A bioconjugated MoS2 based nanoplatform with increased binding efficiency to cancer cells
We evaluate the application of surfactant-free liquid-phase exfoliated MoS2 nanosheets as a nanoplatform for a cancer detection and treatment system equipped with an antibody–antigen based recognition element. Employing antigen–antibody binding, we increased the probability of the endocytosis of MoS...
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| Veröffentlicht in: | Biomaterials science 2020-04, Vol.8 (7), p.1973-1980 |
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| container_end_page | 1980 |
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| container_issue | 7 |
| container_start_page | 1973 |
| container_title | Biomaterials science |
| container_volume | 8 |
| creator | Kálosi, Anna Labudová, Martina Annušová, Adriana Benkovičová, Monika Bodík, Michal Kollár, Jozef Kotlár, Mário Kasak, Peter Jergel, Matej Pastoreková, Silvia Siffalovic, Peter Majkova, Eva |
| description | We evaluate the application of surfactant-free liquid-phase exfoliated MoS2 nanosheets as a nanoplatform for a cancer detection and treatment system equipped with an antibody–antigen based recognition element. Employing antigen–antibody binding, we increased the probability of the endocytosis of MoS2 nanosheets into CAIX expressing cells by 30%. The nanosheets are functionalized with a specific antibody M75, which forms an antigen–antibody complex with CAIX. The bioconjugation of MoS2 nanosheets involves biocompatible components with low cytotoxicity, verified in the tested cell lines by fluorescence-based cell viability assay. The cellular internalization is quantified by flow cytometry, while the internalization is confirmed by label-free confocal Raman imaging. Raman measurements show increased lysosomal activity in the proximity of the internalized nanoplatforms. |
| doi_str_mv | 10.1039/c9bm01975h |
| format | Article |
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Employing antigen–antibody binding, we increased the probability of the endocytosis of MoS2 nanosheets into CAIX expressing cells by 30%. The nanosheets are functionalized with a specific antibody M75, which forms an antigen–antibody complex with CAIX. The bioconjugation of MoS2 nanosheets involves biocompatible components with low cytotoxicity, verified in the tested cell lines by fluorescence-based cell viability assay. The cellular internalization is quantified by flow cytometry, while the internalization is confirmed by label-free confocal Raman imaging. Raman measurements show increased lysosomal activity in the proximity of the internalized nanoplatforms.</description><subject>Antibodies</subject><subject>Antigens</subject><subject>Binding</subject><subject>Biocompatibility</subject><subject>Cancer</subject><subject>Exfoliation</subject><subject>Flow cytometry</subject><subject>Fluorescence</subject><subject>Liquid phases</subject><subject>Molybdenum disulfide</subject><subject>Nanostructure</subject><subject>Toxicity testing</subject><issn>2047-4830</issn><issn>2047-4849</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNpdj01LAzEQhoMoWGov_oKAFy-rk83Hbo6lqBUqHtSDp5LNTtqUbVI3u4j_3viBB-cyL8zD8LyEnDO4YsD1tdXNHpiu5PaITEoQVSFqoY__ModTMktpB3mqSoNiE_I6p42PNobduDEDtvQhPpW0MSnHYEI8dGZwsd_Tdz9sqQ-2x-9b40Prw4aic956DPaDDpFaEyz21GLXpTNy4kyXcPa7p-Tl9uZ5sSxWj3f3i_mqOJRMDQUvwbVOOTQCLFNMQ65heSmsbKDlKMpW1-Ca3FBw5pSUimNWRycMWKz5lFz-_D308W3ENKz3Pn0ZmIBxTOuSS6VASuAZvfiH7uLYh2yXqVpIqGrN-SdhgWIH</recordid><startdate>20200407</startdate><enddate>20200407</enddate><creator>Kálosi, Anna</creator><creator>Labudová, Martina</creator><creator>Annušová, Adriana</creator><creator>Benkovičová, Monika</creator><creator>Bodík, Michal</creator><creator>Kollár, Jozef</creator><creator>Kotlár, Mário</creator><creator>Kasak, Peter</creator><creator>Jergel, Matej</creator><creator>Pastoreková, Silvia</creator><creator>Siffalovic, Peter</creator><creator>Majkova, Eva</creator><general>Royal Society of