Collagen Peptides Isolated from Salmo salar and Tilapia nilotica Skin Accelerate Wound Healing by Altering Cutaneous Microbiome Colonization via Upregulated NOD2 and BD14
Collagen peptides can promote wound healing and are closely related to microbiome colonization. We investigated the relationship among collagen peptides, wound healing, and wound microflora colonization by administering the murine wound model with Salmo salar skin collagen peptides (Ss-SCPs) and Til...
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| Veröffentlicht in: | Journal of agricultural and food chemistry 2020-02, Vol.68 (6), p.1621-1633 |
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| container_title | Journal of agricultural and food chemistry |
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| creator | Mei, Fengfeng Liu, Jingjie Wu, Jintao Duan, Zhouwei Chen, Muxue Meng, Keke Chen, Shenjun Shen, Xuanri Xia, Guanghua Zhao, Meihui |
| description | Collagen peptides can promote wound healing and are closely related to microbiome colonization. We investigated the relationship among collagen peptides, wound healing, and wound microflora colonization by administering the murine wound model with Salmo salar skin collagen peptides (Ss-SCPs) and Tilapia nilotica skin collagen peptides (Tn-SCPs). We analyzed the vascular endothelial growth factor (VEGF), fibroblast growth factors (β-FGF), pattern recognition receptor (NOD2), antimicrobial peptides (β-defence14, BD14), proinflammatory (TNF-α, IL-6, and IL-8) and anti-inflammatory (IL-10) cytokines, macrophages, neutrophil infiltration levels, and microbial communities in the rat wound. The healing rates of the Ss-SCP- and Tn-SCP-treated groups were significantly accelerated, associated with decreased TNF-α, IL-6, and IL-8 and upregulated BD14, NOD2, IL-10, VEGF, and β-FGF. Accelerated healing in the collagen peptide group shows that the wound microflora such as Leuconostoc, Enterococcus, and Bacillus have a positive effect on wound healing (P < 0.01). Other microbiome species such as Stenotrophomonas, Bradyrhizobium, Sphingomonas, and Phyllobacterium had a negative influence and decreased colonization (P < 0.01). Altogether, these studies show that collagen peptide could upregulate wound NOD2 and BD14, which were implicated in microflora colonization regulation in the wound tissue and promoted wound healing by controlling the inflammatory reaction and increasing wound angiogenesis and collagen deposition. |
| doi_str_mv | 10.1021/acs.jafc.9b08002 |
| format | Article |
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We investigated the relationship among collagen peptides, wound healing, and wound microflora colonization by administering the murine wound model with Salmo salar skin collagen peptides (Ss-SCPs) and Tilapia nilotica skin collagen peptides (Tn-SCPs). We analyzed the vascular endothelial growth factor (VEGF), fibroblast growth factors (β-FGF), pattern recognition receptor (NOD2), antimicrobial peptides (β-defence14, BD14), proinflammatory (TNF-α, IL-6, and IL-8) and anti-inflammatory (IL-10) cytokines, macrophages, neutrophil infiltration levels, and microbial communities in the rat wound. The healing rates of the Ss-SCP- and Tn-SCP-treated groups were significantly accelerated, associated with decreased TNF-α, IL-6, and IL-8 and upregulated BD14, NOD2, IL-10, VEGF, and β-FGF. Accelerated healing in the collagen peptide group shows that the wound microflora such as Leuconostoc, Enterococcus, and Bacillus have a positive effect on wound healing (P < 0.01). Other microbiome species such as Stenotrophomonas, Bradyrhizobium, Sphingomonas, and Phyllobacterium had a negative influence and decreased colonization (P < 0.01). Altogether, these studies show that collagen peptide could upregulate wound NOD2 and BD14, which were implicated in microflora colonization regulation in the wound tissue and promoted wound healing by controlling the inflammatory reaction and increasing wound angiogenesis and collagen deposition.