Characterization and anti-diabetic effects of the oligosaccharide fraction isolated from Rosa canina in STZ-Induced diabetic rats
Diabetes mellitus is the most common metabolic disorder characterized by chronic hyperglycemia. There has been a surge of research studies aiming to use natural products in the management of diabetes. The objective of this study was to isolate and characterize the structure and anti-diabetic mechani...
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| Veröffentlicht in: | Carbohydrate research 2020-03, Vol.489, p.107927-107927, Article 107927 |
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| creator | Rahimi, Mehrali Sajadimajd, Soraya Mahdian, Zahra Hemmati, Marayam Malekkhatabi, Peyman Bahrami, Gholamreza Mohammadi, Bahareh Miraghaee, Shahram Hatami, Razieh Mansouri, Kamran Moahammadi Motlagh, Hamid Reza Keshavarzi, Samira Derakhshankhah, Hossein |
| description | Diabetes mellitus is the most common metabolic disorder characterized by chronic hyperglycemia. There has been a surge of research studies aiming to use natural products in the management of diabetes. The objective of this study was to isolate and characterize the structure and anti-diabetic mechanisms of the main ingredient from Rosa canina. The oligosaccharide was isolated from Rosa canina fruits and characterized by a combination of FTIR, NMR and Mass spectrometry. Wistar rats were divided into negative control, diabetic (type 2), isolated oligosaccharide (IO)-treated diabetic and positive diabetic controls. Oral glucose tolerance, gluconeogenesis and α-glucosidase inhibitory tests as well as immunohistochemistry and quantitative real time-PCR were performed to elucidate the molecular anti-diabetic mechanisms of IO. Structural analyses confirmed the oligosaccharide structure of isolated fraction. Gluconeogenesis and α-glucosidase activity were inhibited by IO in diabetic rats. The oral glucose tolerance test was improved significantly in the group treated with the IO (P |
| doi_str_mv | 10.1016/j.carres.2020.107927 |
| format | Article |
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•Oligosaccharide fraction was isolated from Rosa canina, purified and characterized via HPLC, IR, NMR and MS analyses.•Isolated oligosaccharide from R. canina normalized blood glucose level in STZ-induced diabetic rats.•Isolated oligosaccharide increased the expression of Insulin, Ngn3 and Nkx6.1.•Gluconeogenesis and α-glucosidase activity fell into the normal range in oligosaccharide-treated diabetic animals.</description><identifier>ISSN: 0008-6215</identifier><identifier>EISSN: 1873-426X</identifier><identifier>DOI: 10.1016/j.carres.2020.107927</identifier><identifier>PMID: 32062396</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Diabetes ; Oligosaccharide ; Regeneration ; Rosa canina</subject><ispartof>Carbohydrate research, 2020-03, Vol.489, p.107927-107927, Article 107927</ispartof><rights>2020</rights><rights>Copyright © 2020. Published by Elsevier Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c362t-e50542caf78fe8f802bc456ddc05ad05a6fb04e8caebd79d0e86f24bb57b38df3</citedby><cites>FETCH-LOGICAL-c362t-e50542caf78fe8f802bc456ddc05ad05a6fb04e8caebd79d0e86f24bb57b38df3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.carres.2020.107927$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32062396$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rahimi, Mehrali</creatorcontrib><creatorcontrib>Sajadimajd, Soraya</creatorcontrib><creatorcontrib>Mahdian, Zahra</creatorcontrib><creatorcontrib>Hemmati, Marayam</creatorcontrib><creatorcontrib>Malekkhatabi, Peyman</creatorcontrib><creatorcontrib>Bahrami, Gholamreza</creatorcontrib><creatorcontrib>Mohammadi, Bahareh</creatorcontrib><creatorcontrib>Miraghaee, Shahram</creatorcontrib><creatorcontrib>Hatami, Razieh</creatorcontrib><creatorcontrib>Mansouri, Kamran</creatorcontrib><creatorcontrib>Moahammadi Motlagh, Hamid Reza</creatorcontrib><creatorcontrib>Keshavarzi, Samira</creatorcontrib><creatorcontrib>Derakhshankhah, Hossein</creatorcontrib><title>Characterization and anti-diabetic