Differences in DNA methylation of insulin-like growth factor 2 and cadherin 13 in patients with preeclampsia
•Early-onset PE showed altered DNA methylation in placenta and umbilical cord.•There were no differences between late-onset PE and controls.•IGF-2 and cadherin 13 showed the most differential methylated CpGs.•This may even affect health and disease risks of newborns later in life. In a previous mass...
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| Veröffentlicht in: | Pregnancy hypertension 2020-01, Vol.19, p.150-158 |
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| creator | van den Berg, C.B. Herzog, E.M. Duvekot, J.J. van der Spek, P.J. Steegers, E.A.P. Stoop, M.P. Willemsen, S.P. Steegers-Theunissen, R.P.M. |
| description | •Early-onset PE showed altered DNA methylation in placenta and umbilical cord.•There were no differences between late-onset PE and controls.•IGF-2 and cadherin 13 showed the most differential methylated CpGs.•This may even affect health and disease risks of newborns later in life.
In a previous mass spectrometry study of our research group, 25 proteins were found to be differentially expressed in cerebrospinal fluid of patients with preeclampsia compared to controls. The objective of the current study was to investigate DNA methylation of the genes encoding for the former mentioned proteins in an independent dataset.
In a nested case-control study of the Rotterdam Periconceptional Cohort, placental tissue, umbilical cord white blood cells and human umbilical vein endothelial cells (HUVEC) were obtained of 13 patients with early-onset preeclampsia, 16 patients with late-onset preeclampsia and 83 normotensive controls (27 patients with fetal growth restriction, 20 patients with spontaneous preterm birth and 36 uncomplicated pregnancies). DNA methylation of 783 CpGs in regions of 25 genes was measured.
DNA methylation of selected candidate genes in early- and late-onset preeclampsia compared to fetal growth restriction, spontaneous preterm birth and uncomplicated controls.
From the 783 CpGs of the 25 selected genes, 15 CpGs were differentially methylated between early-onset preeclampsia and spontaneous preterm birth (3.80 E-5 ≤ p ≤ 0.036). Four CpGs were differentially methylated between early-onset preeclampsia and fetal growth restriction (0.0002 ≤ p ≤ 0.037) and 13 CpGs were differentially methylated between early onset preeclampsia and uncomplicated controls (0.0001 ≤ p ≤ 0.04).
Differences in DNA methylation were found in placental tissue, umbilical cord white blood cells and HUVEC of patients with early onset preeclampsia compared to (un)complicated controls, but not in patients with late-onset preeclampsia. The genes showing the largest differential methylation encode insulin-like growth factor 2 binding protein and receptor and cadherin 13. |
| doi_str_mv | 10.1016/j.preghy.2020.01.010 |
| format | Article |
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In a previous mass spectrometry study of our research group, 25 proteins were found to be differentially expressed in cerebrospinal fluid of patients with preeclampsia compared to controls. The objective of the current study was to investigate DNA methylation of the genes encoding for the former mentioned proteins in an independent dataset.
In a nested case-control study of the Rotterdam Periconceptional Cohort, placental tissue, umbilical cord white blood cells and human umbilical vein endothelial cells (HUVEC) were obtained of 13 patients with early-onset preeclampsia, 16 patients with late-onset preeclampsia and 83 normotensive controls (27 patients with fetal growth restriction, 20 patients with spontaneous preterm birth and 36 uncomplicated pregnancies). DNA methylation of 783 CpGs in regions of 25 genes was measured.
DNA methylation of selected candidate genes in early- and late-onset preeclampsia compared to fetal growth restriction, spontaneous preterm birth and uncomplicated controls.
From the 783 CpGs of the 25 selected genes, 15 CpGs were differentially methylated between early-onset preeclampsia and spontaneous preterm birth (3.80 E-5 ≤ p ≤ 0.036). Four CpGs were differentially methylated between early-onset preeclampsia and fetal growth restriction (0.0002 ≤ p ≤ 0.037) and 13 CpGs were differentially methylated between early onset preeclampsia and uncomplicated controls (0.0001 ≤ p ≤ 0.04).