</description><identifier>ISSN: 0021-8561</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/acs.jafc.9b08002</identifier><identifier>PMID: 31967468</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Administration, Cutaneous ; Animals ; Bacteria - classification ; Bacteria - drug effects ; Bacteria - genetics ; Bacteria - isolation & purification ; beta-Defensins - genetics ; beta-Defensins - immunology ; Cichlids ; Collagen - chemistry ; Fibroblast Growth Factors - genetics ; Fibroblast Growth Factors - immunology ; Fish Proteins - chemistry ; Humans ; Interleukin-10 - genetics ; Interleukin-10 - immunology ; Male ; Mice ; Microbiota - drug effects ; Nod2 Signaling Adaptor Protein - genetics ; Nod2 Signaling Adaptor Protein - immunology ; Peptides - administration & dosage ; Peptides - chemistry ; Rats ; Rats, Sprague-Dawley ; Salmo salar ; Skin - chemistry ; Tumor Necrosis Factor-alpha - genetics ; Tumor Necrosis Factor-alpha - immunology ; Vascular Endothelial Growth Factor A - genetics ; Vascular Endothelial Growth Factor A - immunology ; Wound Healing - drug effects ; Wounds and Injuries - drug therapy ; Wounds and Injuries - immunology ; Wounds and Injuries - microbiology ; Wounds and Injuries - physiopathology</subject><ispartof>Journal of agricultural and food chemistry, 2020-02, Vol.68 (6), p.1621-1633</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a402t-e75e13520124c0bfbd29b7adfc8d10ac6fce66723a31b5eaf8c6e2936b64b5cd3</citedby><cites>FETCH-LOGICAL-a402t-e75e13520124c0bfbd29b7adfc8d10ac6fce66723a31b5eaf8c6e2936b64b5cd3</cites><orcidid>0000-0001-7269-5751</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.jafc.9b08002$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.jafc.9b08002$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2765,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31967468$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mei, Fengfeng</creatorcontrib><creatorcontrib>Liu, Jingjie</creatorcontrib><creatorcontrib>Wu, Jintao</creatorcontrib><creatorcontrib>Duan, Zhouwei</creatorcontrib><creatorcontrib>Chen, Muxue</creatorcontrib><creatorcontrib>Meng, Keke</creatorcontrib><creatorcontrib>Chen, Shenjun</creatorcontrib><creatorcontrib>Shen, Xuanri</creatorcontrib><creatorcontrib>Xia, Guanghua</creatorcontrib><creatorcontrib>Zhao, Meihui</creatorcontrib><title>Collagen Peptides Isolated from Salmo salar and Tilapia nilotica Skin Accelerate Wound Healing by Altering Cutaneous Microbiome Colonization via Upregulated NOD2 and BD14</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>Collagen peptides can promote wound healing and are closely related to microbiome colonization. We investigated the relationship among collagen peptides, wound healing, and wound microflora colonization by administering the murine wound model with Salmo salar skin collagen peptides (Ss-SCPs) and Tilapia nilotica skin collagen peptides (Tn-SCPs). We analyzed the vascular endothelial growth factor (VEGF), fibroblast growth factors (β-FGF), pattern recognition receptor (NOD2), antimicrobial peptides (β-defence14, BD14), proinflammatory (TNF-α, IL-6, and IL-8) and anti-inflammatory (IL-10) cytokines, macrophages, neutrophil infiltration levels, and microbial communities in the rat wound. The healing rates of the Ss-SCP- and Tn-SCP-treated groups were significantly accelerated, associated with decreased TNF-α, IL-6, and IL-8 and upregulated BD14, NOD2, IL-10, VEGF, and β-FGF. Accelerated healing in the collagen peptide group shows that the wound microflora such as Leuconostoc, Enterococcus, and Bacillus have a positive effect on wound healing (P < 0.01). Other microbiome species such as Stenotrophomonas, Bradyrhizobium, Sphingomonas, and Phyllobacterium had a negative influence and decreased colonization (P < 0.01). Altogether, these studies show that collagen peptide could upregulate wound NOD2 and BD14, which were implicated in microflora colonization regulation in the wound tissue and promoted wound healing by controlling the inflammatory reaction and increasing wound angiogenesis and collagen deposition.