Dental Caries, Developmental Defects of Enamel and Enamel Microhardness Associated with Genetic Polymorphisms in the RANK/RANKL/OPG System
: Recent studies have suggested that disruptions in the RANKL/RANK/OPG system might be involved in enamel conditions. The aim of this study was to test whether genetic polymorphisms in RANK, RANKL and OPG are associated with dental caries, developmental defects of enamel (DDE) and enamel microhardne...
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| Veröffentlicht in: | The Journal of clinical pediatric dentistry 2020, Vol.44 (1), p.35-40 |
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| creator | Calvano Küchler, E Maschietto Pucinelli, C Carpio Horta, K Assed Bezerra da Silva, R de Castro Costa, M Rezende Vieira, A Nelson-Filho, P Assed Bezerra da Silva, L Santos Antunes, L Azeredo Antunes, L |
| description | : Recent studies have suggested that disruptions in the RANKL/RANK/OPG system might be involved in enamel conditions. The aim of this study was to test whether genetic polymorphisms in RANK, RANKL and OPG are associated with dental caries, developmental defects of enamel (DDE) and enamel microhardness.
: Saliva samples were collected from two subsets for the purpose of genomic DNA extraction. In the first subset, composed of 248 children, dental caries and DDE were evaluated during their clinical examination. In the second subset, composed of 72 children, enamel samples from the buccal surface of primary teeth were used for enamel microhardness analysis. Genetic polymorphisms in RANK, RANKL and OPG were genotyped by real-time polymerase chain reactions in all samples from both populations. The chi-square test was used for dental caries and DDE analysis while, one-way ANOVA with Tukey's post-test was used for microhardness analysis. Hardy-Weinberg equilibrium was also calculated. The established alpha was 5%.
Caries experience analysis demonstrated a statistically-significant difference for OPG allele distribution in primary dentition (p=0.033). The studied polymorphisms in RANK, RANKL and OPG were not associated with DDE or enamel microhardness (p>0.05).
The genetic polymorphism rs2073618 in OPG is associated with dental caries experience in primary dentition. |
| doi_str_mv | 10.17796/1053-4625-44.1.6 |
| format | Article |
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: Saliva samples were collected from two subsets for the purpose of genomic DNA extraction. In the first subset, composed of 248 children, dental caries and DDE were evaluated during their clinical examination. In the second subset, composed of 72 children, enamel samples from the buccal surface of primary teeth were used for enamel microhardness analysis. Genetic polymorphisms in RANK, RANKL and OPG were genotyped by real-time polymerase chain reactions in all samples from both populations. The chi-square test was used for dental caries and DDE analysis while, one-way ANOVA with Tukey's post-test was used for microhardness analysis. Hardy-Weinberg equilibrium was also calculated. The established alpha was 5%.
Caries experience analysis demonstrated a statistically-significant difference for OPG allele distribution in primary dentition (p=0.033). The studied polymorphisms in RANK, RANKL and OPG were not associated with DDE or enamel microhardness (p>0.05).
The genetic polymorphism rs2073618 in OPG is associated with dental caries experience in primary dentition.</description><identifier>ISSN: 1053-4628</identifier><identifier>EISSN: 1557-5268</identifier><identifier>DOI: 10.17796/1053-4625-44.1.6</identifier><identifier>PMID: 31995419</identifier><language>eng</language><publisher>United States: Journal of Clinical Pediatric Dentistry</publisher><subject>Child ; Defects ; Dental Caries ; Dental Enamel ; Dental Enamel Hypoplasia ; Dentistry ; Deoxyribonucleic acid ; DNA ; Homeostasis ; Humans ; Mineralization ; Pediatrics ; Polymorphism ; Polymorphism, Genetic ; Teeth ; Tooth, Deciduous ; Tumor necrosis factor-TNF</subject><ispartof>The Journal of clinical pediatric dentistry, 2020, Vol.44 (1), p.35-40</ispartof><rights>Copyright Journal of Clinical Pediatric Dentistry 2020</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c329t-cc23b8a161eba3691bae13822add411f6b759e823b8f3173a3cd448498bc13a53</citedby><cites>FETCH-LOGICAL-c329t-cc23b8a161eba3691bae13822add411f6b759e823b8f3173a3cd448498bc13a53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,4010,27904,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31995419$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Calvano Küchler, E</creatorcontrib><creatorcontrib>Maschietto Pucinelli, C</creatorcontrib><creatorcontrib>Carpio Horta, K</creatorcontrib><creatorcontrib>Assed Bezerra da Silva, R</creatorcontrib><creatorcontrib>de Castro Costa, M</creatorcontrib><creatorcontrib>Rezende Vieira, A</creatorcontrib><creatorcontrib>Nelson-Filho, P</creatorcontrib><creatorcontrib>Assed Bezerra da Silva, L</creatorcontrib><creatorcontrib>Santos Antunes, L</creatorcontrib><creatorcontrib>Azeredo Antunes, L</creatorcontrib><title>Dental Caries, Developmental Defects of Enamel and Enamel Microhardness Associated with Genetic Polymorphisms in the RANK/RANKL/OPG System</title><title>The Journal of clinical pediatric dentistry</title><addtitle>J Clin Pediatr Dent</addtitle><description>: Recent studies have suggested that disruptions in the RANKL/RANK/OPG system might be involved in enamel conditions. The aim of this study was to test whether genetic polymorphisms in RANK, RANKL and OPG are associated with dental caries, developmental defects of enamel (DDE) and enamel microhardness.
