Methods for macrophage differentiation and in vitro generation of human tumor associated-like macrophages
Tumor-associated macrophages (TAMs) are becoming a promising target for cancer immunotherapy. Significant efforts have been made to study the detrimental role of TAMs both in vivo and in vitro. However, it remains challenging to isolate these macrophages to study their function in human cancers and...
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| Veröffentlicht in: | Methods in enzymology 2020, Vol.632, p.113-131 |
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| container_title | Methods in enzymology |
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| creator | Heideveld, Esther Horcas-Lopez, Marta Lopez-Yrigoyen, Martha Forrester, Lesley M Cassetta, Luca Pollard, Jeffrey W |
| description | Tumor-associated macrophages (TAMs) are becoming a promising target for cancer immunotherapy. Significant efforts have been made to study the detrimental role of TAMs both in vivo and in vitro. However, it remains challenging to isolate these macrophages to study their function in human cancers and there is the need to seek alternatives to address these limitations. In this review, we will focus on the three most relevant approaches to obtain in vitro fully differentiated macrophages i.e. peripheral blood, immortalized cell lines such as THP-1 or human induced pluripotent stem cells. We will also provide protocols for the polarization of human macrophages to a TAM-like cells in vitro. |
| doi_str_mv | 10.1016/bs.mie.2019.10.005 |
| format | Article |
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| identifier | EISSN: 1557-7988 |
| ispartof | Methods in enzymology, 2020, Vol.632, p.113-131 |
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| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Cell Culture Techniques - methods Cell Differentiation Cell Line Cell Separation - methods Humans Immunophenotyping - methods Induced Pluripotent Stem Cells - cytology Induced Pluripotent Stem Cells - immunology Monocytes - cytology Monocytes - immunology Tumor-Associated Macrophages - cytology Tumor-Associated Macrophages - immunology |
| title | Methods for macrophage differentiation and in vitro generation of human tumor associated-like macrophages |
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