Polyvinyl alcohol modified polyvinylidene fluoride‐graphene oxide scaffold promotes osteogenic differentiation potential of human induced pluripotent stem cells

Tissue engineering is fast becoming a key approach in bone medicine studies. Designing the ideally desirable combination of stem cells and scaffolds are at the hurt of efforts for producing implantable bone substitutes. Clinical application of stem cells could be associated with serious limitations, and engineering scaffolds that are able to imitate the important features of extracellular matrix is a major area of challenges within the field. In this study, electrospun scaffolds of polyvinylidene fluoride (PVDF), PVDF‐graphene oxide (GO), PVDF‐polyvinyl alcohol (PVA) and PVDF‐PVA‐GO were fabricated to study the osteogenic differentiation potential of human induced pluripotent stem cells (iPSCs) while cultured on fabricated scaffolds. Scanning electron microscopy study, viability assay, relative gene expression analysis, immunocytochemistry, alkaline phosphates activity, and calcium content assays confirmed that the osteogenesis rate of hiPSCs cultured on PVDF‐PVA‐Go is significantly higher than other scaffolds. Here, we showed that the biocompatible, nontoxic, flexible, piezoelectric, highly porous and interconnected three‐dimensional structure of electrospun PVDF‐PVA‐Go scaffold in combination with hiPSCs (as the stem cells with significant advantageous in comparison to other types) makes them a highly promising scaffold‐stem cell system for bone remodeling medicine. There was no evidence for the superiority of PVDF‐GO or PVDF‐PVA scaffold for osteogenesis, compared to each other; however both of them showed better potentials as to PVDF scaffold. 1. In this study, electrospun scaffolds of polyvinylidene fluoride (PVDF), PVDF‐graphene oxide (GO), PVDF‐polyvinyl alcohol (PVA) and PVDF‐PVA‐GO were fabricated to study the osteogenic differentiation potential of induced pluripotent stem cells (iPSCs) while cultured on fabricated scaffolds. 2. Scanning electron microscopy, viability assay, relative gene expression analysis, immunocytochemistry, alkaline phosphatase activity and calcium content assays confirmed that the osteogenesis rate of human induced pluripotent stem cells (hiPSCs) cultured on PVDF‐PVA‐Go is significantly higher than other substrates. 3. Here, we showed that the biocompatible, nontoxic, flexible, piezoelectric, highly porous and interconnected three‐dimensional structure of electrospun PVDF‐PVA‐Go scaffold in combination with hiPSCs makes them a highly promising scaffold‐stem cell system for bone remodeling medicine.