A highly sensitive semi-nested real-time PCR utilizing oligospermine-conjugated degenerate primers for the detection of diverse strains of small ruminant lentiviruses

Small ruminant lentiviruses (SRLVs) are highly diverse retroviruses infecting sheep and goats. Although PCR-based testing is being utilized for diagnostics, its application is hampered by various factors. These include, among others, the exceptionally high genetic variability of SRLVs, as well as th...

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Veröffentlicht in:	Molecular and cellular probes 2020-06, Vol.51, p.101528-101528, Article 101528
Hauptverfasser:	Chassalevris, Taxiarchis, Chaintoutis, Serafeim C., Apostolidi, Evangelia D., Giadinis, Nektarios D., Vlemmas, Ioannis, Brellou, Georgia D., Dovas, Chrysostomos I.
Format:	Artikel
Sprache:	eng
Schlagworte:	Degenerate primers DNA extraction Proviral DNA Semi-nested real-time PCR Small ruminant lentiviruses
Online-Zugang:	Volltext
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