Biosolids recycling impact on biofilm extracellular enzyme activity and performance of hybrid rotating biological reactors
Biological processes for wastewater treatment is limited by extracellular enzyme activity (EEA) of the biofilm on polymeric substrates. The efficiency of biodegradation / biosorption mechanisms causing EEA and organic load removal in biofilms remains unknown. Our hypothesis was that the limiting ste...
Gespeichert in:
| Veröffentlicht in: | The Science of the total environment 2020-03, Vol.706, p.135865-135865, Article 135865 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 135865 |
|---|---|
| container_issue | |
| container_start_page | 135865 |
| container_title | The Science of the total environment |
| container_volume | 706 |
| creator | Hassard, Francis Biddle, Jeremy Cartmell, Elise Coulon, Frédéric Stephenson, Tom |
| description | Biological processes for wastewater treatment is limited by extracellular enzyme activity (EEA) of the biofilm on polymeric substrates. The efficiency of biodegradation / biosorption mechanisms causing EEA and organic load removal in biofilms remains unknown. Our hypothesis was that the limiting step of biological process can be overcome by biostimulation and/or bioaugmentation of the return sludge in hybrid biofilm reactors, which leads to competition between suspended and attached bacteria and lower effective substrate to microrganism ratio. Therefore, we considered more active biosolids to perform best at enhancing reactor removal rate. To test this, the efficacy of recycling distinct bio-solids types considered to have different bacterial activity such as final effluent (FE), humus solids (HS) and recycle activated sludge (RAS) on performance improvements of rotating biofilm reactors (RBRs). These bio-solids were investigated under high organic loading rates (OLR) and solids loading rates (SLR) using pilot scale reactors receiving real municipal wastewaters. Controlled overloading of RBRs revealed that EEA improved with increasing OLR/SLR. High SLR (>3.3 kg Total Suspended Solids m−2 d−1) delayed and decreased the reduction of organic and inorganic removal rates in the biological processes which commonly occurs under high OLRs. This effect was more pronounced in the highest activity solids (RAS > HS > FE) suggesting the activity and function of bio-solids was critical to improve performance of RBRs. High OLR and SLR induced efficient denitrification and organics removal within the biofilm reactor at residence times of |
| doi_str_mv | 10.1016/j.scitotenv.2019.135865 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2328350769</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0048969719358607</els_id><sourcerecordid>2328350769</sourcerecordid><originalsourceid>FETCH-LOGICAL-c420t-1b51641a8fb1c12b6e828444eb281c4c007d0d9e595b34a616a7bfc75f0aa05a3</originalsourceid><addsrcrecordid>eNqFkMtu1DAUhi0EokPhFcBLNhl8EttxlqXiJlViA2vLdk6KR0482J4R6dPjaEq3nM3Z_Bf9HyHvgO2Bgfxw2GfnSyy4nPctg2EPnVBSPCM7UP3QAGvlc7JjjKtmkEN_RV7lfGD1egUvyVUHikvVix15-OhjjsGPmSZ0qwt-uad-PhpXaFyo9XHyYab4pyTjMIRTMIni8rDOSKvGn31ZqVlGesQ0xTSbxSGNE_212uRHmmIxZYusQSHee2dC7anGmPJr8mIyIeObx39Nfn7-9OP2a3P3_cu325u7xvGWlQasAMnBqMmCg9ZKVK3inKNtFTju6qaRjQOKQdiOGwnS9HZyvZiYMUyY7pq8v-QeU_x9wlz07PO2xSwYT1m3Xas6wXo5VGl_kboUc0446WPys0mrBqY38Pqgn8DrDby-gK_Ot48lJzvj-OT7R7oKbi4CrFPPHtMWhJXW6Cv4osfo_1vyF__InEU</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2328350769</pqid></control><display><type>article</type><title>Biosolids recycling impact on biofilm extracellular enzyme activity and performance of hybrid rotating biological reactors</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Hassard, Francis ; Biddle, Jeremy ; Cartmell, Elise ; Coulon, Frédéric ; Stephenson, Tom</creator><creatorcontrib>Hassard, Francis ; Biddle, Jeremy ; Cartmell, Elise ; Coulon, Frédéric ; Stephenson, Tom</creatorcontrib><description>Biological processes for wastewater treatment is limited by extracellular enzyme activity (EEA) of the biofilm on polymeric substrates. The efficiency of biodegradation / biosorption mechanisms causing EEA and organic load removal in biofilms remains unknown. Our hypothesis was that the limiting step of biological process can be overcome by biostimulation and/or bioaugmentation of the return sludge in hybrid biofilm reactors, which leads to competition between suspended and attached bacteria and lower effective substrate to microrganism ratio. Therefore, we considered more active biosolids to perform best at enhancing reactor removal rate. To test this, the efficacy of recycling distinct bio-solids types considered to have different bacterial activity such as final effluent (FE), humus solids (HS) and recycle activated sludge (RAS) on performance improvements of rotating biofilm reactors (RBRs). These bio-solids were investigated under high organic loading rates (OLR) and solids loading rates (SLR) using pilot scale reactors receiving real municipal wastewaters. Controlled overloading of RBRs revealed that EEA improved with increasing OLR/SLR. High SLR (>3.3 kg Total Suspended Solids m−2 d−1) delayed and decreased the reduction of organic and inorganic removal rates in the biological processes which commonly occurs under high OLRs. This effect was more pronounced in the highest activity solids (RAS > HS > FE) suggesting the activity and function of bio-solids was critical to improve performance of RBRs. High OLR and SLR induced efficient denitrification and organics removal within the biofilm reactor at residence times of <5 min. Recycling active solids permitted EEA despite overloading which was critical to the performance of the RBRs.
[Display omitted]
•Solids augmentation improved maximum bulks organics and NH4-N removal ~six fold.•Final effluent feed had the maximum nitrogen removal rate 71 g·NOx-N·m−2d−1.•Amino-peptidase extracellular enzyme activity increased with organic load to 122 μM·gVS·min−1.•Organic load, solids load and type were important operating parameters governing EEA and performance.•Recycling active solids permitted high EEA despite overloading which improved performance.</description><identifier>ISSN: 0048-9697</identifier><identifier>EISSN: 1879-1026</identifier><identifier>DOI: 10.1016/j.scitotenv.2019.135865</identifier><identifier>PMID: 31846875</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Biofilm ; Biofilms ; Bioreactors ; Biosolids ; Denitrification ; Hybrid biological process ; Microbial extracellular enzyme activity ; Nitrogen ; Rotating biofilm contactor ; Sewage ; Waste Disposal, Fluid</subject><ispartof>The Science of the total environment, 2020-03, Vol.706, p.135865-135865, Article 135865</ispartof><rights>2019 The Authors</rights><rights>Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c420t-1b51641a8fb1c12b6e828444eb281c4c007d0d9e595b34a616a7bfc75f0aa05a3</citedby><cites>FETCH-LOGICAL-c420t-1b51641a8fb1c12b6e828444eb281c4c007d0d9e595b34a616a7bfc75f0aa05a3</cites><orcidid>0000-0003-4803-6523</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0048969719358607$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31846875$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hassard, Francis</creatorcontrib><creatorcontrib>Biddle, Jeremy</creatorcontrib><creatorcontrib>Cartmell, Elise</creatorcontrib><creatorcontrib>Coulon, Frédéric</creatorcontrib><creatorcontrib>Stephenson, Tom</creatorcontrib><title>Biosolids recycling impact on biofilm extracellular enzyme activity and performance of hybrid rotating biological reactors</title><title>The Science of the total environment</title><addtitle>Sci Total Environ</addtitle><description>Biological processes for wastewater treatment is limited by extracellular enzyme activity (EEA) of the biofilm on polymeric substrates. The efficiency of biodegradation / biosorption mechanisms causing EEA and organic load removal in biofilms remains unknown. Our hypothesis was that the limiting step of biological process can be overcome by biostimulation and/or bioaugmentation of the return sludge in hybrid biofilm reactors, which leads to competition between suspended and attached bacteria and lower effective substrate to microrganism ratio. Therefore, we considered more active biosolids to perform best at enhancing reactor removal rate. To test this, the efficacy of recycling distinct bio-solids types considered to have different bacterial activity such as final effluent (FE), humus solids (HS) and recycle activated sludge (RAS) on performance improvements of rotating biofilm reactors (RBRs). These bio-solids were investigated under high organic loading rates (OLR) and solids loading rates (SLR) using pilot scale reactors receiving real municipal wastewaters. Controlled overloading of RBRs revealed that EEA improved with increasing OLR/SLR. High SLR (>3.3 kg Total Suspended Solids m−2 d−1) delayed and decreased the reduction of organic and inorganic removal rates in the biological processes which commonly occurs under high OLRs. This effect was more pronounced in the highest activity solids (RAS > HS > FE) suggesting the activity and function of bio-solids was critical to improve performance of RBRs. High OLR and SLR induced efficient denitrification and organics removal within the biofilm reactor at residence times of <5 min. Recycling active solids permitted EEA despite overloading which was critical to the performance of the RBRs.
