Enzymatically elongated rice starches by amylosucrase from Deinococcus geothermalis lead to slow down the glucose generation rate at the mammalian α-glucosidase level
Amylosucrase (AS) catalyzes the transfer of a glucosyl unit from sucrose onto α-1,4-linked glucan polymers in starch. In this study, AS from Deinococcus geothermalis (DgAS) was applied to produce modified rice starches with slowly digestible properties. DgAS-treated waxy and normal rice starches sho...
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| Veröffentlicht in: | International journal of biological macromolecules 2020-04, Vol.149, p.767-772 |
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| container_title | International journal of biological macromolecules |
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| creator | Jung, Ho-Tak Park, Cheon-Seok Shim, Ye-Eun Shin, Hansol Baik, Moo-Yeol Kim, Hyun-Seok Yoo, Sang-Ho Seo, Dong-Ho Lee, Byung-Hoo |
| description | Amylosucrase (AS) catalyzes the transfer of a glucosyl unit from sucrose onto α-1,4-linked glucan polymers in starch. In this study, AS from Deinococcus geothermalis (DgAS) was applied to produce modified rice starches with slowly digestible properties. DgAS-treated waxy and normal rice starches showed significantly (p |
| doi_str_mv | 10.1016/j.ijbiomac.2020.01.266 |
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•DgAS treatment elongated α-glucan chains in rice starches.•Slowly digestible starch contents in rice starch were increased by DgAS.•The glucose generation rate is lower for DgAS-treated than for native starch.•DgAS can be applied to functional foods for the regulation of blood glucose levels.</description><identifier>ISSN: 0141-8130</identifier><identifier>EISSN: 1879-0003</identifier><identifier>DOI: 10.1016/j.ijbiomac.2020.01.266</identifier><identifier>PMID: 32001286</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>alpha-Glucosidases - metabolism ; Amylosucrase from Deinococcus geothermalis ; Animals ; Blood Glucose ; Deinococcus - enzymology ; Glucose - metabolism ; Glucosyltransferases - metabolism ; Mammals ; Neisseria ; Oryza - chemistry ; Power, Psychological ; Rice starch ; Slowly digestible starch ; Solubility ; Starch - chemistry ; Starch - metabolism ; Water - chemistry</subject><ispartof>International journal of biological macromolecules, 2020-04, Vol.149, p.767-772</ispartof><rights>2020 Elsevier B.V.</rights><rights>Copyright © 2020 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c368t-a4070cdd7a9ef8ee197c2d73e8dcad6ac943ba8390771ee55e7cd9a7661ce4e93</citedby><cites>FETCH-LOGICAL-c368t-a4070cdd7a9ef8ee197c2d73e8dcad6ac943ba8390771ee55e7cd9a7661ce4e93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0141813019329563$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32001286$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jung, Ho-Tak</creatorcontrib><creatorcontrib>Park, Cheon-Seok</creatorcontrib><creatorcontrib>Shim, Ye-Eun</creatorcontrib><creatorcontrib>Shin, Hansol</creatorcontrib><creatorcontrib>Baik, Moo-Yeol</creatorcontrib><creatorcontrib>Kim, Hyun-Seok</creatorcontrib><creatorcontrib>Yoo, Sang-Ho</creatorcontrib><creatorcontrib>Seo, Dong-Ho</creatorcontrib><creatorcontrib>Lee, Byung-Hoo</creatorcontrib><title>Enzymatically elongated rice starches by amylosucrase from Deinococcus geothermalis lead to slow down the glucose generation rate at the mammalian α-glucosidase level</title><title>International journal of biological macromolecules</title><addtitle>Int J Biol Macromol</addtitle><description>Amylosucrase (AS) catalyzes the transfer of a glucosyl unit from sucrose onto α-1,4-linked glucan polymers in starch. In this study, AS from Deinococcus geothermalis (DgAS) was applied to produce modified rice starches with slowly digestible properties. DgAS-treated waxy and normal rice starches showed significantly (p < 0.05) elevated degrees of polymerization, suggesting that the external chains were elongated. Additionally, the crystalline structures of starches changed from A- to B-type, and the temperature transition properties of enzymatically modified rice starches increased. The amounts of slowly digestible starch (SDS) increased remarkably (20.1% and 18.8%; waxy and normal rice starches, respectively), and the DgAS-treated rice starches were slowly hydrolyzed to glucose at the mammalian mucosal α-glucosidase level. Thus, DgAS-treated rice starches can be used to produce SDS-based ingredients that attenuate the glucose spike after glycemic food ingestion.
