Novel polymeric microspheres: Synthesis, enzyme immobilization, antimutagenic activity, and antimicrobial evaluation against pathogenic microorganisms
New polymeric microspheres containing azomethine (1a‐1c and 2a‐2c) were synthesized by condensation to compare the enzymatic properties of the enzyme glucose oxidase (GOx) and to investigate antimutagenic and antimicrobial activities. The polymeric microspheres were characterized by elemental analys...
Gespeichert in:
| Veröffentlicht in: | Journal of biochemical and molecular toxicology 2020-02, Vol.34 (2), p.e22432-n/a |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | n/a |
|---|---|
| container_issue | 2 |
| container_start_page | e22432 |
| container_title | Journal of biochemical and molecular toxicology |
| container_volume | 34 |
| creator | Nartop, Dilek Demirel, Birtane Güleç, Murat Hasanoğlu Özkan, Elvan Kurnaz Yetim, Nurdan Sarı, Nurşen Çeker, Selçuk Öğütcü, Hatice Ağar, Güleray |
| description | New polymeric microspheres containing azomethine (1a‐1c and 2a‐2c) were synthesized by condensation to compare the enzymatic properties of the enzyme glucose oxidase (GOx) and to investigate antimutagenic and antimicrobial activities. The polymeric microspheres were characterized by elemental analysis, infrared spectra (FT‐IR), proton nuclear magnetic resonance spectra, thermal gravimetric analysis, and scanning electron microscopy analysis. The catalytic activity of the glucose oxidase enzyme follows Michaelis‐Menten kinetics. Influence of temperature, reusability, and storage capacity of the free and immobilized glucose oxidase enzyme were investigated. It is determined that immobilized enzymes exhibit good storage stability and reusability. After immobilization of GOx in polymeric supports, the thermal stability of the enzyme increased and the maximum reaction rate (Vmax) decreased. The activity of the immobilized enzymes was preserved even after 5 months. The antibacterial and antifungal activity of the polymeric microspheres were evaluated by well‐diffusion method against some selected pathogenic microorganisms. The antimutagenic properties of all compounds were also examined against sodium azide in human lymphocyte cells by micronuclei and sister chromatid exchange tests.
Highlights
Novel polymeric microspheres including azomethine with Pt(IV) were synthesized by means of condensation method.
Polymeric microspheres were characterized by means of spectral measurements.
Glucose oxidase (GOx) enzyme was covalently immobilized on these polymeric microspheres and investigated the enzymatic properties of GOx.
The catalytic activity of GOx enzyme followed Michaelis‐Menten kinetics.
The antibacterial and antifungal activities of all polymeric microspheres were investigated by the well‐diffusion method as antimicrobial agents.
The antimutagenic properties of these polymeric microspheres were evaluated against sodium azide (NaN3) in human lymphocyte cells by micronuclei (MN) and sister chromatid exchange (SCE) tests. |
| doi_str_mv | 10.1002/jbt.22432 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2328349281</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2351936166</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3532-c394cb2356ad8383e6a8feb39091fa074ac3f1be78d21de033caba39de5bffae3</originalsourceid><addsrcrecordid>eNp1kctu1DAUhiMEoqWw4AWQJTYgdVpfkkzMDiquqmBBWUfHycmMR76ktjMofRCeF8-ksEBiY1v293868l8Uzxm9YJTyy51KF5yXgj8oThmVckXLmj08nqtVXa_pSfEkxh2ltJLr6nFxIlhTsZKK0-LXV79HQ0ZvZotBd8TqLvg4bjFgfEO-zy5tMep4TtDdZYRoa73SRt9B0t6dE3BJ2ynBBl1OQ5f0Xqf5cN8vbwef0mAI7sFMxxSBDWgXExkhbf2SPHI-bMDpaOPT4tEAJuKz-_2s-PHh_c3Vp9X1t4-fr95erzpRCZ5XWXaKi6qGvhGNwBqaAZWQVLIB6LqETgxM4brpOeuRCtGBAiF7rNQwAIqz4tXiHYO_nTCm1urYoTHg0E-x5YI3opS8YRl9-Q-681NwebpMVUyKmtV1pl4v1OEXY8ChHYO2EOaW0fZQVpvLao9lZfbFvXFSFvu_5J92MnC5AD-1wfn_pvbLu5tF-RsZSKMl</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2351936166</pqid></control><display><type>article</type><title>Novel polymeric microspheres: Synthesis, enzyme immobilization, antimutagenic activity, and antimicrobial evaluation against pathogenic