The truncated E protein of DTMUV provide protection in young ducks
•C-terminally truncated E protein, prM-E and E proteins were constructed and applied as subunit vaccine candidates.•The constructed proteins could induce specific antibody responses and provide full protection against DTMUV infection.•The truncated E protein was suggested to be able to serve as a pr...
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| Veröffentlicht in: | Veterinary microbiology 2020-01, Vol.240, p.108508-108508, Article 108508 |
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| container_end_page | 108508 |
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| container_issue | |
| container_start_page | 108508 |
| container_title | Veterinary microbiology |
| container_volume | 240 |
| creator | Li, Linlin Zhang, Yun Dong, Jiawen Zhang, Junqing Zhang, Chunhong Sun, Minhua Cao, Yongchang |
| description | •C-terminally truncated E protein, prM-E and E proteins were constructed and applied as subunit vaccine candidates.•The constructed proteins could induce specific antibody responses and provide full protection against DTMUV infection.•The truncated E protein was suggested to be able to serve as a practical subunit vaccine candidate.
Duck Tembusu virus (DTMUV) is a major pathogen of duck industry in China. In the current study, we generated different constructs containing envelope (E) protein, pre-membrane-envelope (prM-E) protein, and C-terminally truncated E protein of the DTMUV. The constructed proteins could induce specific antibody responses in young ducks. When ducklings were immunized with the constructed proteins, they were 100% protected against DTMUV infection. Furthermore, the fluorescent signal of the truncated E protein was stronger than other constructed proteins, when Bac-to-Bac baculovirus expression system was applied. Our data demonstrated that the truncated E protein used in the current study could be applied as a potential vaccine candidate to control DTMUV infection in young ducks. |
| doi_str_mv | 10.1016/j.vetmic.2019.108508 |
| format | Article |
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Duck Tembusu virus (DTMUV) is a major pathogen of duck industry in China. In the current study, we generated different constructs containing envelope (E) protein, pre-membrane-envelope (prM-E) protein, and C-terminally truncated E protein of the DTMUV. The constructed proteins could induce specific antibody responses in young ducks. When ducklings were immunized with the constructed proteins, they were 100% protected against DTMUV infection. Furthermore, the fluorescent signal of the truncated E protein was stronger than other constructed proteins, when Bac-to-Bac baculovirus expression system was applied. Our data demonstrated that the truncated E protein used in the current study could be applied as a potential vaccine candidate to control DTMUV infection in young ducks.</description><identifier>ISSN: 0378-1135</identifier><identifier>EISSN: 1873-2542</identifier><identifier>DOI: 10.1016/j.vetmic.2019.108508</identifier><identifier>PMID: 31902493</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Aquatic birds ; Baculovirus ; Duck Tembusu virus (DTMUV) ; Envelope (E) protein ; Infections ; Membrane proteins ; Proteins ; Truncated E protein ; Vaccines ; Viral envelope proteins ; Viruses</subject><ispartof>Veterinary microbiology, 2020-01, Vol.240, p.108508-108508, Article 108508</ispartof><rights>2019 Elsevier B.V.</rights><rights>Copyright © 2019 Elsevier B.V. All rights reserved.</rights><rights>Copyright Elsevier BV Jan 2020</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c390t-c3e95077fe5dfd856968296bf2e8363e8dc685dcc728b9c876fe01197141cc8f3</citedby><cites>FETCH-LOGICAL-c390t-c3e95077fe5dfd856968296bf2e8363e8dc685dcc728b9c876fe01197141cc8f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.vetmic.2019.108508$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3549,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31902493$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Linlin</creatorcontrib><creatorcontrib>Zhang, Yun</creatorcontrib><creatorcontrib>Dong, Jiawen</creatorcontrib><creatorcontrib>Zhang, Junqing</creatorcontrib><creatorcontrib>Zhang, Chunhong</creatorcontrib><creatorcontrib>Sun, Minhua</creatorcontrib><creatorcontrib>Cao, Yongchang</creatorcontrib><title>The truncated E protein of DTMUV provide protection in young ducks</title><title>Veterinary microbiology</title><addtitle>Vet Microbiol</addtitle><description>•C-terminally truncated E protein, prM-E and E proteins were constructed and applied as subunit vaccine candidates.•The constructed proteins could induce specific antibody responses and provide full protection against DTMUV infection.•The truncated E protein was suggested to be able to serve as a practical subunit vaccine candidate.
