Baicalin Reduces Early Brain Injury after Subarachnoid Hemorrhage in Rats
Objective To evaluate the effect of baicalin on subarachnoid hemorrhage (SAH) in rats and explore the potential mechanisms. Methods Sprague-Dawley rats underwent experimental SAH and received treatment with baicalin at 10 or 50 mg/kg after 2 and 12 h of SAH. Neurological scores, brain water content,...
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| Veröffentlicht in: | Chinese journal of integrative medicine 2020-07, Vol.26 (7), p.510-518 |
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| container_title | Chinese journal of integrative medicine |
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| creator | Zhang, Hua-bin Tu, Xian-kun Song, Shi-wei Liang, Ri-sheng Shi, Song-sheng |
| description | Objective
To evaluate the effect of baicalin on subarachnoid hemorrhage (SAH) in rats and explore the potential mechanisms.
Methods
Sprague-Dawley rats underwent experimental SAH and received treatment with baicalin at 10 or 50 mg/kg after 2 and 12 h of SAH. Neurological scores, brain water content, Evans-blue extravasation, and levels of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), myeloperoxidase (MPO), and malondialdehyde (MDA) were measured 24 h after SAH. Expression of nuclear factor erythroid-related factor 2 (Nrf2), NAD(P)H: quinone oxidoreductase 1 (NQO1), matrix metalloproteinase-9 (MMP-9), aquaporin 4 (AQP4), occludin, and zonulaoccludens-1 (ZO-1) were detected in the brain by Western blot. Heme oxygenase-1 (HO-1) was detected by quantitative polymerase chain reaction, and tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) were assessed by enzyme-linked immunosorbent assay.
Results
Baicalin attenuated EBI 24 h after SAH in rats (
P |
| doi_str_mv | 10.1007/s11655-020-3183-7 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2344231205</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2344231205</sourcerecordid><originalsourceid>FETCH-LOGICAL-c410t-51f41c6d133c04e8e3099dd736ce639878a8625dfca968b40fd3db83af9d8f663</originalsourceid><addsrcrecordid>eNp9kEtPwzAQhC0EolD4AVxQjlwC6zi24yOtCq1UCYnH2XL8aFPlUezk0H-PqxSOnHa0OzPSfgjdYXjEAPwpYMwoTSGDlOCCpPwMXWEhSAo5ZOdRM55FjekEXYewA6CcAb1EE4IFB8bZFVrNVKVVXbXJuzWDtiFZKF8fkplXcbdqd4M_JMr11icfQ6m80tu2q0yytE3n_VZtbHLMqj7coAun6mBvT3OKvl4Wn_Nlun57Xc2f16nOMfQpxS7HmhlMiIbcFpaAEMZwwrRlRBS8UAXLqHFaCVaUOThDTFkQ5YQpHGNkih7G3r3vvgcbetlUQdu6Vq3thiAzkucZwRnQaMWjVfsuBG-d3PuqUf4gMcgjQTkSlJGgPBKUPGbuT_VD2Vjzl_hFFg3ZaAjx1G6sl7tu8G18-Z_WH9J7eq0</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2344231205</pqid></control><display><type>article</type><title>Baicalin Reduces Early Brain Injury after Subarachnoid Hemorrhage in Rats</title><source>MEDLINE</source><source>SpringerNature Journals</source><source>Alma/SFX Local Collection</source><creator>Zhang, Hua-bin ; Tu, Xian-kun ; Song, Shi-wei ; Liang, Ri-sheng ; Shi, Song-sheng</creator><creatorcontrib>Zhang, Hua-bin ; Tu, Xian-kun ; Song, Shi-wei ; Liang, Ri-sheng ; Shi, Song-sheng</creatorcontrib><description>Objective
To evaluate the effect of baicalin on subarachnoid hemorrhage (SAH) in rats and explore the potential mechanisms.
Methods
Sprague-Dawley rats underwent experimental SAH and received treatment with baicalin at 10 or 50 mg/kg after 2 and 12 h of SAH. Neurological scores, brain water content, Evans-blue extravasation, and levels of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), myeloperoxidase (MPO), and malondialdehyde (MDA) were measured 24 h after SAH. Expression of nuclear factor erythroid-related factor 2 (Nrf2), NAD(P)H: quinone oxidoreductase 1 (NQO1), matrix metalloproteinase-9 (MMP-9), aquaporin 4 (AQP4), occludin, and zonulaoccludens-1 (ZO-1) were detected in the brain by Western blot. Heme oxygenase-1 (HO-1) was detected by quantitative polymerase chain reaction, and tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) were assessed by enzyme-linked immunosorbent assay.
