New engineered Geobacillus lipase GD-95RM for industry focusing on the cleaner production of fatty esters and household washing product formulations

This study presents a new microbial lipolytic enzyme GD-95RM designed via random mutagenesis using previously characterized GD-95 lipase as a template. The improvement in activity of GD-95 lipase was caused by E100K, F154V and V174I mutations. Compared with GD-95 lipase, the GD-95RM lipase had 1.3-f...

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Veröffentlicht in:	World journal of microbiology & biotechnology 2020-03, Vol.36 (3), p.41-41, Article 41
Hauptverfasser:	Druteika, Gytis, Sadauskas, Mikas, Malunavicius, Vilius, Lastauskiene, Egle, Statkeviciute, Roberta, Savickaite, Agne, Gudiukaite, Renata
Format:	Artikel
Sprache:	eng
Schlagworte:	Alcohols Animal fat Applied Microbiology Biochemistry Biomedical and Life Sciences Biotechnology Boric acid Chemical synthesis Citric acid Environmental Engineering/Biotechnology Esters Ethanol Fatty acids Fish oils Formulations High temperature Hydrolysis Life Sciences Lipase Microbiology Microorganisms Mutation Octyl oleate Oils & fats Original Paper Random mutagenesis Sodium chloride Sodium citrate Sodium perborate Substrates Transesterification Urea
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