Chemistry</general><scope>7SR</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>7X8</scope></search><sort><creationdate>20200407</creationdate><title>A bioconjugated MoS2 based nanoplatform with increased binding efficiency to cancer cells</title><author>Kálosi, Anna ; Labudová, Martina ; Annušová, Adriana ; Benkovičová, Monika ; Bodík, Michal ; Kollár, Jozef ; Kotlár, Mário ; Kasak, Peter ; Jergel, Matej ; Pastoreková, Silvia ; Siffalovic, Peter ; Majkova, Eva</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p216t-320fdf6fea40c16190c9bc324c5b0d3e42d980fb103431f65563e061ef4a0ce83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Antibodies</topic><topic>Antigens</topic><topic>Binding</topic><topic>Biocompatibility</topic><topic>Cancer</topic><topic>Exfoliation</topic><topic>Flow cytometry</topic><topic>Fluorescence</topic><topic>Liquid phases</topic><topic>Molybdenum disulfide</topic><topic>Nanostructure</topic><topic>Toxicity testing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kálosi, Anna</creatorcontrib><creatorcontrib>Labudová, Martina</creatorcontrib><creatorcontrib>Annušová, Adriana</creatorcontrib><creatorcontrib>Benkovičová, Monika</creatorcontrib><creatorcontrib>Bodík, Michal</creatorcontrib><creatorcontrib>Kollár, Jozef</creatorcontrib><creatorcontrib>Kotlár, Mário</creatorcontrib><creatorcontrib>Kasak, Peter</creatorcontrib><creatorcontrib>Jergel, Matej</creatorcontrib><creatorcontrib>Pastoreková, Silvia</creatorcontrib><creatorcontrib>Siffalovic, Peter</creatorcontrib><creatorcontrib>Majkova, Eva</creatorcontrib><collection>Engineered Materials Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>MEDLINE - Academic</collection><jtitle>Biomaterials science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kálosi, Anna</au><au>Labudová, Martina</au><au>Annušová, Adriana</au><au>Benkovičová, Monika</au><au>Bodík, Michal</au><au>Kollár, Jozef</au><au>Kotlár, Mário</au><au>Kasak, Peter</au><au>Jergel, Matej</au><au>Pastoreková, Silvia</au><au>Siffalovic, Peter</au><au>Majkova, Eva</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A bioconjugated MoS2 based nanoplatform with increased binding efficiency to cancer cells</atitle><jtitle>Biomaterials science</jtitle><date>2020-04-07</date><risdate>2020</risdate><volume>8</volume><issue>7</issue><spage>1973</spage><epage>1980</epage><pages>1973-1980</pages><issn>2047-4830</issn><eissn>2047-4849</eissn><abstract>We evaluate the application of surfactant-free liquid-phase exfoliated MoS2 nanosheets as a nanoplatform for a cancer detection and treatment system equipped with an antibody–antigen based recognition element. Employing antigen–antibody binding, we increased the probability of the endocytosis of MoS2 nanosheets into CAIX expressing cells by 30%. The nanosheets are functionalized with a specific antibody M75, which forms an antigen–antibody complex with CAIX. The bioconjugation of MoS2 nanosheets involves biocompatible components with low cytotoxicity, verified in the tested cell lines by fluorescence-based cell viability assay. The cellular internalization is quantified by flow cytometry, while the internalization is confirmed by label-free confocal Raman imaging. Raman measurements show increased lysosomal activity in the proximity of the internalized nanoplatforms.</abstract><cop>Cambridge</cop><pub>Royal Society of Chemistry</pub><doi>10.1039/c9bm01975h</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2047-4830 |
| ispartof | Biomaterials science, 2020-04, Vol.8 (7), p.1973-1980 |
| issn | 2047-4830 2047-4849 |
| language | eng |
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| source | Royal Society Of Chemistry Journals |
| subjects | Antibodies Antigens Binding Biocompatibility Cancer Exfoliation Flow cytometry Fluorescence Liquid phases Molybdenum disulfide Nanostructure Toxicity testing |
| title | A bioconjugated MoS2 based nanoplatform with increased binding efficiency to cancer cells |
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