Differences in DNA methylation were found in placental tissue, umbilical cord white blood cells and HUVEC of patients with early onset preeclampsia compared to (un)complicated controls, but not in patients with late-onset preeclampsia. The genes showing the largest differential methylation encode insulin-like growth factor 2 binding protein and receptor and cadherin 13.</description><identifier>ISSN: 2210-7789</identifier><identifier>EISSN: 2210-7797</identifier><identifier>DOI: 10.1016/j.preghy.2020.01.010</identifier><identifier>PMID: 32007784</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Adult ; Cadherins - genetics ; Case-Control Studies ; CpG Islands ; DNA Methylation ; Endothelial Cells - metabolism ; Female ; Fetal Blood - cytology ; Fetal Growth Retardation - genetics ; Humans ; HUVEC ; Insulin-Like Growth Factor II - genetics ; Leukocytes - metabolism ; Placenta ; Placenta - metabolism ; Pre-Eclampsia - genetics ; Preeclampsia ; Pregnancy ; Premature Birth - genetics ; Umbilical cord white blood cells ; Umbilical Veins - cytology</subject><ispartof>Pregnancy hypertension, 2020-01, Vol.19, p.150-158</ispartof><rights>2020 International Society for the Study of Hypertension in Pregnancy</rights><rights>Copyright © 2020 International Society for the Study of Hypertension in Pregnancy. Published by Elsevier B.V. All rights reserved.</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c362t-57366d76fd3bdc09de4407440cfe906fa9e16bf2a4aca5bd019e64c76a773cc83</citedby><cites>FETCH-LOGICAL-c362t-57366d76fd3bdc09de4407440cfe906fa9e16bf2a4aca5bd019e64c76a773cc83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.preghy.2020.01.010$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3549,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32007784$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>van den Berg, C.B.</creatorcontrib><creatorcontrib>Herzog, E.M.</creatorcontrib><creatorcontrib>Duvekot, J.J.</creatorcontrib><creatorcontrib>van der Spek, P.J.</creatorcontrib><creatorcontrib>Steegers, E.A.P.</creatorcontrib><creatorcontrib>Stoop, M.P.</creatorcontrib><creatorcontrib>Willemsen, S.P.</creatorcontrib><creatorcontrib>Steegers-Theunissen, R.P.M.</creatorcontrib><title>Differences in DNA methylation of insulin-like growth factor 2 and cadherin 13 in patients with preeclampsia</title><title>Pregnancy hypertension</title><addtitle>Pregnancy Hypertens</addtitle><description>•Early-onset PE showed altered DNA methylation in placenta and umbilical cord.•There were no differences between late-onset PE and controls.•IGF-2 and cadherin 13 showed the most differential methylated CpGs.•This may even affect health and disease risks of newborns later in life.
In a previous mass spectrometry study of our research group, 25 proteins were found to be differentially expressed in cerebrospinal fluid of patients with preeclampsia compared to controls. The objective of the current study was to investigate DNA methylation of the genes encoding for the former mentioned proteins in an independent dataset.
In a nested case-control study of the Rotterdam Periconceptional Cohort, placental tissue, umbilical cord white blood cells and human umbilical vein endothelial cells (HUVEC) were obtained of 13 patients with early-onset preeclampsia, 16 patients with late-onset preeclampsia and 83 normotensive controls (27 patients with fetal growth restriction, 20 patients with spontaneous preterm birth and 36 uncomplicated pregnancies). DNA methylation of 783 CpGs in regions of 25 genes was measured.
DNA methylation of selected candidate genes in early- and late-onset preeclampsia compared to fetal growth restriction, spontaneous preterm birth and uncomplicated controls.
From the 783 CpGs of the 25 selected genes, 15 CpGs were differentially methylated between early-onset preeclampsia and spontaneous preterm birth (3.80 E-5 ≤ p ≤ 0.036). Four CpGs were differentially methylated between early-onset preeclampsia and fetal growth restriction (0.0002 ≤ p ≤ 0.037) and 13 CpGs were differentially methylated between early onset preeclampsia and uncomplicated controls (0.0001 ≤ p ≤ 0.04).
