Amniotic Membrane Enhance the Effect of Vascular Endothelial Growth Factor on the Angiogenic Marker Expression of Stem Cells from Human Exfoliated Deciduous Teeth
Previously, it was reported that human amniotic membrane (AM) induced stem cells from human deciduous exfoliated teeth (SHED) endothelial-like-cell differentiation. This interesting effect of AM matrix on SHED demands further elucidation. Objective of this in vitro work was to study the effect of 24...
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| Veröffentlicht in: | Applied biochemistry and biotechnology 2020-05, Vol.191 (1), p.177-190 |
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| creator | Yusof, Muhammad Fuad Hilmi Hashim, Siti Nurnasihah Md Zahari, Wafa’ Chandra, Hamshawagini Noordin, Khairul Bariah Ahmad Amin Kannan, Thirumulu Ponnuraj Hamid, Suzina Sheikh Abdul Mokhtar, Khairani Idah Azlina, Ahmad |
| description | Previously, it was reported that human amniotic membrane (AM) induced stem cells from human deciduous exfoliated teeth (SHED) endothelial-like-cell differentiation. This interesting effect of AM matrix on SHED demands further elucidation. Objective of this in vitro work was to study the effect of 24-h VEGF induced on SHED endothelial differentiation when seeded on acellular stromal side (SS) of AM matrix. Stemness of SHED was identified by flow cytometry. Cell attachment and morphological changes towards the matrix was observed by scanning electron microscopy. Protein expression of endothelial marker was examined by Western blot. The expression of stem cells and endothelial-specific gene markers of VEGF-induced SHED cultured on human AM was inspected via reverse transcriptase-polymerase chain reaction. Results showed SHED at both passages retain stemness property. Ang-1 protein was expressed in SHED. Cells treated with VEGF and cultured on AM transformed attached well to AM. VEGF-induced SHED expressed both stem cell and endothelial-specific markers throughout the treatments and timeline. Interestingly, prolonged VEGF treatment increased the expression of
Cox-2
and
VE-Cadherin
genes in all treated groups when compared to SHED. It was concluded that the VEGF-induced SHED showed better expression of endothelial-specific markers when cultured on SS of AM, with prolonged VEGF treatment. |
| doi_str_mv | 10.1007/s12010-020-03266-1 |
| format | Article |
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Cox-2
and
VE-Cadherin
genes in all treated groups when compared to SHED. It was concluded that the VEGF-induced SHED showed better expression of endothelial-specific markers when cultured on SS of AM, with prolonged VEGF treatment.</description><identifier>ISSN: 0273-2289</identifier><identifier>EISSN: 1559-0291</identifier><identifier>DOI: 10.1007/s12010-020-03266-1</identifier><identifier>PMID: 32096060</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Amnion - chemistry ; Amniotic membrane ; Angiogenesis ; Antigens, Differentiation - biosynthesis ; Biochemistry ; Biomarkers ; Biotechnology ; Cadherins ; Cell adhesion ; Cell differentiation ; Cells, Cultured ; Chemistry ; Chemistry and Materials Science ; Cyclooxygenase-2 ; Differentiation (biology) ; Extracellular matrix ; Extracellular Matrix - chemistry ; Flow cytometry ; Growth factors ; Humans ; Membranes ; Neovascularization, Physiologic - drug effects ; Polymerase chain reaction ; Proteins ; RNA-directed DNA polymerase ; Scanning electron microscopy ; Stem cells ; Stem Cells - cytology ; Stem Cells - metabolism ; Tissue engineering ; Tooth Exfoliation ; Tooth, Deciduous - cytology ; Tooth, Deciduous - metabolism ; Vascular endothelial growth factor ; Vascular Endothelial Growth Factor A - pharmacology</subject><ispartof>Applied biochemistry and biotechnology, 2020-05, Vol.191 (1), p.177-190</ispartof><rights>Springer Science+Business Media, LLC, part of Springer Nature 2020</rights><rights>Springer Science+Business Media, LLC, part of Springer Nature 2020.