Production of fructooligosaccharides by Bacillus subtilis natto CCT7712 and their antiproliferative potential
Aims Fructooligosaccharides (FOSs) known for their health properties and β‐(2→6)‐levan‐type FOSs have shown prebiotic and immunomodulatory activities that overcome those of commercial β‐(2→1)‐FOSs, but costs do not favour their use. Moreover, FOSs can reach the bloodstream through the diet, and litt...
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| Veröffentlicht in: | Journal of applied microbiology 2020-05, Vol.128 (5), p.1414-1426 |
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| container_title | Journal of applied microbiology |
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| creator | Magri, A. Oliveira, M.R. Baldo, C. Tischer, C.A. Sartori, D. Mantovani, M.S. Celligoi, M.A.P.C. |
| description | Aims
Fructooligosaccharides (FOSs) known for their health properties and β‐(2→6)‐levan‐type FOSs have shown prebiotic and immunomodulatory activities that overcome those of commercial β‐(2→1)‐FOSs, but costs do not favour their use. Moreover, FOSs can reach the bloodstream through the diet, and little is known about their direct effect on cells. The aim of this work was to produce high‐content FOSs by Bacillus subtilis natto CCT7712 in a bioreactor using commercial sucrose and to evaluate their antiproliferative effects in OVCAR‐3 cells.
Methods and Results
FOS production reached 173·60 g l−1, 0·2 vvm aeration and uncontrolled pH. Levan‐type FOSs, composed of β‐(2 → 6) links and mainly GF3 (6‐nystose), were identified using RMN spectroscopy, FT‐IR and ESI‐MS. FOSs decreased the viability and proliferation of OVCAR‐3 cells, and the effects were associated with an increased pro‐inflammatory response by the induction of IL‐8 and TNF‐α, and the repression of ER‐β genes. The metabolic profiles showed disruption of cellular homeostasis that can be associated with a decrease in proliferation.
Conclusions
The high production of levan‐type FOSs from B. subtilis natto CCT7712 in a bioreactor was achieved, and they showed antiproliferative potential in OVCAR‐3 cells.
Significance and Impact of the Study
FOS could be a good target for future therapeutic studies and commercial use. |
| doi_str_mv | 10.1111/jam.14569 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2331625259</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2331625259</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3539-5a5c0dbedc71bff87656890908c6464c6575e75824a2f1c7c0e778ac566d24073</originalsourceid><addsrcrecordid>eNp1kctKxDAUhoMo3he-gATc6KJOLs2lSx28ouhC1yVNU82QNmOSKvP2xhl1IXg25yd8fBzyA3CA0SnOM5mp_hSXjFdrYBtTzgrCBVlf5rJgSJAtsBPjDCFMEeObYItiWeGSym3QPwbfjjpZP0DfwS7k7L2zLz4qrV9VsK2JsFnAc6Wtc2OEcWySdTbCQaXk4XT6JAQmUA0tTK_GhpySnYfs6ExQyb4bOPfJ5Efl9sBGp1w0-997FzxfXjxNr4u7h6ub6dldoSmjVcEU06htTKsFbrpOCs64rFCFpOYlLzVnghnBJCkV6bAWGhkhpNKM85aUSNBdcLzy5jveRhNT3duojXNqMH6MNaEUc8IIqzJ69Aed-TEM-bpMyYpKJBDL1MmK0sHHGExXz4PtVVjUGNVfHdS5g3rZQWYPv41j05v2l_z59AxMVsCHdWbxv6m-PbtfKT8BWwuQTw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2389380705</pqid></control><display><type>article</type><title>Production of fructooligosaccharides by Bacillus subtilis natto CCT7712 and their antiproliferative potential</title><source>Access via Wiley Online Library</source><source>Oxford University Press Journals All Titles (1996-Current)</source><creator>Magri, A. ; Oliveira, M.R. ; Baldo, C. ; Tischer, C.A. ; Sartori, D. ; Mantovani, M.S. ; Celligoi, M.A.P.C.</creator><creatorcontrib>Magri, A. ; Oliveira, M.R. ; Baldo, C. ; Tischer, C.A. ; Sartori, D. ; Mantovani, M.S. ; Celligoi, M.A.P.C.</creatorcontrib><description>Aims
Fructooligosaccharides (FOSs) known for their health properties and β‐(2→6)‐levan‐type FOSs have shown prebiotic and immunomodulatory activities that overcome those of commercial β‐(2→1)‐FOSs, but costs do not favour their use. Moreover, FOSs can reach the bloodstream through the diet, and little is known about their direct effect on cells. The aim of this work was to produce high‐content FOSs by Bacillus subtilis natto CCT7712 in a bioreactor using commercial sucrose and to evaluate their antiproliferative effects in OVCAR‐3 cells.
