A Simple and Effective Technique for Production of Pycnidia and Pycnidiospores by Macrophomina phaseolina
Production of pycnidia and pycnidiospores by is not often seen in vitro. The objective of this study is to develop a simple and effective technique to obtain pycnidiospores of isolates in vitro and to evaluate germination rates and pathogenicity of pycnidiospores. We found isolates can produce pycni...
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| Veröffentlicht in: | Plant disease 2020-04, Vol.104 (4), p.1183-1187 |
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| creator | Zhao, Xinbei Ni, Yunxia Liu, Xintao Zhao, Hui Wang, Jing Chen, Yung-Chun Chen, Weidong Liu, Hongyan |
| description | Production of pycnidia and pycnidiospores by
is not often seen in vitro. The objective of this study is to develop a simple and effective technique to obtain pycnidiospores of
isolates in vitro and to evaluate germination rates and pathogenicity of pycnidiospores. We found
isolates can produce pycnidia on oatmeal agar (OMA) under ultraviolet light with 365 nm wavelength (UV). For evaluating the effect of OMA and UV on growth of
, combinations of two agar media and three lighting conditions were tested. The results confirm that all six
isolates produced pycnidia only on OMA under UV. The pycnidiospores produced on OMA under UV had germination rates higher than 90%. In pathogenicity tests, inoculation with the pycnidiospores showed symptoms later than inoculation with hypha-colonized toothpicks. Significant differences in the pathogenicity is detected between isolates Mp2014003 and Mp2014024 when inoculation is done with the pycnidiospores (
< 0.001), but not when hypha-colonized toothpicks are used as inoculum (
= 0.091). This study provides a new method for obtaining pycnidiospores of
for future investigations. |
| doi_str_mv | 10.1094/PDIS-08-19-1795-RE |
| format | Article |
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is not often seen in vitro. The objective of this study is to develop a simple and effective technique to obtain pycnidiospores of
isolates in vitro and to evaluate germination rates and pathogenicity of pycnidiospores. We found
isolates can produce pycnidia on oatmeal agar (OMA) under ultraviolet light with 365 nm wavelength (UV). For evaluating the effect of OMA and UV on growth of
, combinations of two agar media and three lighting conditions were tested. The results confirm that all six
isolates produced pycnidia only on OMA under UV. The pycnidiospores produced on OMA under UV had germination rates higher than 90%. In pathogenicity tests, inoculation with the pycnidiospores showed symptoms later than inoculation with hypha-colonized toothpicks. Significant differences in the pathogenicity is detected between isolates Mp2014003 and Mp2014024 when inoculation is done with the pycnidiospores (
< 0.001), but not when hypha-colonized toothpicks are used as inoculum (
= 0.091). This study provides a new method for obtaining pycnidiospores of
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is not often seen in vitro. The objective of this study is to develop a simple and effective technique to obtain pycnidiospores of
isolates in vitro and to evaluate germination rates and pathogenicity of pycnidiospores. We found
isolates can produce pycnidia on oatmeal agar (OMA) under ultraviolet light with 365 nm wavelength (UV). For evaluating the effect of OMA and UV on growth of
, combinations of two agar media and three lighting conditions were tested. The results confirm that all six
isolates produced pycnidia only on OMA under UV. The pycnidiospores produced on OMA under UV had germination rates higher than 90%. In pathogenicity tests, inoculation with the pycnidiospores showed symptoms later than inoculation with hypha-colonized toothpicks. Significant differences in the pathogenicity is detected between isolates Mp2014003 and Mp2014024 when inoculation is done with the pycnidiospores (
< 0.001), but not when hypha-colonized toothpicks are used as inoculum (
= 0.091). This study provides a new method for obtaining pycnidiospores of