•DgAS treatment elongated α-glucan chains in rice starches.•Slowly digestible starch contents in rice starch were increased by DgAS.•The glucose generation rate is lower for DgAS-treated than for native starch.•DgAS can be applied to functional foods for the regulation of blood glucose levels.</description><subject>alpha-Glucosidases - metabolism</subject><subject>Amylosucrase from Deinococcus geothermalis</subject><subject>Animals</subject><subject>Blood Glucose</subject><subject>Deinococcus - enzymology</subject><subject>Glucose - metabolism</subject><subject>Glucosyltransferases - metabolism</subject><subject>Mammals</subject><subject>Neisseria</subject><subject>Oryza - chemistry</subject><subject>Power, Psychological</subject><subject>Rice starch</subject><subject>Slowly digestible starch</subject><subject>Solubility</subject><subject>Starch - chemistry</subject><subject>Starch - metabolism</subject><subject>Water - chemistry</subject><issn>0141-8130</issn><issn>1879-0003</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1uFDEQRlsIRIbAFSIv2XRjt2ds9w4Uwo8UiQ2srZpyzcQjux3s7kTNhVhzEc6Eh0nYsirJ9T5_Kr2muRC8E1yoN4fOH7Y-RcCu5z3vuOh6pZ40K2H00HLO5dNmxcVatEZIfta8KOVQX9VGmOfNmew5F71Rq-bn1fhjiTB5hBAWRiGNe5jIseyRWJkg4w0Vtl0YxCWkMmOGQmyXU2TvyY8JE-Jc2J7SdEM5QvCFBQLHpsRKSPfMpfuR1R3bhxlTze5ppFwb08jqIAbT33WEeEzDyH7_ak-sd8euQHcUXjbPdhAKvXqY5823D1dfLz-1118-fr58d92iVGZqYc01R-c0DLQzRGLQ2DstyTgEpwCHtdyCkQPXWhBtNqTRDaCVEkhrGuR58_r0721O32cqk42-IIUAI6W52F5uODdGG1lRdUIxp1Iy7ext9hHyYgW3R0n2YB8l2aMky4Wtkmrw4qFj3kZy_2KPVirw9gRQvfTOU7YFPY1IzmfCybrk_9fxB0dLrKo</recordid><startdate>20200415</startdate><enddate>20200415</enddate><creator>Jung, Ho-Tak</creator><creator>Park, Cheon-Seok</creator><creator>Shim, Ye-Eun</creator><creator>Shin, Hansol</creator><creator>Baik, Moo-Yeol</creator><creator>Kim, Hyun-Seok</creator><creator>Yoo, Sang-Ho</creator><creator>Seo, Dong-Ho</creator><creator>Lee, Byung-Hoo</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20200415</creationdate><title>Enzymatically elongated rice starches by amylosucrase from Deinococcus geothermalis lead to slow down the glucose generation rate at the mammalian α-glucosidase level</title><author>Jung, Ho-Tak ; Park, Cheon-Seok ; Shim, Ye-Eun ; Shin, Hansol ; Baik, Moo-Yeol ; Kim, Hyun-Seok ; Yoo, Sang-Ho ; Seo, Dong-Ho ; Lee, Byung-Hoo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c368t-a4070cdd7a9ef8ee197c2d73e8dcad6ac943ba8390771ee55e7cd9a7661ce4e93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>alpha-Glucosidases - metabolism</topic><topic>Amylosucrase from Deinococcus geothermalis</topic><topic>Animals</topic><topic>Blood Glucose</topic><topic>Deinococcus - enzymology</topic><topic>Glucose - metabolism</topic><topic>Glucosyltransferases - metabolism</topic><topic>Mammals</topic><topic>Neisseria</topic><topic>Oryza - chemistry</topic><topic>Power, Psychological</topic><topic>Rice starch</topic><topic>Slowly digestible starch</topic><topic>Solubility</topic><topic>Starch - chemistry</topic><topic>Starch - metabolism</topic><topic>Water - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jung, Ho-Tak</creatorcontrib><creatorcontrib>Park, Cheon-Seok</creatorcontrib><creatorcontrib>Shim, Ye-Eun</creatorcontrib><creatorcontrib>Shin, Hansol</creatorcontrib><creatorcontrib>Baik, Moo-Yeol</creatorcontrib><creatorcontrib>Kim, Hyun-Seok</creatorcontrib><creatorcontrib>Yoo, Sang-Ho</creatorcontrib><creatorcontrib>Seo, Dong-Ho</creatorcontrib><creatorcontrib>Lee, Byung-Hoo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of biological macromolecules</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jung, Ho-Tak</au><au>Park, Cheon-Seok</au><au>Shim, Ye-Eun</au><au>Shin, Hansol</au><au>Baik, Moo-Yeol</au><au>Kim, Hyun-Seok</au><au>Yoo, Sang-Ho</au><au>Seo, Dong-Ho</au><au>Lee, Byung-Hoo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enzymatically elongated rice starches by amylosucrase from Deinococcus geothermalis lead to slow down the glucose generation rate at the mammalian α-glucosidase level</atitle><jtitle>International journal of biological macromolecules</jtitle><addtitle>Int J Biol Macromol</addtitle><date>2020-04-15</date><risdate>2020</risdate><volume>149</volume><spage>767</spage><epage>772</epage><pages>767-772</pages><issn>0141-8130</issn><eissn>1879-0003</eissn><abstract>Amylosucrase (AS) catalyzes the transfer of a glucosyl unit from sucrose onto α-1,4-linked glucan polymers in starch. In this study, AS from Deinococcus geothermalis (DgAS) was applied to produce modified rice starches with slowly digestible properties. DgAS-treated waxy and normal rice starches showed significantly (p < 0.05) elevated degrees of polymerization, suggesting that the external chains were elongated. Additionally, the crystalline structures of starches changed from A- to B-type, and the temperature transition properties of enzymatically modified rice starches increased. The amounts of slowly digestible starch (SDS) increased remarkably (20.1% and 18.8%; waxy and normal rice starches, respectively), and the DgAS-treated rice starches were slowly hydrolyzed to glucose at the mammalian mucosal α-glucosidase level. Thus, DgAS-treated rice starches can be used to produce SDS-based ingredients that attenuate the glucose spike after glycemic food ingestion.
•DgAS treatment elongated α-glucan chains in rice starches.•Slowly digestible starch contents in rice starch were increased by DgAS.•The glucose generation rate is lower for DgAS-treated than for native starch.•DgAS can be applied to functional foods for the regulation of blood glucose levels.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>32001286</pmid><doi>10.1016/j.ijbiomac.2020.01.266</doi><tpages>6</tpages></addata></record> |
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| subjects | alpha-Glucosidases - metabolism Amylosucrase from Deinococcus geothermalis Animals Blood Glucose Deinococcus - enzymology Glucose - metabolism Glucosyltransferases - metabolism Mammals Neisseria Oryza - chemistry Power, Psychological Rice starch Slowly digestible starch Solubility Starch - chemistry Starch - metabolism Water - chemistry |
| title | Enzymatically elongated rice starches by amylosucrase from Deinococcus geothermalis lead to slow down the glucose generation rate at the mammalian α-glucosidase level |
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