Microencapsulated plasmids expressing Gn and Gc glycoproteins of Rift Valley Fever virus enhance humoral immune response in mice
Objectives The aim of the current study was to develop biodegradable alginate (ALG)/poly- l -lysine (PLL) microcapsules (MC) with entrapped plasmids expressing Gn and Gc glycoproteins of Rift Valley Fever virus (RVFV) and to evaluate the humoral immune response in mice. Results Expressing phRVF/Gn a...
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| Veröffentlicht in: | Biotechnology letters 2020-04, Vol.42 (4), p.529-536 |
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| creator | Selina, Oxana Imatdinov, Ilnaz Balysheva, Vera Akasov, Roman Kryukov, Alexander Balyshev, Vladimir Markvicheva, Elena |
| description | Objectives
The aim of the current study was to develop biodegradable alginate (ALG)/poly-
l
-lysine (PLL) microcapsules (MC) with entrapped plasmids expressing Gn and Gc glycoproteins of Rift Valley Fever virus (RVFV) and to evaluate the humoral immune response in mice.
Results
Expressing phRVF/Gn and phRVF/Gc plasmids which encode full-sized Gn and Gc glycoproteins and contain signal fusion protein F sequences of human parainfluenza (HPIV-1) were constructed. To protect the plasmids from cleavage by extracellular nucleases, they were entrapped into multilayer ALG/PLL microcapsules by layer-by-layer technique. To study the efficacy of humoral immune response, both native and microencapsulated plasmids were injected intramuscular into BALB/c mice. The humoral response in the mice immunized with free plasmids was characterized by virus-neutralizing antibody induction (with titres 1:4 to 1:8), while the injection of microencapsulated plasmids allowed to increase the titre level (from 1:16 to 1:32).
Conclusion
The plasmids microencapsulated in biodegradable MC could be promising for development of DNA vaccines against RVFV. |
| doi_str_mv | 10.1007/s10529-020-02816-1 |
| format | Article |
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The aim of the current study was to develop biodegradable alginate (ALG)/poly-
l
-lysine (PLL) microcapsules (MC) with entrapped plasmids expressing Gn and Gc glycoproteins of Rift Valley Fever virus (RVFV) and to evaluate the humoral immune response in mice.
Results
Expressing phRVF/Gn and phRVF/Gc plasmids which encode full-sized Gn and Gc glycoproteins and contain signal fusion protein F sequences of human parainfluenza (HPIV-1) were constructed. To protect the plasmids from cleavage by extracellular nucleases, they were entrapped into multilayer ALG/PLL microcapsules by layer-by-layer technique. To study the efficacy of humoral immune response, both native and microencapsulated plasmids were injected intramuscular into BALB/c mice. The humoral response in the mice immunized with free plasmids was characterized by virus-neutralizing antibody induction (with titres 1:4 to 1:8), while the injection of microencapsulated plasmids allowed to increase the titre level (from 1:16 to 1:32).
