Staphylococcus aureus carrying lukS/F Panton-Valentine Leukocidin (PVL) toxin genes in hospitals of Lahore city
Panton Valentine-Leukocidin (PVL) toxin is secreted by Staphylococcus aureus and is mostly associated with skin and soft tissue infections (SSTI). This study aims to find out the prevalence of lukS/F-PV gene, which encode PVL toxin from strains of SSTI, burn wounds and nasal colonizers of out-patien...
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| Veröffentlicht in: | Journal of infection in developing countries 2018-09, Vol.12 (9), p.720-725 |
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| creator | Iqbal, Muhammad Shaheen Saleem, Yasar Ansari, Farheen Qamar, Muhammad Usman Mazhar, Sania Hassan, Abida Nawaz, Shaista Saeed, Salman Syed, Quratulain |
| description | Panton Valentine-Leukocidin (PVL) toxin is secreted by Staphylococcus aureus and is mostly associated with skin and soft tissue infections (SSTI). This study aims to find out the prevalence of lukS/F-PV gene, which encode PVL toxin from strains of SSTI, burn wounds and nasal colonizers of out-patients and to measure the antimicrobial susceptibility of S. aureus isolates.
This is an analytical observational cross-section study and was conducted from July 2014 to June 2015 at four tertiary care hospitals and PCSIR Laboratories Complex, Lahore, Pakistan. A total of 376 random clinical swabs were collected from SSTI (n = 179), nasal nares (n = 134) and burn wounds (n = 63) from out-patients' departments (OPD). The specimens were cultured on nutrient and mannitol salt agar (MSA) and the organism was identified by catalase, coagulase, and DNase tests. Antimicrobial susceptibility, methicillin, inducible clindamycin, and high-level mupirocin (HLMR) resistance were determined as per CLSI guidelines. Molecular identification of mecA and lukS/F-PV genes was performed by PCR.
We isolated 127 S. aureus, where 41 (32.3%) were MRSA and 86 (67.7%) were MSSA. All MRSA carried mecA gene whereas lukS/F-PV gene was found in 21 MRSA and 31 MSSA strains. Overall, a high antimicrobial resistance was found against MRSA and lukS/F-PV positive MSSA. Inducible clindamycin and high-level mupirocin resistance (HLMR) was 23.6% and 19.5% respectively.
A high rate of PVL toxin gene was detected among S. aureus strains and a high prevalence of antimicrobial resistant strains was observed. |
| doi_str_mv | 10.3855/jidc.9633 |
| format | Article |
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This is an analytical observational cross-section study and was conducted from July 2014 to June 2015 at four tertiary care hospitals and PCSIR Laboratories Complex, Lahore, Pakistan. A total of 376 random clinical swabs were collected from SSTI (n = 179), nasal nares (n = 134) and burn wounds (n = 63) from out-patients' departments (OPD). The specimens were cultured on nutrient and mannitol salt agar (MSA) and the organism was identified by catalase, coagulase, and DNase tests. Antimicrobial susceptibility, methicillin, inducible clindamycin, and high-level mupirocin (HLMR) resistance were determined as per CLSI guidelines. Molecular identification of mecA and lukS/F-PV genes was performed by PCR.
We isolated 127 S. aureus, where 41 (32.3%) were MRSA and 86 (67.7%) were MSSA. All MRSA carried mecA gene whereas lukS/F-PV gene was found in 21 MRSA and 31 MSSA strains. Overall, a high antimicrobial resistance was found against MRSA and lukS/F-PV positive MSSA. Inducible clindamycin and high-level mupirocin resistance (HLMR) was 23.6% and 19.5% respectively.
