Metabolomics of endemic six Astragalus species by combined NMR and GC‐MS analysis
Introduction Astragalus anthylloides, A. dipsaceus, A. karamasicus, A. lycius, A. sigmoideus and A. xylobasis var. angustus are an endemic and generally grow in the Irano‐Turanian phytogeographic region of Turkey. Astragalus species contain saponins, polysaccharides, and phenolics, while the toxic c...
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| creator | Sahin Yaglioglu, Ayse Temirturk, Murat Ugur, Emic Dolarslan, Melda Demirtas, Ibrahim |
| description | Introduction
Astragalus anthylloides, A. dipsaceus, A. karamasicus, A. lycius, A. sigmoideus and A. xylobasis var. angustus are an endemic and generally grow in the Irano‐Turanian phytogeographic region of Turkey. Astragalus species contain saponins, polysaccharides, and phenolics, while the toxic compounds include imidazoline alkaloids, nitro toxins, and selenium derivatives.
Objectives
To apply a combined metabolomic fingerprinting approach by nuclear magnetic resonance (NMR) and gas chromatography‐mass spectrometry (GC‐MS) of endemic six Astragalus species extract.
Methodology
The whole plant collected in Turkey of six endemic Astragalus subsp. were dried and then extracted with hexane, chloroform, ethylacetate, n‐butanol and methanol solvents, respectively. The hexane extracts were analyzed by GC‐MS. Carbon‐13 (13C)‐NMR analyzes of all extracts were performed. In both analyses, a biomarker was obtained.
Results
The hexane extracts were determined as palmitic acid, arachidic acid, behenic acid, and linolenic acid as the main components. As a result of 13C‐NMR analyzes, in hexane, chloroform, and ethylacetate the extracts detected were palmitic acid, arachidic acid, behenic acid, and linolenic acid. d‐Pinitol was obtained using 13C‐NMR analyzes with n‐butanol and methanol extracts.
Conclusion
This study demonstrated that d‐pinitol is a biomarker for the endemic six Astragalus subsp.
Introduction: The six Astragalus species are an endemic to Turkey. Objectives: to apply a combined metabolomic fingerprinting approach by NMR and GC‐MS of six Astragalus species extract. Methodology: The six Astragalus were exracted with solvent at different polarity. Then, the extracts were analyzed by GC‐MS and 13CNMR analyzes to determined the biomarkers. Results: D‐Pinitol was obtained at 13C NMR analyzes of the n‐BuOH and MeOH extracts. Conclusion:D‐pinitol is a biomarker for the species. |
| doi_str_mv | 10.1002/pca.2896 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2339790812</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2387122944</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3496-d13099ba2ba7a477159783280aec8fb4a27b29dcb0bac341df20230196b5a7f23</originalsourceid><addsrcrecordid>eNp1kN9KwzAUh4Mobk7BJ5CAN9505l-b5nIMncKm4vS6JGkqHW1TmxXtnY_gM_okpm4qCF6dH5zvfBx-ABxjNMYIkfNayzGJRbQDhhgJEeAwCnfBEIkwDhBlbAAOnFsh5Hci2gcDigWjLCJDsFyYtVS2sGWuHbQZNFVqfIYuf4UTt27kkyxaB11tdG4cVB3UtlR5ZVJ4s7iHskrhbPrx9r5Y-iyLzuXuEOxlsnDmaDtH4PHy4mF6FcxvZ9fTyTzQlIkoSDH1_yhJlOSScY5DwWNKYiSNjjPFJOGKiFQrpKS_wGlGEKEIi0iFkmeEjsDZxls39rk1bp2UudOmKGRlbOsSQqngAsW4R0__oCvbNv7fnoo5JkQw9ivUjXWuMVlSN3kpmy7BKOmLTnzRSV-0R0-2wlaVJv0Bv5v1QLABXvLCdP-Kkrvp5Ev4CVF3hbY</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2387122944</pqid></control><display><type>article</type><title>Metabolomics of endemic six Astragalus species by combined NMR and GC‐MS analysis</title><source>Wiley Online Library - AutoHoldings Journals</source><source>MEDLINE</source><creator>Sahin Yaglioglu, Ayse ; Temirturk, Murat ; Ugur, Emic ; Dolarslan, Melda ; Demirtas, Ibrahim</creator><creatorcontrib>Sahin Yaglioglu, Ayse ; Temirturk, Murat ; Ugur, Emic ; Dolarslan, Melda ; Demirtas, Ibrahim</creatorcontrib><description>Introduction
Astragalus anthylloides, A. dipsaceus, A. karamasicus, A. lycius, A. sigmoideus and A. xylobasis var. angustus are an endemic and generally grow in the Irano‐Turanian phytogeographic region of Turkey. Astragalus species contain saponins, polysaccharides, and phenolics, while the toxic compounds include imidazoline alkaloids, nitro toxins, and selenium derivatives.
