Unravelling kinetic and microbial responses of enriched nitrifying sludge under long-term exposure of cephalexin and sulfadiazine
Wastewater treatment plants (WWTPs) have been identified as one of the reservoirs of antibiotics. Although nitrifying bacteria have been reported to be capable of degrading various antibiotics, there are very few studies investigating long-term effects of antibiotics on kinetic and microbial respons...
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| Veröffentlicht in: | Water research (Oxford) 2020-04, Vol.173, p.115592-115592, Article 115592 |
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| creator | Wang, Bingzheng Ni, Bing-Jie Yuan, Zhiguo Guo, Jianhua |
| description | Wastewater treatment plants (WWTPs) have been identified as one of the reservoirs of antibiotics. Although nitrifying bacteria have been reported to be capable of degrading various antibiotics, there are very few studies investigating long-term effects of antibiotics on kinetic and microbial responses of nitrifying bacteria. In this study, cephalexin (CFX) and sulfadiazine (SDZ) were selected to assess chronic impacts on nitrifying sludge with stepwise increasing concentrations in two independent bioreactors. The results showed that CFX and SDZ at an initial concentration of 100 μg/L could be efficiently removed by enriched nitrifying sludge, as evidenced by removal efficiencies of more than 88% and 85%, respectively. Ammonia-oxidizing bacteria (AOB) made a major contribution to the biodegradation of CFX and SDZ via cometabolism, compared to limited contributions from heterotrophic bacteria and nitrite-oxidizing bacteria. Chronic exposure to CFX (≥30 μg/L) could stimulate ammonium oxidation activity in terms of a significant enhancement of ammonium oxidation rate (p |
| doi_str_mv | 10.1016/j.watres.2020.115592 |
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[Display omitted]
•Cephalexin (CFX) and sulfadiazine (SDZ) could be efficiently removed by enriched nitrifying sludge.•AOB made major a contribution to the biodegradation of CFX and SDZ via cometabolism.•CFX could stimulate ammonium oxidation rate (AOR), while SDZ would decrease AOR.•Chronic exposure to CFX and SDZ caused decrease in the abundance of AOB.•The amoA gene was up-regulated to maintain stable removal of ammonium and antibiotics.</description><identifier>ISSN: 0043-1354</identifier><identifier>EISSN: 1879-2448</identifier><identifier>DOI: 10.1016/j.watres.2020.115592</identifier><identifier>PMID: 32062227</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Ammonia ; Ammonia-oxidizing bacteria (AOB) ; amoA mRNA ; Antibiotic biodegradation ; Bioreactors ; Cephalexin ; Cometabolism ; Nitrification ; Oxidation-Reduction ; RNA, Ribosomal, 16S ; Sewage ; Sulfadiazine</subject><ispartof>Water research (Oxford), 2020-04, Vol.173, p.115592-115592, Article 115592</ispartof><rights>2020 Elsevier Ltd</rights><rights>Copyright © 2020 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c362t-79b2f8d879973b59d2af508550bb1b87c619d1cc1900ddba6a857d2ac5e0bdc73</citedby><cites>FETCH-LOGICAL-c362t-79b2f8d879973b59d2af508550bb1b87c619d1cc1900ddba6a857d2ac5e0bdc73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.watres.2020.115592$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32062227$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Bingzheng</creatorcontrib><creatorcontrib>Ni, Bing-Jie</creatorcontrib><creatorcontrib>Yuan, Zhiguo</creatorcontrib><creatorcontrib>Guo, Jianhua</creatorcontrib><title>Unravelling kinetic and microbial responses of enriched nitrifying sludge under long-term exposure of cephalexin and sulfadiazine</title><title>Water research (Oxford)</title><addtitle>Water Res</addtitle><description>Wastewater treatment plants (WWTPs) have been identified as one of the reservoirs of antibiotics. Although nitrifying bacteria have been reported to be capable of degrading