Sulfate nutrition improves short-term Al3+-stress tolerance in roots of Lolium perenne L
Trivalent aluminum ions (Al3+) in acidic soils are a major constraint for crop productivity inhibiting root elongation and promoting cell death. Al3+-toxicity has adverse biochemical and physiological effects on plant root growth. Sulfur is an essential macronutrient assimilated from the soil in the...
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| Veröffentlicht in: | Plant physiology and biochemistry 2020-03, Vol.148, p.103-113 |
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| creator | Vera-Villalobos, Hernán Lunario-Delgado, Lizzeth Pérez-Retamal, Diana Román, Domingo Leiva, Juan Carlos Zamorano, Pedro Mercado-Seguel, Ana Gálvez, Anita S. Benito, Cesar Wulff-Zottele, Cristián |
| description | Trivalent aluminum ions (Al3+) in acidic soils are a major constraint for crop productivity inhibiting root elongation and promoting cell death. Al3+-toxicity has adverse biochemical and physiological effects on plant root growth. Sulfur is an essential macronutrient assimilated from the soil in the form of sulfate. However, the implication of sulfate nutritional status in the modulation of short-term Al3+-tolerance mechanisms in plant roots has not been previously reported. Here, we evaluated the effects of increased sulfate supply on short-term Al3+-toxicity in roots of Lolium perenne, measuring Al, Ca, Mg and S uptake, lipid peroxidation, total SOD activity, and transcriptional levels of Cu/Zn and Fe-SOD genes. First, the nitrogen sulfur ratio (N/S) in the TF nutrient solutions used in this study were computed to confirm that L. perenne plants were grown in sulfate deficiency (120 μM), optimal supply (240 μM), or overdoses conditions (360 μM), without affecting dry root biomass. Sulfate supplementation (>240 μM, and N/S ratio |
| doi_str_mv | 10.1016/j.plaphy.2020.01.011 |
| format | Article |
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•Sulfate nutrition have a significant effect on the short-term Al3+-stress in roots•Root morphological damage induced by Al3+ is prevented by S supply•Early lipid peroxidation induced by Al3+ stress is reduced by S supply•Adequate S supply modulates the expression of SOD genes involved in Al detoxification•Early intervention with proper sulfate nutrition ameliorates Al toxicity.</description><identifier>ISSN: 0981-9428</identifier><identifier>EISSN: 1873-2690</identifier><identifier>DOI: 10.1016/j.plaphy.2020.01.011</identifier><language>eng</language><publisher>Elsevier Masson SAS</publisher><subject>Al3+-toxicity ; Perennial ryegrass (Lolium perenne) ; Sulfate nutrition ; Superoxide dismutase</subject><ispartof>Plant physiology and biochemistry, 2020-03, Vol.148, p.103-113</ispartof><rights>2020 Elsevier Masson SAS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c269t-b5a6ecfacc8c5c79a2b4184142e00aeb1858ed364cf8df2dc12e8df7cd99c3e53</citedby><cites>FETCH-LOGICAL-c269t-b5a6ecfacc8c5c79a2b4184142e00aeb1858ed364cf8df2dc12e8df7cd99c3e53</cites><orcidid>0000-0002-3397-8318</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0981942820300115$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids></links><search><creatorcontrib>Vera-Villalobos, Hernán</creatorcontrib><creatorcontrib>Lunario-Delgado, Lizzeth</creatorcontrib><creatorcontrib>Pérez-Retamal, Diana</creatorcontrib><creatorcontrib>Román, Domingo</creatorcontrib><creatorcontrib>Leiva, Juan Carlos</creatorcontrib><creatorcontrib>Zamorano, Pedro</creatorcontrib><creatorcontrib>Mercado-Seguel, Ana</creatorcontrib><creatorcontrib>Gálvez, Anita S.