hydrophila caused Pvp65 expression to increase significantly (P < 0.05), and peaking at 12 h post infection in the spleen, at 24 in the head kidney, and at 12 h in the liver. Thus, NF-κB expression might be under light/dark cycle control in P. vachellii, and may be involved in the immune response to A. hydrophila.
•The cDNA of transcription factor NF-κB subunit p65 in P. vachellii was identified and analyzed.•NF-κB subunit p65 mRNA was ubiquitously expressed in P. vachellii tissues.•NF-κB subunit p65 showed obvious daily rhythm in liver, spleen, head kidney.•NF-κB subunit p65 was induced after bacterial challenge.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>32112890</pmid><doi>10.1016/j.fsi.2020.02.048</doi><tpages>6</tpages></addata></record> |
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| subjects | Circadian rhythm Immunity Nuclear factor-kappa B p65 subunit Pelteobagrus vachellii |
| title | Diurnal rhythm expression of transcription factor NF-κB subunit p65 in Pelteobagrus vachellii |
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