Conclusion
The plasmids microencapsulated in biodegradable MC could be promising for development of DNA vaccines against RVFV.</description><identifier>ISSN: 0141-5492</identifier><identifier>EISSN: 1573-6776</identifier><identifier>DOI: 10.1007/s10529-020-02816-1</identifier><identifier>PMID: 31983039</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Alginates ; Alginic acid ; Antibodies ; Applied Microbiology ; Biochemistry ; Biodegradability ; Biodegradation ; Biomedical and Life Sciences ; Biotechnology ; Coccidioidomycosis ; DNA vaccines ; Fusion protein ; Glycoproteins ; Immune response ; Immune response (humoral) ; Immune system ; Immunization ; Life Sciences ; Lysine ; Microbiology ; Microcapsules ; Multilayers ; Nuclease ; Original Research Paper ; Parainfluenza ; Plasmids ; Poly-L-lysine ; Rift Valley fever ; Rodents ; Vaccines ; Vector-borne diseases ; Viral diseases ; Viruses</subject><ispartof>Biotechnology letters, 2020-04, Vol.42 (4), p.529-536</ispartof><rights>Springer Nature B.V. 2020</rights><rights>Biotechnology Letters is a copyright of Springer, (2020). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c412t-8e02a620b6f6a78acacb746ddb4b98db484f99db35fdad4460fc7cbc676d74663</citedby><cites>FETCH-LOGICAL-c412t-8e02a620b6f6a78acacb746ddb4b98db484f99db35fdad4460fc7cbc676d74663</cites><orcidid>0000-0002-8413-5724</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10529-020-02816-1$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10529-020-02816-1$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31983039$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Selina, Oxana</creatorcontrib><creatorcontrib>Imatdinov, Ilnaz</creatorcontrib><creatorcontrib>Balysheva, Vera</creatorcontrib><creatorcontrib>Akasov, Roman</creatorcontrib><creatorcontrib>Kryukov, Alexander</creatorcontrib><creatorcontrib>Balyshev, Vladimir</creatorcontrib><creatorcontrib>Markvicheva, Elena</creatorcontrib><title>Microencapsulated plasmids expressing Gn and Gc glycoproteins of Rift Valley Fever virus enhance humoral immune response in mice</title><title>Biotechnology letters</title><addtitle>Biotechnol Lett</addtitle><addtitle>Biotechnol Lett</addtitle><description>Objectives
The aim of the current study was to develop biodegradable alginate (ALG)/poly-
l
-lysine (PLL) microcapsules (MC) with entrapped plasmids expressing Gn and Gc glycoproteins of Rift Valley Fever virus (RVFV) and to evaluate the humoral immune response in mice.
Results
Expressing phRVF/Gn and phRVF/Gc plasmids which encode full-sized Gn and Gc glycoproteins and contain signal fusion protein F sequences of human parainfluenza (HPIV-1) were constructed. To protect the plasmids from cleavage by extracellular nucleases, they were entrapped into multilayer ALG/PLL microcapsules by layer-by-layer technique. To study the efficacy of humoral immune response, both native and microencapsulated plasmids were injected intramuscular into BALB/c mice. The humoral response in the mice immunized with free plasmids was characterized by virus-neutralizing antibody induction (with titres 1:4 to 1:8), while the injection of microencapsulated plasmids allowed to increase the titre level (from 1:16 to 1:32).
Conclusion
The plasmids microencapsulated in biodegradable MC could be promising for development of DNA vaccines against RVFV.</description><subject>Alginates</subject><subject>Alginic acid</subject><subject>Antibodies</subject><subject>Applied Microbiology</subject><subject>Biochemistry</subject><subject>Biodegradability</subject><subject>Biodegradation</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Coccidioidomycosis</subject><subject>DNA vaccines</subject><subject>Fusion protein</subject><subject>Glycoproteins</subject><subject>Immune response</subject><subject>Immune response (humoral)</subject><subject>Immune system</subject><subject>Immunization</subject><subject>Life Sciences</subject><subject>Lysine</subject><subject>Microbiology</subject><subject>Microcapsules</subject><subject>Multilayers</subject><subject>Nuclease</subject><subject>Original Research Paper</subject><subject>Parainfluenza</subject><subject>Plasmids</subject><subject>Poly-L-lysine</subject><subject>Rift Valley fever</subject><subject>Rodents</subject><subject>Vaccines</subject><subject>Vector-borne diseases</subject><subject>Viral 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plasmids expressing Gn and Gc glycoproteins of Rift Valley Fever virus enhance humoral immune response in mice</title><author>Selina, Oxana ; Imatdinov, Ilnaz ; Balysheva, Vera ; Akasov, Roman ; Kryukov, Alexander ; Balyshev, Vladimir ; Markvicheva, Elena</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c412t-8e02a620b6f6a78acacb746ddb4b98db484f99db35fdad4460fc7cbc676d74663</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Alginates</topic><topic>Alginic acid</topic><topic>Antibodies</topic><topic>Applied Microbiology</topic><topic>Biochemistry</topic><topic>Biodegradability</topic><topic>Biodegradation</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Coccidioidomycosis</topic><topic>DNA vaccines</topic><topic>Fusion protein</topic><topic>Glycoproteins</topic><topic>Immune response</topic><topic>Immune response (humoral)</topic><topic>Immune system</topic><topic>Immunization</topic><topic>Life Sciences</topic><topic>Lysine</topic><topic>Microbiology</topic><topic>Microcapsules</topic><topic>Multilayers</topic><topic>Nuclease</topic><topic>Original Research Paper</topic><topic>Parainfluenza</topic><topic>Plasmids</topic><topic>Poly-L-lysine</topic><topic>Rift Valley fever</topic><topic>Rodents</topic><topic>Vaccines</topic><topic>Vector-borne diseases</topic><topic>Viral diseases</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Selina, Oxana</creatorcontrib><creatorcontrib>Imatdinov, Ilnaz</creatorcontrib><creatorcontrib>Balysheva, Vera</creatorcontrib><creatorcontrib>Akasov, Roman</creatorcontrib><creatorcontrib>Kryukov, Alexander</creatorcontrib><creatorcontrib>Balyshev, Vladimir</creatorcontrib><creatorcontrib>Markvicheva, 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Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Biotechnology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Selina, Oxana</au><au>Imatdinov, Ilnaz</au><au>Balysheva, Vera</au><au>Akasov, Roman</au><au>Kryukov, Alexander</au><au>Balyshev, Vladimir</au><au>Markvicheva, Elena</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Microencapsulated plasmids expressing Gn and Gc glycoproteins of Rift Valley Fever virus enhance humoral immune response in mice</atitle><jtitle>Biotechnology letters</jtitle><stitle>Biotechnol Lett</stitle><addtitle>Biotechnol Lett</addtitle><date>2020-04-01</date><risdate>2020</risdate><volume>42</volume><issue>4</issue><spage>529</spage><epage>536</epage><pages>529-536</pages><issn>0141-5492</issn><eissn>1573-6776</eissn><abstract>Objectives
The aim of the current study was to develop biodegradable alginate (ALG)/poly-
l
-lysine (PLL) microcapsules (MC) with entrapped plasmids expressing Gn and Gc glycoproteins of Rift Valley Fever virus (RVFV) and to evaluate the humoral immune response in mice.
Results
Expressing phRVF/Gn and phRVF/Gc plasmids which encode full-sized Gn and Gc glycoproteins and contain signal fusion protein F sequences of human parainfluenza (HPIV-1) were constructed. To protect the plasmids from cleavage by extracellular nucleases, they were entrapped into multilayer ALG/PLL microcapsules by layer-by-layer technique. To study the efficacy of humoral immune response, both native and microencapsulated plasmids were injected intramuscular into BALB/c mice. The humoral response in the mice immunized with free plasmids was characterized by virus-neutralizing antibody induction (with titres 1:4 to 1:8), while the injection of microencapsulated plasmids allowed to increase the titre level (from 1:16 to 1:32).
Conclusion
The plasmids microencapsulated in biodegradable MC could be promising for development of DNA vaccines against RVFV.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>31983039</pmid><doi>10.1007/s10529-020-02816-1</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-8413-5724</orcidid></addata></record> |
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| ispartof | Biotechnology letters, 2020-04, Vol.42 (4), p.529-536 |
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| subjects | Alginates Alginic acid Antibodies Applied Microbiology Biochemistry Biodegradability Biodegradation Biomedical and Life Sciences Biotechnology Coccidioidomycosis DNA vaccines Fusion protein Glycoproteins Immune response Immune response (humoral) Immune system Immunization Life Sciences Lysine Microbiology Microcapsules Multilayers Nuclease Original Research Paper Parainfluenza Plasmids Poly-L-lysine Rift Valley fever Rodents Vaccines Vector-borne diseases Viral diseases Viruses |
| title | Microencapsulated plasmids expressing Gn and Gc glycoproteins of Rift Valley Fever virus enhance humoral immune response in mice |
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