: Saliva samples were collected from two subsets for the purpose of genomic DNA extraction. In the first subset, composed of 248 children, dental caries and DDE were evaluated during their clinical examination. In the second subset, composed of 72 children, enamel samples from the buccal surface of primary teeth were used for enamel microhardness analysis. Genetic polymorphisms in RANK, RANKL and OPG were genotyped by real-time polymerase chain reactions in all samples from both populations. The chi-square test was used for dental caries and DDE analysis while, one-way ANOVA with Tukey's post-test was used for microhardness analysis. Hardy-Weinberg equilibrium was also calculated. The established alpha was 5%.
Caries experience analysis demonstrated a statistically-significant difference for OPG allele distribution in primary dentition (p=0.033). The studied polymorphisms in RANK, RANKL and OPG were not associated with DDE or enamel microhardness (p>0.05).
The genetic polymorphism rs2073618 in OPG is associated with dental caries experience in primary dentition.</description><subject>Child</subject><subject>Defects</subject><subject>Dental Caries</subject><subject>Dental Enamel</subject><subject>Dental Enamel Hypoplasia</subject><subject>Dentistry</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Homeostasis</subject><subject>Humans</subject><subject>Mineralization</subject><subject>Pediatrics</subject><subject>Polymorphism</subject><subject>Polymorphism, Genetic</subject><subject>Teeth</subject><subject>Tooth, Deciduous</subject><subject>Tumor necrosis factor-TNF</subject><issn>1053-4628</issn><issn>1557-5268</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNpdkc9u2zAMxoVhw9p1e4BdBgG77FAnoiTb8jFI2qxYthb7cxZkmUZU2FYmKS3yCnvq2U26wy4kQf74geBHyHtgMyjLqpgDy0UmC55nUs5gVrwg55DnZZbzQr0c69NYnZE3Md4zxlSh2GtyJqCqcgnVOfmzwiGZji5NcBgv6QofsPO7_thdYYs2RepbejWYHjtqhua5_Ops8FsTmgFjpIsYvXUmYUMfXdrSNQ6YnKV3vjv0Puy2LvaRuoGmLdLvi29f5lPYzG_v1vTHISbs35JXrekivjvlC_Lr-urn8nO2uV3fLBebzApepcxaLmploACsjSgqqA2CUJybppEAbVGXeYVqgloBpTDCNlIqWanagjC5uCCfjrq74H_vMSbdu2ix68yAfh81F1IpBkywEf34H3rv92EYr9NcclBKsnKi4EiN_4gxYKt3wfUmHDQw_WSUnpzQk1FaSg26GHc-nJT3dY_Nv41nZ8RfI6WNUA</recordid><startdate>2020</startdate><enddate>2020</enddate><creator>Calvano Küchler, E</creator><creator>Maschietto Pucinelli, C</creator><creator>Carpio Horta, K</creator><creator>Assed Bezerra da Silva, R</creator><creator>de Castro Costa, M</creator><creator>Rezende Vieira, A</creator><creator>Nelson-Filho, P</creator><creator>Assed Bezerra da Silva, L</creator><creator>Santos Antunes, L</creator><creator>Azeredo Antunes, L</creator><general>Journal of Clinical Pediatric Dentistry</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>2020</creationdate><title>Dental Caries, Developmental Defects of Enamel and Enamel Microhardness Associated with Genetic Polymorphisms in the RANK/RANKL/OPG System</title><author>Calvano Küchler, E ; Maschietto Pucinelli, C ; Carpio Horta, K ; Assed Bezerra da Silva, R ; de Castro Costa, M ; Rezende Vieira, A ; Nelson-Filho, P ; Assed Bezerra da Silva, L ; Santos Antunes, L ; Azeredo Antunes, L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c329t-cc23b8a161eba3691bae13822add411f6b759e823b8f3173a3cd448498bc13a53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Child</topic><topic>Defects</topic><topic>Dental Caries</topic><topic>Dental Enamel</topic><topic>Dental Enamel Hypoplasia</topic><topic>Dentistry</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Homeostasis</topic><topic>Humans</topic><topic>Mineralization</topic><topic>Pediatrics</topic><topic>Polymorphism</topic><topic>Polymorphism, Genetic</topic><topic>Teeth</topic><topic>Tooth, Deciduous</topic><topic>Tumor