for future investigations.</description><subject>Agar</subject><subject>Ascomycota</subject><subject>Culture Media</subject><subject>Plant Diseases</subject><issn>0191-2917</issn><issn>1943-7692</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kM1OwzAQhC0EoqXwAhyQj1wM_kmc-FiVApWKqNpythxnrRolcYhbpL49KS2cdlc7Mxp9CN0y-sCoSh4XT7MVoTlhirBMpWQ5PUNDphJBMqn4ORpSphjhimUDdBXjJ6U0SWR-iQaCU5mmUg6RH-OVr9sKsGlKPHUO7NZ_A16D3TT-awfYhQ4vulDu-kdocHB4sbeNL735tZyOENvQQcTFHr8Z24V2E2rfGNxuTIRQ9es1unCminBzmiP08TxdT17J_P1lNhnPiRVJtiV5kadcGpO4wnCTKAtFIrlzTlkDtsyYsq4UBXMgc0mNYGB4YW0BIhMm41SM0P0xt-1C3z9ude2jhaoyDYRd1FykMs1zxWQv5UdpXzjGDpxuO1-bbq8Z1QfE-oBY01wzpQ-I9XLam-5O-buihvLf8sdU_ACtlHp1</recordid><startdate>20200401</startdate><enddate>20200401</enddate><creator>Zhao, Xinbei</creator><creator>Ni, Yunxia</creator><creator>Liu, Xintao</creator><creator>Zhao, Hui</creator><creator>Wang, Jing</creator><creator>Chen, Yung-Chun</creator><creator>Chen, Weidong</creator><creator>Liu, Hongyan</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-0284-8761</orcidid><orcidid>https://orcid.org/0000-0001-5421-984X</orcidid></search><sort><creationdate>20200401</creationdate><title>A Simple and Effective Technique for Production of Pycnidia and Pycnidiospores by Macrophomina phaseolina</title><author>Zhao, Xinbei ; Ni, Yunxia ; Liu, Xintao ; Zhao, Hui ; Wang, Jing ; Chen, Yung-Chun ; Chen, Weidong ; Liu, Hongyan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c347t-8b8526aa4fba2a49ceb462fff9caecd719cfd3b1fe6860a31ea2bccbe373a7203</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Agar</topic><topic>Ascomycota</topic><topic>Culture Media</topic><topic>Plant Diseases</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhao, Xinbei</creatorcontrib><creatorcontrib>Ni, Yunxia</creatorcontrib><creatorcontrib>Liu, Xintao</creatorcontrib><creatorcontrib>Zhao, Hui</creatorcontrib><creatorcontrib>Wang, Jing</creatorcontrib><creatorcontrib>Chen, Yung-Chun</creatorcontrib><creatorcontrib>Chen, Weidong</creatorcontrib><creatorcontrib>Liu, Hongyan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Plant disease</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhao, Xinbei</au><au>Ni, Yunxia</au><au>Liu, Xintao</au><au>Zhao, Hui</au><au>Wang, Jing</au><au>Chen, Yung-Chun</au><au>Chen, Weidong</au><au>Liu, Hongyan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Simple and Effective Technique for Production of Pycnidia and Pycnidiospores by Macrophomina phaseolina</atitle><jtitle>Plant disease</jtitle><addtitle>Plant Dis</addtitle><date>2020-04-01</date><risdate>2020</risdate><volume>104</volume><issue>4</issue><spage>1183</spage><epage>1187</epage><pages>1183-1187</pages><issn>0191-2917</issn><eissn>1943-7692</eissn><abstract>Production of pycnidia and pycnidiospores by
is not often seen in vitro. The objective of this study is to develop a simple and effective technique to obtain pycnidiospores of
isolates in vitro and to evaluate germination rates and pathogenicity of pycnidiospores. We found
isolates can produce pycnidia on oatmeal agar (OMA) under ultraviolet light with 365 nm wavelength (UV). For evaluating the effect of OMA and UV on growth of
, combinations of two agar media and three lighting conditions were tested. The results confirm that all six
isolates produced pycnidia only on OMA under UV. The pycnidiospores produced on OMA under UV had germination rates higher than 90%. In pathogenicity tests, inoculation with the pycnidiospores showed symptoms later than inoculation with hypha-colonized toothpicks. Significant differences in the pathogenicity is detected between isolates Mp2014003 and Mp2014024 when inoculation is done with the pycnidiospores (
< 0.001), but not when hypha-colonized toothpicks are used as inoculum (
= 0.091). This study provides a new method for obtaining pycnidiospores of
for future investigations.</abstract><cop>United States</cop><pmid>32065566</pmid><doi>10.1094/PDIS-08-19-1795-RE</doi><tpages>5</tpages><orcidid>https://orcid.org/0000-0003-0284-8761</orcidid><orcidid>https://orcid.org/0000-0001-5421-984X</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Plant disease, 2020-04, Vol.104 (4), p.1183-1187 |
| issn | 0191-2917 1943-7692 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2356588916 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection; American Phytopathological Society Journal Back Issues |
| subjects | Agar Ascomycota Culture Media Plant Diseases |
| title | A Simple and Effective Technique for Production of Pycnidia and Pycnidiospores by Macrophomina phaseolina |
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