Obtaining active recombinant proteins from bacterial inclusion bodies using salt solutions under neutral pH conditions

Eukaryotic recombinant proteins expressed in bacterial cells usually aggregate within the cells as inclusion bodies. Despite the widely-accepted theory considering inclusion bodies as inactive materials, inclusion bodies may contain large amounts of correctly-folded active recombinant proteins. Prot...

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Veröffentlicht in:	Protein expression and purification 2020-05, Vol.169, p.105586-105586, Article 105586
Hauptverfasser:	Najafi, Marzieh, Safdari, Yaghoub
Format:	Artikel
Sprache:	eng
Schlagworte:	KNO3 MnSO4 Salt solutions scFv ZnSO4
Online-Zugang:	Volltext
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