[Display omitted]
•Solids augmentation improved maximum bulks organics and NH4-N removal ~six fold.•Final effluent feed had the maximum nitrogen removal rate 71 g·NOx-N·m−2d−1.•Amino-peptidase extracellular enzyme activity increased with organic load to 122 μM·gVS·min−1.•Organic load, solids load and type were important operating parameters governing EEA and performance.•Recycling active solids permitted high EEA despite overloading which improved performance.</description><subject>Biofilm</subject><subject>Biofilms</subject><subject>Bioreactors</subject><subject>Biosolids</subject><subject>Denitrification</subject><subject>Hybrid biological process</subject><subject>Microbial extracellular enzyme activity</subject><subject>Nitrogen</subject><subject>Rotating biofilm contactor</subject><subject>Sewage</subject><subject>Waste Disposal, Fluid</subject><issn>0048-9697</issn><issn>1879-1026</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtu1DAUhi0EokPhFcBLNhl8EttxlqXiJlViA2vLdk6KR0482J4R6dPjaEq3nM3Z_Bf9HyHvgO2Bgfxw2GfnSyy4nPctg2EPnVBSPCM7UP3QAGvlc7JjjKtmkEN_RV7lfGD1egUvyVUHikvVix15-OhjjsGPmSZ0qwt-uad-PhpXaFyo9XHyYab4pyTjMIRTMIni8rDOSKvGn31ZqVlGesQ0xTSbxSGNE_212uRHmmIxZYusQSHee2dC7anGmPJr8mIyIeObx39Nfn7-9OP2a3P3_cu325u7xvGWlQasAMnBqMmCg9ZKVK3inKNtFTju6qaRjQOKQdiOGwnS9HZyvZiYMUyY7pq8v-QeU_x9wlz07PO2xSwYT1m3Xas6wXo5VGl_kboUc0446WPys0mrBqY38Pqgn8DrDby-gK_Ot48lJzvj-OT7R7oKbi4CrFPPHtMWhJXW6Cv4osfo_1vyF__InEU</recordid><startdate>20200301</startdate><enddate>20200301</enddate><creator>Hassard, Francis</creator><creator>Biddle, Jeremy</creator><creator>Cartmell, Elise</creator><creator>Coulon, Frédéric</creator><creator>Stephenson, Tom</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-4803-6523</orcidid></search><sort><creationdate>20200301</creationdate><title>Biosolids recycling impact on biofilm extracellular enzyme activity and performance of hybrid rotating biological reactors</title><author>Hassard, Francis ; Biddle, Jeremy ; Cartmell, Elise ; Coulon, Frédéric ; Stephenson, Tom</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c420t-1b51641a8fb1c12b6e828444eb281c4c007d0d9e595b34a616a7bfc75f0aa05a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Biofilm</topic><topic>Biofilms</topic><topic>Bioreactors</topic><topic>Biosolids</topic><topic>Denitrification</topic><topic>Hybrid biological process</topic><topic>Microbial extracellular enzyme activity</topic><topic>Nitrogen</topic><topic>Rotating biofilm contactor</topic><topic>Sewage</topic><topic>Waste Disposal, Fluid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hassard, Francis</creatorcontrib><creatorcontrib>Biddle, Jeremy</creatorcontrib><creatorcontrib>Cartmell, Elise</creatorcontrib><creatorcontrib>Coulon, Frédéric</creatorcontrib><creatorcontrib>Stephenson, Tom</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Science of the total environment</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hassard, Francis</au><au>Biddle, Jeremy</au><au>Cartmell, Elise</au><au>Coulon, Frédéric</au><au>Stephenson, Tom</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biosolids recycling impact on biofilm extracellular enzyme activity and performance of hybrid rotating biological reactors</atitle><jtitle>The Science of the total environment</jtitle><addtitle>Sci Total Environ</addtitle><date>2020-03-01</date><risdate>2020</risdate><volume>706</volume><spage>135865</spage><epage>135865</epage><pages>135865-135865</pages><artnum>135865</artnum><issn>0048-9697</issn><eissn>1879-1026</eissn><abstract>Biological processes for wastewater treatment is limited by extracellular enzyme activity (EEA) of the biofilm on polymeric