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c456t-8a01f81927e30eaf26917f466a1e9ad2a1e0904ad5b8b8cfd1dfd9fc5761c1983</citedby><cites>FETCH-LOGICAL-c456t-8a01f81927e30eaf26917f466a1e9ad2a1e0904ad5b8b8cfd1dfd9fc5761c1983</cites><orcidid>0000-0002-1393-5775</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12010-020-03266-1$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12010-020-03266-1$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32096060$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yusof, Muhammad Fuad Hilmi</creatorcontrib><creatorcontrib>Hashim, Siti Nurnasihah Md</creatorcontrib><creatorcontrib>Zahari, Wafa’</creatorcontrib><creatorcontrib>Chandra, Hamshawagini</creatorcontrib><creatorcontrib>Noordin, Khairul Bariah Ahmad Amin</creatorcontrib><creatorcontrib>Kannan, Thirumulu Ponnuraj</creatorcontrib><creatorcontrib>Hamid, Suzina Sheikh Abdul</creatorcontrib><creatorcontrib>Mokhtar, Khairani Idah</creatorcontrib><creatorcontrib>Azlina, Ahmad</creatorcontrib><title>Amniotic Membrane Enhance the Effect of Vascular Endothelial Growth Factor on the Angiogenic Marker Expression of Stem Cells from Human Exfoliated Deciduous Teeth</title><title>Applied biochemistry and biotechnology</title><addtitle>Appl Biochem Biotechnol</addtitle><addtitle>Appl Biochem Biotechnol</addtitle><description>Previously, it was reported that human amniotic membrane (AM) induced stem cells from human deciduous exfoliated teeth (SHED) endothelial-like-cell differentiation. This interesting effect of AM matrix on SHED demands further elucidation. Objective of this in vitro work was to study the effect of 24-h VEGF induced on SHED endothelial differentiation when seeded on acellular stromal side (SS) of AM matrix. Stemness of SHED was identified by flow cytometry. Cell attachment and morphological changes towards the matrix was observed by scanning electron microscopy. Protein expression of endothelial marker was examined by Western blot. The expression of stem cells and endothelial-specific gene markers of VEGF-induced SHED cultured on human AM was inspected via reverse transcriptase-polymerase chain reaction. Results showed SHED at both passages retain stemness property. Ang-1 protein was expressed in SHED. Cells treated with VEGF and cultured on AM transformed attached well to AM. VEGF-induced SHED expressed both stem cell and endothelial-specific markers throughout the treatments and timeline. Interestingly, prolonged VEGF treatment increased the expression of
Cox-2
and
VE-Cadherin
genes in all treated groups when compared to SHED. It was concluded that the VEGF-induced SHED showed better expression of endothelial-specific markers when cultured on SS of AM, with prolonged VEGF treatment.</description><subject>Amnion - chemistry</subject><subject>Amniotic membrane</subject><subject>Angiogenesis</subject><subject>Antigens, Differentiation - biosynthesis</subject><subject>Biochemistry</subject><subject>Biomarkers</subject><subject>Biotechnology</subject><subject>Cadherins</subject><subject>Cell adhesion</subject><subject>Cell differentiation</subject><subject>Cells, Cultured</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Cyclooxygenase-2</subject><subject>Differentiation (biology)</subject><subject>Extracellular matrix</subject><subject>Extracellular Matrix - chemistry</subject><subject>Flow cytometry</subject><subject>Growth factors</subject><subject>Humans</subject><subject>Membranes</subject><subject>Neovascularization, Physiologic - drug effects</subject><subject>Polymerase chain reaction</subject><subject>Proteins</subject><subject>RNA-directed DNA polymerase</subject><subject>Scanning electron microscopy</subject><subject>Stem