Multiplex genome editing using a dCas9-cytidine deaminase fusion in Streptomyces

CRISPR/Cas-mediated genome editing has greatly facilitated the study of gene function in Streptomyces . However, it could not be efficiently employed in streptomycetes with low homologous recombination (HR) ability. Here, a deaminase-assisted base editor dCas9-CDA-UL str was developed in Streptomyce...

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Veröffentlicht in:	Science China. Life sciences 2020-07, Vol.63 (7), p.1053-1062
Hauptverfasser:	Zhao, Yawei, Tian, Jinzhong, Zheng, Guosong, Chen, Jun, Sun, Chuanwen, Yang, Zhongyi, Zimin, Andrei A., Jiang, Weihong, Deng, Zixin, Wang, Zhijun, Lu, Yinhua
Format:	Artikel
Sprache:	eng
Schlagworte:	Biomedical and Life Sciences CRISPR CRISPR-Associated Protein 9 - genetics CRISPR-Cas Systems - genetics Cytidine deaminase Cytidine Deaminase - genetics Cytosine Deoxyribonucleic acid DNA DNA glycosylase DNA repair Editors Gene Editing - methods Gene Expression Regulation, Bacterial Genetic Engineering Genome editing Genome, Bacterial - genetics Genomes Guanosine - metabolism Homologous recombination Immunosuppressive Agents - metabolism Life Sciences Metabolic engineering Nuclease Point Mutation - genetics Promoter Regions, Genetic Rapamycin Recombinant Proteins - genetics Research Paper Sirolimus - metabolism Streptomyces Streptomyces - genetics Thymine Uracil Uracil-DNA glycosidase
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