substrates. The efficiency of biodegradation / biosorption mechanisms causing EEA and organic load removal in biofilms remains unknown. Our hypothesis was that the limiting step of biological process can be overcome by biostimulation and/or bioaugmentation of the return sludge in hybrid biofilm reactors, which leads to competition between suspended and attached bacteria and lower effective substrate to microrganism ratio. Therefore, we considered more active biosolids to perform best at enhancing reactor removal rate. To test this, the efficacy of recycling distinct bio-solids types considered to have different bacterial activity such as final effluent (FE), humus solids (HS) and recycle activated sludge (RAS) on performance improvements of rotating biofilm reactors (RBRs). These bio-solids were investigated under high organic loading rates (OLR) and solids loading rates (SLR) using pilot scale reactors receiving real municipal wastewaters. Controlled overloading of RBRs revealed that EEA improved with increasing OLR/SLR. High SLR (>3.3 kg Total Suspended Solids m−2 d−1) delayed and decreased the reduction of organic and inorganic removal rates in the biological processes which commonly occurs under high OLRs. This effect was more pronounced in the highest activity solids (RAS > HS > FE) suggesting the activity and function of bio-solids was critical to improve performance of RBRs. High OLR and SLR induced efficient denitrification and organics removal within the biofilm reactor at residence times of <5 min. Recycling active solids permitted EEA despite overloading which was critical to the performance of the RBRs.
[Display omitted]
•Solids augmentation improved maximum bulks organics and NH4-N removal ~six fold.•Final effluent feed had the maximum nitrogen removal rate 71 g·NOx-N·m−2d−1.•Amino-peptidase extracellular enzyme activity increased with organic load to 122 μM·gVS·min−1.•Organic load, solids load and type were important operating parameters governing EEA and performance.•Recycling active solids permitted high EEA despite overloading which improved performance.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>31846875</pmid><doi>10.1016/j.scitotenv.2019.135865</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0003-4803-6523</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0048-9697 |
| ispartof | The Science of the total environment, 2020-03, Vol.706, p.135865-135865, Article 135865 |
| issn | 0048-9697 1879-1026 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2328350769 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Biofilm Biofilms Bioreactors Biosolids Denitrification Hybrid biological process Microbial extracellular enzyme activity Nitrogen Rotating biofilm contactor Sewage Waste Disposal, Fluid |
| title | Biosolids recycling impact on biofilm extracellular enzyme activity and performance of hybrid rotating biological reactors |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-03T06%3A43%3A07IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Biosolids%20recycling%20impact%20on%20biofilm%20extracellular%20enzyme%20activity%20and%20performance%20of%20hybrid%20rotating%20biological%20reactors&rft.jtitle=The%20Science%20of%20the%20total%20environment&rft.au=Hassard,%20Francis&rft.date=2020-03-01&rft.volume=706&rft.spage=135865&rft.epage=135865&rft.pages=135865-135865&rft.artnum=135865&rft.issn=0048-9697&rft.eissn=1879-1026&rft_id=info:doi/10.1016/j.scitotenv.2019.135865&rft_dat=%3Cproquest_cross%3E2328350769%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2328350769&rft_id=info:pmid/31846875&rft_els_id=S0048969719358607&rfr_iscdi=true |