FISH versus real-time quantitative PCR for monitoring of minimal residual disease in chronic myeloid leukaemia patients on tyrosine kinase inhibitor therapy
BCR-ABL fusion gene, the oncogenic driver of CML, results from a translocation between short arms of chromosome 9 and 22. Monitoring of CML patients during treatment is essential, not only for tailoring the treatment but also to detect early relapse to enable timely intervention. Commonly used metho...
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| Veröffentlicht in: | Malaysian journal of pathology 2019-08, Vol.41 (2), p.149-160 |
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| container_title | Malaysian journal of pathology |
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| creator | Haidary, A M Azma, R Z Ithnin, A Alauddin, H Tumian, N R Tamil, A M Razak, N F A Abu Amis, S H Zin, N M Shuib, S |
| description | BCR-ABL fusion gene, the oncogenic driver of CML, results from a translocation between short arms of chromosome 9 and 22. Monitoring of CML patients during treatment is essential, not only for tailoring the treatment but also to detect early relapse to enable timely intervention. Commonly used methods for detection of residual disease are conventional karyotyping, FISH and molecular methods. In this study, we compared FISH with QRT-PCR for detection of residual disease in CML.
CML patients on tyrosine kinase inhibitor (TKI) therapy and on regular follow up at University Kebangsaan Malaysia Medical Center (UKMMC) were selected. A comparative study was conducted between FISH and QRT-PCR for BCR-ABL transcripts at diagnosis and during follow-up.
There was good correlation between FISH and QRT-PCR for BCR-ABL. At 6th month of follow-up post diagnosis, FISH had a sensitivity of 83.3% and specificity of 65.2% (k >0.339, p0.286, p 0.642, p |
| format | Article |
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CML patients on tyrosine kinase inhibitor (TKI) therapy and on regular follow up at University Kebangsaan Malaysia Medical Center (UKMMC) were selected. A comparative study was conducted between FISH and QRT-PCR for BCR-ABL transcripts at diagnosis and during follow-up.
There was good correlation between FISH and QRT-PCR for BCR-ABL. At 6th month of follow-up post diagnosis, FISH had a sensitivity of 83.3% and specificity of 65.2% (k >0.339, p<0.033). At 12th month, the sensitivity of FISH was 83% and the specificity was 59.1% (k >0.286, p <0.065). Similarly, at the 24th month, FISH had a sensitivity of 100% and specificity of 68.8% (k >0.642, p<0.000).
Early achievement of major molecular response (MMR) and complete cytogenetic remission (CCyR) were reliable predictors of long-term maintenance of molecular remission.</description><identifier>ISSN: 0126-8635</identifier><identifier>PMID: 31427550</identifier><language>eng</language><publisher>Malaysia: College of Pathologists, Academy of Medicine of Malaysia</publisher><subject>Adult ; Aged ; Aged, 80 and over ; Antineoplastic Agents - therapeutic use ; Bone marrow ; Chromosomes ; Cytogenetics ; Female ; Fusion Proteins, bcr-abl - analysis ; Humans ; In Situ Hybridization, Fluorescence - methods ; Leukemia ; Leukemia, Myelogenous, Chronic, BCR-ABL Positive - diagnosis ; Leukemia, Myelogenous, Chronic, BCR-ABL Positive - drug therapy ; Male ; Middle Aged ; Neoplasm, Residual ; Polymerase chain reaction ; Protein Kinase Inhibitors - therapeutic use ; Real-Time Polymerase Chain Reaction - methods ; Sensitivity and Specificity ; Stem cell transplantation ; Stem cells ; Studies ; Young Adult</subject><ispartof>Malaysian journal of pathology, 2019-08, Vol.41 (2), p.149-160</ispartof><rights>Copyright College of Pathologists, Academy of Medicine of Malaysia Aug 2019</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31427550$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Haidary, A M</creatorcontrib><creatorcontrib>Azma, R Z</creatorcontrib><creatorcontrib>Ithnin, A</creatorcontrib><creatorcontrib>Alauddin, H</creatorcontrib><creatorcontrib>Tumian, N R</creatorcontrib><creatorcontrib>Tamil, A M</creatorcontrib><creatorcontrib>Razak, N F A</creatorcontrib><creatorcontrib>Abu Amis, S H</creatorcontrib><creatorcontrib>Zin, N M</creatorcontrib><creatorcontrib>Shuib, S</creatorcontrib><title>FISH versus real-time quantitative PCR for monitoring of minimal residual disease in chronic myeloid leukaemia patients on tyrosine kinase inhibitor therapy</title><title>Malaysian journal of pathology</title><addtitle>Malays J Pathol</addtitle><description>BCR-ABL fusion gene, the oncogenic driver of CML, results from a translocation between short arms of chromosome 9 and 22. Monitoring of CML patients during treatment is essential, not only for tailoring the treatment but also to detect early relapse to enable timely intervention. Commonly used methods for detection of residual disease are conventional karyotyping, FISH and molecular methods. In this study, we compared FISH with QRT-PCR for detection of residual disease in CML.
