Preliminary Comparative Assessment of Brucellergene Skin Test for Diagnosis of Brucellosis in Dromedary Camels (Camelus dromedarius)
This study was conducted to evaluate the use of Brucellergene skin test (BST) for the diagnosis of Brucellosis in camels ( Camelus dromedarius ) in comparison with Rose Bengal test (RBT) and competitive enzyme-linked immunosorbent assay (c-ELISA). A total of 68 apparently healthy adult dromedary cam...
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| Veröffentlicht in: | Vector borne and zoonotic diseases (Larchmont, N.Y.) N.Y.), 2020-06, Vol.20 (6), p.412-417 |
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| creator | Khalafalla, Abdelmalik Ibrahim Rashid, Javed Khan, Rashid A Alamin, Khalafallah M Benkhelil, Abdelghani De Massis, Fabrizio Calistri, Paolo Giovannini, Armando Khan, Irfan A Al Hosani, Mohamed A Al Muhairi, Salama S |
| description | This study was conducted to evaluate the use of Brucellergene skin test (BST) for the diagnosis of Brucellosis in camels (
Camelus dromedarius
) in comparison with Rose Bengal test (RBT) and competitive enzyme-linked immunosorbent assay (c-ELISA). A total of 68 apparently healthy adult dromedary camels of either gender from three different geographical locations of Abu Dhabi Emirate, United Arab Emirates (UAE), were included in the study. The skin test was applied on two shaved areas at the middle of the neck: one for the test and the other area was injected with normal saline as a control. Reading was done 72 h postinjection. Results were subjected to Bayesian analysis to assess the test performances in camels. The model estimated the following sensitivity and specificity median values: BST: Se = 70.72%, Sp = 98.82%; RBT: Se = 93.27%, Sp = 97.79%; and c-ELISA: Se = 94.78%, Sp = 98.48%. As the BST investigated in this study proved to be a highly specific test, we propose using it as a confirmatory test in camels particularly when the serological tests give doubtful results on individual animals. |
| doi_str_mv | 10.1089/vbz.2019.2537 |
| format | Article |
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Camelus dromedarius
) in comparison with Rose Bengal test (RBT) and competitive enzyme-linked immunosorbent assay (c-ELISA). A total of 68 apparently healthy adult dromedary camels of either gender from three different geographical locations of Abu Dhabi Emirate, United Arab Emirates (UAE), were included in the study. The skin test was applied on two shaved areas at the middle of the neck: one for the test and the other area was injected with normal saline as a control. Reading was done 72 h postinjection. Results were subjected to Bayesian analysis to assess the test performances in camels. The model estimated the following sensitivity and specificity median values: BST: Se = 70.72%, Sp = 98.82%; RBT: Se = 93.27%, Sp = 97.79%; and c-ELISA: Se = 94.78%, Sp = 98.48%. As the BST investigated in this study proved to be a highly specific test, we propose using it as a confirmatory test in camels particularly when the serological tests give doubtful results on individual animals.</description><identifier>ISSN: 1530-3667</identifier><identifier>EISSN: 1557-7759</identifier><identifier>DOI: 10.1089/vbz.2019.2537</identifier><identifier>PMID: 32077807</identifier><language>eng</language><publisher>United States: Mary Ann Liebert, Inc., publishers</publisher><subject>Animals ; Bayesian analysis ; Brucellosis ; Brucellosis - diagnosis ; Brucellosis - microbiology ; Brucellosis - veterinary ; Camelidae ; Camels ; Camelus - microbiology ; Camelus dromedarius ; Diagnosis ; Enzyme-linked immunosorbent assay ; Enzyme-Linked Immunosorbent Assay - veterinary ; Geographical locations ; Original Articles ; Rose Bengal ; Sensitivity and Specificity ; Serologic Tests - veterinary ; Serological tests ; Skin tests ; Skin Tests - methods ; Skin Tests - veterinary</subject><ispartof>Vector borne and zoonotic diseases (Larchmont, N.Y.), 2020-06, Vol.20 (6), p.412-417</ispartof><rights>2020, Mary Ann Liebert, Inc., publishers</rights><rights>Copyright Mary Ann Liebert, Inc. Jun 2020</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c365t-65c0a0b22908035e8ad45bdd494ba7d5f69c95d9e58c29781612fb3e216de1373</citedby><cites>FETCH-LOGICAL-c365t-65c0a0b22908035e8ad45bdd494ba7d5f69c95d9e58c29781612fb3e216de1373</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32077807$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Khalafalla, Abdelmalik Ibrahim</creatorcontrib><creatorcontrib>Rashid, Javed</creatorcontrib><creatorcontrib>Khan, Rashid A</creatorcontrib><creatorcontrib>Alamin, Khalafallah M</creatorcontrib><creatorcontrib>Benkhelil, Abdelghani</creatorcontrib><creatorcontrib>De Massis, Fabrizio</creatorcontrib><creatorcontrib>Calistri, Paolo</creatorcontrib><creatorcontrib>Giovannini, Armando</creatorcontrib><creatorcontrib>Khan, Irfan A</creatorcontrib><creatorcontrib>Al Hosani, Mohamed A</creatorcontrib><creatorcontrib>Al Muhairi, Salama S</creatorcontrib><title>Preliminary Comparative Assessment of Brucellergene Skin Test for Diagnosis of Brucellosis in Dromedary Camels (Camelus dromedarius)</title><title>Vector borne and zoonotic diseases (Larchmont, N.Y.)