Duck Tembusu virus (DTMUV) is a major pathogen of duck industry in China. In the current study, we generated different constructs containing envelope (E) protein, pre-membrane-envelope (prM-E) protein, and C-terminally truncated E protein of the DTMUV. The constructed proteins could induce specific antibody responses in young ducks. When ducklings were immunized with the constructed proteins, they were 100% protected against DTMUV infection. Furthermore, the fluorescent signal of the truncated E protein was stronger than other constructed proteins, when Bac-to-Bac baculovirus expression system was applied. Our data demonstrated that the truncated E protein used in the current study could be applied as a potential vaccine candidate to control DTMUV infection in young ducks.</description><subject>Aquatic birds</subject><subject>Baculovirus</subject><subject>Duck Tembusu virus (DTMUV)</subject><subject>Envelope (E) protein</subject><subject>Infections</subject><subject>Membrane proteins</subject><subject>Proteins</subject><subject>Truncated E protein</subject><subject>Vaccines</subject><subject>Viral envelope proteins</subject><subject>Viruses</subject><issn>0378-1135</issn><issn>1873-2542</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp9kDtPwzAQgC0EoqXwDxCKxMKS4kfi2AsSlPKQilhaViu1L-DQJMVOKvXf4yqFgYHlTrr77nz-EDoneEww4dfleANtZfWYYiJDSaRYHKAhERmLaZrQQzTELBMxISwdoBPvS4xxIjk-RgNGJKaJZEN0N_-AqHVdrfMWTDSN1q5pwdZRU0T385fF266wsQb6hm5tU0ehvW26-j0ynf70p-ioyFcezvZ5hBYP0_nkKZ69Pj5PbmexZhK3IYJMcZYVkJrCiJRLLqjky4KCYJyBMJqL1GidUbGUWmS8AEyIzEhCtBYFG6Grfm-45KsD36rKeg2rVV5D03lFGWOSCklJQC__oGXTuTpcF6gUMy54-P0IJT2lXeO9g0Ktna1yt1UEq51jVaresdo5Vr3jMHaxX94tKzC_Qz9SA3DTAxBsbCw45bWFWoOxLhhUprH_v_ANCNmNBA</recordid><startdate>202001</startdate><enddate>202001</enddate><creator>Li, Linlin</creator><creator>Zhang, Yun</creator><creator>Dong, Jiawen</creator><creator>Zhang, Junqing</creator><creator>Zhang, Chunhong</creator><creator>Sun, Minhua</creator><creator>Cao, Yongchang</creator><general>Elsevier B.V</general><general>Elsevier BV</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>202001</creationdate><title>The truncated E protein of DTMUV provide protection in young ducks</title><author>Li, Linlin ; Zhang, Yun ; Dong, Jiawen ; Zhang, Junqing ; Zhang, Chunhong ; Sun, Minhua ; Cao, Yongchang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c390t-c3e95077fe5dfd856968296bf2e8363e8dc685dcc728b9c876fe01197141cc8f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Aquatic birds</topic><topic>Baculovirus</topic><topic>Duck Tembusu virus (DTMUV)</topic><topic>Envelope (E) protein</topic><topic>Infections</topic><topic>Membrane proteins</topic><topic>Proteins</topic><topic>Truncated E protein</topic><topic>Vaccines</topic><topic>Viral envelope proteins</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Linlin</creatorcontrib><creatorcontrib>Zhang, Yun</creatorcontrib><creatorcontrib>Dong, Jiawen</creatorcontrib><creatorcontrib>Zhang, Junqing</creatorcontrib><creatorcontrib>Zhang, Chunhong</creatorcontrib><creatorcontrib>Sun, Minhua</creatorcontrib><creatorcontrib>Cao, Yongchang</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Linlin</au><au>Zhang, Yun</au><au>Dong, Jiawen</au><au>Zhang, Junqing</au><au>Zhang, Chunhong</au><au>Sun, Minhua</au><au>Cao, Yongchang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The truncated E protein of DTMUV provide protection in young ducks</atitle><jtitle>Veterinary microbiology</jtitle><addtitle>Vet Microbiol</addtitle><date>2020-01</date><risdate>2020</risdate><volume>240</volume><spage>108508</spage><epage>108508</epage><pages>108508-108508</pages><artnum>108508</artnum><issn>0378-1135</issn><eissn>1873-2542</eissn><abstract>•C-terminally truncated E protein, prM-E and E proteins were constructed and applied as subunit vaccine candidates.•The constructed proteins could induce specific antibody responses and provide full protection against DTMUV infection.•The truncated E protein was suggested to be able to serve as a practical subunit vaccine candidate.
Duck Tembusu virus (DTMUV) is a major pathogen of duck industry in China. In the current study, we generated different constructs containing envelope (E) protein, pre-membrane-envelope (prM-E) protein, and C-terminally truncated E protein of the DTMUV. The constructed proteins could induce specific antibody responses in young ducks. When ducklings were immunized with the constructed proteins, they were 100% protected against DTMUV infection. Furthermore, the fluorescent signal of the truncated E protein was stronger than other constructed proteins, when Bac-to-Bac baculovirus expression system was applied. Our data demonstrated that the truncated E protein used in the current study could be applied as a potential vaccine candidate to control DTMUV infection in young ducks.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>31902493</pmid><doi>10.1016/j.vetmic.2019.108508</doi><tpages>1</tpages></addata></record> |
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| ispartof | Veterinary microbiology, 2020-01, Vol.240, p.108508-108508, Article 108508 |
| issn | 0378-1135 1873-2542 |
| language | eng |
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| source | ScienceDirect Journals (5 years ago - present) |
| subjects | Aquatic birds Baculovirus Duck Tembusu virus (DTMUV) Envelope (E) protein Infections Membrane proteins Proteins Truncated E protein Vaccines Viral envelope proteins Viruses |
| title | The truncated E protein of DTMUV provide protection in young ducks |
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