Objectives
To apply a combined metabolomic fingerprinting approach by nuclear magnetic resonance (NMR) and gas chromatography‐mass spectrometry (GC‐MS) of endemic six Astragalus species extract.
Methodology
The whole plant collected in Turkey of six endemic Astragalus subsp. were dried and then extracted with hexane, chloroform, ethylacetate, n‐butanol and methanol solvents, respectively. The hexane extracts were analyzed by GC‐MS. Carbon‐13 (13C)‐NMR analyzes of all extracts were performed. In both analyses, a biomarker was obtained.
Results
The hexane extracts were determined as palmitic acid, arachidic acid, behenic acid, and linolenic acid as the main components. As a result of 13C‐NMR analyzes, in hexane, chloroform, and ethylacetate the extracts detected were palmitic acid, arachidic acid, behenic acid, and linolenic acid. d‐Pinitol was obtained using 13C‐NMR analyzes with n‐butanol and methanol extracts.
Conclusion
This study demonstrated that d‐pinitol is a biomarker for the endemic six Astragalus subsp.
Introduction: The six Astragalus species are an endemic to Turkey. Objectives: to apply a combined metabolomic fingerprinting approach by NMR and GC‐MS of six Astragalus species extract. Methodology: The six Astragalus were exracted with solvent at different polarity. Then, the extracts were analyzed by GC‐MS and 13CNMR analyzes to determined the biomarkers. Results: D‐Pinitol was obtained at 13C NMR analyzes of the n‐BuOH and MeOH extracts. Conclusion:D‐pinitol is a biomarker for the species.</description><identifier>ISSN: 0958-0344</identifier><identifier>EISSN: 1099-1565</identifier><identifier>DOI: 10.1002/pca.2896</identifier><identifier>PMID: 31943462</identifier><language>eng</language><publisher>England: Wiley Subscription Services, Inc</publisher><subject>Acids ; Alkaloids ; Astragalus anthylloides ; Astragalus dipsaceus ; Astragalus karamasicus ; Astragalus lycius ; Astragalus sigmoideus ; Astragalus subsp ; Astragalus xylobasis angustus ; biomarker ; Biomarkers ; Butanol ; Carbon 13 ; Chloroform ; d‐pinitol ; endemic ; Endemic species ; Fingerprinting ; Gas chromatography ; Gas Chromatography-Mass Spectrometry ; Imidazoline ; Linolenic acid ; Magnetic Resonance Spectroscopy ; Mass spectrometry ; Mass spectroscopy ; Metabolomics ; Methanol ; NMR ; Nuclear magnetic resonance ; Palmitic acid ; Phenols ; Plant Extracts ; Polysaccharides ; Saccharides ; Saponins ; Selenium ; Toxins ; Turkey</subject><ispartof>Phytochemical analysis, 2020-05, Vol.31 (3), p.306-313</ispartof><rights>2020 John Wiley & Sons, Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3496-d13099ba2ba7a477159783280aec8fb4a27b29dcb0bac341df20230196b5a7f23</citedby><cites>FETCH-LOGICAL-c3496-d13099ba2ba7a477159783280aec8fb4a27b29dcb0bac341df20230196b5a7f23</cites><orcidid>0000-0002-7668-6286</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fpca.2896$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fpca.2896$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27915,27916,45565,45566</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31943462$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sahin Yaglioglu, Ayse</creatorcontrib><creatorcontrib>Temirturk, Murat</creatorcontrib><creatorcontrib>Ugur, Emic</creatorcontrib><creatorcontrib>Dolarslan, Melda</creatorcontrib><creatorcontrib>Demirtas, Ibrahim</creatorcontrib><title>Metabolomics of endemic six Astragalus species by combined NMR and GC‐MS analysis</title><title>Phytochemical analysis</title><addtitle>Phytochem Anal</addtitle><description>Introduction
Astragalus anthylloides, A. dipsaceus, A. karamasicus, A. lycius, A. sigmoideus and A. xylobasis var. angustus are an endemic and generally grow in the Irano‐Turanian phytogeographic region of Turkey. Astragalus species contain saponins, polysaccharides, and phenolics, while the toxic compounds include imidazoline alkaloids, nitro toxins, and selenium derivatives.
Objectives
To apply a combined metabolomic fingerprinting approach by nuclear magnetic resonance (NMR) and gas chromatography‐mass spectrometry (GC‐MS) of endemic six Astragalus species extract.
Methodology
The whole plant collected in Turkey of six endemic Astragalus subsp. were dried and then extracted with hexane, chloroform, ethylacetate, n‐butanol and methanol solvents, respectively. The hexane extracts were analyzed by GC‐MS. Carbon‐13 (13C)‐NMR analyzes of all extracts were performed. In both analyses, a biomarker was obtained.