Results
Baicalin attenuated EBI 24 h after SAH in rats (
P
<0.05). Baicalin elevated neurological scores, GSH-Px, SOD, and increased the expression of Nrf2, NQO1, HO-1, occludin, and ZO-1 in SAH rats (
P
<0.05 or
P
<0.01). Baicalin reduced MPO, MDA, and the expression of MMP-9, AQP4, TNF-α, and IL-1β (
P
<0.05 or
P
<0.01).
Conclusion
Baicalin reduced SAH-induced EBI, partially via activation of the Nrf2/HO-1 pathway and inhibition of MMP-9 and AQP4.</description><identifier>ISSN: 1672-0415</identifier><identifier>EISSN: 1993-0402</identifier><identifier>DOI: 10.1007/s11655-020-3183-7</identifier><identifier>PMID: 31970676</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Animals ; Anti-Inflammatory Agents, Non-Steroidal - pharmacology ; Brain Injuries - drug therapy ; Disease Models, Animal ; Flavonoids - pharmacology ; Male ; Medicine ; Medicine & Public Health ; Original Article ; Rats ; Rats, Sprague-Dawley ; Subarachnoid Hemorrhage - drug therapy</subject><ispartof>Chinese journal of integrative medicine, 2020-07, Vol.26 (7), p.510-518</ispartof><rights>The Chinese Journal of Integrated Traditional and Western Medicine Press and Springer-Verlag GmbH Germany, part of Springer Nature 2020</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c410t-51f41c6d133c04e8e3099dd736ce639878a8625dfca968b40fd3db83af9d8f663</citedby><cites>FETCH-LOGICAL-c410t-51f41c6d133c04e8e3099dd736ce639878a8625dfca968b40fd3db83af9d8f663</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11655-020-3183-7$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11655-020-3183-7$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31970676$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Hua-bin</creatorcontrib><creatorcontrib>Tu, Xian-kun</creatorcontrib><creatorcontrib>Song, Shi-wei</creatorcontrib><creatorcontrib>Liang, Ri-sheng</creatorcontrib><creatorcontrib>Shi, Song-sheng</creatorcontrib><title>Baicalin Reduces Early Brain Injury after Subarachnoid Hemorrhage in Rats</title><title>Chinese journal of integrative medicine</title><addtitle>Chin. J. Integr. Med</addtitle><addtitle>Chin J Integr Med</addtitle><description>Objective
To evaluate the effect of baicalin on subarachnoid hemorrhage (SAH) in rats and explore the potential mechanisms.
Methods
Sprague-Dawley rats underwent experimental SAH and received treatment with baicalin at 10 or 50 mg/kg after 2 and 12 h of SAH. Neurological scores, brain water content, Evans-blue extravasation, and levels of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), myeloperoxidase (MPO), and malondialdehyde (MDA) were measured 24 h after SAH. Expression of nuclear factor erythroid-related factor 2 (Nrf2), NAD(P)H: quinone oxidoreductase 1 (NQO1), matrix metalloproteinase-9 (MMP-9), aquaporin 4 (AQP4), occludin, and zonulaoccludens-1 (ZO-1) were detected in the brain by Western blot. Heme oxygenase-1 (HO-1) was detected by quantitative polymerase chain reaction, and tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) were assessed by enzyme-linked immunosorbent assay.
Results
Baicalin attenuated EBI 24 h after SAH in rats (
P
<0.05). Baicalin elevated neurological scores, GSH-Px, SOD, and increased the expression of Nrf2, NQO1, HO-1, occludin, and ZO-1 in SAH rats (
P
<0.05 or
P
<0.01). Baicalin reduced MPO, MDA, and the expression of MMP-9, AQP4, TNF-α, and IL-1β (
P
<0.05 or
P
<0.01).
