Statistical Optimization for Coproduction of Chitinase and Beta 1, 4-Endoglucanase by Chitinolytic Paenibacillus elgii PB1 Having Antifungal Activity
A bacterial strain PB1 with antagonistic activity against pathogenic fungi was isolated from marine soil and was identified as Paenibacillus elgii based on phenotypic and genotypic characterization. The isolate showed good antifungal activity against “ Aspergillus niger (MTCC 282), Trichophyton rubr...
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| Veröffentlicht in: | Applied biochemistry and biotechnology 2020-05, Vol.191 (1), p.135-150 |
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| creator | Philip, Nancy V. Koteshwara, Ananthamurthy Kiran, G. Aditya Raja, S. Subrahmanyam, V. M. Chandrashekar, H. Raghu |
| description | A bacterial strain PB1 with antagonistic activity against pathogenic fungi was isolated from marine soil and was identified as
Paenibacillus elgii
based on phenotypic and genotypic characterization. The isolate showed good antifungal activity against “
Aspergillus niger
(MTCC 282),
Trichophyton rubrum
(MTCC 791),
Microsporum gypseum
(MTCC 2819),
Candida albicans
(MTCC 227), and
Saccharomyces cerevisiae
(MTCC 170)”. Chitinase and beta 1, 4-endoglucanase are known for their capability to degrade fungal cell wall, thus we analyzed its productivity in PB1 strain using Plackett-Burman and Central Composite Design. The factors that affect the productivity of chitinase and beta 1, 4-endoglucanase were identified and optimized. A 7.77-fold increase (3.157 to 24.53 ± 1.33 U/mL) in chitinase and 7.422-fold increase (6.476 to 48.066 ± 0.676 U/mL) in beta 1, 4-endoglucanase versus basal medium was achieved. Chitinase and beta 1, 4-endoglucanase produced by
Paenibacillus elgii
strain PB1 represents the new source for biotechnological, medical, and agricultural applications. |
| doi_str_mv | 10.1007/s12010-020-03235-8 |
| format | Article |
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Paenibacillus elgii
based on phenotypic and genotypic characterization. The isolate showed good antifungal activity against “
Aspergillus niger
(MTCC 282),
Trichophyton rubrum
(MTCC 791),
Microsporum gypseum
(MTCC 2819),
Candida albicans
(MTCC 227), and
Saccharomyces cerevisiae
(MTCC 170)”. Chitinase and beta 1, 4-endoglucanase are known for their capability to degrade fungal cell wall, thus we analyzed its productivity in PB1 strain using Plackett-Burman and Central Composite Design. The factors that affect the productivity of chitinase and beta 1, 4-endoglucanase were identified and optimized. A 7.77-fold increase (3.157 to 24.53 ± 1.33 U/mL) in chitinase and 7.422-fold increase (6.476 to 48.066 ± 0.676 U/mL) in beta 1, 4-endoglucanase versus basal medium was achieved. Chitinase and beta 1, 4-endoglucanase produced by
Paenibacillus elgii
strain PB1 represents the new source for biotechnological, medical, and agricultural applications.</description><identifier>ISSN: 0273-2289</identifier><identifier>EISSN: 1559-0291</identifier><identifier>DOI: 10.1007/s12010-020-03235-8</identifier><identifier>PMID: 31989438</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Antifungal activity ; Antifungal agents ; Antifungal Agents - chemistry ; Antifungal Agents - isolation & purification ; Antifungal Agents - pharmacology ; Aquatic soils ; Bacterial Proteins - biosynthesis ; Bacterial Proteins - chemistry ; Bacterial Proteins - isolation & purification ; Bacterial Proteins - pharmacology ; Biochemistry ; Biotechnology ; Cell walls ; Chemistry ; Chemistry and Materials Science ; Chitinase ; Chitinases - biosynthesis ; Chitinases - chemistry ; Chitinases - isolation & purification ; Chitinases - pharmacology ; Design factors ; Endoglucanase ; Fungi ; Fungi - growth & development ; Fungicides ; Optimization ; Paenibacillus ; Paenibacillus - enzymology ; Yeast</subject><ispartof>Applied biochemistry and biotechnology, 2020-05, Vol.191 (1), p.135-150</ispartof><rights>Springer Science+Business Media, LLC, part of Springer Nature 2020</rights><rights>Springer Science+Business Media, LLC, part of Springer Nature 2020.