various antibiotics, there are very few studies investigating long-term effects of antibiotics on kinetic and microbial responses of nitrifying bacteria. In this study, cephalexin (CFX) and sulfadiazine (SDZ) were selected to assess chronic impacts on nitrifying sludge with stepwise increasing concentrations in two independent bioreactors. The results showed that CFX and SDZ at an initial concentration of 100 μg/L could be efficiently removed by enriched nitrifying sludge, as evidenced by removal efficiencies of more than 88% and 85%, respectively. Ammonia-oxidizing bacteria (AOB) made a major contribution to the biodegradation of CFX and SDZ via cometabolism, compared to limited contributions from heterotrophic bacteria and nitrite-oxidizing bacteria. Chronic exposure to CFX (≥30 μg/L) could stimulate ammonium oxidation activity in terms of a significant enhancement of ammonium oxidation rate (p < 0.01). In contrast, the ammonium oxidation activity was inhibited due to exposure to 30 μg/L SDZ (p < 0.01), then it recovered after long-term adaption under exposure to 50 and 100 μg/L SDZ. In addition, 16S rRNA gene amplicon sequencing revealed that the relative abundance of AOB decreased distinctly from 23.8% to 28.8% in the control phase (without CFX or SDZ) to 14.2% and 10.8% under exposure to 100 μg/L CFX and SDZ, respectively. However, the expression level of amoA gene was up-regulated to overcome this adverse impact and maintain a stable and efficient removal of both ammonium and antibiotics. The findings in this study shed a light on chronic effects of antibiotic exposure on kinetic and microbial responses of enriched nitrifying sludge in WWTPs.
[Display omitted]
•Cephalexin (CFX) and sulfadiazine (SDZ) could be efficiently removed by enriched nitrifying sludge.•AOB made major a contribution to the biodegradation of CFX and SDZ via cometabolism.•CFX could stimulate ammonium oxidation rate (AOR), while SDZ would decrease AOR.•Chronic exposure to CFX and SDZ caused decrease in the abundance of AOB.•The amoA gene was up-regulated to maintain stable removal of ammonium and antibiotics.</description><subject>Ammonia</subject><subject>Ammonia-oxidizing bacteria (AOB)</subject><subject>amoA mRNA</subject><subject>Antibiotic biodegradation</subject><subject>Bioreactors</subject><subject>Cephalexin</subject><subject>Cometabolism</subject><subject>Nitrification</subject><subject>Oxidation-Reduction</subject><subject>RNA, Ribosomal, 16S</subject><subject>Sewage</subject><subject>Sulfadiazine</subject><issn>0043-1354</issn><issn>1879-2448</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kDtv2zAUhYkiReO4_QdFwDGLXJISJXEpEBh5AQG61DPBx5VNR6JUUnIeW_956crN2IkA8Z17cD6EvlKyooSW3_arZzUGiCtGWPqinAv2AS1oXYmMFUV9hhaEFHlGc16co4sY94QQxnLxCZ3njJSMsWqBfm98UAdoW-e3-Ml5GJ3BylvcORN67VSLU8fQ-wgR9w0GH5zZgcXejcE1r8dYbCe7BTx5CwG3vd9mI4QOw8vQxynAMWZg2KkWXpz_ezxObaOsU2-p8DP62Kg2wpfTu0Sb25uf6_vs8cfdw_r6MTN5ycasEpo1tU3rRJVrLixTDSc150RrquvKlFRYagwVhFirValqXiXIcCDamipfoqv57hD6XxPEUXYumrRceeinKFnOSy6KgomEFjOaFMQYoJFDcJ0Kr5ISeZQv93KWL4_y5Sw_xS5PDZPuwL6H_tlOwPcZgLTz4CDIaBx4A9YFMKO0vft_wx9bb5sJ</recordid><startdate>20200415</startdate><enddate>20200415</enddate><creator>Wang, Bingzheng</creator><creator>Ni, Bing-Jie</creator><creator>Yuan, Zhiguo</creator><creator>Guo, Jianhua</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20200415</creationdate><title>Unravelling kinetic and microbial responses of enriched nitrifying sludge under long-term exposure of cephalexin and sulfadiazine</title><author>Wang, Bingzheng ; Ni, Bing-Jie ; Yuan, Zhiguo ; Guo, Jianhua</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c362t-79b2f8d879973b59d2af508550bb1b87c619d1cc1900ddba6a857d2ac5e0bdc73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Ammonia</topic><topic>Ammonia-oxidizing bacteria (AOB)</topic><topic>amoA mRNA</topic><topic>Antibiotic