</title><addtitle>Vector Borne Zoonotic Dis</addtitle><description>This study was conducted to evaluate the use of Brucellergene skin test (BST) for the diagnosis of Brucellosis in camels (
Camelus dromedarius
) in comparison with Rose Bengal test (RBT) and competitive enzyme-linked immunosorbent assay (c-ELISA). A total of 68 apparently healthy adult dromedary camels of either gender from three different geographical locations of Abu Dhabi Emirate, United Arab Emirates (UAE), were included in the study. The skin test was applied on two shaved areas at the middle of the neck: one for the test and the other area was injected with normal saline as a control. Reading was done 72 h postinjection. Results were subjected to Bayesian analysis to assess the test performances in camels. The model estimated the following sensitivity and specificity median values: BST: Se = 70.72%, Sp = 98.82%; RBT: Se = 93.27%, Sp = 97.79%; and c-ELISA: Se = 94.78%, Sp = 98.48%. As the BST investigated in this study proved to be a highly specific test, we propose using it as a confirmatory test in camels particularly when the serological tests give doubtful results on individual animals.</description><subject>Animals</subject><subject>Bayesian analysis</subject><subject>Brucellosis</subject><subject>Brucellosis - diagnosis</subject><subject>Brucellosis - microbiology</subject><subject>Brucellosis - veterinary</subject><subject>Camelidae</subject><subject>Camels</subject><subject>Camelus - microbiology</subject><subject>Camelus dromedarius</subject><subject>Diagnosis</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Enzyme-Linked Immunosorbent Assay - veterinary</subject><subject>Geographical locations</subject><subject>Original Articles</subject><subject>Rose Bengal</subject><subject>Sensitivity and Specificity</subject><subject>Serologic Tests - veterinary</subject><subject>Serological tests</subject><subject>Skin tests</subject><subject>Skin Tests - methods</subject><subject>Skin Tests - veterinary</subject><issn>1530-3667</issn><issn>1557-7759</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtv1DAURi0EoqWwZIsssSmLDH7Esb1sp7ykSiBR1paT3FQuiT34JpVgzQ_HmRkQYsPq-nH06bs6hDznbMOZsa_v2x8bwbjdCCX1A3LKldKV1so-XM-SVbJp9Al5gnjHmOCGq8fkRAqmtWH6lPz8lGEMU4g-f6fbNO189nO4B3qBCIgTxJmmgV7mpYNxhHwLEejnryHSG8CZDinTq-BvY8KAf4H7a4Gucpqg32f7CUak5_u5IO2PP2HBV0_Jo8GPCM-O84x8efvmZvu-uv747sP24rrqZKPmqlEd86wVwjLDpALj-1q1fV_buvW6V0NjO6t6C8p0wmrDGy6GVoLgTQ9canlGzg-5u5y-LaW_mwKudX2EtKATUllplTV1QV_-g96lJcfSzomaGcF1I2ShqgPV5YSYYXC7HKayruPMrXpc0eNWPW7VU_gXx9SlLdv_oX_7KIA8AOuzj3EM0EKe_xP7C9gdnYw</recordid><startdate>20200601</startdate><enddate>20200601</enddate><creator>Khalafalla, Abdelmalik Ibrahim</creator><creator>Rashid, Javed</creator><creator>Khan, Rashid A</creator><creator>Alamin, Khalafallah M</creator><creator>Benkhelil, Abdelghani</creator><creator>De Massis, Fabrizio</creator><creator>Calistri, Paolo</creator><creator>Giovannini, Armando</creator><creator>Khan, Irfan A</creator><creator>Al Hosani, Mohamed A</creator><creator>Al Muhairi, Salama S</creator><general>Mary Ann Liebert, Inc., publishers</general><general>Mary Ann Liebert, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7SS</scope><scope>7U9</scope><scope>C1K</scope><scope>H94</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>20200601</creationdate><title>Preliminary Comparative Assessment of Brucellergene Skin Test for Diagnosis of Brucellosis in Dromedary Camels (Camelus dromedarius)</title><author>Khalafalla, Abdelmalik Ibrahim ; Rashid, Javed ; Khan, Rashid A ; Alamin, Khalafallah M ; Benkhelil, Abdelghani ; De Massis, Fabrizio ; Calistri, Paolo ; Giovannini, Armando ; Khan, Irfan A ; Al Hosani, Mohamed A ; Al Muhairi, Salama S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c365t-65c0a0b22908035e8ad45bdd494ba7d5f69c95d9e58c29781612fb3e216de1373</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Animals</topic><topic>Bayesian