Differences in DNA methylation were found in placental tissue, umbilical cord white blood cells and HUVEC of patients with early onset preeclampsia compared to (un)complicated controls, but not in patients with late-onset preeclampsia. The genes showing the largest differential methylation encode insulin-like growth factor 2 binding protein and receptor and cadherin 13.</description><subject>Adult</subject><subject>Cadherins - genetics</subject><subject>Case-Control Studies</subject><subject>CpG Islands</subject><subject>DNA Methylation</subject><subject>Endothelial Cells - metabolism</subject><subject>Female</subject><subject>Fetal Blood - cytology</subject><subject>Fetal Growth Retardation - genetics</subject><subject>Humans</subject><subject>HUVEC</subject><subject>Insulin-Like Growth Factor II - genetics</subject><subject>Leukocytes - metabolism</subject><subject>Placenta</subject><subject>Placenta - metabolism</subject><subject>Pre-Eclampsia - genetics</subject><subject>Preeclampsia</subject><subject>Pregnancy</subject><subject>Premature Birth - genetics</subject><subject>Umbilical cord white blood cells</subject><subject>Umbilical Veins - cytology</subject><issn>2210-7789</issn><issn>2210-7797</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtOAyEUhonRqNG-gTEs3Uw9wBQ6GxPjPTG60TWhcLDUuQlTm769NK0uJYdA4PvP5SfkjMGYAZOXi3Ef8WO-HnPgMAaWA_bIMecMCqUqtf93n1ZHZJTSAvIqJzBV8pAcCQ6Qv8pjUt8G7zFiazHR0NLbl2va4DBf12YIXUs7n1_Tsg5tUYdPpB-xWw1z6o0dukg5Na2j1rg5xixmYpOiz0psh0RXIZO5T7S1afoUzCk58KZOONqdJ-T9_u7t5rF4fn14url-LqyQfCgmSkjplPROzJyFymFZgsrbeqxAelMhkzPPTWmsmcwcsAplaZU0Sglrp-KEXGzz9rH7WmIadBOSxbo2LXbLpLmYgBAVYzKj5Ra1sUspotd9DI2Ja81Ab6zWC721Wm-s1sByQJad7yosZw26P9GvsRm42gKY5_wOGHWyYeOyCxHtoF0X_q_wA1mRkdo</recordid><startdate>202001</startdate><enddate>202001</enddate><creator>van den Berg, C.B.</creator><creator>Herzog, E.M.</creator><creator>Duvekot, J.J.</creator><creator>van der Spek, P.J.</creator><creator>Steegers, E.A.P.</creator><creator>Stoop, M.P.</creator><creator>Willemsen, S.P.</creator><creator>Steegers-Theunissen, R.P.M.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>202001</creationdate><title>Differences in DNA methylation of insulin-like growth factor 2 and cadherin 13 in patients with preeclampsia</title><author>van den Berg, C.B. ; Herzog, E.M. ; Duvekot, J.J. ; van der Spek, P.J. ; Steegers, E.A.P. ; Stoop, M.P. ; Willemsen, S.P. ; Steegers-Theunissen, R.P.M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c362t-57366d76fd3bdc09de4407440cfe906fa9e16bf2a4aca5bd019e64c76a773cc83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Adult</topic><topic>Cadherins - genetics</topic><topic>Case-Control Studies</topic><topic>CpG Islands</topic><topic>DNA Methylation</topic><topic>Endothelial Cells - metabolism</topic><topic>Female</topic><topic>Fetal Blood - cytology</topic><topic>Fetal Growth Retardation - genetics</topic><topic>Humans</topic><topic>HUVEC</topic><topic>Insulin-Like Growth Factor II - genetics</topic><topic>Leukocytes - metabolism</topic><topic>Placenta</topic><topic>Placenta - metabolism</topic><topic>Pre-Eclampsia - genetics</topic><topic>Preeclampsia</topic><topic>Pregnancy</topic><topic>Premature Birth - genetics</topic><topic>Umbilical cord white blood cells</topic><topic>Umbilical Veins - cytology</topic><toplevel>online_resources</toplevel><creatorcontrib>van den Berg, C.B.</creatorcontrib><creatorcontrib>Herzog, E.M.</creatorcontrib><creatorcontrib>Duvekot, J.J.</creatorcontrib><creatorcontrib>van der Spek, P.J.</creatorcontrib><creatorcontrib>Steegers, E.A.P.</creatorcontrib><creatorcontrib>Stoop, M.P.</creatorcontrib><creatorcontrib>Willemsen, S.P.