Methods and Results
FOS production reached 173·60 g l−1, 0·2 vvm aeration and uncontrolled pH. Levan‐type FOSs, composed of β‐(2 → 6) links and mainly GF3 (6‐nystose), were identified using RMN spectroscopy, FT‐IR and ESI‐MS. FOSs decreased the viability and proliferation of OVCAR‐3 cells, and the effects were associated with an increased pro‐inflammatory response by the induction of IL‐8 and TNF‐α, and the repression of ER‐β genes. The metabolic profiles showed disruption of cellular homeostasis that can be associated with a decrease in proliferation.
Conclusions
The high production of levan‐type FOSs from B. subtilis natto CCT7712 in a bioreactor was achieved, and they showed antiproliferative potential in OVCAR‐3 cells.
Significance and Impact of the Study
FOS could be a good target for future therapeutic studies and commercial use.</description><identifier>ISSN: 1364-5072</identifier><identifier>EISSN: 1365-2672</identifier><identifier>DOI: 10.1111/jam.14569</identifier><identifier>PMID: 31891438</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Aeration ; antiproliferative ; Antiproliferatives ; Bacillus sp ; Bacillus subtilis ; bioreactor ; Bioreactors ; Cell proliferation ; fructooligosaccharide ; Fructooligosaccharides ; Gene silencing ; Homeostasis ; Immunomodulation ; Inflammation ; Inflammatory response ; Infrared spectroscopy ; Levan ; OVCAR‐3 ; Production methods ; Sucrose ; Sugar ; Tumor necrosis factor ; Viability</subject><ispartof>Journal of applied microbiology, 2020-05, Vol.128 (5), p.1414-1426</ispartof><rights>2019 The Society for Applied Microbiology</rights><rights>2019 The Society for Applied Microbiology.</rights><rights>Copyright © 2020 The Society for Applied Microbiology</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3539-5a5c0dbedc71bff87656890908c6464c6575e75824a2f1c7c0e778ac566d24073</citedby><cites>FETCH-LOGICAL-c3539-5a5c0dbedc71bff87656890908c6464c6575e75824a2f1c7c0e778ac566d24073</cites><orcidid>0000-0003-2217-8937 ; 0000-0003-2834-4940</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjam.14569$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjam.14569$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31891438$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Magri, A.</creatorcontrib><creatorcontrib>Oliveira, M.R.</creatorcontrib><creatorcontrib>Baldo, C.</creatorcontrib><creatorcontrib>Tischer, C.A.</creatorcontrib><creatorcontrib>Sartori, D.</creatorcontrib><creatorcontrib>Mantovani, M.S.</creatorcontrib><creatorcontrib>Celligoi, M.A.P.C.</creatorcontrib><title>Production of fructooligosaccharides by Bacillus subtilis natto CCT7712 and their antiproliferative potential</title><title>Journal of applied microbiology</title><addtitle>J Appl Microbiol</addtitle><description>Aims
Fructooligosaccharides (FOSs) known for their health properties and β‐(2→6)‐levan‐type FOSs have shown prebiotic and immunomodulatory activities that overcome those of commercial β‐(2→1)‐FOSs, but costs do not favour their use. Moreover, FOSs can reach the bloodstream through the diet, and little is known about their direct effect on cells. The aim of this work was to produce high‐content FOSs by Bacillus subtilis natto CCT7712 in a bioreactor using commercial sucrose and to evaluate their antiproliferative effects in OVCAR‐3 cells.
