Total triterpenoids from the fruits of Chaenomeles speciosa exerted gastroprotective activities on indomethacin-induced gastric damage via modulating microRNA-423-5p-mediated TFF/NAG-1 and apoptotic pathways
Our previous studies have demonstrated that the total triterpenes from the fruits of Chaenomeles speciosa (CSTT) exhibit effective therapeutic effects on gastric ulcer patients and animals. The present aim is to further investigate the mechanisms involved. The results indicated that CSTT could ameli...
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| Veröffentlicht in: | Food & function 2020-01, Vol.11 (1), p.662-679 |
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| creator | He, Haibo Feng, Minlu Xu, Haiyan Li, Xiaomei He, Yumin Qin, Huilin Zhang, Yongfeng Tang, Hongbo Zou, Kun |
| description | Our previous studies have demonstrated that the total triterpenes from the fruits of Chaenomeles speciosa (CSTT) exhibit effective therapeutic effects on gastric ulcer patients and animals. The present aim is to further investigate the mechanisms involved. The results indicated that CSTT could ameliorate IND-induced gastric injury, which was related to promoting IND-damaged GES-1 cell proliferation and migration, improving the IND-damaged rat GBF, ulcer area, inhibition rate and pathologic changes of gastric mucous tissue, increasing the amount of adhered gastric mucus, attenuating the volume and total acidity of the gastric effluents, and augmenting the gastric pH; further studies showed that CSTT obviously downregulated miR-423-5p mRNA, NAG-1 mRNA and protein expression, Bax, Bad, cytosol cytochrome C, Apaf-1, cleaved-caspase-3, and cleaved-caspase-9 protein expression and cytosol cytochrome C concentration, and upregulated TFF1, TFF2 and TFF3 mRNA and protein expression, Bcl-2, Bcl-xl, pro-caspase-3, and pro-caspase-9 protein expression, mitochondrial viability, mitochondrial cytochrome C concentration and Bcl-2/Bax, Bcl-xl/Bad ratios. These findings demonstrated that CSTT protected against IND-induced gastric damage by depressing miR-423-5p expression and modulating the TFF/NAG-1 pathway, which in turn restrained mitochondrion-mediated apoptosis. |
| doi_str_mv | 10.1039/c9fo02322d |
| format | Article |
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The present aim is to further investigate the mechanisms involved. The results indicated that CSTT could ameliorate IND-induced gastric injury, which was related to promoting IND-damaged GES-1 cell proliferation and migration, improving the IND-damaged rat GBF, ulcer area, inhibition rate and pathologic changes of gastric mucous tissue, increasing the amount of adhered gastric mucus, attenuating the volume and total acidity of the gastric effluents, and augmenting the gastric pH; further studies showed that CSTT obviously downregulated miR-423-5p mRNA, NAG-1 mRNA and protein expression, Bax, Bad, cytosol cytochrome C, Apaf-1, cleaved-caspase-3, and cleaved-caspase-9 protein expression and cytosol cytochrome C concentration, and upregulated TFF1, TFF2 and TFF3 mRNA and protein expression, Bcl-2, Bcl-xl, pro-caspase-3, and pro-caspase-9 protein expression, mitochondrial viability, mitochondrial cytochrome C concentration and Bcl-2/Bax, Bcl-xl/Bad ratios. These findings demonstrated that CSTT protected against IND-induced gastric damage by depressing miR-423-5p expression and modulating the TFF/NAG-1 pathway, which in turn restrained mitochondrion-mediated apoptosis.