</creatorcontrib><creatorcontrib>Steegers-Theunissen, R.P.M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Pregnancy hypertension</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>van den Berg, C.B.</au><au>Herzog, E.M.</au><au>Duvekot, J.J.</au><au>van der Spek, P.J.</au><au>Steegers, E.A.P.</au><au>Stoop, M.P.</au><au>Willemsen, S.P.</au><au>Steegers-Theunissen, R.P.M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differences in DNA methylation of insulin-like growth factor 2 and cadherin 13 in patients with preeclampsia</atitle><jtitle>Pregnancy hypertension</jtitle><addtitle>Pregnancy Hypertens</addtitle><date>2020-01</date><risdate>2020</risdate><volume>19</volume><spage>150</spage><epage>158</epage><pages>150-158</pages><issn>2210-7789</issn><eissn>2210-7797</eissn><abstract>•Early-onset PE showed altered DNA methylation in placenta and umbilical cord.•There were no differences between late-onset PE and controls.•IGF-2 and cadherin 13 showed the most differential methylated CpGs.•This may even affect health and disease risks of newborns later in life.
In a previous mass spectrometry study of our research group, 25 proteins were found to be differentially expressed in cerebrospinal fluid of patients with preeclampsia compared to controls. The objective of the current study was to investigate DNA methylation of the genes encoding for the former mentioned proteins in an independent dataset.
In a nested case-control study of the Rotterdam Periconceptional Cohort, placental tissue, umbilical cord white blood cells and human umbilical vein endothelial cells (HUVEC) were obtained of 13 patients with early-onset preeclampsia, 16 patients with late-onset preeclampsia and 83 normotensive controls (27 patients with fetal growth restriction, 20 patients with spontaneous preterm birth and 36 uncomplicated pregnancies). DNA methylation of 783 CpGs in regions of 25 genes was measured.
DNA methylation of selected candidate genes in early- and late-onset preeclampsia compared to fetal growth restriction, spontaneous preterm birth and uncomplicated controls.
From the 783 CpGs of the 25 selected genes, 15 CpGs were differentially methylated between early-onset preeclampsia and spontaneous preterm birth (3.80 E-5 ≤ p ≤ 0.036). Four CpGs were differentially methylated between early-onset preeclampsia and fetal growth restriction (0.0002 ≤ p ≤ 0.037) and 13 CpGs were differentially methylated between early onset preeclampsia and uncomplicated controls (0.0001 ≤ p ≤ 0.04).
Differences in DNA methylation were found in placental tissue, umbilical cord white blood cells and HUVEC of patients with early onset preeclampsia compared to (un)complicated controls, but not in patients with late-onset preeclampsia. The genes showing the largest differential methylation encode insulin-like growth factor 2 binding protein and receptor and cadherin 13.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>32007784</pmid><doi>10.1016/j.preghy.2020.01.010</doi><tpages>9</tpages></addata></record> |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Adult Cadherins - genetics Case-Control Studies CpG Islands DNA Methylation Endothelial Cells - metabolism Female Fetal Blood - cytology Fetal Growth Retardation - genetics Humans HUVEC Insulin-Like Growth Factor II - genetics Leukocytes - metabolism Placenta Placenta - metabolism Pre-Eclampsia - genetics Preeclampsia Pregnancy Premature Birth - genetics Umbilical cord white blood cells Umbilical Veins - cytology |
| title | Differences in DNA methylation of insulin-like growth factor 2 and cadherin 13 in patients with preeclampsia |
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