Results
The hexane extracts were determined as palmitic acid, arachidic acid, behenic acid, and linolenic acid as the main components. As a result of 13C‐NMR analyzes, in hexane, chloroform, and ethylacetate the extracts detected were palmitic acid, arachidic acid, behenic acid, and linolenic acid. d‐Pinitol was obtained using 13C‐NMR analyzes with n‐butanol and methanol extracts.
Conclusion
This study demonstrated that d‐pinitol is a biomarker for the endemic six Astragalus subsp.
Introduction: The six Astragalus species are an endemic to Turkey. Objectives: to apply a combined metabolomic fingerprinting approach by NMR and GC‐MS of six Astragalus species extract. Methodology: The six Astragalus were exracted with solvent at different polarity. Then, the extracts were analyzed by GC‐MS and 13CNMR analyzes to determined the biomarkers. Results: D‐Pinitol was obtained at 13C NMR analyzes of the n‐BuOH and MeOH extracts. Conclusion:D‐pinitol is a biomarker for the species.</description><subject>Acids</subject><subject>Alkaloids</subject><subject>Astragalus anthylloides</subject><subject>Astragalus dipsaceus</subject><subject>Astragalus karamasicus</subject><subject>Astragalus lycius</subject><subject>Astragalus sigmoideus</subject><subject>Astragalus subsp</subject><subject>Astragalus xylobasis angustus</subject><subject>biomarker</subject><subject>Biomarkers</subject><subject>Butanol</subject><subject>Carbon 13</subject><subject>Chloroform</subject><subject>d‐pinitol</subject><subject>endemic</subject><subject>Endemic species</subject><subject>Fingerprinting</subject><subject>Gas chromatography</subject><subject>Gas Chromatography-Mass Spectrometry</subject><subject>Imidazoline</subject><subject>Linolenic acid</subject><subject>Magnetic Resonance Spectroscopy</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Metabolomics</subject><subject>Methanol</subject><subject>NMR</subject><subject>Nuclear magnetic resonance</subject><subject>Palmitic acid</subject><subject>Phenols</subject><subject>Plant Extracts</subject><subject>Polysaccharides</subject><subject>Saccharides</subject><subject>Saponins</subject><subject>Selenium</subject><subject>Toxins</subject><subject>Turkey</subject><issn>0958-0344</issn><issn>1099-1565</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kN9KwzAUh4Mobk7BJ5CAN9505l-b5nIMncKm4vS6JGkqHW1TmxXtnY_gM_okpm4qCF6dH5zvfBx-ABxjNMYIkfNayzGJRbQDhhgJEeAwCnfBEIkwDhBlbAAOnFsh5Hci2gcDigWjLCJDsFyYtVS2sGWuHbQZNFVqfIYuf4UTt27kkyxaB11tdG4cVB3UtlR5ZVJ4s7iHskrhbPrx9r5Y-iyLzuXuEOxlsnDmaDtH4PHy4mF6FcxvZ9fTyTzQlIkoSDH1_yhJlOSScY5DwWNKYiSNjjPFJOGKiFQrpKS_wGlGEKEIi0iFkmeEjsDZxls39rk1bp2UudOmKGRlbOsSQqngAsW4R0__oCvbNv7fnoo5JkQw9ivUjXWuMVlSN3kpmy7BKOmLTnzRSV-0R0-2wlaVJv0Bv5v1QLABXvLCdP-Kkrvp5Ev4CVF3hbY</recordid><startdate>202005</startdate><enddate>202005</enddate><creator>Sahin Yaglioglu, Ayse</creator><creator>Temirturk, Murat</creator><creator>Ugur, Emic</creator><creator>Dolarslan, Melda</creator><creator>Demirtas, Ibrahim</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QR</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-7668-6286</orcidid></search><sort><creationdate>202005</creationdate><title>Metabolomics of endemic six Astragalus species by combined NMR and GC‐MS analysis</title><author>Sahin Yaglioglu, Ayse ; Temirturk, Murat ; Ugur, Emic ; Dolarslan, Melda ; Demirtas, Ibrahim</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3496-d13099ba2ba7a477159783280aec8fb4a27b29dcb0bac341df20230196b5a7f23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Acids</topic><topic>Alkaloids</topic><topic>Astragalus anthylloides</topic><topic>Astragalus dipsaceus</topic><topic>Astragalus karamasicus</topic><topic>Astragalus lycius</topic><topic>Astragalus sigmoideus</topic><topic>Astragalus subsp</topic><topic>Astragalus xylobasis angustus</topic><topic>biomarker</topic><topic>Biomarkers</topic><topic>Butanol</topic><topic>Carbon 13</topic><topic>Chloroform</topic><topic>d‐pinitol</topic><topic>endemic</topic><topic>Endemic species</topic><topic>Fingerprinting</topic><topic>Gas chromatography</topic><topic>Gas Chromatography-Mass Spectrometry</topic><topic>Imidazoline</topic><topic>Linolenic acid</topic><topic>Magnetic Resonance