</description><identifier>ISSN: 2042-6496</identifier><identifier>EISSN: 2042-650X</identifier><identifier>DOI: 10.1039/c9fo02322d</identifier><identifier>PMID: 31895380</identifier><language>eng</language><publisher>England: Royal Society of Chemistry</publisher><subject>Acidity ; Apaf-1 protein ; Apoptosis ; Bax protein ; Bcl-2 protein ; Bcl-x protein ; Caspase-3 ; Caspase-9 ; Cell migration ; Cell proliferation ; Chaenomeles speciosa ; Cytochrome ; Cytochrome c ; Cytochromes ; Cytosol ; Damage ; Fruits ; Gene expression ; Indomethacin ; miRNA ; Mitochondria ; mRNA ; Mucus ; Protein expression ; Proteins ; Ribonucleic acid ; RNA ; Triterpenes ; Triterpenoids ; Ulcers ; Viability</subject><ispartof>Food & function, 2020-01, Vol.11 (1), p.662-679</ispartof><rights>Copyright Royal Society of Chemistry 2020</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-843aecf6e8bc7ca66ce459e307c0234a57cc0473527b6f7dbcdfa835773827763</citedby><cites>FETCH-LOGICAL-c356t-843aecf6e8bc7ca66ce459e307c0234a57cc0473527b6f7dbcdfa835773827763</cites><orcidid>0000-0001-6606-2624 ; 0000-0003-3048-8353 ; 0000-0001-5182-6855 ; 0000-0002-2517-3165 ; 0000-0002-7339-3242 ; 0000-0002-8478-5313 ; 0000-0002-6798-832X ; 0000-0002-9717-5311 ; 0000-0001-9109-9090</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31895380$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>He, Haibo</creatorcontrib><creatorcontrib>Feng, Minlu</creatorcontrib><creatorcontrib>Xu, Haiyan</creatorcontrib><creatorcontrib>Li, Xiaomei</creatorcontrib><creatorcontrib>He, Yumin</creatorcontrib><creatorcontrib>Qin, Huilin</creatorcontrib><creatorcontrib>Zhang, Yongfeng</creatorcontrib><creatorcontrib>Tang, Hongbo</creatorcontrib><creatorcontrib>Zou, Kun</creatorcontrib><title>Total triterpenoids from the fruits of Chaenomeles speciosa exerted gastroprotective activities on indomethacin-induced gastric damage via modulating microRNA-423-5p-mediated TFF/NAG-1 and apoptotic pathways</title><title>Food & function</title><addtitle>Food Funct</addtitle><description>Our previous studies have demonstrated that the total triterpenes from the fruits of Chaenomeles speciosa (CSTT) exhibit effective therapeutic effects on gastric ulcer patients and animals. The present aim is to further investigate the mechanisms involved. The results indicated that CSTT could ameliorate IND-induced gastric injury, which was related to promoting IND-damaged GES-1 cell proliferation and migration, improving the IND-damaged rat GBF, ulcer area, inhibition rate and pathologic changes of gastric mucous tissue, increasing the amount of adhered gastric mucus, attenuating the volume and total acidity of the gastric effluents, and augmenting the gastric pH; further studies showed that CSTT obviously downregulated miR-423-5p mRNA, NAG-1 mRNA and protein expression, Bax, Bad, cytosol cytochrome C, Apaf-1, cleaved-caspase-3, and cleaved-caspase-9 protein expression and cytosol cytochrome C concentration, and upregulated TFF1, TFF2 and TFF3 mRNA and protein expression, Bcl-2, Bcl-xl, pro-caspase-3, and pro-caspase-9 protein expression, mitochondrial viability, mitochondrial cytochrome C concentration and Bcl-2/Bax, Bcl-xl/Bad ratios. These findings demonstrated that CSTT protected against IND-induced gastric damage by depressing miR-423-5p expression and modulating the TFF/NAG-1 pathway, which in turn restrained mitochondrion-mediated apoptosis.