A high rate of PVL toxin gene was detected among S. aureus strains and a high prevalence of antimicrobial resistant strains was observed.</description><identifier>ISSN: 1972-2680</identifier><identifier>ISSN: 2036-6590</identifier><identifier>EISSN: 1972-2680</identifier><identifier>DOI: 10.3855/jidc.9633</identifier><identifier>PMID: 31999629</identifier><language>eng</language><publisher>Italy: Journal of Infection in Developing Countries</publisher><subject>Adolescent ; Adult ; Antimicrobial agents ; Bacterial Toxins - genetics ; Cross-Sectional Studies ; Drug Resistance, Bacterial - drug effects ; Drug Resistance, Bacterial - genetics ; Exotoxins - genetics ; Female ; Genes ; Hospitals, Urban ; Humans ; Leukocidins - genetics ; Male ; Methicillin-Resistant Staphylococcus aureus - genetics ; Methicillin-Resistant Staphylococcus aureus - isolation & purification ; Microbial Sensitivity Tests ; Middle Aged ; Pakistan - epidemiology ; Penicillin ; Prevalence ; Staphylococcal Infections - drug therapy ; Staphylococcal Infections - epidemiology ; Staphylococcal Infections - microbiology ; Staphylococcus aureus - drug effects ; Staphylococcus aureus - genetics ; Staphylococcus aureus - isolation & purification ; Staphylococcus infections ; Young Adult</subject><ispartof>Journal of infection in developing countries, 2018-09, Vol.12 (9), p.720-725</ispartof><rights>Copyright (c) 2018 Muhammad Shaheen Iqbal, Yasar Saleem, Farheen Ansari, Sania Mazhar, Muhammad Usman Qamar, Abida Hassan, Shaista Nawaz, Salman Saeed, Quratulain Syed.</rights><rights>2018. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c313t-2f306f5cf4e0716709fbf292cfc76b966cae64af5b1bdb56afc81d3aa16a58513</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31999629$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Iqbal, Muhammad Shaheen</creatorcontrib><creatorcontrib>Saleem, Yasar</creatorcontrib><creatorcontrib>Ansari, Farheen</creatorcontrib><creatorcontrib>Qamar, Muhammad Usman</creatorcontrib><creatorcontrib>Mazhar, Sania</creatorcontrib><creatorcontrib>Hassan, Abida</creatorcontrib><creatorcontrib>Nawaz, Shaista</creatorcontrib><creatorcontrib>Saeed, Salman</creatorcontrib><creatorcontrib>Syed, Quratulain</creatorcontrib><title>Staphylococcus aureus carrying lukS/F Panton-Valentine Leukocidin (PVL) toxin genes in hospitals of Lahore city</title><title>Journal of infection in developing countries</title><addtitle>J Infect Dev Ctries</addtitle><description>Panton Valentine-Leukocidin (PVL) toxin is secreted by Staphylococcus aureus and is mostly associated with skin and soft tissue infections (SSTI). This study aims to find out the prevalence of lukS/F-PV gene, which encode PVL toxin from strains of SSTI, burn wounds and nasal colonizers of out-patients and to measure the antimicrobial susceptibility of S. aureus isolates.
This is an analytical observational cross-section study and was conducted from July 2014 to June 2015 at four tertiary care hospitals and PCSIR Laboratories Complex, Lahore, Pakistan. A total of 376 random clinical swabs were collected from SSTI (n = 179), nasal nares (n = 134) and burn wounds (n = 63) from out-patients' departments (OPD). The specimens were cultured on nutrient and mannitol salt agar (MSA) and the organism was identified by catalase, coagulase, and DNase tests. Antimicrobial susceptibility, methicillin, inducible clindamycin, and high-level mupirocin (HLMR) resistance were determined as per CLSI guidelines. Molecular identification of mecA and lukS/F-PV genes was performed by PCR.
We isolated 127 S. aureus, where 41 (32.3%) were MRSA and 86 (67.7%) were MSSA. All MRSA carried mecA gene whereas lukS/F-PV gene was found in 21 MRSA and 31 MSSA strains. Overall, a high antimicrobial resistance was found against MRSA and lukS/F-PV positive MSSA. Inducible clindamycin and high-level mupirocin resistance (HLMR) was 23.6% and 19.5% respectively.
