Optimization and Multiomic Basis of Phenyllactic Acid Overproduction by Lactobacillus plantarum
The goal of this study was to explore the regulatory mechanisms of phenyllactic acid (PLA) overaccumulation in Lactobacillus plantarum. The dynamics of PLA production revealed that 24 h was a suitable fermentation time, at which one of the largest differences in PLA content between strains S1 and YM...
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| Veröffentlicht in: | Journal of agricultural and food chemistry 2020-02, Vol.68 (6), p.1741-1749 |
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| container_title | Journal of agricultural and food chemistry |
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| creator | Wu, Wenyu Deng, Gang Liu, Chenjian Gong, Xiaowei Ma, Guanghui Yuan, Qingqing Yang, En Li, Xiaoran Luo, Yiyong |
| description | The goal of this study was to explore the regulatory mechanisms of phenyllactic acid (PLA) overaccumulation in Lactobacillus plantarum. The dynamics of PLA production revealed that 24 h was a suitable fermentation time, at which one of the largest differences in PLA content between strains S1 and YM-4-3 was 22.42 mg/L. Additionally, an optimization experiment showed that PLA production under the optimal condition (sample YM-4-3y) was up to 400.74 mg/L, 7.61–13.26 times as those of YM-4-3 and S1. Subsequently, an integrated analysis of genomic, transcriptomic and metabolomic data revealed that, YM-4-3 and YM-4-3y, compared with S1, although lacking a complete de novo biosynthetic pathway, increased PLA production by strengthening the core pathway and central carbon metabolism, and weakening the biosynthesis pathway of amino acids and their derivatives. These changes can provide sufficient precursors and compensate for or balance the energy consumed by the reinforced core pathway. |
| doi_str_mv | 10.1021/acs.jafc.9b07136 |
| format | Article |
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The dynamics of PLA production revealed that 24 h was a suitable fermentation time, at which one of the largest differences in PLA content between strains S1 and YM-4-3 was 22.42 mg/L. Additionally, an optimization experiment showed that PLA production under the optimal condition (sample YM-4-3y) was up to 400.74 mg/L, 7.61–13.26 times as those of YM-4-3 and S1. Subsequently, an integrated analysis of genomic, transcriptomic and metabolomic data revealed that, YM-4-3 and YM-4-3y, compared with S1, although lacking a complete de novo biosynthetic pathway, increased PLA production by strengthening the core pathway and central carbon metabolism, and weakening the biosynthesis pathway of amino acids and their derivatives. These changes can provide sufficient precursors and compensate for or balance the energy consumed by the reinforced core pathway.</description><identifier>ISSN: 0021-8561</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/acs.jafc.9b07136</identifier><identifier>PMID: 31964137</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Biosynthetic Pathways ; Fermentation ; Genomics ; Kinetics ; Lactates - chemistry ; Lactates - metabolism ; Lactobacillus plantarum - chemistry ; Lactobacillus plantarum - genetics ; Lactobacillus plantarum - metabolism</subject><ispartof>Journal of agricultural and food chemistry, 2020-02, Vol.68 (6), p.1741-1749</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a336t-b1e90fa1de25d59262e63e2e11e22ef5b8214c481d2c1d6d39bc4a48112569ff3</citedby><cites>FETCH-LOGICAL-a336t-b1e90fa1de25d59262e63e2e11e22ef5b8214c481d2c1d6d39bc4a48112569ff3</cites><orcidid>0000-0002-5910-8054 ; 0000-0002-0273-9218</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.jafc.9b07136$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.jafc.9b07136$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31964137$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wu, Wenyu</creatorcontrib><creatorcontrib>Deng, Gang</creatorcontrib><creatorcontrib>Liu, Chenjian</creatorcontrib><creatorcontrib>Gong, Xiaowei</creatorcontrib><creatorcontrib>Ma, Guanghui</creatorcontrib><creatorcontrib>Yuan, Qingqing</creatorcontrib><creatorcontrib>Yang, En</creatorcontrib><creatorcontrib>Li, Xiaoran</creatorcontrib><creatorcontrib>Luo, Yiyong</creatorcontrib><title>Optimization and Multiomic Basis of Phenyllactic Acid Overproduction by Lactobacillus plantarum</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>The goal of this study was to explore the regulatory mechanisms of phenyllactic acid (PLA) overaccumulation in Lactobacillus plantarum. The dynamics of PLA production revealed that 24 h was a suitable fermentation time, at which one of the largest differences in PLA content between strains S1 and YM-4-3 was 22.42 mg/L. Additionally, an optimization experiment showed that PLA production under the optimal condition (sample YM-4-3y) was up to 400.74 mg/L, 7.61–13.26 times as those of YM-4-3 and S1. Subsequently, an integrated analysis of genomic, transcriptomic and metabolomic data revealed that, YM-4-3 and YM-4-3y, compared with S1, although lacking a complete de novo biosynthetic pathway, increased PLA production by strengthening the core pathway and central carbon metabolism, and weakening the biosynthesis pathway of amino acids and their derivatives. These changes can provide sufficient precursors and compensate for or balance the energy consumed by the reinforced core pathway.