analysis</topic><topic>Brucellosis</topic><topic>Brucellosis - diagnosis</topic><topic>Brucellosis - microbiology</topic><topic>Brucellosis - veterinary</topic><topic>Camelidae</topic><topic>Camels</topic><topic>Camelus - microbiology</topic><topic>Camelus dromedarius</topic><topic>Diagnosis</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Enzyme-Linked Immunosorbent Assay - veterinary</topic><topic>Geographical locations</topic><topic>Original Articles</topic><topic>Rose Bengal</topic><topic>Sensitivity and Specificity</topic><topic>Serologic Tests - veterinary</topic><topic>Serological tests</topic><topic>Skin tests</topic><topic>Skin Tests - methods</topic><topic>Skin Tests - veterinary</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Khalafalla, Abdelmalik Ibrahim</creatorcontrib><creatorcontrib>Rashid, Javed</creatorcontrib><creatorcontrib>Khan, Rashid A</creatorcontrib><creatorcontrib>Alamin, Khalafallah M</creatorcontrib><creatorcontrib>Benkhelil, Abdelghani</creatorcontrib><creatorcontrib>De Massis, Fabrizio</creatorcontrib><creatorcontrib>Calistri, Paolo</creatorcontrib><creatorcontrib>Giovannini, Armando</creatorcontrib><creatorcontrib>Khan, Irfan A</creatorcontrib><creatorcontrib>Al Hosani, Mohamed A</creatorcontrib><creatorcontrib>Al Muhairi, Salama S</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Virology and AIDS Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Vector borne and zoonotic diseases (Larchmont, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Khalafalla, Abdelmalik Ibrahim</au><au>Rashid, Javed</au><au>Khan, Rashid A</au><au>Alamin, Khalafallah M</au><au>Benkhelil, Abdelghani</au><au>De Massis, Fabrizio</au><au>Calistri, Paolo</au><au>Giovannini, Armando</au><au>Khan, Irfan A</au><au>Al Hosani, Mohamed A</au><au>Al Muhairi, Salama S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preliminary Comparative Assessment of Brucellergene Skin Test for Diagnosis of Brucellosis in Dromedary Camels (Camelus dromedarius)</atitle><jtitle>Vector borne and zoonotic diseases (Larchmont, N.Y.)</jtitle><addtitle>Vector Borne Zoonotic Dis</addtitle><date>2020-06-01</date><risdate>2020</risdate><volume>20</volume><issue>6</issue><spage>412</spage><epage>417</epage><pages>412-417</pages><issn>1530-3667</issn><eissn>1557-7759</eissn><abstract>This study was conducted to evaluate the use of Brucellergene skin test (BST) for the diagnosis of Brucellosis in camels (
Camelus dromedarius
) in comparison with Rose Bengal test (RBT) and competitive enzyme-linked immunosorbent assay (c-ELISA). A total of 68 apparently healthy adult dromedary camels of either gender from three different geographical locations of Abu Dhabi Emirate, United Arab Emirates (UAE), were included in the study. The skin test was applied on two shaved areas at the middle of the neck: one for the test and the other area was injected with normal saline as a control. Reading was done 72 h postinjection. Results were subjected to Bayesian analysis to assess the test performances in camels. The model estimated the following sensitivity and specificity median values: BST: Se = 70.72%, Sp = 98.82%; RBT: Se = 93.27%, Sp = 97.79%; and c-ELISA: Se = 94.78%, Sp = 98.48%. As the BST investigated in this study proved to be a highly specific test, we propose using it as a confirmatory test in camels particularly when the serological tests give doubtful results on individual animals.</abstract><cop>United States</cop><pub>Mary Ann Liebert, Inc., publishers</pub><pmid>32077807</pmid><doi>10.1089/vbz.2019.2537</doi><tpages>6</tpages></addata></record> |
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| ispartof | Vector borne and zoonotic diseases (Larchmont, N.Y.), 2020-06, Vol.20 (6), p.412-417 |
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| subjects | Animals Bayesian analysis Brucellosis Brucellosis - diagnosis Brucellosis - microbiology Brucellosis - veterinary Camelidae Camels Camelus - microbiology Camelus dromedarius Diagnosis Enzyme-linked immunosorbent assay Enzyme-Linked Immunosorbent Assay - veterinary Geographical locations Original Articles Rose Bengal Sensitivity and Specificity Serologic Tests - veterinary Serological tests Skin tests Skin Tests - methods Skin Tests - veterinary |
| title | Preliminary Comparative Assessment of Brucellergene Skin Test for Diagnosis of Brucellosis in Dromedary Camels (Camelus dromedarius) |
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