H2S mediates increased interleukin (IL)-1β and IL-18 production in leukocytes from patients with periodontitis
Background: The mechanisms involved in the interplay between the bacteria and the host cells in periodontitis are not fully understood. Aim: To investigate the effect of the bacterial metabolite H 2 S on the pro-inflammatory cytokines interleukin (IL)-1β and IL-18 from periodontitis patients and hea...
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| Veröffentlicht in: | Journal of oral microbiology 2019-01, Vol.11 (1), p.1617015-1617015 |
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| creator | Basic, Amina Serino, Giovanni Leonhardt, Åsa Dahlén, Gunnar |
| description | Background: The mechanisms involved in the interplay between the bacteria and the host cells in periodontitis are not fully understood.
Aim: To investigate the effect of the bacterial metabolite H
2
S on the pro-inflammatory cytokines interleukin (IL)-1β and IL-18 from periodontitis patients and healthy controls, and to evaluate the composition of the subgingival microbiota with its capacity to produce H
2
S.
Methods: Subgingival bacterial samples from patients with periodontitis (N=32) and healthy controls (N=32) were investigated for H
2
S production and bacterial composition. Peripheral blood mononuclear cells (PBMCs) were cultured in the presence/absence of 1mM H
2
S for 24h and cytokine concentrations were measured.
Results: Subgingival plaque from periodontitis patients had more H
2
S producing bacteria and produced more H
2
S, than healthy controls. PBMCs exposed to H
2
S secreted significantly more IL-1ß and IL-18 (p |
| doi_str_mv | 10.1080/20002297.2019.1617015 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_doaj_</sourceid><recordid>TN_cdi_proquest_miscellaneous_2340034993</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_d29ba4871768454e802865aed1c0dd95</doaj_id><sourcerecordid>2351020857</sourcerecordid><originalsourceid>FETCH-LOGICAL-d479t-1ee6394f69192372f7d234d8b140093d4d42f769e1f191cc0837299f09a22ea13</originalsourceid><addsrcrecordid>eNpdksGO0zAURSMEYkbDfAJSJDbDIsXPdhJ7g0AjmKlUiQWwttz4peOS2sF2pupv8SF80zi0SJSVn-87vovrWxSvgSyACPKOEkIole2CEpALaKAlUD8rLme9mhfP_5kviusYt_lGGBWC05fFBQNouGz4ZeHv6ddyh8bqhLG0rguoI5o8JQwDTj-sK2-Wq7cV_P5VamfK5aoCUY7Bm6lL1rtMljPnu8Ps0Ae_K0edLLoUy71ND-WIwXrjXbLJxlfFi14PEa9P51Xx_fOnb7f31erL3fL246oyvJWpAsSGSd43EiRlLe1bQxk3Yg2cEMkMNzxrjUToQULXEZEhKXsiNaWogV0Vy6Ov8XqrxmB3OhyU11b9EXzYKB2S7QZUhsq15qKFthG85igIFU2t0UBHjJF19qqOXnGP47Q-c9tMo8rSZlIRFRUgCc38-yOf4Rxtl6MIejh7dr5x9kFt_KNqasYpb7LBzckg-J8TxqR2NnY4DNqhn6LKUeTP5FKyjL75D936KbgcbaZqIJSIus3UhyNlXe_DTu99GIxK-jD40AftOhsVA6Lmcqm_5VJzudSpXOwJsdHAMA</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2351020857</pqid></control><display><type>article</type><title>H2S mediates increased interleukin (IL)-1β and IL-18 production in leukocytes from patients with periodontitis</title><source>Taylor & Francis Open Access</source><source>DOAJ Directory of Open Access Journals</source><source>Co-Action Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><creator>Basic, Amina ; Serino, Giovanni ; Leonhardt, Åsa ; Dahlén, Gunnar</creator><creatorcontrib>Basic, Amina ; Serino, Giovanni ; Leonhardt, Åsa ; Dahlén, Gunnar</creatorcontrib><description>Background: The mechanisms involved in the interplay between the bacteria and the host cells in periodontitis are not fully understood.
