Proteomics profiling of epithelium-derived exosomes from nasal polyps revealed signaling functions affecting cellular proliferation
Nasal polyps are a significantly associated pathology of chronic rhinosinusitis (CRS) whose mechanisms of pathogenesis are not fully elucidated, especially the interaction of the polyp with its environment that allows its growth on the nasal epithelial lining. Exosomes are nanovesicles that serve im...
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Veröffentlicht in: | Respiratory medicine 2020-02, Vol.162, p.105871-105871, Article 105871 |
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creator | Zhou, Min Tan, Kai Sen Guan, Wei-jie Jiang, Li-jie Deng, Jie Gao, Wen-xiang Lee, Yew Mun Xu, Zhao-feng Luo, Xin Liu, Chen Shi, Jian-bo Lai, Yinyan |
description | Nasal polyps are a significantly associated pathology of chronic rhinosinusitis (CRS) whose mechanisms of pathogenesis are not fully elucidated, especially the interaction of the polyp with its environment that allows its growth on the nasal epithelial lining. Exosomes are nanovesicles that serve important biological functions, including cell-to-cell signaling and communication.
Hence, we sought to explore the roles of the epithelial-derived exosomal proteome obtained from the human nasal epithelium in the modulation of CRS with nasal polyp (CRSwNP) pathogenesis.
We sampled exosomes from nasal lavage fluid and primary human nasal epithelial cells (hNECs) from healthy controls and patients with CRSwNP with and without coexisting asthma. The presence of exosomes was confirmed using a NanoSight assay, transmission electron microscopy and western blotting. The exosomal proteome was profiled with mass spectrometry. The Cell Counting Kit-8 was used to confirm the roles of exosomes in mediating cellular proliferation.
The hNEC-derived exosomes from diseased epithelium contained differentially expressed proteins that were mainly involved in epithelial remodeling via pathways such as p53. An in vitro study further demonstrated that epithelial-derived exosomes from patients with CRSwNP (with and without coexisting asthma) significantly reduced the rate of proliferation of control hNECs at an effective concentration of ≥10 μg/ml.
Exosomes secreted by hNECs from patients with CRSwNP, regardless of their coexistence with asthma, are laden with proteins that influence cell proliferation pathways, potentially leading to remodeling of the sinonasal mucosa.
Diseased (CRSwNP with and without coexisting asthma) human nasal epithelial cells (hNECs) were found to communicate with the environment via exosomes. Both healthy and exosomal protein signatures include immune response cytokines. Diseased epithelium-derived exosomes secreted increased cell cycle signatures involved in such as p53 pathway that might contribute to the inhibition of healthy epithelial proliferation, potentially as a mechanism of remodeling. [Display omitted] |
doi_str_mv | 10.1016/j.rmed.2020.105871 |
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Hence, we sought to explore the roles of the epithelial-derived exosomal proteome obtained from the human nasal epithelium in the modulation of CRS with nasal polyp (CRSwNP) pathogenesis.
We sampled exosomes from nasal lavage fluid and primary human nasal epithelial cells (hNECs) from healthy controls and patients with CRSwNP with and without coexisting asthma. The presence of exosomes was confirmed using a NanoSight assay, transmission electron microscopy and western blotting. The exosomal proteome was profiled with mass spectrometry. The Cell Counting Kit-8 was used to confirm the roles of exosomes in mediating cellular proliferation.
The hNEC-derived exosomes from diseased epithelium contained differentially expressed proteins that were mainly involved in epithelial remodeling via pathways such as p53. An in vitro study further demonstrated that epithelial-derived exosomes from patients with CRSwNP (with and without coexisting asthma) significantly reduced the rate of proliferation of control hNECs at an effective concentration of ≥10 μg/ml.
Exosomes secreted by hNECs from patients with CRSwNP, regardless of their coexistence with asthma, are laden with proteins that influence cell proliferation pathways, potentially leading to remodeling of the sinonasal mucosa.