Methods and Results
FOS production reached 173·60 g l−1, 0·2 vvm aeration and uncontrolled pH. Levan‐type FOSs, composed of β‐(2 → 6) links and mainly GF3 (6‐nystose), were identified using RMN spectroscopy, FT‐IR and ESI‐MS. FOSs decreased the viability and proliferation of OVCAR‐3 cells, and the effects were associated with an increased pro‐inflammatory response by the induction of IL‐8 and TNF‐α, and the repression of ER‐β genes. The metabolic profiles showed disruption of cellular homeostasis that can be associated with a decrease in proliferation.
Conclusions
The high production of levan‐type FOSs from B. subtilis natto CCT7712 in a bioreactor was achieved, and they showed antiproliferative potential in OVCAR‐3 cells.
Significance and Impact of the Study
FOS could be a good target for future therapeutic studies and commercial use.</description><subject>Aeration</subject><subject>antiproliferative</subject><subject>Antiproliferatives</subject><subject>Bacillus sp</subject><subject>Bacillus subtilis</subject><subject>bioreactor</subject><subject>Bioreactors</subject><subject>Cell proliferation</subject><subject>fructooligosaccharide</subject><subject>Fructooligosaccharides</subject><subject>Gene silencing</subject><subject>Homeostasis</subject><subject>Immunomodulation</subject><subject>Inflammation</subject><subject>Inflammatory response</subject><subject>Infrared spectroscopy</subject><subject>Levan</subject><subject>OVCAR‐3</subject><subject>Production methods</subject><subject>Sucrose</subject><subject>Sugar</subject><subject>Tumor necrosis factor</subject><subject>Viability</subject><issn>1364-5072</issn><issn>1365-2672</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp1kctKxDAUhoMo3he-gATc6KJOLs2lSx28ouhC1yVNU82QNmOSKvP2xhl1IXg25yd8fBzyA3CA0SnOM5mp_hSXjFdrYBtTzgrCBVlf5rJgSJAtsBPjDCFMEeObYItiWeGSym3QPwbfjjpZP0DfwS7k7L2zLz4qrV9VsK2JsFnAc6Wtc2OEcWySdTbCQaXk4XT6JAQmUA0tTK_GhpySnYfs6ExQyb4bOPfJ5Efl9sBGp1w0-997FzxfXjxNr4u7h6ub6dldoSmjVcEU06htTKsFbrpOCs64rFCFpOYlLzVnghnBJCkV6bAWGhkhpNKM85aUSNBdcLzy5jveRhNT3duojXNqMH6MNaEUc8IIqzJ69Aed-TEM-bpMyYpKJBDL1MmK0sHHGExXz4PtVVjUGNVfHdS5g3rZQWYPv41j05v2l_z59AxMVsCHdWbxv6m-PbtfKT8BWwuQTw</recordid><startdate>202005</startdate><enddate>202005</enddate><creator>Magri, A.</creator><creator>Oliveira, M.R.</creator><creator>Baldo, C.</creator><creator>Tischer, C.A.</creator><creator>Sartori, D.</creator><creator>Mantovani, M.S.</creator><creator>Celligoi, M.A.P.C.</creator><general>Oxford University Press</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TM</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-2217-8937</orcidid><orcidid>https://orcid.org/0000-0003-2834-4940</orcidid></search><sort><creationdate>202005</creationdate><title>Production of fructooligosaccharides by Bacillus subtilis natto CCT7712 and their antiproliferative potential</title><author>Magri, A. ; Oliveira, M.R. ; Baldo, C. ; Tischer, C.A. ; Sartori, D. ; Mantovani, M.S. ; Celligoi, M.A.P.C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3539-5a5c0dbedc71bff87656890908c6464c6575e75824a2f1c7c0e778ac566d24073</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Aeration</topic><topic>antiproliferative</topic><topic>Antiproliferatives</topic><topic>Bacillus sp</topic><topic>Bacillus subtilis</topic><topic>bioreactor</topic><topic>Bioreactors</topic><topic>Cell proliferation</topic><topic>fructooligosaccharide</topic><topic>Fructooligosaccharides</topic><topic>Gene silencing</topic><topic>Homeostasis</topic><topic>Immunomodulation</topic><topic>Inflammation</topic><topic>Inflammatory response</topic><topic>Infrared spectroscopy</topic><topic>Levan</topic><topic>OVCAR‐3</topic><topic>Production methods</topic><topic>Sucrose</topic><topic>Sugar</topic><topic>Tumor necrosis factor</topic><topic>Viability</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Magri, A.