Aim: To investigate the effect of the bacterial metabolite H
2
S on the pro-inflammatory cytokines interleukin (IL)-1β and IL-18 from periodontitis patients and healthy controls, and to evaluate the composition of the subgingival microbiota with its capacity to produce H
2
S.
Methods: Subgingival bacterial samples from patients with periodontitis (N=32) and healthy controls (N=32) were investigated for H
2
S production and bacterial composition. Peripheral blood mononuclear cells (PBMCs) were cultured in the presence/absence of 1mM H
2
S for 24h and cytokine concentrations were measured.
Results: Subgingival plaque from periodontitis patients had more H
2
S producing bacteria and produced more H
2
S, than healthy controls. PBMCs exposed to H
2
S secreted significantly more IL-1ß and IL-18 (p<0.0001) than untreated control PBMCs from both groups. PBMCs from the periodontitis patients secreted higher levels of the cytokines, both spontaneously (IL-1ß p=0.0001; IL-18 p=0.09) and after exposure to H
2
S (IL-1ß p=0.03; IL-18 p=0.04), which is a new finding not previously reported.
Conclusions: H
2
S, from the subgingival microbiota, can contribute to a host inflammatory response through secretion of the pro-inflammatory cytokines IL-1β and IL-18. Since this response differs between individuals, it may also reflect the susceptibility of the host to develop periodontitis.</description><identifier>ISSN: 2000-2297</identifier><identifier>EISSN: 2000-2297</identifier><identifier>DOI: 10.1080/20002297.2019.1617015</identifier><identifier>PMID: 31164964</identifier><language>eng</language><publisher>Abingdon: Taylor & Francis</publisher><subject>Bacteria ; bismuth plaque test ; Composition ; Cytokines ; Gingiva ; Gum disease ; h2s ; Hydrogen sulfide ; IL-18 ; IL-1β ; Inflammation ; Inflammatory response ; Interleukin 18 ; Leukocytes ; Leukocytes (mononuclear) ; Metabolites ; Microbiota ; Odontologi ; Odontology ; Original ; PBMCs ; Periodontal disease ; Periodontitis ; Peripheral blood mononuclear cells ; Production methods ; qPCR</subject><ispartof>Journal of oral microbiology, 2019-01, Vol.11 (1), p.1617015-1617015</ispartof><rights>2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. 2019</rights><rights>2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. This work is licensed under the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. 2019 The Author(s)</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><orcidid>0000-0003-2217-3642</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6534246/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6534246/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,27479,27901,27902,53766,53768,59116,59117</link.rule.ids><backlink>$$Uhttps://gup.ub.gu.se/publication/281902$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><creatorcontrib>Basic, Amina</creatorcontrib><creatorcontrib>Serino, Giovanni</creatorcontrib><creatorcontrib>Leonhardt, Åsa</creatorcontrib><creatorcontrib>Dahlén, Gunnar</creatorcontrib><title>H2S mediates increased interleukin (IL)-1β and IL-18 production in leukocytes from patients with periodontitis</title><title>Journal of oral microbiology</title><description>Background: The mechanisms involved in the interplay between the bacteria and the host cells in periodontitis are not fully understood.
Aim: To investigate the effect of the bacterial metabolite H
2
S on the pro-inflammatory cytokines interleukin (IL)-1β and IL-18 from periodontitis patients and healthy controls, and to evaluate the composition of the subgingival microbiota with its capacity to produce H
2
S.
Methods: Subgingival bacterial samples from patients with periodontitis (N=32) and healthy controls (N=32) were investigated for H
2
S production and bacterial composition. Peripheral blood mononuclear cells (PBMCs) were cultured in the presence/absence of 1mM H
2
S for 24h and cytokine concentrations were measured.