Diseased (CRSwNP with and without coexisting asthma) human nasal epithelial cells (hNECs) were found to communicate with the environment via exosomes. Both healthy and exosomal protein signatures include immune response cytokines. Diseased epithelium-derived exosomes secreted increased cell cycle signatures involved in such as p53 pathway that might contribute to the inhibition of healthy epithelial proliferation, potentially as a mechanism of remodeling. [Display omitted]</description><identifier>ISSN: 0954-6111</identifier><identifier>EISSN: 1532-3064</identifier><identifier>DOI: 10.1016/j.rmed.2020.105871</identifier><identifier>PMID: 32056672</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Antibiotics ; Asthma ; Cell proliferation ; Chronic rhinosinusitis ; Epithelial cells ; Epithelium ; Exosomes ; Fluorides ; Mass spectrometry ; Mass spectroscopy ; Medical diagnosis ; Membranes ; Mucosa ; Nasal polyps ; Nonsteroidal anti-inflammatory drugs ; p53 Protein ; Patients ; Polyps ; Proliferation ; Proteins ; Proteomes ; Proteomics ; Rhinitis ; Rhinosinusitis ; Scientific imaging ; Signal transduction ; Sinusitis ; Transmission electron microscopy ; Western blotting</subject><ispartof>Respiratory medicine, 2020-02, Vol.162, p.105871-105871, Article 105871</ispartof><rights>2020 Elsevier Ltd</rights><rights>Copyright © 2020 Elsevier Ltd. All rights reserved.</rights><rights>2020. Elsevier Ltd</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c494t-f4567348088f938cb1bc89c1bc8e489171cbfc09e04ae68374b5e2e367d56e0e3</citedby><cites>FETCH-LOGICAL-c494t-f4567348088f938cb1bc89c1bc8e489171cbfc09e04ae68374b5e2e367d56e0e3</cites><orcidid>0000-0003-4553-4383 ; 0000-0003-2193-7612</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.rmed.2020.105871$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32056672$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhou, Min</creatorcontrib><creatorcontrib>Tan, Kai Sen</creatorcontrib><creatorcontrib>Guan, Wei-jie</creatorcontrib><creatorcontrib>Jiang, Li-jie</creatorcontrib><creatorcontrib>Deng, Jie</creatorcontrib><creatorcontrib>Gao, Wen-xiang</creatorcontrib><creatorcontrib>Lee, Yew Mun</creatorcontrib><creatorcontrib>Xu, Zhao-feng</creatorcontrib><creatorcontrib>Luo, Xin</creatorcontrib><creatorcontrib>Liu, Chen</creatorcontrib><creatorcontrib>Shi, Jian-bo</creatorcontrib><creatorcontrib>Lai, Yinyan</creatorcontrib><title>Proteomics profiling of epithelium-derived exosomes from nasal polyps revealed signaling functions affecting cellular proliferation</title><title>Respiratory medicine</title><addtitle>Respir Med</addtitle><description>Nasal polyps are a significantly associated pathology of chronic rhinosinusitis (CRS) whose mechanisms of pathogenesis are not fully elucidated, especially the interaction of the polyp with its environment that allows its growth on the nasal epithelial lining. Exosomes are nanovesicles that serve important biological functions, including cell-to-cell signaling and communication.
Hence, we sought to explore the roles of the epithelial-derived exosomal proteome obtained from the human nasal epithelium in the modulation of CRS with nasal polyp (CRSwNP) pathogenesis.
We sampled exosomes from nasal lavage fluid and primary human nasal epithelial cells (hNECs) from healthy controls and patients with CRSwNP with and without coexisting asthma. The presence of exosomes was confirmed using a NanoSight assay, transmission electron microscopy and western blotting. The exosomal proteome was profiled with mass spectrometry. The Cell Counting Kit-8 was used to confirm the roles of exosomes in mediating cellular proliferation.
The hNEC-derived exosomes from diseased epithelium contained differentially expressed proteins that were mainly involved in epithelial remodeling via pathways such as p53. An in vitro study further demonstrated that epithelial-derived exosomes from patients with CRSwNP (with and without coexisting asthma) significantly reduced the rate of proliferation of control hNECs at an effective concentration of ≥10 μg/ml.
Exosomes secreted by hNECs from patients with CRSwNP, regardless of their coexistence with asthma, are laden with proteins that influence cell proliferation pathways, potentially leading to remodeling of the sinonasal mucosa.
