Cloning and expression of the Bacillus amyloliquefaciens transglutaminase gene in E. coli using a bicistronic vector construction

[Display omitted] •Cloning and expression of recombinant B. amyloliquefaciens TGase in E. coli.•Protein was obtained in its soluble and active form.•A bicistronic plasmid was constructed to avoid host cells toxicity.•TGase gene was fused to the inhibitory S. caniferus prodomain.•We cloned the 3C pro...

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Veröffentlicht in:	Enzyme and microbial technology 2020-03, Vol.134, p.109468-109468, Article 109468
Hauptverfasser:	Duarte, Lovaine Silva, Barsé, Laísa Quadros, Dalberto, Pedro Ferrari, da Silva, William Tadeu Santos, Rodrigues, Rafael Costa, Machado, Pablo, Basso, Luiz Augusto, Bizarro, Cristiano Valim, Ayub, Marco Antônio Záchia
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacillus amyloliquefaciens Food enzymes Microbial transglutaminase Protein cross-linking Transglutaminase
Online-Zugang:	Volltext
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