Establishment of a feeder and serum-free culture system for human embryonic stem cells

Stem cells are an immortal cell population capable of self-renewal; they are essential for human development and ageing and are a major focus of research in regenerative medicine. Despite considerable progress in differentiation of stem cells in vitro, culture conditions require further optimization...

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Veröffentlicht in:	Zygote (Cambridge) 2020-06, Vol.28 (3), p.175-182
Hauptverfasser:	Wang, LiYun, Zhang, RuiNa, Ma, RongHua, Jia, GongXue, Jian, ShengYan, Zeng, XiangHui, Xiong, ZhengFang, Li, BinYe, Li, Chen, Lv, ZhenZhen, Bai, Xue
Format:	Artikel
Sprache:	eng
Schlagworte:	Aging alpha-Fetoproteins - genetics Antibodies Blotting, Western Cell culture Cell Culture Techniques - methods Cell differentiation Cell Differentiation - drug effects Cell Differentiation - genetics Cell Line Cell proliferation Cell Proliferation - drug effects Cell Proliferation - genetics Cell self-renewal Culture Culture Media, Serum-Free - pharmacology Differentiation Embryo cells Embryos Feeder Cells Fetal Proteins - genetics Fibroblasts Gene Expression Genes Growth factors Human Embryonic Stem Cells - cytology Human Embryonic Stem Cells - metabolism Humans Immunofluorescence Kinases Nanog Homeobox Protein - metabolism Nestin Nestin - genetics Oct-4 protein Octamer Transcription Factor-3 - metabolism Optimization Pluripotency Pluripotent Stem Cells - cytology Pluripotent Stem Cells - metabolism Polymerase chain reaction Proteins Regenerative medicine Reverse Transcriptase Polymerase Chain Reaction SOXB1 Transcription Factors - metabolism Stem cell transplantation Stem cells T-Box Domain Proteins - genetics Western blotting α-Fetoprotein
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