Amperometric Bioplatforms To Detect Regional DNA Methylation with Single-Base Sensitivity

This work reports the first bioplatform able to determine electrochemically 5-hydroxymethylcytosine (5-hmC) methylation events at localized sites and single-base sensitivity. The described bioplatform relies on a specific antibody (anti-5-hmC), further conjugated with commercial bioreagents loaded w...

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Veröffentlicht in:	Analytical chemistry (Washington) 2020-04, Vol.92 (7), p.5604-5612
Hauptverfasser:	Povedano, Eloy, Ruiz-Valdepeñas Montiel, Víctor, Gamella, Maria, Pedrero, María, Barderas, Rodrigo, Peláez-García, Alberto, Mendiola, Marta, Hardisson, David, Feliú, Jaime, Yáñez-Sedeño, Paloma, Campuzano, Susana, Pingarrón, José M
Format:	Artikel
Sprache:	eng
Schlagworte:	5-Methylcytosine - analogs & derivatives 5-Methylcytosine - metabolism Amperometry Amplification Analytical chemistry Antibodies Biosensors Cancer Cell Line, Tumor Chemistry Colorectal carcinoma Complementary DNA Deoxyribonucleic acid DNA DNA Methylation Electrical measurement Electrochemical analysis Electrochemistry Electrochemistry - methods Electrodes Genomes Horseradish peroxidase Humans Hybridization Hydrogen peroxide Hydroquinone Limit of Detection Microspheres Nanoparticles Nucleotide sequence Nucleotides Peroxidase Sensitivity Streptavidin Tumor suppressor genes
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