Fluoroquinolone resistance in Haemophilus influenzae from nursing home residents in Taiwan: correlation of MICs and mutations in QRDRs
Aims To study the association between number and positions of mutations with MICs of fluoroquinolone non‐susceptible Haemophilus influenzae. Methods and Results More than 40% of 48 H. influenzae isolated from nursing home residents were not susceptible to fluoroquinolone. Amino acid changes in the q...
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| Veröffentlicht in: | Journal of applied microbiology 2020-06, Vol.128 (6), p.1624-1633 |
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| creator | Chang, C.‐M. Shih, H.‐I. Wu, C.‐J. Lauderdale, T.‐L. Lee, N.‐Y. Lee, C.‐C. Chen, Y.‐C. Huang, C.‐C. Ko, W.‐C. |
| description | Aims
To study the association between number and positions of mutations with MICs of fluoroquinolone non‐susceptible Haemophilus influenzae.
Methods and Results
More than 40% of 48 H. influenzae isolated from nursing home residents were not susceptible to fluoroquinolone. Amino acid changes in the quinolone resistance determining regions, and correlation with MICs and inhibition zone diameters were analysed. All isolates with reduced susceptibility to fluoroquinolones (MIC ≥0·125 µg ml−1) had at least one mutation in gyrA at position 84 and were resistant to nalidixic acid. Compared to isolates with reduced susceptibility, resistant isolates were associated with mutations in gyrA at positions 88 and 134, and in parC at position 88 (P |
| doi_str_mv | 10.1111/jam.14580 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2341610150</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2406220759</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3530-92ba92e40ef0b4dc13c3d8b0af254ffcc998ca1e18a0d54a4b70e5dd58d3143</originalsourceid><addsrcrecordid>eNp10U1vFCEcBnBiNLZWD34BQ-JFD9PyOjvjrVmtrWljrL0TBv5YNgxsYUhTP4Cf29md6sFELrzkxxPIg9BrSo7pPE42ejymQnbkCTqkvJUNa1fs6X4tGklW7AC9KGVDCOVEts_RAae9pKSnh-jXWagpp7vqYwopAs5QfJl0NIB9xOcaxrS99aGWeetChfhTA3Y5jTjWXHz8gW_TuFyzEKcdwzfa3-v4AZuUMwQ9-RRxcvjqYl2wjhaPddof7vG364_X5SV65nQo8OpxPkLfzz7drM-by6-fL9anl43hkpOmZ4PuGQgCjgzCGsoNt91AtGNSOGdM33dGU6CdJlYKLYYVAWmt7Cyngh-hd0vqdvdjKJMafTEQgo6QalGMC9pSQiWZ6dt_6CbVHOe3KSZIyxhZyX5W7xdlciolg1Pb7EedHxQlaleNmqtR-2pm--YxsQ4j2L_yTxczOFnAvQ_w8P8k9eX0aon8DV9vme8</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2406220759</pqid></control><display><type>article</type><title>Fluoroquinolone resistance in Haemophilus influenzae from nursing home residents in Taiwan: correlation of MICs and mutations in QRDRs</title><source>Oxford University Press Journals All Titles (1996-Current)</source><source>Wiley Online Library All Journals</source><creator>Chang, C.‐M. ; Shih, H.‐I. ; Wu, C.‐J. ; Lauderdale, T.‐L. ; Lee, N.‐Y. ; Lee, C.‐C. ; Chen, Y.‐C. ; Huang, C.‐C. ; Ko, W.‐C.</creator><creatorcontrib>Chang, C.‐M. ; Shih, H.‐I. ; Wu, C.‐J. ; Lauderdale, T.‐L. ; Lee, N.‐Y. ; Lee, C.‐C. ; Chen, Y.‐C. ; Huang, C.‐C. ; Ko, W.‐C.</creatorcontrib><description>Aims
To study the association between number and positions of mutations with MICs of fluoroquinolone non‐susceptible Haemophilus influenzae.
Methods and Results
More than 40% of 48 H. influenzae isolated from nursing home residents were not susceptible to fluoroquinolone. Amino acid changes in the quinolone resistance determining regions, and correlation with MICs and inhibition zone diameters were analysed. All isolates with reduced susceptibility to fluoroquinolones (MIC ≥0·125 µg ml−1) had at least one mutation in gyrA at position 84 and were resistant to nalidixic acid. Compared to isolates with reduced susceptibility, resistant isolates were associated with mutations in gyrA at positions 88 and 134, and in parC at position 88 (P < 0·001). Inhibition zone diameter for nalidixic acid disk ≥23 mm may detect susceptible isolates.
Conclusions
Reduced susceptibility to fluoroquinolones was associated with mutations at position 84 in gyrA. A further increase in fluoroquinolone MIC was associated with mutations in gyrA at positions 88 and 134, and parC at position 88.