necrosis factor-TNF</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Calvano Küchler, E</creatorcontrib><creatorcontrib>Maschietto Pucinelli, C</creatorcontrib><creatorcontrib>Carpio Horta, K</creatorcontrib><creatorcontrib>Assed Bezerra da Silva, R</creatorcontrib><creatorcontrib>de Castro Costa, M</creatorcontrib><creatorcontrib>Rezende Vieira, A</creatorcontrib><creatorcontrib>Nelson-Filho, P</creatorcontrib><creatorcontrib>Assed Bezerra da Silva, L</creatorcontrib><creatorcontrib>Santos Antunes, L</creatorcontrib><creatorcontrib>Azeredo Antunes, L</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of clinical pediatric dentistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Calvano Küchler, E</au><au>Maschietto Pucinelli, C</au><au>Carpio Horta, K</au><au>Assed Bezerra da Silva, R</au><au>de Castro Costa, M</au><au>Rezende Vieira, A</au><au>Nelson-Filho, P</au><au>Assed Bezerra da Silva, L</au><au>Santos Antunes, L</au><au>Azeredo Antunes, L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dental Caries, Developmental Defects of Enamel and Enamel Microhardness Associated with Genetic Polymorphisms in the RANK/RANKL/OPG System</atitle><jtitle>The Journal of clinical pediatric dentistry</jtitle><addtitle>J Clin Pediatr Dent</addtitle><date>2020</date><risdate>2020</risdate><volume>44</volume><issue>1</issue><spage>35</spage><epage>40</epage><pages>35-40</pages><issn>1053-4628</issn><eissn>1557-5268</eissn><abstract>: Recent studies have suggested that disruptions in the RANKL/RANK/OPG system might be involved in enamel conditions. The aim of this study was to test whether genetic polymorphisms in RANK, RANKL and OPG are associated with dental caries, developmental defects of enamel (DDE) and enamel microhardness.
: Saliva samples were collected from two subsets for the purpose of genomic DNA extraction. In the first subset, composed of 248 children, dental caries and DDE were evaluated during their clinical examination. In the second subset, composed of 72 children, enamel samples from the buccal surface of primary teeth were used for enamel microhardness analysis. Genetic polymorphisms in RANK, RANKL and OPG were genotyped by real-time polymerase chain reactions in all samples from both populations. The chi-square test was used for dental caries and DDE analysis while, one-way ANOVA with Tukey's post-test was used for microhardness analysis. Hardy-Weinberg equilibrium was also calculated. The established alpha was 5%.
Caries experience analysis demonstrated a statistically-significant difference for OPG allele distribution in primary dentition (p=0.033). The studied polymorphisms in RANK, RANKL and OPG were not associated with DDE or enamel microhardness (p>0.05).
The genetic polymorphism rs2073618 in OPG is associated with dental caries experience in primary dentition.</abstract><cop>United States</cop><pub>Journal of Clinical Pediatric Dentistry</pub><pmid>31995419</pmid><doi>10.17796/1053-4625-44.1.6</doi><tpages>6</tpages></addata></record> |
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| ispartof | The Journal of clinical pediatric dentistry, 2020, Vol.44 (1), p.35-40 |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Child Defects Dental Caries Dental Enamel Dental Enamel Hypoplasia Dentistry Deoxyribonucleic acid DNA Homeostasis Humans Mineralization Pediatrics Polymorphism Polymorphism, Genetic Teeth Tooth, Deciduous Tumor necrosis factor-TNF |
| title | Dental Caries, Developmental Defects of Enamel and Enamel Microhardness Associated with Genetic Polymorphisms in the RANK/RANKL/OPG System |
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