Assessment of transmission risk in WSSV‐infected shrimp Litopenaeus vannamei upon cooking
White spot syndrome virus has been a threat to the global shrimp industry since it was discovered in Taiwan in 1992. Thus, shrimp‐producing countries have launched regulations to prevent import of WSSV‐infected commodity shrimp from endemic areas. Recently, cooked shrimp that is infected with WSSV t...
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Veröffentlicht in: | Journal of fish diseases 2020-04, Vol.43 (4), p.403-411 |
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creator | Aranguren Caro, Luis Fernando Mai, Hung N. Nunan, Linda Lin, Joshua Noble, Brenda Dhar, Arun K. |
description | White spot syndrome virus has been a threat to the global shrimp industry since it was discovered in Taiwan in 1992. Thus, shrimp‐producing countries have launched regulations to prevent import of WSSV‐infected commodity shrimp from endemic areas. Recently, cooked shrimp that is infected with WSSV tested positive by PCR. However, there is no study to determine the infectivity of WSSV in cooked shrimp that tested positive by PCR. In the present study, WSSV‐infected shrimp were cooked at boiling temperature for different times including 0, 1, 3, 5, 10 and 30 min. Upon exposure to boiling temperature, WSSV‐infected shrimp were fed to SPF shrimp (Litopenaeus vannamei). The result showed experimentally challenged shrimp from 0‐min treatment (positive control) indeed got infected with WSSV. However, experimentally challenged shrimp that were fed tissues boiled at 1, 3, 5, 10 and 30 min were not infected with WSSV. Mortality data showed that only the positive control (0‐min) treatment displayed high mortality, whereas no mortality was observed in any other treatment category. These findings suggest that cooking shrimp at boiling temperature for at least 1 min might prevent any potential spread of WSSV from endemic countries to other geographical areas where WSSV has not yet been reported. |
doi_str_mv | 10.1111/jfd.13128 |
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Thus, shrimp‐producing countries have launched regulations to prevent import of WSSV‐infected commodity shrimp from endemic areas. Recently, cooked shrimp that is infected with WSSV tested positive by PCR. However, there is no study to determine the infectivity of WSSV in cooked shrimp that tested positive by PCR. In the present study, WSSV‐infected shrimp were cooked at boiling temperature for different times including 0, 1, 3, 5, 10 and 30 min. Upon exposure to boiling temperature, WSSV‐infected shrimp were fed to SPF shrimp (Litopenaeus vannamei). The result showed experimentally challenged shrimp from 0‐min treatment (positive control) indeed got infected with WSSV. However, experimentally challenged shrimp that were fed tissues boiled at 1, 3, 5, 10 and 30 min were not infected with WSSV. Mortality data showed that only the positive control (0‐min) treatment displayed high mortality, whereas no mortality was observed in any other treatment category. These findings suggest that cooking shrimp at boiling temperature for at least 1 min might prevent any potential spread of WSSV from endemic countries to other geographical areas where WSSV has not yet been reported.</description><identifier>ISSN: 0140-7775</identifier><identifier>EISSN: 1365-2761</identifier><identifier>DOI: 10.1111/jfd.13128</identifier><identifier>PMID: 32048310</identifier><language>eng</language><publisher>England: Blackwell Publishing Ltd</publisher><subject>Boiling ; Cooking ; Crustaceans ; Decapoda ; DNA ; Health risks ; Industry ; Infectivity ; Litopenaeus vannamei ; Mortality ; nested PCR ; Nucleotide sequence ; PCR ; qPCR ; Shrimp fisheries ; Temperature ; Viruses ; White spot syndrome ; White spot syndrome virus ; white spot syndrome virus (WSSV)</subject><ispartof>Journal of fish diseases, 2020-04, Vol.43 (4), p.403-411</ispartof><rights>2020 John Wiley & Sons Ltd</rights><rights>2020 John Wiley & Sons Ltd.