Diseased (CRSwNP with and without coexisting asthma) human nasal epithelial cells (hNECs) were found to communicate with the environment via exosomes. Both healthy and exosomal protein signatures include immune response cytokines. Diseased epithelium-derived exosomes secreted increased cell cycle signatures involved in such as p53 pathway that might contribute to the inhibition of healthy epithelial proliferation, potentially as a mechanism of remodeling. [Display omitted]</description><subject>Antibiotics</subject><subject>Asthma</subject><subject>Cell proliferation</subject><subject>Chronic rhinosinusitis</subject><subject>Epithelial cells</subject><subject>Epithelium</subject><subject>Exosomes</subject><subject>Fluorides</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Medical diagnosis</subject><subject>Membranes</subject><subject>Mucosa</subject><subject>Nasal polyps</subject><subject>Nonsteroidal anti-inflammatory drugs</subject><subject>p53 Protein</subject><subject>Patients</subject><subject>Polyps</subject><subject>Proliferation</subject><subject>Proteins</subject><subject>Proteomes</subject><subject>Proteomics</subject><subject>Rhinitis</subject><subject>Rhinosinusitis</subject><subject>Scientific imaging</subject><subject>Signal transduction</subject><subject>Sinusitis</subject><subject>Transmission electron microscopy</subject><subject>Western blotting</subject><issn>0954-6111</issn><issn>1532-3064</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp9kU1v3CAQhlHVqNmm_QM9VEi99OItYMBY6qWK-iVFSg7tGWE8pKywccFeNef88UI2ufTQyzAannkH5kXoDSV7Sqj8cNinCcY9I6wWhOroM7SjomVNSyR_jnakF7yRlNJz9DLnAyGk55y8QOctI0LKju3Q_U2KK8TJ24yXFJ0Pfr7F0WFY_PoLgt-mZoTkjzBi-BNznCBjl-KEZ5NNwEsMd0vGCY5gQmGyv53Ng4bbZrv6OGdsnIOSlpqFELZgUh0VvINkKvEKnTkTMrx-PC_Qzy-ff1x-a66uv36__HTVWN7ztXFcyK7liijl-lbZgQ5W9bZG4KqnHbWDs6QHwg1I1XZ8EMCgld0oJBBoL9D7k26Z_nuDvOrJ5_okM0PcsmatED2XiqmCvvsHPcQtlZ89UIxwIURXKHaibIo5J3B6SX4y6U5ToqtF-qCrRbpapE8Wlaa3j9LbUO-eWp48KcDHEwBlF0cPSWfrYbYw-lT2qMfo_6f_F6tDpQs</recordid><startdate>202002</startdate><enddate>202002</enddate><creator>Zhou, Min</creator><creator>Tan, Kai Sen</creator><creator>Guan, Wei-jie</creator><creator>Jiang, Li-jie</creator><creator>Deng, Jie</creator><creator>Gao, Wen-xiang</creator><creator>Lee, Yew Mun</creator><creator>Xu, Zhao-feng</creator><creator>Luo, Xin</creator><creator>Liu, Chen</creator><creator>Shi, Jian-bo</creator><creator>Lai, Yinyan</creator><general>Elsevier Ltd</general><general>Elsevier Limited</general><scope>6I.</scope><scope>AAFTH</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>ASE</scope><scope>FPQ</scope><scope>H94</scope><scope>K6X</scope><scope>K9.</scope><scope>M7N</scope><scope>NAPCQ</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-4553-4383</orcidid><orcidid>https://orcid.org/0000-0003-2193-7612</orcidid></search><sort><creationdate>202002</creationdate><title>Proteomics profiling of epithelium-derived exosomes from nasal polyps revealed signaling functions affecting cellular proliferation</title><author>Zhou, Min ; Tan, Kai Sen ; Guan, Wei-jie ; Jiang, Li-jie ; Deng, Jie ; Gao, Wen-xiang ; Lee, Yew Mun ; Xu, Zhao-feng ; Luo, Xin ; Liu, Chen ; Shi, Jian-bo ; Lai, Yinyan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c494t-f4567348088f938cb1bc89c1bc8e489171cbfc09e04ae68374b5e2e367d56e0e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Antibiotics</topic><topic>Asthma</topic><topic>Cell proliferation</topic><topic>Chronic rhinosinusitis</topic><topic>Epithelial cells</topic><topic>Epithelium</topic><topic>Exosomes</topic><topic>Fluorides</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>Medical diagnosis</topic><topic>Membranes</topic><topic>Mucosa</topic><topic>Nasal polyps</topic><topic>Nonsteroidal anti-inflammatory drugs</topic><topic>p53 Protein</topic><topic>Patients</topic><topic>Polyps</topic><topic>Proliferation</topic><topic>Proteins</topic><topic>Proteomes</topic><topic>Proteomics</topic><topic>Rhinitis</topic><topic>Rhinosinusitis</topic><topic>Scientific imaging</topic><topic>Signal transduction</topic><topic>Sinusitis</topic><topic>Transmission electron microscopy</topic><topic>Western blotting</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhou, Min</creatorcontrib><creatorcontrib>Tan, Kai Sen</creatorcontrib><creatorcontrib>Guan, Wei-jie</creatorcontrib><creatorcontrib>Jiang, Li-jie</creatorcontrib><creatorcontrib>Deng, Jie</creatorcontrib><creatorcontrib>Gao, Wen-xiang</creatorcontrib><creatorcontrib>Lee, Yew