Novel small IncX3 plasmid carrying the blaKPC-2 gene in high-risk Klebsiella pneumoniae ST11/CG258
This study used whole-genome sequencing (WGS) and PFGE to analysis KPC-2-producing Klebsiella pneumoniae strains from clinical specimens collected in Brazilian hospitals. The study identifies the emergence of a novel small IncX3 plasmid (pKPB11), 12,757-bp in length, in a high-risk K. pneumoniae ST1...
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| Veröffentlicht in: | Diagnostic microbiology and infectious disease 2020-02, Vol.96 (2), p.114900-114900, Article 114900 |
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| container_title | Diagnostic microbiology and infectious disease |
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| creator | Fuga, Bruna Ferreira, Melina Lorraine Cerdeira, Louise Teixeira de Campos, Paola Amaral Dias, Vinícius Lopes Rossi, Iara Machado, Luiz Gustavo Lincopan, Nilton Gontijo-Filho, Paulo Pinto Ribas, Rosineide Marques |
| description | This study used whole-genome sequencing (WGS) and PFGE to analysis KPC-2-producing Klebsiella pneumoniae strains from clinical specimens collected in Brazilian hospitals. The study identifies the emergence of a novel small IncX3 plasmid (pKPB11), 12,757-bp in length, in a high-risk K. pneumoniae ST11/CG258 lineage, a successful clonal group in Brazil, carrying the blaKPC-2 gene on a non-Tn4401 genetic element (NTEKPC-Ic). Comparative analysis of the pKPB11 showed that this plasmid reduced its size, losing part of its conjugation apparatus. The pKPB11 was also compared to another strain sequenced in this study (KPC89) that had the hybrid IncX3-IncU plasmid (pKP89), of approximately 45 kb in length, similarly carrying the blaKPC-2 gene on NTEKPC-Ic. To the best of our knowledge, pKPB11 is the first example of small IncX3 plasmid found in a high-risk KPC-2-producing K. pneumoniae ST11/CG258. |
| doi_str_mv | 10.1016/j.diagmicrobio.2019.114900 |
| format | Article |
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| source | Elsevier ScienceDirect Journals |
| subjects | CG258 IncX3 Klebsiella pneumoniae KPC-2 NTEKPC |
| title | Novel small IncX3 plasmid carrying the blaKPC-2 gene in high-risk Klebsiella pneumoniae ST11/CG258 |
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