Conclusion
Baicalin reduced SAH-induced EBI, partially via activation of the Nrf2/HO-1 pathway and inhibition of MMP-9 and AQP4.</description><subject>Animals</subject><subject>Anti-Inflammatory Agents, Non-Steroidal - pharmacology</subject><subject>Brain Injuries - drug therapy</subject><subject>Disease Models, Animal</subject><subject>Flavonoids - pharmacology</subject><subject>Male</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Original Article</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Subarachnoid Hemorrhage - drug therapy</subject><issn>1672-0415</issn><issn>1993-0402</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtPwzAQhC0EolD4AVxQjlwC6zi24yOtCq1UCYnH2XL8aFPlUezk0H-PqxSOnHa0OzPSfgjdYXjEAPwpYMwoTSGDlOCCpPwMXWEhSAo5ZOdRM55FjekEXYewA6CcAb1EE4IFB8bZFVrNVKVVXbXJuzWDtiFZKF8fkplXcbdqd4M_JMr11icfQ6m80tu2q0yytE3n_VZtbHLMqj7coAun6mBvT3OKvl4Wn_Nlun57Xc2f16nOMfQpxS7HmhlMiIbcFpaAEMZwwrRlRBS8UAXLqHFaCVaUOThDTFkQ5YQpHGNkih7G3r3vvgcbetlUQdu6Vq3thiAzkucZwRnQaMWjVfsuBG-d3PuqUf4gMcgjQTkSlJGgPBKUPGbuT_VD2Vjzl_hFFg3ZaAjx1G6sl7tu8G18-Z_WH9J7eq0</recordid><startdate>20200701</startdate><enddate>20200701</enddate><creator>Zhang, Hua-bin</creator><creator>Tu, Xian-kun</creator><creator>Song, Shi-wei</creator><creator>Liang, Ri-sheng</creator><creator>Shi, Song-sheng</creator><general>Springer Berlin Heidelberg</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20200701</creationdate><title>Baicalin Reduces Early Brain Injury after Subarachnoid Hemorrhage in Rats</title><author>Zhang, Hua-bin ; Tu, Xian-kun ; Song, Shi-wei ; Liang, Ri-sheng ; Shi, Song-sheng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c410t-51f41c6d133c04e8e3099dd736ce639878a8625dfca968b40fd3db83af9d8f663</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Animals</topic><topic>Anti-Inflammatory Agents, Non-Steroidal - pharmacology</topic><topic>Brain Injuries - drug therapy</topic><topic>Disease Models, Animal</topic><topic>Flavonoids - pharmacology</topic><topic>Male</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Original Article</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Subarachnoid Hemorrhage - drug therapy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Hua-bin</creatorcontrib><creatorcontrib>Tu, Xian-kun</creatorcontrib><creatorcontrib>Song, Shi-wei</creatorcontrib><creatorcontrib>Liang, Ri-sheng</creatorcontrib><creatorcontrib>Shi, Song-sheng</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Chinese journal of integrative medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Hua-bin</au><au>Tu, Xian-kun</au><au>Song, Shi-wei</au><au>Liang, Ri-sheng</au><au>Shi, Song-sheng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Baicalin Reduces Early Brain Injury after Subarachnoid Hemorrhage in Rats</atitle><jtitle>Chinese journal of integrative medicine</jtitle><stitle>Chin. J. Integr. Med</stitle><addtitle>Chin J Integr Med</addtitle><date>2020-07-01</date><risdate>2020</risdate><volume>26</volume><issue>7</issue><spage>510</spage><epage>518</epage><pages>510-518</pages><issn>1672-0415</issn><eissn>1993-0402</eissn><abstract>Objective
To evaluate the effect of baicalin on subarachnoid hemorrhage (SAH) in rats and explore the potential mechanisms.
Methods
Sprague-Dawley rats underwent experimental SAH and received treatment with baicalin at 10 or 50 mg/kg after 2 and 12 h of SAH. Neurological scores, brain water content, Evans-blue extravasation, and levels of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), myeloperoxidase (MPO), and malondialdehyde (MDA) were measured 24 h after SAH. Expression of nuclear factor erythroid-related factor 2 (Nrf2), NAD(P)H: quinone oxidoreductase 1 (NQO1), matrix metalloproteinase-9 (MMP-9), aquaporin 4 (AQP4), occludin, and zonulaoccludens-1 (ZO-1) were detected in the brain by Western blot. Heme oxygenase-1 (HO-1) was detected by quantitative polymerase chain reaction, and tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) were assessed by enzyme-linked immunosorbent assay.
Results
Baicalin attenuated EBI 24 h after SAH in rats (
P
<0.05). Baicalin elevated neurological scores, GSH-Px, SOD, and increased the expression of Nrf2, NQO1, HO-1, occludin, and ZO-1 in SAH rats (
P
<0.05 or
P
<0.01). Baicalin reduced MPO, MDA, and the expression of MMP-9, AQP4, TNF-α, and IL-1β (
P
<0.05 or
P
<0.01).
Conclusion
Baicalin reduced SAH-induced EBI, partially via activation of the Nrf2/HO-1 pathway and inhibition of MMP-9 and AQP4.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>31970676</pmid><doi>10.1007/s11655-020-3183-7</doi><tpages>9</tpages></addata></record> |
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| subjects | Animals Anti-Inflammatory Agents, Non-Steroidal - pharmacology Brain Injuries - drug therapy Disease Models, Animal Flavonoids - pharmacology Male Medicine Medicine & Public Health Original Article Rats Rats, Sprague-Dawley Subarachnoid Hemorrhage - drug therapy |
| title | Baicalin Reduces Early Brain Injury after Subarachnoid Hemorrhage in Rats |
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