Spectroscopy</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>Metabolomics</topic><topic>Methanol</topic><topic>NMR</topic><topic>Nuclear magnetic resonance</topic><topic>Palmitic acid</topic><topic>Phenols</topic><topic>Plant Extracts</topic><topic>Polysaccharides</topic><topic>Saccharides</topic><topic>Saponins</topic><topic>Selenium</topic><topic>Toxins</topic><topic>Turkey</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sahin Yaglioglu, Ayse</creatorcontrib><creatorcontrib>Temirturk, Murat</creatorcontrib><creatorcontrib>Ugur, Emic</creatorcontrib><creatorcontrib>Dolarslan, Melda</creatorcontrib><creatorcontrib>Demirtas, Ibrahim</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Chemoreception Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Phytochemical analysis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sahin Yaglioglu, Ayse</au><au>Temirturk, Murat</au><au>Ugur, Emic</au><au>Dolarslan, Melda</au><au>Demirtas, Ibrahim</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Metabolomics of endemic six Astragalus species by combined NMR and GC‐MS analysis</atitle><jtitle>Phytochemical analysis</jtitle><addtitle>Phytochem Anal</addtitle><date>2020-05</date><risdate>2020</risdate><volume>31</volume><issue>3</issue><spage>306</spage><epage>313</epage><pages>306-313</pages><issn>0958-0344</issn><eissn>1099-1565</eissn><abstract>Introduction
Astragalus anthylloides, A. dipsaceus, A. karamasicus, A. lycius, A. sigmoideus and A. xylobasis var. angustus are an endemic and generally grow in the Irano‐Turanian phytogeographic region of Turkey. Astragalus species contain saponins, polysaccharides, and phenolics, while the toxic compounds include imidazoline alkaloids, nitro toxins, and selenium derivatives.
Objectives
To apply a combined metabolomic fingerprinting approach by nuclear magnetic resonance (NMR) and gas chromatography‐mass spectrometry (GC‐MS) of endemic six Astragalus species extract.
Methodology
The whole plant collected in Turkey of six endemic Astragalus subsp. were dried and then extracted with hexane, chloroform, ethylacetate, n‐butanol and methanol solvents, respectively. The hexane extracts were analyzed by GC‐MS. Carbon‐13 (13C)‐NMR analyzes of all extracts were performed. In both analyses, a biomarker was obtained.
Results
The hexane extracts were determined as palmitic acid, arachidic acid, behenic acid, and linolenic acid as the main components. As a result of 13C‐NMR analyzes, in hexane, chloroform, and ethylacetate the extracts detected were palmitic acid, arachidic acid, behenic acid, and linolenic acid. d‐Pinitol was obtained using 13C‐NMR analyzes with n‐butanol and methanol extracts.
Conclusion
This study demonstrated that d‐pinitol is a biomarker for the endemic six Astragalus subsp.
Introduction: The six Astragalus species are an endemic to Turkey. Objectives: to apply a combined metabolomic fingerprinting approach by NMR and GC‐MS of six Astragalus species extract. Methodology: The six Astragalus were exracted with solvent at different polarity. Then, the extracts were analyzed by GC‐MS and 13CNMR analyzes to determined the biomarkers. Results: D‐Pinitol was obtained at 13C NMR analyzes of the n‐BuOH and MeOH extracts. Conclusion:D‐pinitol is a biomarker for the species.</abstract><cop>England</cop><pub>Wiley Subscription Services, Inc</pub><pmid>31943462</pmid><doi>10.1002/pca.2896</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-7668-6286</orcidid></addata></record> |
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| subjects | Acids Alkaloids Astragalus anthylloides Astragalus dipsaceus Astragalus karamasicus Astragalus lycius Astragalus sigmoideus Astragalus subsp Astragalus xylobasis angustus biomarker Biomarkers Butanol Carbon 13 Chloroform d‐pinitol endemic Endemic species Fingerprinting Gas chromatography Gas Chromatography-Mass Spectrometry Imidazoline Linolenic acid Magnetic Resonance Spectroscopy Mass spectrometry Mass spectroscopy Metabolomics Methanol NMR Nuclear magnetic resonance Palmitic acid Phenols Plant Extracts Polysaccharides Saccharides Saponins Selenium Toxins Turkey |
| title | Metabolomics of endemic six Astragalus species by combined NMR and GC‐MS analysis |
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