</rights><rights>Copyright © 2020 John Wiley & Sons Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3538-578dcc13a918fea8274e7b92aacb230818148995e61a5349d725bfaeb1336d883</citedby><cites>FETCH-LOGICAL-c3538-578dcc13a918fea8274e7b92aacb230818148995e61a5349d725bfaeb1336d883</cites><orcidid>0000-0002-4126-9431</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjfd.13128$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjfd.13128$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32048310$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Aranguren Caro, Luis Fernando</creatorcontrib><creatorcontrib>Mai, Hung N.</creatorcontrib><creatorcontrib>Nunan, Linda</creatorcontrib><creatorcontrib>Lin, Joshua</creatorcontrib><creatorcontrib>Noble, Brenda</creatorcontrib><creatorcontrib>Dhar, Arun K.</creatorcontrib><title>Assessment of transmission risk in WSSV‐infected shrimp Litopenaeus vannamei upon cooking</title><title>Journal of fish diseases</title><addtitle>J Fish Dis</addtitle><description>White spot syndrome virus has been a threat to the global shrimp industry since it was discovered in Taiwan in 1992. Thus, shrimp‐producing countries have launched regulations to prevent import of WSSV‐infected commodity shrimp from endemic areas. Recently, cooked shrimp that is infected with WSSV tested positive by PCR. However, there is no study to determine the infectivity of WSSV in cooked shrimp that tested positive by PCR. In the present study, WSSV‐infected shrimp were cooked at boiling temperature for different times including 0, 1, 3, 5, 10 and 30 min. Upon exposure to boiling temperature, WSSV‐infected shrimp were fed to SPF shrimp (Litopenaeus vannamei). The result showed experimentally challenged shrimp from 0‐min treatment (positive control) indeed got infected with WSSV. However, experimentally challenged shrimp that were fed tissues boiled at 1, 3, 5, 10 and 30 min were not infected with WSSV. Mortality data showed that only the positive control (0‐min) treatment displayed high mortality, whereas no mortality was observed in any other treatment category. These findings suggest that cooking shrimp at boiling temperature for at least 1 min might prevent any potential spread of WSSV from endemic countries to other geographical areas where WSSV has not yet been reported.</description><subject>Boiling</subject><subject>Cooking</subject><subject>Crustaceans</subject><subject>Decapoda</subject><subject>DNA</subject><subject>Health risks</subject><subject>Industry</subject><subject>Infectivity</subject><subject>Litopenaeus vannamei</subject><subject>Mortality</subject><subject>nested PCR</subject><subject>Nucleotide sequence</subject><subject>PCR</subject><subject>qPCR</subject><subject>Shrimp fisheries</subject><subject>Temperature</subject><subject>Viruses</subject><subject>White spot syndrome</subject><subject>White spot syndrome virus</subject><subject>white spot syndrome virus (WSSV)</subject><issn>0140-7775</issn><issn>1365-2761</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp1kMtO3TAQhq2qqJxSFn2BylI3ZRHw2HHsLBGFXnQkFvSyYBE5yaT4cGKHTAJixyP0GfskuBzKAqmzmc03v-b_GHsLYh_SHKy6dh8USPuCLUAVOpOmgJdsISAXmTFGb7PXRCshwGgoXrFtJUVuFYgFOz8kQqIew8Rjx6fRBeo9kY-Bj54uuQ_859nZjz93v33osJmw5XQx-n7gSz_FAYPDmfi1C8H16Pk8pMMmxksffr1hW51bE-4-7h32_eT429HnbHn66cvR4TJrlFY208a2TQPKlWA7dFaaHE1dSueaWiphwUJuy1JjAU6rvGyN1HXnsAalitZatcM-bHKHMV7NSFOVGjS4XruAcaZKKp2DljLXCX3_DF3FeQzpu0QlaQBCyETtbahmjEQjdtWQGrvxtgJR_TVeJePVg_HEvntMnOse2yfyn-IEHGyAG7_G2_8nVV9PPm4i7wEIkop0</recordid><startdate>202004</startdate><enddate>202004</enddate><creator>Aranguren Caro, Luis Fernando</creator><creator>Mai, Hung N.</creator><creator>Nunan, Linda</creator><creator>Lin, Joshua</creator><creator>Noble, Brenda</creator><creator>Dhar, Arun K.