</description><subject>Administration, Cutaneous</subject><subject>Animals</subject><subject>Bacteria - classification</subject><subject>Bacteria - drug effects</subject><subject>Bacteria - genetics</subject><subject>Bacteria - isolation & purification</subject><subject>beta-Defensins - genetics</subject><subject>beta-Defensins - immunology</subject><subject>Cichlids</subject><subject>Collagen - chemistry</subject><subject>Fibroblast Growth Factors - genetics</subject><subject>Fibroblast Growth Factors - immunology</subject><subject>Fish Proteins - chemistry</subject><subject>Humans</subject><subject>Interleukin-10 - genetics</subject><subject>Interleukin-10 - immunology</subject><subject>Male</subject><subject>Mice</subject><subject>Microbiota - drug effects</subject><subject>Nod2 Signaling Adaptor Protein - genetics</subject><subject>Nod2 Signaling Adaptor Protein - immunology</subject><subject>Peptides - administration & dosage</subject><subject>Peptides - chemistry</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Salmo salar</subject><subject>Skin - chemistry</subject><subject>Tumor Necrosis Factor-alpha - genetics</subject><subject>Tumor Necrosis Factor-alpha - immunology</subject><subject>Vascular Endothelial Growth Factor A - genetics</subject><subject>Vascular Endothelial Growth Factor A - immunology</subject><subject>Wound Healing - drug effects</subject><subject>Wounds and Injuries - drug therapy</subject><subject>Wounds and Injuries - immunology</subject><subject>Wounds and Injuries - microbiology</subject><subject>Wounds and Injuries - physiopathology</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kc1u1DAUhS1ERYfCnhXykgWZ2k7iJMthCrRSf5DaimV07dyMXBw7tROk8kg8JZ5mYMfKlvWdc-_xIeQdZ2vOBD8FHdcP0Ot1o1jNmHhBVrwULCs5r1-SVXrhWV1Kfkxex_jAGKvLir0ixzlvZFXIekV-b721sENHv-E4mQ4jvYjewoQd7YMf6C3YwdMIFgIF19E7Y2E0QJ2xfjIa6O0P4-hGa7QYkox-93PCzhGscTuqnujGThj29-08gUM_R3pldPDK-AFpmu-d-QWT8Y7-TMb3Y8DdvGxwfXMmnqd-OuPFG3LUg4349nCekPsvn--259nlzdeL7eYyg4KJKcOqRJ6nX-Ci0Ez1qhONqqDrdd1xBlr2GqWsRA45VyVCX2uJosmlkoUqdZefkA-L7xj844xxagcTUzy7LN-KvMiLpqq4SChb0BQnxoB9OwYzQHhqOWv3DbWpoXbfUHtoKEneH9xnNWD3T_C3kgR8XIBnqZ-DS2H_7_cHMgWfQg</recordid><startdate>20200212</startdate><enddate>20200212</enddate><creator>Mei, Fengfeng</creator><creator>Liu, Jingjie</creator><creator>Wu, Jintao</creator><creator>Duan, Zhouwei</creator><creator>Chen, Muxue</creator><creator>Meng, Keke</creator><creator>Chen, Shenjun</creator><creator>Shen, Xuanri</creator><creator>Xia, Guanghua</creator><creator>Zhao, Meihui</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-7269-5751</orcidid></search><sort><creationdate>20200212</creationdate><title>Collagen Peptides Isolated from Salmo salar and Tilapia nilotica Skin Accelerate Wound Healing by Altering Cutaneous Microbiome Colonization via Upregulated NOD2 and BD14</title><author>Mei, Fengfeng ; Liu, Jingjie ; Wu, Jintao ; Duan, Zhouwei ; Chen, Muxue ; Meng, Keke ; Chen, Shenjun ; Shen, Xuanri ; Xia, Guanghua ; Zhao, Meihui</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a402t-e75e13520124c0bfbd29b7adfc8d10ac6fce66723a31b5eaf8c6e2936b64b5cd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Administration, Cutaneous</topic><topic>Animals</topic><topic>Bacteria - classification</topic><topic>Bacteria - drug effects</topic><topic>Bacteria - genetics</topic><topic>Bacteria - isolation & purification</topic><topic>beta-Defensins - genetics</topic><topic>beta-Defensins - immunology</topic><topic>Cichlids</topic><topic>Collagen - chemistry</topic><topic>Fibroblast Growth Factors - genetics</topic><topic>Fibroblast Growth Factors - immunology</topic><topic>Fish Proteins - chemistry</topic><topic>Humans</topic><topic>Interleukin-10 - genetics</topic><topic>Interleukin-10 - immunology</topic><topic>Male</topic><topic>Mice</topic><topic>Microbiota - drug effects</topic><topic>Nod2 Signaling Adaptor Protein - genetics</topic><topic>Nod2 Signaling Adaptor Protein - immunology</topic><topic>Peptides - administration & dosage</topic><topic>Peptides - chemistry</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Salmo salar</topic><topic>Skin - chemistry</topic><topic>Tumor Necrosis Factor-alpha - genetics</topic><topic>Tumor Necrosis Factor-alpha - immunology</topic><topic>Vascular Endothelial Growth Factor A - genetics</topic><topic>Vascular Endothelial Growth Factor A - immunology</topic><topic>Wound Healing - drug effects</topic><topic>Wounds and Injuries - drug therapy</topic><topic>Wounds and Injuries - immunology</topic><topic>Wounds and Injuries - microbiology</topic><topic>Wounds