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c412t-39d38d9e3f03bb850072dad5a7fcbb22d8867d5556bcbfdc948b4372d003c9c3</citedby><cites>FETCH-LOGICAL-c412t-39d38d9e3f03bb850072dad5a7fcbb22d8867d5556bcbfdc948b4372d003c9c3</cites><orcidid>0000-0002-6538-7758</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12010-020-03235-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12010-020-03235-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31989438$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Philip, Nancy V.</creatorcontrib><creatorcontrib>Koteshwara, Ananthamurthy</creatorcontrib><creatorcontrib>Kiran, G. Aditya</creatorcontrib><creatorcontrib>Raja, S.</creatorcontrib><creatorcontrib>Subrahmanyam, V. M.</creatorcontrib><creatorcontrib>Chandrashekar, H. Raghu</creatorcontrib><title>Statistical Optimization for Coproduction of Chitinase and Beta 1, 4-Endoglucanase by Chitinolytic Paenibacillus elgii PB1 Having Antifungal Activity</title><title>Applied biochemistry and biotechnology</title><addtitle>Appl Biochem Biotechnol</addtitle><addtitle>Appl Biochem Biotechnol</addtitle><description>A bacterial strain PB1 with antagonistic activity against pathogenic fungi was isolated from marine soil and was identified as
Paenibacillus elgii
based on phenotypic and genotypic characterization. The isolate showed good antifungal activity against “
Aspergillus niger
(MTCC 282),
Trichophyton rubrum
(MTCC 791),
Microsporum gypseum
(MTCC 2819),
Candida albicans
(MTCC 227), and
Saccharomyces cerevisiae
(MTCC 170)”. Chitinase and beta 1, 4-endoglucanase are known for their capability to degrade fungal cell wall, thus we analyzed its productivity in PB1 strain using Plackett-Burman and Central Composite Design. The factors that affect the productivity of chitinase and beta 1, 4-endoglucanase were identified and optimized. A 7.77-fold increase (3.157 to 24.53 ± 1.33 U/mL) in chitinase and 7.422-fold increase (6.476 to 48.066 ± 0.676 U/mL) in beta 1, 4-endoglucanase versus basal medium was achieved. Chitinase and beta 1, 4-endoglucanase produced by
Paenibacillus elgii
strain PB1 represents the new source for biotechnological, medical, and agricultural applications.</description><subject>Antifungal activity</subject><subject>Antifungal agents</subject><subject>Antifungal Agents - chemistry</subject><subject>Antifungal Agents - isolation & purification</subject><subject>Antifungal Agents - pharmacology</subject><subject>Aquatic soils</subject><subject>Bacterial Proteins - biosynthesis</subject><subject>Bacterial Proteins - chemistry</subject><subject>Bacterial Proteins - isolation & purification</subject><subject>Bacterial Proteins - pharmacology</subject><subject>Biochemistry</subject><subject>Biotechnology</subject><subject>Cell walls</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Chitinase</subject><subject>Chitinases - biosynthesis</subject><subject>Chitinases - chemistry</subject><subject>Chitinases - isolation & purification</subject><subject>Chitinases - pharmacology</subject><subject>Design factors</subject><subject>Endoglucanase</subject><subject>Fungi</subject><subject>Fungi - growth & development</subject><subject>Fungicides</subject><subject>Optimization</subject><subject>Paenibacillus</subject><subject>Paenibacillus - enzymology</subject><subject>Yeast</subject><issn>0273-2289</issn><issn>1559-0291</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kc1OGzEUha2qqKS0L8ACWeqmiw71zzhjL0MEBQkJpLK3_DdTI8cOYw9SeI--LyZJqcSChWXp3O-ee-0DwDFGpxih7mfGBGHUIFIPJZQ1_AOYYcZElQT-CGaIdLQhhItD8Dnne4Qw4az7BA4pFly0lM_A399FFZ-LNyrAm3XxK_9UhRRhn0a4TOsx2clshdTD5R9ffFTZQRUtPHNFQfwDts15tGkIk1Hbmt7swRQ21RjeKhe9VsaHMGXowuA9vD3D8FI9-jjARSy-n-JQF1jUSY--bL6Ag16F7L7u7yNwd3F-t7xsrm9-XS0X141pMSkNFZZyKxztEdWas_opxCrLVNcbrQmxnM87yxiba6N7a0TLdUsrgxA1wtAj8H1nW1_5MLlc5Mpn40JQ0aUpS0LbjpF5J0hFv71B79M0xrqcJC1GggpE20qRHWXGlPPoerke_UqNG4mRfMlM7jKTNTO5zUzy2nSyt570ytnXln8hVYDugFxLcXDj_9nv2D4DjwyjCw</recordid><startdate>20200501</startdate><enddate>20200501</enddate><creator>Philip, Nancy V.</creator><creator>Koteshwara, Ananthamurthy</creator><creator>Kiran, G. 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Aditya</au><au>Raja, S.</au><au>Subrahmanyam, V. M.</au><au>Chandrashekar, H. Raghu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Statistical Optimization for Coproduction of Chitinase and Beta 1, 4-Endoglucanase by Chitinolytic Paenibacillus elgii PB1 Having Antifungal Activity</atitle><jtitle>Applied biochemistry and biotechnology</jtitle><stitle>Appl Biochem Biotechnol</stitle><addtitle>Appl Biochem Biotechnol</addtitle><date>2020-05-01</date><risdate>2020</risdate><volume>191</volume><issue>1</issue><spage>135</spage><epage>150</epage><pages>135-150</pages><issn>0273-2289</issn><eissn>1559-0291</eissn><abstract>A bacterial strain PB1 with antagonistic activity against pathogenic fungi was isolated from marine soil and was identified as
Paenibacillus elgii
based on phenotypic and genotypic characterization. The isolate showed good antifungal activity against “
Aspergillus niger
(MTCC 282),
Trichophyton rubrum
(MTCC 791),
Microsporum gypseum
(MTCC 2819),
Candida albicans
(MTCC 227), and
Saccharomyces cerevisiae
(MTCC 170)”. Chitinase and beta 1, 4-endoglucanase are known for their capability to degrade fungal cell wall, thus we analyzed its productivity in PB1 strain using Plackett-Burman and Central Composite Design. The factors that affect the productivity of chitinase and beta 1, 4-endoglucanase were identified and optimized. A 7.77-fold increase (3.157 to 24.53 ± 1.33 U/mL) in chitinase and 7.422-fold increase (6.476 to 48.066 ± 0.676 U/mL) in beta 1, 4-endoglucanase versus basal medium was achieved. Chitinase and beta 1, 4-endoglucanase produced by
Paenibacillus elgii
strain PB1 represents the new source for biotechnological, medical, and agricultural applications.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>31989438</pmid><doi>10.1007/s12010-020-03235-8</doi><tpages>16</tpages><orcidid>https://orcid.org/0000-0002-6538-7758</orcidid></addata></record> |
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| source | MEDLINE; SpringerNature Complete Journals |
| subjects | Antifungal activity Antifungal agents Antifungal Agents - chemistry Antifungal Agents - isolation & purification Antifungal Agents - pharmacology Aquatic soils Bacterial Proteins - biosynthesis Bacterial Proteins - chemistry Bacterial Proteins - isolation & purification Bacterial Proteins - pharmacology Biochemistry Biotechnology Cell walls Chemistry Chemistry and Materials Science Chitinase Chitinases - biosynthesis Chitinases - chemistry Chitinases - isolation & purification Chitinases - pharmacology Design factors Endoglucanase Fungi Fungi - growth & development Fungicides Optimization Paenibacillus Paenibacillus - enzymology Yeast |
| title | Statistical Optimization for Coproduction of Chitinase and Beta 1, 4-Endoglucanase by Chitinolytic Paenibacillus elgii PB1 Having Antifungal Activity |
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