cells</subject><subject>Stem Cells - cytology</subject><subject>Stem Cells - metabolism</subject><subject>Tissue engineering</subject><subject>Tooth Exfoliation</subject><subject>Tooth, Deciduous - cytology</subject><subject>Tooth, Deciduous - metabolism</subject><subject>Vascular endothelial growth factor</subject><subject>Vascular Endothelial Growth Factor A - pharmacology</subject><issn>0273-2289</issn><issn>1559-0291</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kc9u1DAQxi0EokvhBTggS1y4BMZO4sTH1dI_SEUcKFwtrzPeTUnsxXYEvA5PyrRbQOLAwRrL8_u-Gflj7LmA1wKge5OFBAEVSDq1VKoSD9hKtK2mJy0eshXIrq6k7PUJe5LzDYCQfds9Zie1BK1AwYr9XM9hjGV0_D3O22QD8rOwt8EhL3u6e4-u8Oj5Z5vdMtlE7SFSaxrtxC9S_Fb2_Ny6EhOP4U6zDrsx7jDcetr0BUny_ZAw55EAcvpYcOYbnKbMfYozv1xmG4jxkTwLDvwtunFY4pL5NWLZP2WPvJ0yPruvp-zT-dn15rK6-nDxbrO-qlzTqlL1FoTvhZYd1oDWS6VF5xulrEBtB0kFNDR2aLf9tnd-EIMftHdtp4QTuq9P2auj7yHFrwvmYuYxO9qTPoWWMbJWDTSq7iWhL_9Bb-KSAm1nZCNA17pvgSh5pFyKOSf05pDG2aYfRoC5TdAcEzSUoLlL0AgSvbi3XrYzDn8kvyMjoD4CmVphh-nv7P_Y_gIT9Keo</recordid><startdate>20200501</startdate><enddate>20200501</enddate><creator>Yusof, Muhammad Fuad Hilmi</creator><creator>Hashim, Siti Nurnasihah Md</creator><creator>Zahari, Wafa’</creator><creator>Chandra, Hamshawagini</creator><creator>Noordin, Khairul Bariah Ahmad Amin</creator><creator>Kannan, Thirumulu Ponnuraj</creator><creator>Hamid, Suzina Sheikh Abdul</creator><creator>Mokhtar, Khairani Idah</creator><creator>Azlina, Ahmad</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>SOI</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-1393-5775</orcidid></search><sort><creationdate>20200501</creationdate><title>Amniotic Membrane Enhance the Effect of Vascular Endothelial Growth Factor on the Angiogenic Marker Expression of Stem Cells from Human Exfoliated Deciduous Teeth</title><author>Yusof, Muhammad Fuad Hilmi ; Hashim, Siti Nurnasihah Md ; Zahari, Wafa’ ; Chandra, Hamshawagini ; Noordin, Khairul Bariah Ahmad Amin ; Kannan, Thirumulu Ponnuraj ; Hamid, Suzina Sheikh Abdul ; Mokhtar, Khairani Idah ; Azlina, Ahmad</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c456t-8a01f81927e30eaf26917f466a1e9ad2a1e0904ad5b8b8cfd1dfd9fc5761c1983</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Amnion - chemistry</topic><topic>Amniotic membrane</topic><topic>Angiogenesis</topic><topic>Antigens, Differentiation - biosynthesis</topic><topic>Biochemistry</topic><topic>Biomarkers</topic><topic>Biotechnology</topic><topic>Cadherins</topic><topic>Cell adhesion</topic><topic>Cell differentiation</topic><topic>Cells, Cultured</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Cyclooxygenase-2</topic><topic>Differentiation (biology)</topic><topic>Extracellular matrix</topic><topic>Extracellular Matrix - chemistry</topic><topic>Flow cytometry</topic><topic>Growth factors</topic><topic>Humans</topic><topic>Membranes</topic><topic>Neovascularization, Physiologic - drug effects</topic><topic>Polymerase chain reaction</topic><topic>Proteins</topic><topic>RNA-directed DNA polymerase</topic><topic>Scanning electron microscopy</topic><topic>Stem cells</topic><topic>Stem Cells - cytology</topic><topic>Stem Cells - metabolism</topic><topic>Tissue engineering</topic><topic>Tooth Exfoliation</topic><topic>Tooth, Deciduous - cytology</topic><topic>Tooth, Deciduous - metabolism</topic><topic>Vascular endothelial growth factor</topic><topic>Vascular Endothelial Growth Factor A - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yusof, Muhammad Fuad Hilmi</creatorcontrib><creatorcontrib>Hashim, Siti Nurnasihah Md</creatorcontrib><creatorcontrib>Zahari, Wafa’</creatorcontrib><creatorcontrib>Chandra, Hamshawagini</creatorcontrib><creatorcontrib>Noordin, Khairul Bariah Ahmad Amin</creatorcontrib><creatorcontrib>Kannan, Thirumulu Ponnuraj</creatorcontrib><creatorcontrib>Hamid, Suzina Sheikh Abdul</creatorcontrib><creatorcontrib>Mokhtar, Khairani