</creatorcontrib><creatorcontrib>Oliveira, M.R.</creatorcontrib><creatorcontrib>Baldo, C.</creatorcontrib><creatorcontrib>Tischer, C.A.</creatorcontrib><creatorcontrib>Sartori, D.</creatorcontrib><creatorcontrib>Mantovani, M.S.</creatorcontrib><creatorcontrib>Celligoi, M.A.P.C.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Magri, A.</au><au>Oliveira, M.R.</au><au>Baldo, C.</au><au>Tischer, C.A.</au><au>Sartori, D.</au><au>Mantovani, M.S.</au><au>Celligoi, M.A.P.C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Production of fructooligosaccharides by Bacillus subtilis natto CCT7712 and their antiproliferative potential</atitle><jtitle>Journal of applied microbiology</jtitle><addtitle>J Appl Microbiol</addtitle><date>2020-05</date><risdate>2020</risdate><volume>128</volume><issue>5</issue><spage>1414</spage><epage>1426</epage><pages>1414-1426</pages><issn>1364-5072</issn><eissn>1365-2672</eissn><abstract>Aims
Fructooligosaccharides (FOSs) known for their health properties and β‐(2→6)‐levan‐type FOSs have shown prebiotic and immunomodulatory activities that overcome those of commercial β‐(2→1)‐FOSs, but costs do not favour their use. Moreover, FOSs can reach the bloodstream through the diet, and little is known about their direct effect on cells. The aim of this work was to produce high‐content FOSs by Bacillus subtilis natto CCT7712 in a bioreactor using commercial sucrose and to evaluate their antiproliferative effects in OVCAR‐3 cells.
Methods and Results
FOS production reached 173·60 g l−1, 0·2 vvm aeration and uncontrolled pH. Levan‐type FOSs, composed of β‐(2 → 6) links and mainly GF3 (6‐nystose), were identified using RMN spectroscopy, FT‐IR and ESI‐MS. FOSs decreased the viability and proliferation of OVCAR‐3 cells, and the effects were associated with an increased pro‐inflammatory response by the induction of IL‐8 and TNF‐α, and the repression of ER‐β genes. The metabolic profiles showed disruption of cellular homeostasis that can be associated with a decrease in proliferation.
Conclusions
The high production of levan‐type FOSs from B. subtilis natto CCT7712 in a bioreactor was achieved, and they showed antiproliferative potential in OVCAR‐3 cells.
Significance and Impact of the Study
FOS could be a good target for future therapeutic studies and commercial use.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>31891438</pmid><doi>10.1111/jam.14569</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0003-2217-8937</orcidid><orcidid>https://orcid.org/0000-0003-2834-4940</orcidid></addata></record> |
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| source | Access via Wiley Online Library; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Aeration antiproliferative Antiproliferatives Bacillus sp Bacillus subtilis bioreactor Bioreactors Cell proliferation fructooligosaccharide Fructooligosaccharides Gene silencing Homeostasis Immunomodulation Inflammation Inflammatory response Infrared spectroscopy Levan OVCAR‐3 Production methods Sucrose Sugar Tumor necrosis factor Viability |
| title | Production of fructooligosaccharides by Bacillus subtilis natto CCT7712 and their antiproliferative potential |
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