Mun</creatorcontrib><creatorcontrib>Xu, Zhao-feng</creatorcontrib><creatorcontrib>Luo, Xin</creatorcontrib><creatorcontrib>Liu, Chen</creatorcontrib><creatorcontrib>Shi, Jian-bo</creatorcontrib><creatorcontrib>Lai, Yinyan</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>British Nursing Index</collection><collection>British Nursing Index (BNI) (1985 to Present)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>British Nursing Index</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Nursing & Allied Health Premium</collection><collection>MEDLINE - Academic</collection><jtitle>Respiratory medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhou, Min</au><au>Tan, Kai Sen</au><au>Guan, Wei-jie</au><au>Jiang, Li-jie</au><au>Deng, Jie</au><au>Gao, Wen-xiang</au><au>Lee, Yew Mun</au><au>Xu, Zhao-feng</au><au>Luo, Xin</au><au>Liu, Chen</au><au>Shi, Jian-bo</au><au>Lai, Yinyan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Proteomics profiling of epithelium-derived exosomes from nasal polyps revealed signaling functions affecting cellular proliferation</atitle><jtitle>Respiratory medicine</jtitle><addtitle>Respir Med</addtitle><date>2020-02</date><risdate>2020</risdate><volume>162</volume><spage>105871</spage><epage>105871</epage><pages>105871-105871</pages><artnum>105871</artnum><issn>0954-6111</issn><eissn>1532-3064</eissn><abstract>Nasal polyps are a significantly associated pathology of chronic rhinosinusitis (CRS) whose mechanisms of pathogenesis are not fully elucidated, especially the interaction of the polyp with its environment that allows its growth on the nasal epithelial lining. Exosomes are nanovesicles that serve important biological functions, including cell-to-cell signaling and communication.
Hence, we sought to explore the roles of the epithelial-derived exosomal proteome obtained from the human nasal epithelium in the modulation of CRS with nasal polyp (CRSwNP) pathogenesis.
We sampled exosomes from nasal lavage fluid and primary human nasal epithelial cells (hNECs) from healthy controls and patients with CRSwNP with and without coexisting asthma. The presence of exosomes was confirmed using a NanoSight assay, transmission electron microscopy and western blotting. The exosomal proteome was profiled with mass spectrometry. The Cell Counting Kit-8 was used to confirm the roles of exosomes in mediating cellular proliferation.
The hNEC-derived exosomes from diseased epithelium contained differentially expressed proteins that were mainly involved in epithelial remodeling via pathways such as p53. An in vitro study further demonstrated that epithelial-derived exosomes from patients with CRSwNP (with and without coexisting asthma) significantly reduced the rate of proliferation of control hNECs at an effective concentration of ≥10 μg/ml.
Exosomes secreted by hNECs from patients with CRSwNP, regardless of their coexistence with asthma, are laden with proteins that influence cell proliferation pathways, potentially leading to remodeling of the sinonasal mucosa.
Diseased (CRSwNP with and without coexisting asthma) human nasal epithelial cells (hNECs) were found to communicate with the environment via exosomes. Both healthy and exosomal protein signatures include immune response cytokines. Diseased epithelium-derived exosomes secreted increased cell cycle signatures involved in such as p53 pathway that might contribute to the inhibition of healthy epithelial proliferation, potentially as a mechanism of remodeling. [Display omitted]</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>32056672</pmid><doi>10.1016/j.rmed.2020.105871</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0003-4553-4383</orcidid><orcidid>https://orcid.org/0000-0003-2193-7612</orcidid><oa>free_for_read</oa></addata></record> |
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subjects | Antibiotics Asthma Cell proliferation Chronic rhinosinusitis Epithelial cells Epithelium Exosomes Fluorides Mass spectrometry Mass spectroscopy Medical diagnosis Membranes Mucosa Nasal polyps Nonsteroidal anti-inflammatory drugs p53 Protein Patients Polyps Proliferation Proteins Proteomes Proteomics Rhinitis Rhinosinusitis Scientific imaging Signal transduction Sinusitis Transmission electron microscopy Western blotting |
title | Proteomics profiling of epithelium-derived exosomes from nasal polyps revealed signaling functions affecting cellular proliferation |
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