Idah</creatorcontrib><creatorcontrib>Azlina, Ahmad</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Applied biochemistry and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yusof, Muhammad Fuad Hilmi</au><au>Hashim, Siti Nurnasihah Md</au><au>Zahari, Wafa’</au><au>Chandra, Hamshawagini</au><au>Noordin, Khairul Bariah Ahmad Amin</au><au>Kannan, Thirumulu Ponnuraj</au><au>Hamid, Suzina Sheikh Abdul</au><au>Mokhtar, Khairani Idah</au><au>Azlina, Ahmad</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Amniotic Membrane Enhance the Effect of Vascular Endothelial Growth Factor on the Angiogenic Marker Expression of Stem Cells from Human Exfoliated Deciduous Teeth</atitle><jtitle>Applied biochemistry and biotechnology</jtitle><stitle>Appl Biochem Biotechnol</stitle><addtitle>Appl Biochem Biotechnol</addtitle><date>2020-05-01</date><risdate>2020</risdate><volume>191</volume><issue>1</issue><spage>177</spage><epage>190</epage><pages>177-190</pages><issn>0273-2289</issn><eissn>1559-0291</eissn><abstract>Previously, it was reported that human amniotic membrane (AM) induced stem cells from human deciduous exfoliated teeth (SHED) endothelial-like-cell differentiation. This interesting effect of AM matrix on SHED demands further elucidation. Objective of this in vitro work was to study the effect of 24-h VEGF induced on SHED endothelial differentiation when seeded on acellular stromal side (SS) of AM matrix. Stemness of SHED was identified by flow cytometry. Cell attachment and morphological changes towards the matrix was observed by scanning electron microscopy. Protein expression of endothelial marker was examined by Western blot. The expression of stem cells and endothelial-specific gene markers of VEGF-induced SHED cultured on human AM was inspected via reverse transcriptase-polymerase chain reaction. Results showed SHED at both passages retain stemness property. Ang-1 protein was expressed in SHED. Cells treated with VEGF and cultured on AM transformed attached well to AM. VEGF-induced SHED expressed both stem cell and endothelial-specific markers throughout the treatments and timeline. Interestingly, prolonged VEGF treatment increased the expression of
Cox-2
and
VE-Cadherin
genes in all treated groups when compared to SHED. It was concluded that the VEGF-induced SHED showed better expression of endothelial-specific markers when cultured on SS of AM, with prolonged VEGF treatment.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>32096060</pmid><doi>10.1007/s12010-020-03266-1</doi><tpages>14</tpages><orcidid>https://orcid.org/0000-0002-1393-5775</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Applied biochemistry and biotechnology, 2020-05, Vol.191 (1), p.177-190 |
| issn | 0273-2289 1559-0291 |
| language | eng |
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| subjects | Amnion - chemistry Amniotic membrane Angiogenesis Antigens, Differentiation - biosynthesis Biochemistry Biomarkers Biotechnology Cadherins Cell adhesion Cell differentiation Cells, Cultured Chemistry Chemistry and Materials Science Cyclooxygenase-2 Differentiation (biology) Extracellular matrix Extracellular Matrix - chemistry Flow cytometry Growth factors Humans Membranes Neovascularization, Physiologic - drug effects Polymerase chain reaction Proteins RNA-directed DNA polymerase Scanning electron microscopy Stem cells Stem Cells - cytology Stem Cells - metabolism Tissue engineering Tooth Exfoliation Tooth, Deciduous - cytology Tooth, Deciduous - metabolism Vascular endothelial growth factor Vascular Endothelial Growth Factor A - pharmacology |
| title | Amniotic Membrane Enhance the Effect of Vascular Endothelial Growth Factor on the Angiogenic Marker Expression of Stem Cells from Human Exfoliated Deciduous Teeth |
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