biodegradation</topic><topic>Bioreactors</topic><topic>Cephalexin</topic><topic>Cometabolism</topic><topic>Nitrification</topic><topic>Oxidation-Reduction</topic><topic>RNA, Ribosomal, 16S</topic><topic>Sewage</topic><topic>Sulfadiazine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Bingzheng</creatorcontrib><creatorcontrib>Ni, Bing-Jie</creatorcontrib><creatorcontrib>Yuan, Zhiguo</creatorcontrib><creatorcontrib>Guo, Jianhua</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Water research (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Bingzheng</au><au>Ni, Bing-Jie</au><au>Yuan, Zhiguo</au><au>Guo, Jianhua</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Unravelling kinetic and microbial responses of enriched nitrifying sludge under long-term exposure of cephalexin and sulfadiazine</atitle><jtitle>Water research (Oxford)</jtitle><addtitle>Water Res</addtitle><date>2020-04-15</date><risdate>2020</risdate><volume>173</volume><spage>115592</spage><epage>115592</epage><pages>115592-115592</pages><artnum>115592</artnum><issn>0043-1354</issn><eissn>1879-2448</eissn><abstract>Wastewater treatment plants (WWTPs) have been identified as one of the reservoirs of antibiotics. Although nitrifying bacteria have been reported to be capable of degrading various antibiotics, there are very few studies investigating long-term effects of antibiotics on kinetic and microbial responses of nitrifying bacteria. In this study, cephalexin (CFX) and sulfadiazine (SDZ) were selected to assess chronic impacts on nitrifying sludge with stepwise increasing concentrations in two independent bioreactors. The results showed that CFX and SDZ at an initial concentration of 100 μg/L could be efficiently removed by enriched nitrifying sludge, as evidenced by removal efficiencies of more than 88% and 85%, respectively. Ammonia-oxidizing bacteria (AOB) made a major contribution to the biodegradation of CFX and SDZ via cometabolism, compared to limited contributions from heterotrophic bacteria and nitrite-oxidizing bacteria. Chronic exposure to CFX (≥30 μg/L) could stimulate ammonium oxidation activity in terms of a significant enhancement of ammonium oxidation rate (p < 0.01). In contrast, the ammonium oxidation activity was inhibited due to exposure to 30 μg/L SDZ (p < 0.01), then it recovered after long-term adaption under exposure to 50 and 100 μg/L SDZ. In addition, 16S rRNA gene amplicon sequencing revealed that the relative abundance of AOB decreased distinctly from 23.8% to 28.8% in the control phase (without CFX or SDZ) to 14.2% and 10.8% under exposure to 100 μg/L CFX and SDZ, respectively. However, the expression level of amoA gene was up-regulated to overcome this adverse impact and maintain a stable and efficient removal of both ammonium and antibiotics. The findings in this study shed a light on chronic effects of antibiotic exposure on kinetic and microbial responses of enriched nitrifying sludge in WWTPs.
[Display omitted]
•Cephalexin (CFX) and sulfadiazine (SDZ) could be efficiently removed by enriched nitrifying sludge.•AOB made major a contribution to the biodegradation of CFX and SDZ via cometabolism.•CFX could stimulate ammonium oxidation rate (AOR), while SDZ would decrease AOR.•Chronic exposure to CFX and SDZ caused decrease in the abundance of AOB.•The amoA gene was up-regulated to maintain stable removal of ammonium and antibiotics.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>32062227</pmid><doi>10.1016/j.watres.2020.115592</doi><tpages>1</tpages></addata></record> |
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| subjects | Ammonia Ammonia-oxidizing bacteria (AOB) amoA mRNA Antibiotic biodegradation Bioreactors Cephalexin Cometabolism Nitrification Oxidation-Reduction RNA, Ribosomal, 16S Sewage Sulfadiazine |
| title | Unravelling kinetic and microbial responses of enriched nitrifying sludge under long-term exposure of cephalexin and sulfadiazine |
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