</creator><general>Blackwell Publishing Ltd</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TN</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H94</scope><scope>H95</scope><scope>H98</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-4126-9431</orcidid></search><sort><creationdate>202004</creationdate><title>Assessment of transmission risk in WSSV‐infected shrimp Litopenaeus vannamei upon cooking</title><author>Aranguren Caro, Luis Fernando ; Mai, Hung N. ; Nunan, Linda ; Lin, Joshua ; Noble, Brenda ; Dhar, Arun K.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3538-578dcc13a918fea8274e7b92aacb230818148995e61a5349d725bfaeb1336d883</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Boiling</topic><topic>Cooking</topic><topic>Crustaceans</topic><topic>Decapoda</topic><topic>DNA</topic><topic>Health risks</topic><topic>Industry</topic><topic>Infectivity</topic><topic>Litopenaeus vannamei</topic><topic>Mortality</topic><topic>nested PCR</topic><topic>Nucleotide sequence</topic><topic>PCR</topic><topic>qPCR</topic><topic>Shrimp fisheries</topic><topic>Temperature</topic><topic>Viruses</topic><topic>White spot syndrome</topic><topic>White spot syndrome virus</topic><topic>white spot syndrome virus (WSSV)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Aranguren Caro, Luis Fernando</creatorcontrib><creatorcontrib>Mai, Hung N.</creatorcontrib><creatorcontrib>Nunan, Linda</creatorcontrib><creatorcontrib>Lin, Joshua</creatorcontrib><creatorcontrib>Noble, Brenda</creatorcontrib><creatorcontrib>Dhar, Arun K.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Oceanic Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of fish diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Aranguren Caro, Luis Fernando</au><au>Mai, Hung N.</au><au>Nunan, Linda</au><au>Lin, Joshua</au><au>Noble, Brenda</au><au>Dhar, Arun K.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Assessment of transmission risk in WSSV‐infected shrimp Litopenaeus vannamei upon cooking</atitle><jtitle>Journal of fish diseases</jtitle><addtitle>J Fish Dis</addtitle><date>2020-04</date><risdate>2020</risdate><volume>43</volume><issue>4</issue><spage>403</spage><epage>411</epage><pages>403-411</pages><issn>0140-7775</issn><eissn>1365-2761</eissn><abstract>White spot syndrome virus has been a threat to the global shrimp industry since it was discovered in Taiwan in 1992. Thus, shrimp‐producing countries have launched regulations to prevent import of WSSV‐infected commodity shrimp from endemic areas. Recently, cooked shrimp that is infected with WSSV tested positive by PCR. However, there is no study to determine the infectivity of WSSV in cooked shrimp that tested positive by PCR. In the present study, WSSV‐infected shrimp were cooked at boiling temperature for different times including 0, 1, 3, 5, 10 and 30 min. Upon exposure to boiling temperature, WSSV‐infected shrimp were fed to SPF shrimp (Litopenaeus vannamei). The result showed experimentally challenged shrimp from 0‐min treatment (positive control) indeed got infected with WSSV. However, experimentally challenged shrimp that were fed tissues boiled at 1, 3, 5, 10 and 30 min were not infected with WSSV. Mortality data showed that only the positive control (0‐min) treatment displayed high mortality, whereas no mortality was observed in any other treatment category. 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subjects | Boiling Cooking Crustaceans Decapoda DNA Health risks Industry Infectivity Litopenaeus vannamei Mortality nested PCR Nucleotide sequence PCR qPCR Shrimp fisheries Temperature Viruses White spot syndrome White spot syndrome virus white spot syndrome virus (WSSV) |
title | Assessment of transmission risk in WSSV‐infected shrimp Litopenaeus vannamei upon cooking |
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