Significance and Impact of the Study
Due to limited resistant H. influenzae strains, prior studies on association between positions of mutations and fluoroquinolone MICs were inconclusive. The comparison of mutations between isolates with susceptibility, reduced susceptibility and high resistance supported the importance of the present study.</description><identifier>ISSN: 1364-5072</identifier><identifier>EISSN: 1365-2672</identifier><identifier>DOI: 10.1111/jam.14580</identifier><identifier>PMID: 31951091</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Acid resistance ; Amino acid sequence ; Amino acids ; Antibiotics ; fluoroquinolone ; Fluoroquinolones ; Haemophilus influenzae ; High resistance ; Mutation ; Nalidixic acid ; nursing home ; Nursing homes ; quinolone‐resistance determining region ; resistance</subject><ispartof>Journal of applied microbiology, 2020-06, Vol.128 (6), p.1624-1633</ispartof><rights>2020 The Society for Applied Microbiology</rights><rights>2020 The Society for Applied Microbiology.</rights><rights>Copyright © 2020 The Society for Applied Microbiology</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3530-92ba92e40ef0b4dc13c3d8b0af254ffcc998ca1e18a0d54a4b70e5dd58d3143</citedby><cites>FETCH-LOGICAL-c3530-92ba92e40ef0b4dc13c3d8b0af254ffcc998ca1e18a0d54a4b70e5dd58d3143</cites><orcidid>0000-0001-7497-149X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjam.14580$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjam.14580$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31951091$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chang, C.‐M.</creatorcontrib><creatorcontrib>Shih, H.‐I.</creatorcontrib><creatorcontrib>Wu, C.‐J.</creatorcontrib><creatorcontrib>Lauderdale, T.‐L.</creatorcontrib><creatorcontrib>Lee, N.‐Y.</creatorcontrib><creatorcontrib>Lee, C.‐C.</creatorcontrib><creatorcontrib>Chen, Y.‐C.</creatorcontrib><creatorcontrib>Huang, C.‐C.</creatorcontrib><creatorcontrib>Ko, W.‐C.</creatorcontrib><title>Fluoroquinolone resistance in Haemophilus influenzae from nursing home residents in Taiwan: correlation of MICs and mutations in QRDRs</title><title>Journal of applied microbiology</title><addtitle>J Appl Microbiol</addtitle><description>Aims
To study the association between number and positions of mutations with MICs of fluoroquinolone non‐susceptible Haemophilus influenzae.
Methods and Results
More than 40% of 48 H. influenzae isolated from nursing home residents were not susceptible to fluoroquinolone. Amino acid changes in the quinolone resistance determining regions, and correlation with MICs and inhibition zone diameters were analysed. All isolates with reduced susceptibility to fluoroquinolones (MIC ≥0·125 µg ml−1) had at least one mutation in gyrA at position 84 and were resistant to nalidixic acid. Compared to isolates with reduced susceptibility, resistant isolates were associated with mutations in gyrA at positions 88 and 134, and in parC at position 88 (P < 0·001). Inhibition zone diameter for nalidixic acid disk ≥23 mm may detect susceptible isolates.
Conclusions
Reduced susceptibility to fluoroquinolones was associated with mutations at position 84 in gyrA. A further increase in fluoroquinolone MIC was associated with mutations in gyrA at positions 88 and 134, and parC at position 88.
Significance and Impact of the Study
Due to limited resistant H. influenzae strains, prior studies on association between positions of mutations and fluoroquinolone MICs were inconclusive. The comparison of mutations between isolates with susceptibility, reduced susceptibility and high resistance supported the importance of the present study.</description><subject>Acid resistance</subject><subject>Amino acid sequence</subject><subject>Amino acids</subject><subject>Antibiotics</subject><subject>fluoroquinolone</subject><subject>Fluoroquinolones</subject><subject>Haemophilus influenzae</subject><subject>High resistance</subject><subject>Mutation</subject><subject>Nalidixic acid</subject><subject>nursing home</subject><subject>Nursing homes</subject><subject>quinolone‐resistance determining region</subject><subject>resistance</subject><issn>1364-5072</issn><issn>1365-2672</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp10U1vFCEcBnBiNLZWD34BQ-JFD9PyOjvjrVmtrWljrL0TBv5YNgxsYUhTP4Cf29md6sFELrzkxxPIg9BrSo7pPE42ejymQnbkCTqkvJUNa1fs6X4tGklW7AC9KGVDCOVEts_RAae9pKSnh-jXWagpp7vqYwopAs5QfJl0NIB9xOcaxrS99aGWeetChfhTA3Y5jTjWXHz8gW_TuFyzEKcdwzfa3-v4AZuUMwQ9-RRxcvjqYl2wjhaPddof7vG364_X5SV65nQo8OpxPkLfzz7drM-by6-fL9anl43hkpOmZ4PuGQgCjgzCGsoNt91AtGNSOGdM33dGU6CdJlYKLYYVAWmt7Cyngh-hd0vqdvdjKJMafTEQgo6QalGMC9pSQiWZ6dt_6CbVHOe3KSZIyxhZyX5W7xdlciolg1Pb7EedHxQlaleNmqtR-2pm--YxsQ4j2L_yTxczOFnAvQ_w8P8k9eX0aon8DV9vme8</recordid><startdate>202006</startdate><enddate>202006</enddate><creator>Chang, C.