Optimizing Nervous System-Specific Gene Targeting with Cre Driver Lines: Prevalence of Germline Recombination and Influencing Factors

The Cre-loxP system is invaluable for spatial and temporal control of gene knockout, knockin, and reporter expression in the mouse nervous system. However, we report varying probabilities of unexpected germline recombination in distinct Cre driver lines designed for nervous system-specific recombina...

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Veröffentlicht in:	Neuron (Cambridge, Mass.) Mass.), 2020-04, Vol.106 (1), p.37-65.e5
Hauptverfasser:	Luo, Lin, Ambrozkiewicz, Mateusz C., Benseler, Fritz, Chen, Cui, Dumontier, Emilie, Falkner, Susanne, Furlanis, Elisabetta, Gomez, Andrea M., Hoshina, Naosuke, Huang, Wei-Hsiang, Hutchison, Mary Anne, Itoh-Maruoka, Yu, Lavery, Laura A., Li, Wei, Maruo, Tomohiko, Motohashi, Junko, Pai, Emily Ling-Lin, Pelkey, Kenneth A., Pereira, Ariane, Philips, Thomas, Sinclair, Jennifer L., Stogsdill, Jeff A., Traunmüller, Lisa, Wang, Jiexin, Wortel, Joke, You, Wenjia, Abumaria, Nashat, Beier, Kevin T., Brose, Nils, Burgess, Harold A., Cepko, Constance L., Cloutier, Jean-François, Eroglu, Cagla, Goebbels, Sandra, Kaeser, Pascal S., Kay, Jeremy N., Lu, Wei, Luo, Liqun, Mandai, Kenji, McBain, Chris J., Nave, Klaus-Armin, Prado, Marco A.M., Prado, Vania F., Rothstein, Jeffrey, Rubenstein, John L.R., Saher, Gesine, Sakimura, Kenji, Sanes, Joshua R., Scheiffele, Peter, Takai, Yoshimi, Umemori, Hisashi, Verhage, Matthijs, Yuzaki, Michisuke, Zoghbi, Huda Yahya, Kawabe, Hiroshi, Craig, Ann Marie
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals conditional gene targeting conditional knockin conditional knockout conditional reporter Cre-lox Female Gene expression Gene targeting Gene Targeting - methods Genes, Reporter Genetic engineering Germ Cells germline recombination Integrases - genetics Male Mice Mice, Transgenic molecular genetics mosaic recombination Mosaicism Nervous system Neurons - metabolism Oocytes Oocytes - metabolism parental sex bias Recombinase Recombination Recombination, Genetic - genetics Rodents site-specific recombinase Spermatozoa - metabolism Transcription Transgenic animals
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