and Injuries - physiopathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mei, Fengfeng</creatorcontrib><creatorcontrib>Liu, Jingjie</creatorcontrib><creatorcontrib>Wu, Jintao</creatorcontrib><creatorcontrib>Duan, Zhouwei</creatorcontrib><creatorcontrib>Chen, Muxue</creatorcontrib><creatorcontrib>Meng, Keke</creatorcontrib><creatorcontrib>Chen, Shenjun</creatorcontrib><creatorcontrib>Shen, Xuanri</creatorcontrib><creatorcontrib>Xia, Guanghua</creatorcontrib><creatorcontrib>Zhao, Meihui</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mei, Fengfeng</au><au>Liu, Jingjie</au><au>Wu, Jintao</au><au>Duan, Zhouwei</au><au>Chen, Muxue</au><au>Meng, Keke</au><au>Chen, Shenjun</au><au>Shen, Xuanri</au><au>Xia, Guanghua</au><au>Zhao, Meihui</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Collagen Peptides Isolated from Salmo salar and Tilapia nilotica Skin Accelerate Wound Healing by Altering Cutaneous Microbiome Colonization via Upregulated NOD2 and BD14</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>2020-02-12</date><risdate>2020</risdate><volume>68</volume><issue>6</issue><spage>1621</spage><epage>1633</epage><pages>1621-1633</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><abstract>Collagen peptides can promote wound healing and are closely related to microbiome colonization. We investigated the relationship among collagen peptides, wound healing, and wound microflora colonization by administering the murine wound model with Salmo salar skin collagen peptides (Ss-SCPs) and Tilapia nilotica skin collagen peptides (Tn-SCPs). We analyzed the vascular endothelial growth factor (VEGF), fibroblast growth factors (β-FGF), pattern recognition receptor (NOD2), antimicrobial peptides (β-defence14, BD14), proinflammatory (TNF-α, IL-6, and IL-8) and anti-inflammatory (IL-10) cytokines, macrophages, neutrophil infiltration levels, and microbial communities in the rat wound. The healing rates of the Ss-SCP- and Tn-SCP-treated groups were significantly accelerated, associated with decreased TNF-α, IL-6, and IL-8 and upregulated BD14, NOD2, IL-10, VEGF, and β-FGF. Accelerated healing in the collagen peptide group shows that the wound microflora such as Leuconostoc, Enterococcus, and Bacillus have a positive effect on wound healing (P < 0.01). Other microbiome species such as Stenotrophomonas, Bradyrhizobium, Sphingomonas, and Phyllobacterium had a negative influence and decreased colonization (P < 0.01). Altogether, these studies show that collagen peptide could upregulate wound NOD2 and BD14, which were implicated in microflora colonization regulation in the wound tissue and promoted wound healing by controlling the inflammatory reaction and increasing wound angiogenesis and collagen deposition.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>31967468</pmid><doi>10.1021/acs.jafc.9b08002</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0001-7269-5751</orcidid></addata></record> |
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| subjects | Administration, Cutaneous Animals Bacteria - classification Bacteria - drug effects Bacteria - genetics Bacteria - isolation & purification beta-Defensins - genetics beta-Defensins - immunology Cichlids Collagen - chemistry Fibroblast Growth Factors - genetics Fibroblast Growth Factors - immunology Fish Proteins - chemistry Humans Interleukin-10 - genetics Interleukin-10 - immunology Male Mice Microbiota - drug effects Nod2 Signaling Adaptor Protein - genetics Nod2 Signaling Adaptor Protein - immunology Peptides - administration & dosage Peptides - chemistry Rats Rats, Sprague-Dawley Salmo salar Skin - chemistry Tumor Necrosis Factor-alpha - genetics Tumor Necrosis Factor-alpha - immunology Vascular Endothelial Growth Factor A - genetics Vascular Endothelial Growth Factor A - immunology Wound Healing - drug effects Wounds and Injuries - drug therapy Wounds and Injuries - immunology Wounds and Injuries - microbiology Wounds and Injuries - physiopathology |
| title | Collagen Peptides Isolated from Salmo salar and Tilapia nilotica Skin Accelerate Wound Healing by Altering Cutaneous Microbiome Colonization via Upregulated NOD2 and BD14 |
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