</creatorcontrib><creatorcontrib>Benito, Cesar</creatorcontrib><creatorcontrib>Wulff-Zottele, Cristián</creatorcontrib><title>Sulfate nutrition improves short-term Al3+-stress tolerance in roots of Lolium perenne L</title><title>Plant physiology and biochemistry</title><description>Trivalent aluminum ions (Al3+) in acidic soils are a major constraint for crop productivity inhibiting root elongation and promoting cell death. Al3+-toxicity has adverse biochemical and physiological effects on plant root growth. Sulfur is an essential macronutrient assimilated from the soil in the form of sulfate. However, the implication of sulfate nutritional status in the modulation of short-term Al3+-tolerance mechanisms in plant roots has not been previously reported. Here, we evaluated the effects of increased sulfate supply on short-term Al3+-toxicity in roots of Lolium perenne, measuring Al, Ca, Mg and S uptake, lipid peroxidation, total SOD activity, and transcriptional levels of Cu/Zn and Fe-SOD genes. First, the nitrogen sulfur ratio (N/S) in the TF nutrient solutions used in this study were computed to confirm that L. perenne plants were grown in sulfate deficiency (120 μM), optimal supply (240 μM), or overdoses conditions (360 μM), without affecting dry root biomass. Sulfate supplementation (>240 μM, and N/S ratio < 16) played a significant protection to Al3+-stress that prevents morphological changes in root tips, inhibits lipid peroxidation and differentially up-regulates total SOD activity, due changes in SOD gene expression. The results support the importance of sulfate nutritional status, on plant tissue homeostasis, enhancing the physiological tolerance mechanisms modulating lipid peroxidation damage induced by short-term Al3+-toxicity.
•Sulfate nutrition have a significant effect on the short-term Al3+-stress in roots•Root morphological damage induced by Al3+ is prevented by S supply•Early lipid peroxidation induced by Al3+ stress is reduced by S supply•Adequate S supply modulates the expression of SOD genes involved in Al detoxification•Early intervention with proper sulfate nutrition ameliorates Al toxicity.</description><subject>Al3+-toxicity</subject><subject>Perennial ryegrass (Lolium perenne)</subject><subject>Sulfate nutrition</subject><subject>Superoxide dismutase</subject><issn>0981-9428</issn><issn>1873-2690</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp9UEtLAzEYDKJgrf4DDzkKsmse-8hehFJ8wYIHFbyFNPstTclu1iRb6L83pZ6Fge87zAwzg9AtJTkltHrY5ZNV0_aQM8JITmgCPUMLKmqesaoh52hBGkGzpmDiEl2FsCOEsKLmC_T9MdteRcDjHL2Jxo3YDJN3ewg4bJ2PWQQ_4JXl91mIHkLA0VnwatSAzYi9czFg1-PWWTMPeAIP4wi4vUYXvbIBbv7uEn09P32uX7P2_eVtvWoznZLFbFOqCnSvtBa61HWj2KagoqAFA0IUbKgoBXS8KnQvup51mjJIT627ptEcSr5EdyffFPpnhhDlYIIGa9UIbg6S8YJWjDNGE7U4UbV3IXjo5eTNoPxBUiKPQ8qdPA0pj0NKQhOOsseTDFKNvQEvgzaQ-nfGg46yc-Z_g19b-3_p</recordid><startdate>202003</startdate><enddate>202003</enddate><creator>Vera-Villalobos, Hernán</creator><creator>Lunario-Delgado, Lizzeth</creator><creator>Pérez-Retamal, Diana</creator><creator>Román, Domingo</creator><creator>Leiva, Juan Carlos</creator><creator>Zamorano, Pedro</creator><creator>Mercado-Seguel, Ana</creator><creator>Gálvez, Anita S.</creator><creator>Benito, Cesar</creator><creator>Wulff-Zottele, Cristián</creator><general>Elsevier Masson SAS</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-3397-8318</orcidid></search><sort><creationdate>202003</creationdate><title>Sulfate nutrition improves short-term Al3+-stress tolerance in roots of Lolium perenne L</title><author>Vera-Villalobos, Hernán ; Lunario-Delgado, Lizzeth ; Pérez-Retamal, Diana ; Román, Domingo ; Leiva, Juan Carlos ; Zamorano, Pedro ; Mercado-Seguel, Ana ; Gálvez, Anita S. ; Benito, Cesar ; Wulff-Zottele, Cristián</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c269t-b5a6ecfacc8c5c79a2b4184142e00aeb1858ed364cf8df2dc12e8df7cd99c3e53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Al3+-toxicity</topic><topic>Perennial ryegrass (Lolium perenne)</topic><topic>Sulfate nutrition</topic><topic>Superoxide dismutase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vera-Villalobos, Hernán</creatorcontrib><creatorcontrib>Lunario-Delgado, Lizzeth</creatorcontrib><creatorcontrib>Pérez-Retamal, Diana</creatorcontrib><creatorcontrib>Román, Domingo</creatorcontrib><creatorcontrib>Leiva, Juan Carlos</creatorcontrib><creatorcontrib>Zamorano, Pedro</creatorcontrib><creatorcontrib>Mercado-Seguel, Ana</creatorcontrib><creatorcontrib>Gálvez, Anita S.