‐M.</creator><creator>Shih, H.‐I.</creator><creator>Wu, C.‐J.</creator><creator>Lauderdale, T.‐L.</creator><creator>Lee, N.‐Y.</creator><creator>Lee, C.‐C.</creator><creator>Chen, Y.‐C.</creator><creator>Huang, C.‐C.</creator><creator>Ko, W.‐C.</creator><general>Oxford University Press</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TM</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-7497-149X</orcidid></search><sort><creationdate>202006</creationdate><title>Fluoroquinolone resistance in Haemophilus influenzae from nursing home residents in Taiwan: correlation of MICs and mutations in QRDRs</title><author>Chang, C.‐M. ; Shih, H.‐I. ; Wu, C.‐J. ; Lauderdale, T.‐L. ; Lee, N.‐Y. ; Lee, C.‐C. ; Chen, Y.‐C. ; Huang, C.‐C. ; Ko, W.‐C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3530-92ba92e40ef0b4dc13c3d8b0af254ffcc998ca1e18a0d54a4b70e5dd58d3143</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Acid resistance</topic><topic>Amino acid sequence</topic><topic>Amino acids</topic><topic>Antibiotics</topic><topic>fluoroquinolone</topic><topic>Fluoroquinolones</topic><topic>Haemophilus influenzae</topic><topic>High resistance</topic><topic>Mutation</topic><topic>Nalidixic acid</topic><topic>nursing home</topic><topic>Nursing homes</topic><topic>quinolone‐resistance determining region</topic><topic>resistance</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chang, C.‐M.</creatorcontrib><creatorcontrib>Shih, H.‐I.</creatorcontrib><creatorcontrib>Wu, C.‐J.</creatorcontrib><creatorcontrib>Lauderdale, T.‐L.</creatorcontrib><creatorcontrib>Lee, N.‐Y.</creatorcontrib><creatorcontrib>Lee, C.‐C.</creatorcontrib><creatorcontrib>Chen, Y.‐C.</creatorcontrib><creatorcontrib>Huang, C.‐C.</creatorcontrib><creatorcontrib>Ko, W.‐C.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chang, C.‐M.</au><au>Shih, H.‐I.</au><au>Wu, C.‐J.</au><au>Lauderdale, T.‐L.</au><au>Lee, N.‐Y.</au><au>Lee, C.‐C.</au><au>Chen, Y.‐C.</au><au>Huang, C.‐C.</au><au>Ko, W.‐C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fluoroquinolone resistance in Haemophilus influenzae from nursing home residents in Taiwan: correlation of MICs and mutations in QRDRs</atitle><jtitle>Journal of applied microbiology</jtitle><addtitle>J Appl Microbiol</addtitle><date>2020-06</date><risdate>2020</risdate><volume>128</volume><issue>6</issue><spage>1624</spage><epage>1633</epage><pages>1624-1633</pages><issn>1364-5072</issn><eissn>1365-2672</eissn><abstract>Aims
To study the association between number and positions of mutations with MICs of fluoroquinolone non‐susceptible Haemophilus influenzae.
Methods and Results
More than 40% of 48 H. influenzae isolated from nursing home residents were not susceptible to fluoroquinolone. Amino acid changes in the quinolone resistance determining regions, and correlation with MICs and inhibition zone diameters were analysed. All isolates with reduced susceptibility to fluoroquinolones (MIC ≥0·125 µg ml−1) had at least one mutation in gyrA at position 84 and were resistant to nalidixic acid. Compared to isolates with reduced susceptibility, resistant isolates were associated with mutations in gyrA at positions 88 and 134, and in parC at position 88 (P < 0·001). Inhibition zone diameter for nalidixic acid disk ≥23 mm may detect susceptible isolates.
Conclusions
Reduced susceptibility to fluoroquinolones was associated with mutations at position 84 in gyrA. A further increase in fluoroquinolone MIC was associated with mutations in gyrA at positions 88 and 134, and parC at position 88.
Significance and Impact of the Study
Due to limited resistant H. influenzae strains, prior studies on association between positions of mutations and fluoroquinolone MICs were inconclusive. The comparison of mutations between isolates with susceptibility, reduced susceptibility and high resistance supported the importance of the present study.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>31951091</pmid><doi>10.1111/jam.14580</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0001-7497-149X</orcidid></addata></record> |
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| source | Oxford University Press Journals All Titles (1996-Current); Wiley Online Library All Journals |
| subjects | Acid resistance Amino acid sequence Amino acids Antibiotics fluoroquinolone Fluoroquinolones Haemophilus influenzae High resistance Mutation Nalidixic acid nursing home Nursing homes quinolone‐resistance determining region resistance |
| title | Fluoroquinolone resistance in Haemophilus influenzae from nursing home residents in Taiwan: correlation of MICs and mutations in QRDRs |
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