CML patients on tyrosine kinase inhibitor (TKI) therapy and on regular follow up at University Kebangsaan Malaysia Medical Center (UKMMC) were selected. A comparative study was conducted between FISH and QRT-PCR for BCR-ABL transcripts at diagnosis and during follow-up.
There was good correlation between FISH and QRT-PCR for BCR-ABL. At 6th month of follow-up post diagnosis, FISH had a sensitivity of 83.3% and specificity of 65.2% (k >0.339, p<0.033). At 12th month, the sensitivity of FISH was 83% and the specificity was 59.1% (k >0.286, p <0.065). Similarly, at the 24th month, FISH had a sensitivity of 100% and specificity of 68.8% (k >0.642, p<0.000).
Early achievement of major molecular response (MMR) and complete cytogenetic remission (CCyR) were reliable predictors of long-term maintenance of molecular remission.</description><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Antineoplastic Agents - therapeutic use</subject><subject>Bone marrow</subject><subject>Chromosomes</subject><subject>Cytogenetics</subject><subject>Female</subject><subject>Fusion Proteins, bcr-abl - analysis</subject><subject>Humans</subject><subject>In Situ Hybridization, Fluorescence - methods</subject><subject>Leukemia</subject><subject>Leukemia, Myelogenous, Chronic, BCR-ABL Positive - diagnosis</subject><subject>Leukemia, Myelogenous, Chronic, BCR-ABL Positive - drug therapy</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Neoplasm, Residual</subject><subject>Polymerase chain reaction</subject><subject>Protein Kinase Inhibitors - therapeutic use</subject><subject>Real-Time Polymerase Chain Reaction - methods</subject><subject>Sensitivity and Specificity</subject><subject>Stem cell transplantation</subject><subject>Stem cells</subject><subject>Studies</subject><subject>Young Adult</subject><issn>0126-8635</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNpdkEtLw0AUhbNQbK3-Bbngxk1gnnkspVhbKCg-1mGS3Nhpk5l0ZlLof_HHGmnduDp38Z1zD-cimhLKkjhLuJxE195vCUlEnmdX0YRTwVIpyTT6Xqzel3BA5wcPDlUbB90h7Adlgg4q6APC6_wNGuugs0YH67T5AttAp43uVDuavK6H8ai1R-URtIFq40a2gu6IrdU1tDjsFHZaQT9GogkerIFwdNZrg7DT5mTc6PL3A4QNOtUfb6LLRrUeb886iz4XTx_zZbx-eV7NH9dxz3geYtoQFKJmJdalYooQykvCSiq5ohU2eUPyVIqaZEqVnMmyoawRJK1TlqWVyBM-ix5Oub2z-wF9KDrtK2xbZdAOvmCcJ3lGSZKN6P0_dGsHZ8Z2BWMZEVJSRkfq7kwNZYd10btxKncs_nbnP43HgK8</recordid><startdate>201908</startdate><enddate>201908</enddate><creator>Haidary, A M</creator><creator>Azma, R Z</creator><creator>Ithnin, A</creator><creator>Alauddin, H</creator><creator>Tumian, N R</creator><creator>Tamil, A M</creator><creator>Razak, N F A</creator><creator>Abu Amis, S H</creator><creator>Zin, N M</creator><creator>Shuib, S</creator><general>College of Pathologists, Academy of Medicine of Malaysia</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>BVBZV</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>201908</creationdate><title>FISH versus real-time quantitative PCR for monitoring of minimal residual disease in chronic myeloid leukaemia patients on tyrosine kinase inhibitor therapy</title><author>Haidary, A M ; Azma, R Z ; Ithnin, A ; Alauddin, H ; Tumian, N R ; Tamil, A M ; Razak, N F A ; Abu Amis, S H ; Zin, N M ; Shuib, S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p239t-1f0e44d2bedba2a0013b02b153a1cef9f09754d08aab325bf12f407d7287c4963</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Antineoplastic Agents - therapeutic use</topic><topic>Bone marrow</topic><topic>Chromosomes</topic><topic>Cytogenetics</topic><topic>Female</topic><topic>Fusion Proteins, bcr-abl - analysis</topic><topic>Humans</topic><topic>In Situ Hybridization, Fluorescence - methods</topic><topic>Leukemia</topic><topic>Leukemia, Myelogenous, Chronic, BCR-ABL Positive - diagnosis</topic><topic>Leukemia, Myelogenous, Chronic, BCR-ABL Positive - drug therapy</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Neoplasm, Residual</topic><topic>Polymerase chain