</description><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Biosynthetic Pathways</subject><subject>Fermentation</subject><subject>Genomics</subject><subject>Kinetics</subject><subject>Lactates - chemistry</subject><subject>Lactates - metabolism</subject><subject>Lactobacillus plantarum - chemistry</subject><subject>Lactobacillus plantarum - genetics</subject><subject>Lactobacillus plantarum - metabolism</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kDtPwzAUhS0EoqWwM6GMDKT42nGajKXiJRWVAWbL8UO4ygs7Riq_HvcBG5Pt63OOzv0QugQ8BUzgVkg_XQsjp2WFZ0DzIzQGRnDKAIpjNMZRkxYshxE6836NMS7YDJ-iEYUyz4DOxoiv-sE29lsMtmsT0arkJdTx3liZ3AlvfdKZ5PVDt5u6FnKI07m0Kll9ade7TgW581WbZBl_u0pIW9fBJ30t2kG40JyjEyNqry8O5wS9P9y_LZ7S5erxeTFfpoLSfEgr0CU2ApQmTLGS5ETnVBMNoAnRhlUFgUxmBSgiQeWKlpXMRHwDYXlpDJ2g631ubPUZtB94Y73UsXSru-A5oRlluCSR0gThvVS6znunDe-dbYTbcMB8i5VHrHyLlR-wRsvVIT1UjVZ_hl-OUXCzF-ysXXBtXPb_vB8SoITE</recordid><startdate>20200212</startdate><enddate>20200212</enddate><creator>Wu, Wenyu</creator><creator>Deng, Gang</creator><creator>Liu, Chenjian</creator><creator>Gong, Xiaowei</creator><creator>Ma, Guanghui</creator><creator>Yuan, Qingqing</creator><creator>Yang, En</creator><creator>Li, Xiaoran</creator><creator>Luo, Yiyong</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-5910-8054</orcidid><orcidid>https://orcid.org/0000-0002-0273-9218</orcidid></search><sort><creationdate>20200212</creationdate><title>Optimization and Multiomic Basis of Phenyllactic Acid Overproduction by Lactobacillus plantarum</title><author>Wu, Wenyu ; Deng, Gang ; Liu, Chenjian ; Gong, Xiaowei ; Ma, Guanghui ; Yuan, Qingqing ; Yang, En ; Li, Xiaoran ; Luo, Yiyong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a336t-b1e90fa1de25d59262e63e2e11e22ef5b8214c481d2c1d6d39bc4a48112569ff3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Biosynthetic Pathways</topic><topic>Fermentation</topic><topic>Genomics</topic><topic>Kinetics</topic><topic>Lactates - chemistry</topic><topic>Lactates - metabolism</topic><topic>Lactobacillus plantarum - chemistry</topic><topic>Lactobacillus plantarum - genetics</topic><topic>Lactobacillus plantarum - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wu, Wenyu</creatorcontrib><creatorcontrib>Deng, Gang</creatorcontrib><creatorcontrib>Liu, Chenjian</creatorcontrib><creatorcontrib>Gong, Xiaowei</creatorcontrib><creatorcontrib>Ma, Guanghui</creatorcontrib><creatorcontrib>Yuan, Qingqing</creatorcontrib><creatorcontrib>Yang, En</creatorcontrib><creatorcontrib>Li, Xiaoran</creatorcontrib><creatorcontrib>Luo, Yiyong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wu, Wenyu</au><au>Deng, Gang</au><au>Liu, Chenjian</au><au>Gong, Xiaowei</au><au>Ma, Guanghui</au><au>Yuan, Qingqing</au><au>Yang, En</au><au>Li, Xiaoran</au><au>Luo, Yiyong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Optimization and Multiomic Basis of Phenyllactic Acid Overproduction by Lactobacillus plantarum</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>2020-02-12</date><risdate>2020</risdate><volume>68</volume><issue>6</issue><spage>1741</spage><epage>1749</epage><pages>1741-1749</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><abstract>The goal of this study was to explore the regulatory mechanisms of phenyllactic acid (PLA) overaccumulation in Lactobacillus plantarum. The dynamics of PLA production revealed that 24 h was a suitable fermentation time, at which one of the largest differences in PLA content between strains S1 and YM-4-3 was 22.42 mg/L. Additionally, an optimization experiment showed that PLA production under the optimal condition (sample YM-4-3y) was up to 400.74 mg/L, 7.61–13.26 times as those of YM-4-3 and S1. 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| source | MEDLINE; American Chemical Society Journals |
| subjects | Bacterial Proteins - genetics Bacterial Proteins - metabolism Biosynthetic Pathways Fermentation Genomics Kinetics Lactates - chemistry Lactates - metabolism Lactobacillus plantarum - chemistry Lactobacillus plantarum - genetics Lactobacillus plantarum - metabolism |
| title | Optimization and Multiomic Basis of Phenyllactic Acid Overproduction by Lactobacillus plantarum |
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