microorganisms</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Nartop, Dilek ; Demirel, Birtane ; Güleç, Murat ; Hasanoğlu Özkan, Elvan ; Kurnaz Yetim, Nurdan ; Sarı, Nurşen ; Çeker, Selçuk ; Öğütcü, Hatice ; Ağar, Güleray</creator><creatorcontrib>Nartop, Dilek ; Demirel, Birtane ; Güleç, Murat ; Hasanoğlu Özkan, Elvan ; Kurnaz Yetim, Nurdan ; Sarı, Nurşen ; Çeker, Selçuk ; Öğütcü, Hatice ; Ağar, Güleray</creatorcontrib><description>New polymeric microspheres containing azomethine (1a‐1c and 2a‐2c) were synthesized by condensation to compare the enzymatic properties of the enzyme glucose oxidase (GOx) and to investigate antimutagenic and antimicrobial activities. The polymeric microspheres were characterized by elemental analysis, infrared spectra (FT‐IR), proton nuclear magnetic resonance spectra, thermal gravimetric analysis, and scanning electron microscopy analysis. The catalytic activity of the glucose oxidase enzyme follows Michaelis‐Menten kinetics. Influence of temperature, reusability, and storage capacity of the free and immobilized glucose oxidase enzyme were investigated. It is determined that immobilized enzymes exhibit good storage stability and reusability. After immobilization of GOx in polymeric supports, the thermal stability of the enzyme increased and the maximum reaction rate (Vmax) decreased. The activity of the immobilized enzymes was preserved even after 5 months. The antibacterial and antifungal activity of the polymeric microspheres were evaluated by well‐diffusion method against some selected pathogenic microorganisms. The antimutagenic properties of all compounds were also examined against sodium azide in human lymphocyte cells by micronuclei and sister chromatid exchange tests.
Highlights
Novel polymeric microspheres including azomethine with Pt(IV) were synthesized by means of condensation method.
Polymeric microspheres were characterized by means of spectral measurements.
Glucose oxidase (GOx) enzyme was covalently immobilized on these polymeric microspheres and investigated the enzymatic properties of GOx.
The catalytic activity of GOx enzyme followed Michaelis‐Menten kinetics.
The antibacterial and antifungal activities of all polymeric microspheres were investigated by the well‐diffusion method as antimicrobial agents.
The antimutagenic properties of these polymeric microspheres were evaluated against sodium azide (NaN3) in human lymphocyte cells by micronuclei (MN) and sister chromatid exchange (SCE) tests.</description><identifier>ISSN: 1095-6670</identifier><identifier>EISSN: 1099-0461</identifier><identifier>DOI: 10.1002/jbt.22432</identifier><identifier>PMID: 31851403</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>Anti-Infective Agents - pharmacology ; Antifungal activity ; Antimicrobial agents ; antimicrobial property ; Antimutagenic Agents - pharmacology ; antimutagenic effect ; Azo Compounds - chemistry ; Candida albicans - drug effects ; Catalytic activity ; Cells, Cultured ; Chemical synthesis ; Condensation polymerization ; Enzymes ; Enzymes, Immobilized - chemistry ; Enzymes, Immobilized - pharmacokinetics ; Evaluation ; Exchanging ; Female ; Fungicides ; Glucose ; Glucose oxidase ; Glucose Oxidase - chemistry ; Glucose Oxidase - pharmacokinetics ; Gram-Negative Bacteria - drug effects ; Gram-Positive Bacteria - drug effects ; Gravimetric analysis ; Healthy Volunteers ; Humans ; Hydrogen-Ion Concentration ; Immobilization ; Immobilized enzymes ; Infrared analysis ; Infrared spectra ; Kinetics ; Lymphocytes ; Lymphocytes - drug effects ; Lymphocytes - metabolism ; Male ; Micronuclei ; Micronucleus Tests ; Microorganisms ; Microscopy, Electron, Scanning ; Microspheres ; NMR ; Nuclear magnetic resonance ; Polymeric microsphere ; Properties (attributes) ; Pt4+‐azomethine ; Reaction kinetics ; Scanning electron microscopy ; Shelf life ; Sister chromatid exchange ; Sister Chromatid Exchange - drug effects ; Sodium ; Sodium azide ; Sodium Azide - adverse effects ; Sodium Azide - pharmacology ; Sodium azides ; Storage capacity ; Storage stability ; Temperature ; Thermal stability ; Thiosemicarbazones - chemistry</subject><ispartof>Journal of biochemical and molecular toxicology, 2020-02, Vol.34 (2), p.e22432-n/a</ispartof><rights>2019 Wiley Periodicals, Inc.