</creatorcontrib><creatorcontrib>Benito, Cesar</creatorcontrib><creatorcontrib>Wulff-Zottele, Cristián</creatorcontrib><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Plant physiology and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vera-Villalobos, Hernán</au><au>Lunario-Delgado, Lizzeth</au><au>Pérez-Retamal, Diana</au><au>Román, Domingo</au><au>Leiva, Juan Carlos</au><au>Zamorano, Pedro</au><au>Mercado-Seguel, Ana</au><au>Gálvez, Anita S.</au><au>Benito, Cesar</au><au>Wulff-Zottele, Cristián</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sulfate nutrition improves short-term Al3+-stress tolerance in roots of Lolium perenne L</atitle><jtitle>Plant physiology and biochemistry</jtitle><date>2020-03</date><risdate>2020</risdate><volume>148</volume><spage>103</spage><epage>113</epage><pages>103-113</pages><issn>0981-9428</issn><eissn>1873-2690</eissn><abstract>Trivalent aluminum ions (Al3+) in acidic soils are a major constraint for crop productivity inhibiting root elongation and promoting cell death. Al3+-toxicity has adverse biochemical and physiological effects on plant root growth. Sulfur is an essential macronutrient assimilated from the soil in the form of sulfate. However, the implication of sulfate nutritional status in the modulation of short-term Al3+-tolerance mechanisms in plant roots has not been previously reported. Here, we evaluated the effects of increased sulfate supply on short-term Al3+-toxicity in roots of Lolium perenne, measuring Al, Ca, Mg and S uptake, lipid peroxidation, total SOD activity, and transcriptional levels of Cu/Zn and Fe-SOD genes. First, the nitrogen sulfur ratio (N/S) in the TF nutrient solutions used in this study were computed to confirm that L. perenne plants were grown in sulfate deficiency (120 μM), optimal supply (240 μM), or overdoses conditions (360 μM), without affecting dry root biomass. Sulfate supplementation (>240 μM, and N/S ratio < 16) played a significant protection to Al3+-stress that prevents morphological changes in root tips, inhibits lipid peroxidation and differentially up-regulates total SOD activity, due changes in SOD gene expression. The results support the importance of sulfate nutritional status, on plant tissue homeostasis, enhancing the physiological tolerance mechanisms modulating lipid peroxidation damage induced by short-term Al3+-toxicity.
•Sulfate nutrition have a significant effect on the short-term Al3+-stress in roots•Root morphological damage induced by Al3+ is prevented by S supply•Early lipid peroxidation induced by Al3+ stress is reduced by S supply•Adequate S supply modulates the expression of SOD genes involved in Al detoxification•Early intervention with proper sulfate nutrition ameliorates Al toxicity.</abstract><pub>Elsevier Masson SAS</pub><doi>10.1016/j.plaphy.2020.01.011</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-3397-8318</orcidid></addata></record> |
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| subjects | Al3+-toxicity Perennial ryegrass (Lolium perenne) Sulfate nutrition Superoxide dismutase |
| title | Sulfate nutrition improves short-term Al3+-stress tolerance in roots of Lolium perenne L |
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