effects of the oligosaccharide fraction isolated from Rosa canina in STZ-Induced diabetic rats</title><title>Carbohydrate research</title><addtitle>Carbohydr Res</addtitle><description>Diabetes mellitus is the most common metabolic disorder characterized by chronic hyperglycemia. There has been a surge of research studies aiming to use natural products in the management of diabetes. The objective of this study was to isolate and characterize the structure and anti-diabetic mechanisms of the main ingredient from Rosa canina. The oligosaccharide was isolated from Rosa canina fruits and characterized by a combination of FTIR, NMR and Mass spectrometry. Wistar rats were divided into negative control, diabetic (type 2), isolated oligosaccharide (IO)-treated diabetic and positive diabetic controls. Oral glucose tolerance, gluconeogenesis and α-glucosidase inhibitory tests as well as immunohistochemistry and quantitative real time-PCR were performed to elucidate the molecular anti-diabetic mechanisms of IO. Structural analyses confirmed the oligosaccharide structure of isolated fraction. Gluconeogenesis and α-glucosidase activity were inhibited by IO in diabetic rats. The oral glucose tolerance test was improved significantly in the group treated with the IO (P < 0.05). Pancreatic β-cells and tissue pathological examination showed a significant improvement after the treatment period. In addition, the expression of Ngn3, Nkx6.1 and insulin increased in oligosaccharide-treated compared to untreated diabetic rats. Owing to the verified anti-diabetic effects and regenerative potential, isolated oligosaccharide could be considered as the promising drug in the management of diabetes.
[Display omitted]
•Oligosaccharide fraction was isolated from Rosa canina, purified and characterized via HPLC, IR, NMR and MS analyses.•Isolated oligosaccharide from R. canina normalized blood glucose level in STZ-induced diabetic rats.•Isolated oligosaccharide increased the expression of Insulin, Ngn3 and Nkx6.1.•Gluconeogenesis and α-glucosidase activity fell into the normal range in oligosaccharide-treated diabetic animals.</description><subject>Diabetes</subject><subject>Oligosaccharide</subject><subject>Regeneration</subject><subject>Rosa canina</subject><issn>0008-6215</issn><issn>1873-426X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp9kE1LJDEQhoO46PjxD0Ry9NJjOt1J91wEGfwYEBZ2XRAvoTqpaIaejiYZwb3tPzdNq8c9FCGV96kiDyEnJZuXrJTn67mGEDDOOeNjq1nwZofMyrapiprLh10yY4y1heSl2CcHMa7zlclG7pH9ijPJq4WckX_LZwigEwb3F5LzA4XB5EquMA46TE5TtBZ1itRbmp6R-t49-QhaZ9IZpHbkR9JF30NCkzt-Q3_lDNUwuAGoG-jv-8diNZitzu_fkwOkeER-WOgjHn-eh-TP9dX98ra4-3mzWl7eFbqSPBUomKi5Btu0FlvbMt7pWkhjNBNgcknbsRpbDdiZZmEYttLyuutE01WtsdUhOZvmvgT_usWY1MZFjX0PA_ptVLwSUjSyFGWO1lNUBx9jQKtegttAeFclU6N8tVaTfDXKV5P8jJ1-bth2GzTf0JftHLiYApj_-eYwqKgdDlmJC9mwMt79f8MHW2eaag</recordid><startdate>202003</startdate><enddate>202003</enddate><creator>Rahimi, Mehrali</creator><creator>Sajadimajd, Soraya</creator><creator>Mahdian, Zahra</creator><creator>Hemmati, Marayam</creator><creator>Malekkhatabi, Peyman</creator><creator>Bahrami, Gholamreza</creator><creator>Mohammadi, Bahareh</creator><creator>Miraghaee, Shahram</creator><creator>Hatami, Razieh</creator><creator>Mansouri, Kamran</creator><creator>Moahammadi Motlagh, Hamid Reza</creator><creator>Keshavarzi, Samira</creator><creator>Derakhshankhah, Hossein</creator><general>Elsevier Ltd</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>202003</creationdate><title>Characterization and anti-diabetic effects of the oligosaccharide fraction isolated from Rosa canina in STZ-Induced diabetic rats</title><author>Rahimi, Mehrali ; Sajadimajd, Soraya ; Mahdian, Zahra ; Hemmati, Marayam ; Malekkhatabi, Peyman ; Bahrami, Gholamreza ; Mohammadi, Bahareh ; Miraghaee, Shahram ; Hatami, Razieh ; Mansouri, Kamran ; Moahammadi Motlagh, Hamid Reza ; Keshavarzi, Samira ; Derakhshankhah, Hossein</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c362t-e50542caf78fe8f802bc456ddc05ad05a6fb04e8caebd79d0e86f24bb57b38df3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Diabetes</topic><topic>Oligosaccharide</topic><topic>Regeneration</topic><topic>Rosa