Catalytic mechanism and evolutionary characteristics of thioredoxin from Halobacterium salinarum NRC‐1

Catalytic mechanism and evolutionary characteristics of thioredoxin from Halobacterium salinarum NRC‐1

Thioredoxin (TRX) is an important antioxidant against oxidative stress. TRX from the extremely halophilic archaeon Halobacterium salinarum NRC‐1 (HsTRX‐A), which has the highest acidic residue content [(Asp + Glu)/(Arg + Lys + His) = 9.0] among known TRXs, was chosen to elucidate the catalytic mecha...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Acta crystallographica. Section D, Biological crystallography. Biological crystallography., 2020-01, Vol.76 (1), p.73-84
Hauptverfasser: Arai, Shigeki, Shibazaki, Chie, Shimizu, Rumi, Adachi, Motoyasu, Ishibashi, Matsujiro, Tokunaga, Hiroko, Tokunaga, Masao
Format: Artikel
Sprache:eng
Schlagworte:
Antioxidants
Biotechnology
Crystallography
Dichroism
Elongation
Enzymatic activity
Halobacterium salinarum
Halobacterium salinarum NRC‐1
halophile
Homology
Molecular evolution
Mutants
Oxidative stress
Sodium chloride
Structural stability
Substrates
Thioredoxin
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Thioredoxin (TRX) is an important antioxidant against oxidative stress. TRX from the extremely halophilic archaeon Halobacterium salinarum NRC‐1 (HsTRX‐A), which has the highest acidic residue content [(Asp + Glu)/(Arg + Lys + His) = 9.0] among known TRXs, was chosen to elucidate the catalytic mechanism and evolutionary characteristics associated with haloadaptation. X‐ray crystallographic analysis revealed that the main‐chain structure of HsTRX‐A is similar to those of homologous TRXs; for example, the root‐mean‐square deviations on Cα atoms were
ISSN:2059-7983
0907-4449
2059-7983
1399-0047
DOI:10.1107/S2059798319015894