Miniature and fully portable gradient capillary liquid chromatograph
A robust, portable and miniature battery powered gradient capillary liquid chromatograph (total weight ∼2.7 kg, without battery ∼2.0 kg), with integrated microfluidic injection, column heating and high sensitivity low-UV absorbance detection is presented. The portable capillary chromatograph, was ap...
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| Veröffentlicht in: | Analytica chimica acta 2020-03, Vol.1101, p.199-210 |
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| container_title | Analytica chimica acta |
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| creator | Lam, Shing Chung Coates, Lewellwyn Joseph Hemida, Mohamed Gupta, Vipul Haddad, Paul R. Macka, Mirek Paull, Brett |
| description | A robust, portable and miniature battery powered gradient capillary liquid chromatograph (total weight ∼2.7 kg, without battery ∼2.0 kg), with integrated microfluidic injection, column heating and high sensitivity low-UV absorbance detection is presented. The portable capillary chromatograph, was applied with a packed reversed-phase capillary column (100 mm × 300 μm I.D., 5 μm ODS), housed within an integrated capillary column heater controlled by a proportional-integral-derivative (PID) chip module. The system delivered retention time and peak area relative standard deviation in isocratic mode of |
| doi_str_mv | 10.1016/j.aca.2019.12.014 |
| format | Article |
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[Display omitted]
•A hand-portable (2.7 kg) and battery-operated liquid chromatograph is presented.•Highly repeatable gradient capillary chromatography with UV absorbance detection.•Developed UV-LED based z-cell provides linearity up to 1.6 AU with low stray light of ∼0.2%.•Demonstrated coupled with miniature single quadrupole mass spectrometer.</description><identifier>ISSN: 0003-2670</identifier><identifier>EISSN: 1873-4324</identifier><identifier>DOI: 10.1016/j.aca.2019.12.014</identifier><identifier>PMID: 32029112</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Capillary liquid chromatography ; Column heater ; Miniaturisation ; Portable ; Portable mass spectrometer ; Ultraviolet light-emitting diode-based detector</subject><ispartof>Analytica chimica acta, 2020-03, Vol.1101, p.199-210</ispartof><rights>2019 Elsevier B.V.</rights><rights>Copyright © 2019 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c419t-9067ec94ccb434bca9992318edc66af346d82f13f8cf191d9c730409ac8a23203</citedby><cites>FETCH-LOGICAL-c419t-9067ec94ccb434bca9992318edc66af346d82f13f8cf191d9c730409ac8a23203</cites><orcidid>0000-0001-9579-7363 ; 0000-0001-5247-8397 ; 0000-0001-6373-6582 ; 0000-0002-6792-2574</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.aca.2019.12.014$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3541,27915,27916,45986</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32029112$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lam, Shing Chung</creatorcontrib><creatorcontrib>Coates, Lewellwyn Joseph</creatorcontrib><creatorcontrib>Hemida, Mohamed</creatorcontrib><creatorcontrib>Gupta, Vipul</creatorcontrib><creatorcontrib>Haddad, Paul R.</creatorcontrib><creatorcontrib>Macka, Mirek</creatorcontrib><creatorcontrib>Paull, Brett</creatorcontrib><title>Miniature and fully portable gradient capillary liquid chromatograph</title><title>Analytica chimica acta</title><addtitle>Anal Chim Acta</addtitle><description>A robust, portable and miniature battery powered gradient capillary liquid chromatograph (total weight ∼2.7 kg, without battery ∼2.0 kg), with integrated microfluidic injection, column heating and high sensitivity low-UV absorbance detection is presented. The portable capillary chromatograph, was applied with a packed reversed-phase capillary column (100 mm × 300 μm I.D., 5 μm ODS), housed within an integrated capillary column heater controlled by a proportional-integral-derivative (PID) chip module. The system delivered retention time and peak area relative standard deviation in isocratic mode of <0.7% (n = 10) and <3.3% (n = 10), respectively, and <0.1% (n = 10) and <2.3% (n = 10) respectively, for gradient elution mode. Detection was based upon a 255 nm light-emitting diode (LED) using one of two commercial capillary flow-cell options, namely a high sensitivity 12 nL Agilent capillary z-cell (HSDC) and a 45 nL Thermo Fisher Scientific UZ-View™ flow cell (UZFC). The HSDC, housed within a 3D printed detector arrangement, gave an effective pathlength of 1.01 mm (84% of nominal pathlength) and stray light of only 0.2%. Limits of detection for four test small molecule pharmaceuticals ranged from 65 to 101 μg L−1 based upon a 316 nL injection volume, with separation efficiencies of between 18,000 and 29,700 N m−1, with sub-4 min run times. The portable capillary LC system was successfully coupled to a small footprint portable mass spectrometer (Microsaic 4500 MiD) to demonstrate compatibility and ‘point-of-need’ miniaturised LC-MS capability.