</description><subject>Acidity</subject><subject>Apaf-1 protein</subject><subject>Apoptosis</subject><subject>Bax protein</subject><subject>Bcl-2 protein</subject><subject>Bcl-x protein</subject><subject>Caspase-3</subject><subject>Caspase-9</subject><subject>Cell migration</subject><subject>Cell proliferation</subject><subject>Chaenomeles speciosa</subject><subject>Cytochrome</subject><subject>Cytochrome c</subject><subject>Cytochromes</subject><subject>Cytosol</subject><subject>Damage</subject><subject>Fruits</subject><subject>Gene expression</subject><subject>Indomethacin</subject><subject>miRNA</subject><subject>Mitochondria</subject><subject>mRNA</subject><subject>Mucus</subject><subject>Protein expression</subject><subject>Proteins</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>Triterpenes</subject><subject>Triterpenoids</subject><subject>Ulcers</subject><subject>Viability</subject><issn>2042-6496</issn><issn>2042-650X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNpdkcFq3DAQhkVpaUKaSx-gCHopBTeyxrbs47LtpoWQQNhAbmZWGu8q2JYryWnylHmlapNsD9VlRuibfzTzM_YxF99yAc2ZbjonJEhp3rBjKQqZVaW4fXvIi6Y6Yqch3Il0oGnqpn7PjiCvmxJqccye1i5iz6O3kfxEo7Mm8M67gccdpWS2MXDX8eUO0-NAPQUeJtLWBeT0QD6S4VsM0bvJu0g62nviuA822gS7kdvRpMq4Q23HLF1mfaixmhsccEv83iIfnJl7jHbc8sFq764vF1khISunbCBjcd9qvVqdXS7Os5zjaDhOboouJpkJ4-4PPoYP7F2HfaDT13jCblY_1suf2cXV-a_l4iLTUFYxqwtA0l1F9UYrjVWlqSgbAqF0WmaBpdJaFApKqTZVp8xGmw5rKJWCWipVwQn78qKbpv49U4jtYIOmvseR3BxaCSBFnedQJPTzf-idm_2YfpeoQkkloYREfX2h0uAheOraydsB_WObi3bvdLtsVlfPTn9P8KdXyXmTVvMPPfgKfwElO6cv</recordid><startdate>20200129</startdate><enddate>20200129</enddate><creator>He, Haibo</creator><creator>Feng, Minlu</creator><creator>Xu, Haiyan</creator><creator>Li, Xiaomei</creator><creator>He, Yumin</creator><creator>Qin, Huilin</creator><creator>Zhang, Yongfeng</creator><creator>Tang, Hongbo</creator><creator>Zou, Kun</creator><general>Royal Society of Chemistry</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7T7</scope><scope>7TO</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-6606-2624</orcidid><orcidid>https://orcid.org/0000-0003-3048-8353</orcidid><orcidid>https://orcid.org/0000-0001-5182-6855</orcidid><orcidid>https://orcid.org/0000-0002-2517-3165</orcidid><orcidid>https://orcid.org/0000-0002-7339-3242</orcidid><orcidid>https://orcid.org/0000-0002-8478-5313</orcidid><orcidid>https://orcid.org/0000-0002-6798-832X</orcidid><orcidid>https://orcid.org/0000-0002-9717-5311</orcidid><orcidid>https://orcid.org/0000-0001-9109-9090</orcidid></search><sort><creationdate>20200129</creationdate><title>Total triterpenoids from the fruits of Chaenomeles speciosa exerted gastroprotective activities on indomethacin-induced gastric damage via modulating microRNA-423-5p-mediated TFF/NAG-1 and apoptotic pathways</title><author>He, Haibo ; Feng, Minlu ; Xu, Haiyan ; Li, Xiaomei ; He, Yumin ; Qin, Huilin ; Zhang, Yongfeng ; Tang, Hongbo ; Zou, Kun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-843aecf6e8bc7ca66ce459e307c0234a57cc0473527b6f7dbcdfa835773827763</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Acidity</topic><topic>Apaf-1 protein</topic><topic>Apoptosis</topic><topic>Bax protein</topic><topic>Bcl-2 protein</topic><topic>Bcl-x protein</topic><topic>Caspase-3</topic><topic>Caspase-9</topic><topic>Cell migration</topic><topic>Cell proliferation</topic><topic>Chaenomeles speciosa</topic><topic>Cytochrome</topic><topic>Cytochrome c</topic><topic>Cytochromes</topic><topic>Cytosol</topic><topic>Damage</topic><topic>Fruits</topic><topic>Gene expression</topic><topic>Indomethacin</topic><topic>miRNA</topic><topic>Mitochondria</topic><topic>mRNA</topic><topic>Mucus</topic><topic>Protein expression</topic><topic>Proteins</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>Triterpenes</topic><topic>Triterpenoids</topic><topic>Ulcers</topic><topic>Viability</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>He, Haibo</creatorcontrib><creatorcontrib>Feng, Minlu</creatorcontrib><creatorcontrib>Xu, Haiyan</creatorcontrib><creatorcontrib>Li, Xiaomei</creatorcontrib><creatorcontrib>He, Yumin</creatorcontrib><creatorcontrib>Qin, Huilin</creatorcontrib><creatorcontrib>Zhang, Yongfeng</creatorcontrib><creatorcontrib>Tang, Hongbo</creatorcontrib><creatorcontrib>Zou, Kun</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Food & function</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>He, Haibo</au><au>Feng, Minlu</au><au>Xu, Haiyan</au><au>Li, Xiaomei</au><au>He, Yumin</au><au>Qin, Huilin</au><au>Zhang, Yongfeng</au><au>Tang, Hongbo</au><au>Zou, Kun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Total triterpenoids from the fruits of Chaenomeles speciosa exerted gastroprotective activities on indomethacin-induced gastric damage via modulating microRNA-423-5p-mediated TFF/NAG-1 and apoptotic pathways</atitle><jtitle>Food & function</jtitle><addtitle>Food Funct</addtitle><date>2020-01-29</date><risdate>2020</risdate><volume>11</volume><issue>1</issue><spage>662</spage><epage>679</epage><pages>662-679</pages><issn>2042-6496</issn><eissn>2042-650X</eissn><abstract>Our previous studies have demonstrated that the total triterpenes from the fruits of Chaenomeles speciosa (CSTT) exhibit effective therapeutic effects on gastric ulcer patients and animals. The present aim is to further investigate the mechanisms involved. The results indicated that CSTT could ameliorate IND-induced gastric injury, which was related to promoting IND-damaged GES-1 cell proliferation and migration, improving the IND-damaged rat GBF, ulcer area, inhibition rate and pathologic changes of gastric mucous tissue, increasing the amount of adhered gastric mucus, attenuating the volume and total acidity of the gastric effluents, and augmenting the gastric pH; further studies showed that CSTT obviously downregulated miR-423-5p mRNA, NAG-1 mRNA and protein expression, Bax, Bad, cytosol cytochrome C, Apaf-1, cleaved-caspase-3, and cleaved-caspase-9 protein expression and cytosol cytochrome C concentration, and upregulated TFF1, TFF2 and TFF3 mRNA and protein expression, Bcl-2, Bcl-xl, pro-caspase-3, and pro-caspase-9 protein expression, mitochondrial viability, mitochondrial cytochrome C concentration and Bcl-2/Bax, Bcl-xl/Bad ratios. These findings demonstrated that CSTT protected against IND-induced gastric damage by depressing miR-423-5p expression and modulating the TFF/NAG-1 pathway, which in turn restrained mitochondrion-mediated apoptosis.</abstract><cop>England</cop><pub>Royal Society of Chemistry</pub><pmid>31895380</pmid><doi>10.1039/c9fo02322d</doi><tpages>18</tpages><orcidid>https://orcid.org/0000-0001-6606-2624</orcidid><orcidid>https://orcid.org/0000-0003-3048-8353</orcidid><orcidid>https://orcid.org/0000-0001-5182-6855</orcidid><orcidid>https://orcid.org/0000-0002-2517-3165</orcidid><orcidid>https://orcid.org/0000-0002-7339-3242</orcidid><orcidid>https://orcid.org/0000-0002-8478-5313</orcidid><orcidid>https://orcid.org/0000-0002-6798-832X</orcidid><orcidid>https://orcid.org/0000-0002-9717-5311</orcidid><orcidid>https://orcid.org/0000-0001-9109-9090</orcidid></addata></record> |
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| subjects | Acidity Apaf-1 protein Apoptosis Bax protein Bcl-2 protein Bcl-x protein Caspase-3 Caspase-9 Cell migration Cell proliferation Chaenomeles speciosa Cytochrome Cytochrome c Cytochromes Cytosol Damage Fruits Gene expression Indomethacin miRNA Mitochondria mRNA Mucus Protein expression Proteins Ribonucleic acid RNA Triterpenes Triterpenoids Ulcers Viability |
| title | Total triterpenoids from the fruits of Chaenomeles speciosa exerted gastroprotective activities on indomethacin-induced gastric damage via modulating microRNA-423-5p-mediated TFF/NAG-1 and apoptotic pathways |
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