reaction</topic><topic>Protein Kinase Inhibitors - therapeutic use</topic><topic>Real-Time Polymerase Chain Reaction - methods</topic><topic>Sensitivity and Specificity</topic><topic>Stem cell transplantation</topic><topic>Stem cells</topic><topic>Studies</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Haidary, A M</creatorcontrib><creatorcontrib>Azma, R Z</creatorcontrib><creatorcontrib>Ithnin, A</creatorcontrib><creatorcontrib>Alauddin, H</creatorcontrib><creatorcontrib>Tumian, N R</creatorcontrib><creatorcontrib>Tamil, A M</creatorcontrib><creatorcontrib>Razak, N F A</creatorcontrib><creatorcontrib>Abu Amis, S H</creatorcontrib><creatorcontrib>Zin, N M</creatorcontrib><creatorcontrib>Shuib, S</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>East & South Asia Database</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Malaysian journal of pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Haidary, A M</au><au>Azma, R Z</au><au>Ithnin, A</au><au>Alauddin, H</au><au>Tumian, N R</au><au>Tamil, A M</au><au>Razak, N F A</au><au>Abu Amis, S H</au><au>Zin, N M</au><au>Shuib, S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>FISH versus real-time quantitative PCR for monitoring of minimal residual disease in chronic myeloid leukaemia patients on tyrosine kinase inhibitor therapy</atitle><jtitle>Malaysian journal of pathology</jtitle><addtitle>Malays J Pathol</addtitle><date>2019-08</date><risdate>2019</risdate><volume>41</volume><issue>2</issue><spage>149</spage><epage>160</epage><pages>149-160</pages><issn>0126-8635</issn><abstract>BCR-ABL fusion gene, the oncogenic driver of CML, results from a translocation between short arms of chromosome 9 and 22. Monitoring of CML patients during treatment is essential, not only for tailoring the treatment but also to detect early relapse to enable timely intervention. Commonly used methods for detection of residual disease are conventional karyotyping, FISH and molecular methods. In this study, we compared FISH with QRT-PCR for detection of residual disease in CML.
CML patients on tyrosine kinase inhibitor (TKI) therapy and on regular follow up at University Kebangsaan Malaysia Medical Center (UKMMC) were selected. A comparative study was conducted between FISH and QRT-PCR for BCR-ABL transcripts at diagnosis and during follow-up.
There was good correlation between FISH and QRT-PCR for BCR-ABL. At 6th month of follow-up post diagnosis, FISH had a sensitivity of 83.3% and specificity of 65.2% (k >0.339, p<0.033). At 12th month, the sensitivity of FISH was 83% and the specificity was 59.1% (k >0.286, p <0.065). Similarly, at the 24th month, FISH had a sensitivity of 100% and specificity of 68.8% (k >0.642, p<0.000).
Early achievement of major molecular response (MMR) and complete cytogenetic remission (CCyR) were reliable predictors of long-term maintenance of molecular remission.</abstract><cop>Malaysia</cop><pub>College of Pathologists, Academy of Medicine of Malaysia</pub><pmid>31427550</pmid><tpages>12</tpages></addata></record> |
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| subjects | Adult Aged Aged, 80 and over Antineoplastic Agents - therapeutic use Bone marrow Chromosomes Cytogenetics Female Fusion Proteins, bcr-abl - analysis Humans In Situ Hybridization, Fluorescence - methods Leukemia Leukemia, Myelogenous, Chronic, BCR-ABL Positive - diagnosis Leukemia, Myelogenous, Chronic, BCR-ABL Positive - drug therapy Male Middle Aged Neoplasm, Residual Polymerase chain reaction Protein Kinase Inhibitors - therapeutic use Real-Time Polymerase Chain Reaction - methods Sensitivity and Specificity Stem cell transplantation Stem cells Studies Young Adult |
| title | FISH versus real-time quantitative PCR for monitoring of minimal residual disease in chronic myeloid leukaemia patients on tyrosine kinase inhibitor therapy |
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