Enhancing the yield of human erythropoietin in Aspergillus niger by introns and CRISPR-Cas9

Aspergillus niger has been employed to produce heterologous proteins due to its high capacity for expression and secretion; nevertheless, expression levels of human proteins have been modest. We were interested in investigating whether A. niger can express and secret human erythropoietin (HuEPO) at...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Protein expression and purification 2020-04, Vol.168, p.105570-105570, Article 105570
Hauptverfasser:	Rojas-Sánchez, Uriel, López-Calleja, Alberto Cristian, Millán-Chiu, Blanca E., Fernández, Francisco, Loske, Achim M., Gómez-Lim, Miguel A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Aspergillus niger Aspergillus niger - genetics Aspergillus niger - metabolism Cloning, Molecular CRISPR-Cas Systems CRISPR-Cas9 Erythropoietin - biosynthesis Erythropoietin - genetics Fructose-Bisphosphatase - chemistry Fructose-Bisphosphatase - genetics Gene Expression Gene Knockdown Techniques Genes, Fungal Genetic Vectors - chemistry Genetic Vectors - metabolism Glycosylation Humans Intron Introns Plasmids - chemistry Plasmids - metabolism Promoter Regions, Genetic Protein Stability Proteolysis Proteolytic Recombinant Proteins - biosynthesis Recombinant Proteins - genetics rHuEPO RNA, Messenger - genetics RNA, Messenger - metabolism
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!