[Display omitted]
•A hand-portable (2.7 kg) and battery-operated liquid chromatograph is presented.•Highly repeatable gradient capillary chromatography with UV absorbance detection.•Developed UV-LED based z-cell provides linearity up to 1.6 AU with low stray light of ∼0.2%.•Demonstrated coupled with miniature single quadrupole mass spectrometer.</description><subject>Capillary liquid chromatography</subject><subject>Column heater</subject><subject>Miniaturisation</subject><subject>Portable</subject><subject>Portable mass spectrometer</subject><subject>Ultraviolet light-emitting diode-based detector</subject><issn>0003-2670</issn><issn>1873-4324</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp9kE1PwzAMhiMEYmPwA7igHrm0xEloG3FC41Ma4gLnKHVSlqldu6RF2r8n0wZHfLEsPX5lP4RcAs2AQn6zyjTqjFGQGbCMgjgiUygLngrOxDGZUkp5yvKCTshZCKs4MqDilEw4o0wCsCl5eHNrp4fR20SvTVKPTbNN-s4Pumps8uW1cXY9JKh71zTab5PGbUZnElz6rtVDF4l-eU5Oat0Ee3HoM_L59Pgxf0kX78-v8_tFigLkkEqaFxalQKwEFxVqKSXjUFqDea5rLnJTshp4XWINEozEglNBpcZSs3gyn5HrfW7vu81ow6BaF9DGw9a2G4Ni_JblPFYeUdij6LsQvK1V710bH1BA1U6eWqkoT-3kKWAqyos7V4f4sWqt-dv4tRWBuz1g45PfznoVMOpBa5y3OCjTuX_ifwDxOH8D</recordid><startdate>20200308</startdate><enddate>20200308</enddate><creator>Lam, Shing Chung</creator><creator>Coates, Lewellwyn Joseph</creator><creator>Hemida, Mohamed</creator><creator>Gupta, Vipul</creator><creator>Haddad, Paul R.</creator><creator>Macka, Mirek</creator><creator>Paull, Brett</creator><general>Elsevier B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-9579-7363</orcidid><orcidid>https://orcid.org/0000-0001-5247-8397</orcidid><orcidid>https://orcid.org/0000-0001-6373-6582</orcidid><orcidid>https://orcid.org/0000-0002-6792-2574</orcidid></search><sort><creationdate>20200308</creationdate><title>Miniature and fully portable gradient capillary liquid chromatograph</title><author>Lam, Shing Chung ; Coates, Lewellwyn Joseph ; Hemida, Mohamed ; Gupta, Vipul ; Haddad, Paul R. ; Macka, Mirek ; Paull, Brett</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c419t-9067ec94ccb434bca9992318edc66af346d82f13f8cf191d9c730409ac8a23203</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Capillary liquid chromatography</topic><topic>Column heater</topic><topic>Miniaturisation</topic><topic>Portable</topic><topic>Portable mass spectrometer</topic><topic>Ultraviolet light-emitting diode-based detector</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lam, Shing Chung</creatorcontrib><creatorcontrib>Coates, Lewellwyn Joseph</creatorcontrib><creatorcontrib>Hemida, Mohamed</creatorcontrib><creatorcontrib>Gupta, Vipul</creatorcontrib><creatorcontrib>Haddad, Paul R.</creatorcontrib><creatorcontrib>Macka, Mirek</creatorcontrib><creatorcontrib>Paull, Brett</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Analytica chimica acta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lam, Shing Chung</au><au>Coates, Lewellwyn Joseph</au><au>Hemida, Mohamed</au><au>Gupta, Vipul</au><au>Haddad, Paul R.</au><au>Macka, Mirek</au><au>Paull, Brett</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Miniature and fully portable gradient capillary liquid chromatograph</atitle><jtitle>Analytica chimica acta</jtitle><addtitle>Anal Chim Acta</addtitle><date>2020-03-08</date><risdate>2020</risdate><volume>1101</volume><spage>199</spage><epage>210</epage><pages>199-210</pages><issn>0003-2670</issn><eissn>1873-4324</eissn><abstract>A robust, portable and miniature battery powered gradient capillary liquid chromatograph (total weight ∼2.7 kg, without battery ∼2.0 kg), with integrated microfluidic injection, column heating and high sensitivity low-UV absorbance detection is presented. The portable capillary chromatograph, was applied with a packed reversed-phase capillary column (100 mm × 300 μm I.D., 5 μm ODS), housed within an integrated capillary column heater controlled by a proportional-integral-derivative (PID) chip module. The system delivered retention time and peak area relative standard deviation in isocratic mode of <0.7% (n = 10) and <3.3% (n = 10), respectively, and <0.1% (n = 10) and <2.3% (n = 10) respectively, for gradient elution mode. Detection was based upon a 255 nm light-emitting diode (LED) using one of two commercial capillary flow-cell options, namely a high sensitivity 12 nL Agilent capillary z-cell (HSDC) and a 45 nL Thermo Fisher Scientific UZ-View™ flow cell (UZFC). The HSDC, housed within a 3D printed detector arrangement, gave an effective pathlength of 1.01 mm (84% of nominal pathlength) and stray light of only 0.2%. Limits of detection for four test small molecule pharmaceuticals ranged from 65 to 101 μg L−1 based upon a 316 nL injection volume, with separation efficiencies of between 18,000 and 29,700 N m−1, with sub-4 min run times. The portable capillary LC system was successfully coupled to a small footprint portable mass spectrometer (Microsaic 4500 MiD) to demonstrate compatibility and ‘point-of-need’ miniaturised LC-MS capability.
[Display omitted]
•A hand-portable (2.7 kg) and battery-operated liquid chromatograph is presented.•Highly repeatable gradient capillary chromatography with UV absorbance detection.•Developed UV-LED based z-cell provides linearity up to 1.6 AU with low stray light of ∼0.2%.•Demonstrated coupled with miniature single quadrupole mass spectrometer.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>32029112</pmid><doi>10.1016/j.aca.2019.12.014</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0001-9579-7363</orcidid><orcidid>https://orcid.org/0000-0001-5247-8397</orcidid><orcidid>https://orcid.org/0000-0001-6373-6582</orcidid><orcidid>https://orcid.org/0000-0002-6792-2574</orcidid></addata></record> |
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| subjects | Capillary liquid chromatography Column heater Miniaturisation Portable Portable mass spectrometer Ultraviolet light-emitting diode-based detector |
| title | Miniature and fully portable gradient capillary liquid chromatograph |
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