canina</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rahimi, Mehrali</creatorcontrib><creatorcontrib>Sajadimajd, Soraya</creatorcontrib><creatorcontrib>Mahdian, Zahra</creatorcontrib><creatorcontrib>Hemmati, Marayam</creatorcontrib><creatorcontrib>Malekkhatabi, Peyman</creatorcontrib><creatorcontrib>Bahrami, Gholamreza</creatorcontrib><creatorcontrib>Mohammadi, Bahareh</creatorcontrib><creatorcontrib>Miraghaee, Shahram</creatorcontrib><creatorcontrib>Hatami, Razieh</creatorcontrib><creatorcontrib>Mansouri, Kamran</creatorcontrib><creatorcontrib>Moahammadi Motlagh, Hamid Reza</creatorcontrib><creatorcontrib>Keshavarzi, Samira</creatorcontrib><creatorcontrib>Derakhshankhah, Hossein</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Carbohydrate research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rahimi, Mehrali</au><au>Sajadimajd, Soraya</au><au>Mahdian, Zahra</au><au>Hemmati, Marayam</au><au>Malekkhatabi, Peyman</au><au>Bahrami, Gholamreza</au><au>Mohammadi, Bahareh</au><au>Miraghaee, Shahram</au><au>Hatami, Razieh</au><au>Mansouri, Kamran</au><au>Moahammadi Motlagh, Hamid Reza</au><au>Keshavarzi, Samira</au><au>Derakhshankhah, Hossein</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization and anti-diabetic effects of the oligosaccharide fraction isolated from Rosa canina in STZ-Induced diabetic rats</atitle><jtitle>Carbohydrate research</jtitle><addtitle>Carbohydr Res</addtitle><date>2020-03</date><risdate>2020</risdate><volume>489</volume><spage>107927</spage><epage>107927</epage><pages>107927-107927</pages><artnum>107927</artnum><issn>0008-6215</issn><eissn>1873-426X</eissn><abstract>Diabetes mellitus is the most common metabolic disorder characterized by chronic hyperglycemia. There has been a surge of research studies aiming to use natural products in the management of diabetes. The objective of this study was to isolate and characterize the structure and anti-diabetic mechanisms of the main ingredient from Rosa canina. The oligosaccharide was isolated from Rosa canina fruits and characterized by a combination of FTIR, NMR and Mass spectrometry. Wistar rats were divided into negative control, diabetic (type 2), isolated oligosaccharide (IO)-treated diabetic and positive diabetic controls. Oral glucose tolerance, gluconeogenesis and α-glucosidase inhibitory tests as well as immunohistochemistry and quantitative real time-PCR were performed to elucidate the molecular anti-diabetic mechanisms of IO. Structural analyses confirmed the oligosaccharide structure of isolated fraction. Gluconeogenesis and α-glucosidase activity were inhibited by IO in diabetic rats. The oral glucose tolerance test was improved significantly in the group treated with the IO (P < 0.05). Pancreatic β-cells and tissue pathological examination showed a significant improvement after the treatment period. In addition, the expression of Ngn3, Nkx6.1 and insulin increased in oligosaccharide-treated compared to untreated diabetic rats. Owing to the verified anti-diabetic effects and regenerative potential, isolated oligosaccharide could be considered as the promising drug in the management of diabetes.
[Display omitted]
•Oligosaccharide fraction was isolated from Rosa canina, purified and characterized via HPLC, IR, NMR and MS analyses.•Isolated oligosaccharide from R. canina normalized blood glucose level in STZ-induced diabetic rats.•Isolated oligosaccharide increased the expression of Insulin, Ngn3 and Nkx6.1.•Gluconeogenesis and α-glucosidase activity fell into the normal range in oligosaccharide-treated diabetic animals.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>32062396</pmid><doi>10.1016/j.carres.2020.107927</doi><tpages>1</tpages></addata></record> |
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| subjects | Diabetes Oligosaccharide Regeneration Rosa canina |
| title | Characterization and anti-diabetic effects of the oligosaccharide fraction isolated from Rosa canina in STZ-Induced diabetic rats |
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