A high rate of PVL toxin gene was detected among S. aureus strains and a high prevalence of antimicrobial resistant strains was observed.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Antimicrobial agents</subject><subject>Bacterial Toxins - genetics</subject><subject>Cross-Sectional Studies</subject><subject>Drug Resistance, Bacterial - drug effects</subject><subject>Drug Resistance, Bacterial - genetics</subject><subject>Exotoxins - genetics</subject><subject>Female</subject><subject>Genes</subject><subject>Hospitals, Urban</subject><subject>Humans</subject><subject>Leukocidins - genetics</subject><subject>Male</subject><subject>Methicillin-Resistant Staphylococcus aureus - genetics</subject><subject>Methicillin-Resistant Staphylococcus aureus - isolation & purification</subject><subject>Microbial Sensitivity Tests</subject><subject>Middle Aged</subject><subject>Pakistan - epidemiology</subject><subject>Penicillin</subject><subject>Prevalence</subject><subject>Staphylococcal Infections - drug therapy</subject><subject>Staphylococcal Infections - epidemiology</subject><subject>Staphylococcal Infections - microbiology</subject><subject>Staphylococcus aureus - drug effects</subject><subject>Staphylococcus aureus - genetics</subject><subject>Staphylococcus aureus - isolation & purification</subject><subject>Staphylococcus infections</subject><subject>Young Adult</subject><issn>1972-2680</issn><issn>2036-6590</issn><issn>1972-2680</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNpdkUFLAzEUhIMoVqsH_4AEvLSHbZNNk26OUqwKCxaqvS7ZbNKm3SY12QX7701pFfE0w-NjGN4AcIfRgGSUDtemkgPOCDkDV5iP0yRlGTr_4zvgOoQ1QpQTii9Bh2DOOUv5FXDzRuxW-9pJJ2UboGi9iiKF93tjl7BuN_PhFM6EbZxNFqJWtjFWwVy1GydNZSzszRZ5HzbuK_qlsirAaFYu7Ewj6gCdhrlYOa-gNM3-BlzoeFW3J-2Cj-nT--Qlyd-eXyePeSIJJk2SaoKYplKPFBpjNkZclzrlqdRyzErOmBSKjYSmJS6rkjKhZYYrIgRmgmYUky7oHXN33n22KjTF1gSp6lpY5dpQpIQixGM0i-jDP3TtWm9juyKlDGE0yjCKVP9ISe9C8EoXO2-2wu8LjIrDCsVhheKwQmTvT4ltuVXVL_nzdvINR32DbQ</recordid><startdate>20180930</startdate><enddate>20180930</enddate><creator>Iqbal, Muhammad Shaheen</creator><creator>Saleem, Yasar</creator><creator>Ansari, Farheen</creator><creator>Qamar, Muhammad Usman</creator><creator>Mazhar, Sania</creator><creator>Hassan, Abida</creator><creator>Nawaz, Shaista</creator><creator>Saeed, Salman</creator><creator>Syed, Quratulain</creator><general>Journal of Infection in Developing Countries</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8C1</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20180930</creationdate><title>Staphylococcus aureus carrying lukS/F Panton-Valentine Leukocidin (PVL) toxin genes in hospitals of Lahore city</title><author>Iqbal, Muhammad Shaheen ; 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This study aims to find out the prevalence of lukS/F-PV gene, which encode PVL toxin from strains of SSTI, burn wounds and nasal colonizers of out-patients and to measure the antimicrobial susceptibility of S. aureus isolates.
This is an analytical observational cross-section study and was conducted from July 2014 to June 2015 at four tertiary care hospitals and PCSIR Laboratories Complex, Lahore, Pakistan. A total of 376 random clinical swabs were collected from SSTI (n = 179), nasal nares (n = 134) and burn wounds (n = 63) from out-patients' departments (OPD). The specimens were cultured on nutrient and mannitol salt agar (MSA) and the organism was identified by catalase, coagulase, and DNase tests. Antimicrobial susceptibility, methicillin, inducible clindamycin, and high-level mupirocin (HLMR) resistance were determined as per CLSI guidelines. Molecular identification of mecA and lukS/F-PV genes was performed by PCR.
We isolated 127 S. aureus, where 41 (32.3%) were MRSA and 86 (67.7%) were MSSA. All MRSA carried mecA gene whereas lukS/F-PV gene was found in 21 MRSA and 31 MSSA strains. Overall, a high antimicrobial resistance was found against MRSA and lukS/F-PV positive MSSA. Inducible clindamycin and high-level mupirocin resistance (HLMR) was 23.6% and 19.5% respectively.
A high rate of PVL toxin gene was detected among S. aureus strains and a high prevalence of antimicrobial resistant strains was observed.</abstract><cop>Italy</cop><pub>Journal of Infection in Developing Countries</pub><pmid>31999629</pmid><doi>10.3855/jidc.9633</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adolescent Adult Antimicrobial agents Bacterial Toxins - genetics Cross-Sectional Studies Drug Resistance, Bacterial - drug effects Drug Resistance, Bacterial - genetics Exotoxins - genetics Female Genes Hospitals, Urban Humans Leukocidins - genetics Male Methicillin-Resistant Staphylococcus aureus - genetics Methicillin-Resistant Staphylococcus aureus - isolation & purification Microbial Sensitivity Tests Middle Aged Pakistan - epidemiology Penicillin Prevalence Staphylococcal Infections - drug therapy Staphylococcal Infections - epidemiology Staphylococcal Infections - microbiology Staphylococcus aureus - drug effects Staphylococcus aureus - genetics Staphylococcus aureus - isolation & purification Staphylococcus infections Young Adult |
| title | Staphylococcus aureus carrying lukS/F Panton-Valentine Leukocidin (PVL) toxin genes in hospitals of Lahore city |
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