Results: Subgingival plaque from periodontitis patients had more H
2
S producing bacteria and produced more H
2
S, than healthy controls. PBMCs exposed to H
2
S secreted significantly more IL-1ß and IL-18 (p<0.0001) than untreated control PBMCs from both groups. PBMCs from the periodontitis patients secreted higher levels of the cytokines, both spontaneously (IL-1ß p=0.0001; IL-18 p=0.09) and after exposure to H
2
S (IL-1ß p=0.03; IL-18 p=0.04), which is a new finding not previously reported.
Conclusions: H
2
S, from the subgingival microbiota, can contribute to a host inflammatory response through secretion of the pro-inflammatory cytokines IL-1β and IL-18. Since this response differs between individuals, it may also reflect the susceptibility of the host to develop periodontitis.</description><subject>Bacteria</subject><subject>bismuth plaque test</subject><subject>Composition</subject><subject>Cytokines</subject><subject>Gingiva</subject><subject>Gum disease</subject><subject>h2s</subject><subject>Hydrogen sulfide</subject><subject>IL-18</subject><subject>IL-1β</subject><subject>Inflammation</subject><subject>Inflammatory response</subject><subject>Interleukin 18</subject><subject>Leukocytes</subject><subject>Leukocytes (mononuclear)</subject><subject>Metabolites</subject><subject>Microbiota</subject><subject>Odontologi</subject><subject>Odontology</subject><subject>Original</subject><subject>PBMCs</subject><subject>Periodontal disease</subject><subject>Periodontitis</subject><subject>Peripheral blood mononuclear cells</subject><subject>Production methods</subject><subject>qPCR</subject><issn>2000-2297</issn><issn>2000-2297</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>0YH</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNpdksGO0zAURSMEYkbDfAJSJDbDIsXPdhJ7g0AjmKlUiQWwttz4peOS2sF2pupv8SF80zi0SJSVn-87vovrWxSvgSyACPKOEkIole2CEpALaKAlUD8rLme9mhfP_5kviusYt_lGGBWC05fFBQNouGz4ZeHv6ddyh8bqhLG0rguoI5o8JQwDTj-sK2-Wq7cV_P5VamfK5aoCUY7Bm6lL1rtMljPnu8Ps0Ae_K0edLLoUy71ND-WIwXrjXbLJxlfFi14PEa9P51Xx_fOnb7f31erL3fL246oyvJWpAsSGSd43EiRlLe1bQxk3Yg2cEMkMNzxrjUToQULXEZEhKXsiNaWogV0Vy6Ov8XqrxmB3OhyU11b9EXzYKB2S7QZUhsq15qKFthG85igIFU2t0UBHjJF19qqOXnGP47Q-c9tMo8rSZlIRFRUgCc38-yOf4Rxtl6MIejh7dr5x9kFt_KNqasYpb7LBzckg-J8TxqR2NnY4DNqhn6LKUeTP5FKyjL75D936KbgcbaZqIJSIus3UhyNlXe_DTu99GIxK-jD40AftOhsVA6Lmcqm_5VJzudSpXOwJsdHAMA</recordid><startdate>20190101</startdate><enddate>20190101</enddate><creator>Basic, Amina</creator><creator>Serino, Giovanni</creator><creator>Leonhardt, Åsa</creator><creator>Dahlén, Gunnar</creator><general>Taylor & Francis</general><general>Taylor & Francis Ltd</general><general>Taylor & Francis Group</general><scope>0YH</scope><scope>3V.</scope><scope>7QP</scope><scope>7T7</scope><scope>7X7</scope><scope>7XB</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M7P</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><scope>ADTPV</scope><scope>AOWAS</scope><scope>F1U</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0003-2217-3642</orcidid></search><sort><creationdate>20190101</creationdate><title>H2S mediates increased interleukin (IL)-1β and IL-18 production in leukocytes from patients with periodontitis</title><author>Basic, Amina ; Serino, Giovanni ; Leonhardt, Åsa ; Dahlén, Gunnar</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-d479t-1ee6394f69192372f7d234d8b140093d4d42f769e1f191cc0837299f09a22ea13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Bacteria</topic><topic>bismuth plaque test</topic><topic>Composition</topic><topic>Cytokines</topic><topic>Gingiva</topic><topic>Gum disease</topic><topic>h2s</topic><topic>Hydrogen sulfide</topic><topic>IL-18</topic><topic>IL-1β</topic><topic>Inflammation</topic><topic>Inflammatory response</topic><topic>Interleukin 18</topic><topic>Leukocytes</topic><topic>Leukocytes (mononuclear)</topic><topic>Metabolites</topic><topic>Microbiota</topic><topic>Odontologi</topic><topic>Odontology</topic><topic>Original</topic><topic>PBMCs</topic><topic>Periodontal disease</topic><topic>Periodontitis</topic><topic>Peripheral blood mononuclear cells</topic><topic>Production methods</topic><topic>qPCR</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Basic, Amina</creatorcontrib><creatorcontrib>Serino, Giovanni</creatorcontrib><creatorcontrib>Leonhardt, Åsa</creatorcontrib><creatorcontrib>Dahlén, Gunnar</creatorcontrib><collection>Taylor & Francis Open Access</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>SwePub</collection><collection>SwePub Articles</collection><collection>SWEPUB Göteborgs universitet</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Journal of oral microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Basic, Amina</au><au>Serino, Giovanni</au><au>Leonhardt, Åsa</au><au>Dahlén, Gunnar</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>H2S mediates increased interleukin (IL)-1β and IL-18 production in leukocytes from patients with periodontitis</atitle><jtitle>Journal of oral microbiology</jtitle><date>2019-01-01</date><risdate>2019</risdate><volume>11</volume><issue>1</issue><spage>1617015</spage><epage>1617015</epage><pages>1617015-1617015</pages><issn>2000-2297</issn><eissn>2000-2297</eissn><abstract>Background: The mechanisms involved in the interplay between the bacteria and the host cells in periodontitis are not fully understood.
Aim: To investigate the effect of the bacterial metabolite H
2
S on the pro-inflammatory cytokines interleukin (IL)-1β and IL-18 from periodontitis patients and healthy controls, and to evaluate the composition of the subgingival microbiota with its capacity to produce H
2
S.
Methods: Subgingival bacterial samples from patients with periodontitis (N=32) and healthy controls (N=32) were investigated for H
2
S production and bacterial composition. Peripheral blood mononuclear cells (PBMCs) were cultured in the presence/absence of 1mM H
2
S for 24h and cytokine concentrations were measured.
Results: Subgingival plaque from periodontitis patients had more H
2
S producing bacteria and produced more H
2
S, than healthy controls. PBMCs exposed to H
2
S secreted significantly more IL-1ß and IL-18 (p<0.0001) than untreated control PBMCs from both groups. PBMCs from the periodontitis patients secreted higher levels of the cytokines, both spontaneously (IL-1ß p=0.0001; IL-18 p=0.09) and after exposure to H
2
S (IL-1ß p=0.03; IL-18 p=0.04), which is a new finding not previously reported.
Conclusions: H
2
S, from the subgingival microbiota, can contribute to a host inflammatory response through secretion of the pro-inflammatory cytokines IL-1β and IL-18. Since this response differs between individuals, it may also reflect the susceptibility of the host to develop periodontitis.</abstract><cop>Abingdon</cop><pub>Taylor & Francis</pub><pmid>31164964</pmid><doi>10.1080/20002297.2019.1617015</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0003-2217-3642</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of oral microbiology, 2019-01, Vol.11 (1), p.1617015-1617015 |
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| source | Taylor & Francis Open Access; DOAJ Directory of Open Access Journals; Co-Action Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Bacteria bismuth plaque test Composition Cytokines Gingiva Gum disease h2s Hydrogen sulfide IL-18 IL-1β Inflammation Inflammatory response Interleukin 18 Leukocytes Leukocytes (mononuclear) Metabolites Microbiota Odontologi Odontology Original PBMCs Periodontal disease Periodontitis Peripheral blood mononuclear cells Production methods qPCR |
| title | H2S mediates increased interleukin (IL)-1β and IL-18 production in leukocytes from patients with periodontitis |
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