</rights><rights>2020 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3532-c394cb2356ad8383e6a8feb39091fa074ac3f1be78d21de033caba39de5bffae3</citedby><cites>FETCH-LOGICAL-c3532-c394cb2356ad8383e6a8feb39091fa074ac3f1be78d21de033caba39de5bffae3</cites><orcidid>0000-0002-0705-5018 ; 0000-0001-6227-0346</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjbt.22432$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjbt.22432$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31851403$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nartop, Dilek</creatorcontrib><creatorcontrib>Demirel, Birtane</creatorcontrib><creatorcontrib>Güleç, Murat</creatorcontrib><creatorcontrib>Hasanoğlu Özkan, Elvan</creatorcontrib><creatorcontrib>Kurnaz Yetim, Nurdan</creatorcontrib><creatorcontrib>Sarı, Nurşen</creatorcontrib><creatorcontrib>Çeker, Selçuk</creatorcontrib><creatorcontrib>Öğütcü, Hatice</creatorcontrib><creatorcontrib>Ağar, Güleray</creatorcontrib><title>Novel polymeric microspheres: Synthesis, enzyme immobilization, antimutagenic activity, and antimicrobial evaluation against pathogenic microorganisms</title><title>Journal of biochemical and molecular toxicology</title><addtitle>J Biochem Mol Toxicol</addtitle><description>New polymeric microspheres containing azomethine (1a‐1c and 2a‐2c) were synthesized by condensation to compare the enzymatic properties of the enzyme glucose oxidase (GOx) and to investigate antimutagenic and antimicrobial activities. The polymeric microspheres were characterized by elemental analysis, infrared spectra (FT‐IR), proton nuclear magnetic resonance spectra, thermal gravimetric analysis, and scanning electron microscopy analysis. The catalytic activity of the glucose oxidase enzyme follows Michaelis‐Menten kinetics. Influence of temperature, reusability, and storage capacity of the free and immobilized glucose oxidase enzyme were investigated. It is determined that immobilized enzymes exhibit good storage stability and reusability. After immobilization of GOx in polymeric supports, the thermal stability of the enzyme increased and the maximum reaction rate (Vmax) decreased. The activity of the immobilized enzymes was preserved even after 5 months. The antibacterial and antifungal activity of the polymeric microspheres were evaluated by well‐diffusion method against some selected pathogenic microorganisms. The antimutagenic properties of all compounds were also examined against sodium azide in human lymphocyte cells by micronuclei and sister chromatid exchange tests.
Highlights
Novel polymeric microspheres including azomethine with Pt(IV) were synthesized by means of condensation method.
Polymeric microspheres were characterized by means of spectral measurements.
Glucose oxidase (GOx) enzyme was covalently immobilized on these polymeric microspheres and investigated the enzymatic properties of GOx.
The catalytic activity of GOx enzyme followed Michaelis‐Menten kinetics.
The antibacterial and antifungal activities of all polymeric microspheres were investigated by the well‐diffusion method as antimicrobial agents.
The antimutagenic properties of these polymeric microspheres were evaluated against sodium azide (NaN3) in human lymphocyte cells by micronuclei (MN) and sister chromatid exchange (SCE) tests.</description><subject>Anti-Infective Agents - pharmacology</subject><subject>Antifungal activity</subject><subject>Antimicrobial agents</subject><subject>antimicrobial property</subject><subject>Antimutagenic Agents - pharmacology</subject><subject>antimutagenic effect</subject><subject>Azo Compounds - chemistry</subject><subject>Candida albicans - drug effects</subject><subject>Catalytic activity</subject><subject>Cells, Cultured</subject><subject>Chemical synthesis</subject><subject>Condensation polymerization</subject><subject>Enzymes</subject><subject>Enzymes, Immobilized - chemistry</subject><subject>Enzymes, Immobilized - pharmacokinetics</subject><subject>Evaluation</subject><subject>Exchanging</subject><subject>Female</subject><subject>Fungicides</subject><subject>Glucose</subject><subject>Glucose oxidase</subject><subject>Glucose Oxidase - chemistry</subject><subject>Glucose Oxidase - pharmacokinetics</subject><subject>Gram-Negative Bacteria - drug effects</subject><subject>Gram-Positive Bacteria - drug effects</subject><subject>Gravimetric analysis</subject><subject>Healthy Volunteers</subject><subject>Humans</subject><subject>Hydrogen-Ion Concentration</subject><subject>Immobilization</subject><subject>Immobilized enzymes</subject><subject>Infrared analysis</subject><subject>Infrared spectra</subject><subject>Kinetics</subject><subject>Lymphocytes</subject><subject>Lymphocytes - drug effects</subject><subject>Lymphocytes - metabolism</subject><subject>Male</subject><subject>Micronuclei</subject><subject>Micronucleus Tests</subject><subject>Microorganisms</subject><subject>Microscopy, Electron, Scanning</subject><subject>Microspheres</subject><subject>NMR</subject><subject>Nuclear magnetic resonance</subject><subject>Polymeric microsphere</subject><subject>Properties (attributes)</subject><subject>Pt4+‐azomethine</subject><subject>Reaction kinetics</subject><subject>Scanning electron microscopy</subject><subject>Shelf life</subject><subject>Sister chromatid exchange</subject><subject>Sister Chromatid Exchange - drug effects</subject><subject>Sodium</subject><subject>Sodium azide</subject><subject>Sodium Azide - adverse effects</subject><subject>Sodium Azide - pharmacology</subject><subject>Sodium azides</subject><subject>Storage capacity</subject><subject>Storage stability</subject><subject>Temperature</subject><subject>Thermal stability</subject><subject>Thiosemicarbazones - chemistry</subject><issn>1095-6670</issn><issn>1099-0461</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kctu1DAUhiMEoqWw4AWQJTYgdVpfkkzMDiquqmBBWUfHycmMR76ktjMofRCeF8-ksEBiY1v293868l8Uzxm9YJTyy51KF5yXgj8oThmVckXLmj08nqtVXa_pSfEkxh2ltJLr6nFxIlhTsZKK0-LXV79HQ0ZvZotBd8TqLvg4bjFgfEO-zy5tMep4TtDdZYRoa73SRt9B0t6dE3BJ2ynBBl1OQ5f0Xqf5cN8vbwef0mAI7sFMxxSBDWgXExkhbf2SPHI-bMDpaOPT4tEAJuKz-_2s-PHh_c3Vp9X1t4-fr95erzpRCZ5XWXaKi6qGvhGNwBqaAZWQVLIB6LqETgxM4brpOeuRCtGBAiF7rNQwAIqz4tXiHYO_nTCm1urYoTHg0E-x5YI3opS8YRl9-Q-681NwebpMVUyKmtV1pl4v1OEXY8ChHYO2EOaW0fZQVpvLao9lZfbFvXFSFvu_5J92MnC5AD-1wfn_pvbLu5tF-RsZSKMl</recordid><startdate>202002</startdate><enddate>202002</enddate><creator>Nartop, Dilek</creator><creator>Demirel, Birtane</creator><creator>Güleç, Murat</creator><creator>Hasanoğlu Özkan, Elvan</creator><creator>Kurnaz Yetim, Nurdan</creator><creator>Sarı, Nurşen</creator><creator>Çeker, Selçuk</creator><creator>Öğütcü, Hatice</creator><creator>Ağar, Güleray</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-0705-5018</orcidid><orcidid>https://orcid.org/0000-0001-6227-0346</orcidid></search><sort><creationdate>202002</creationdate><title>Novel polymeric microspheres: Synthesis, enzyme immobilization, antimutagenic activity, and antimicrobial evaluation against pathogenic microorganisms</title><author>Nartop, Dilek ; Demirel, Birtane ; Güleç, Murat ; Hasanoğlu Özkan, Elvan ; Kurnaz Yetim, Nurdan ; Sarı, Nurşen ; Çeker, Selçuk ; Öğütcü, Hatice ; Ağar, Güleray</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3532-c394cb2356ad8383e6a8feb39091fa074ac3f1be78d21de033caba39de5bffae3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Anti-Infective Agents - pharmacology</topic><topic>Antifungal activity</topic><topic>Antimicrobial agents</topic><topic>antimicrobial property</topic><topic>Antimutagenic Agents - pharmacology</topic><topic>antimutagenic effect</topic><topic>Azo Compounds - chemistry</topic><topic>Candida albicans - drug effects</topic><topic>Catalytic activity</topic><topic>Cells, Cultured</topic><topic>Chemical synthesis</topic><topic>Condensation polymerization</topic><topic>Enzymes</topic><topic>Enzymes, Immobilized - chemistry</topic><topic>Enzymes, Immobilized - pharmacokinetics</topic><topic>Evaluation</topic><topic>Exchanging</topic><topic>Female</topic><topic>Fungicides</topic><topic>Glucose</topic><topic>Glucose oxidase</topic><topic>Glucose Oxidase - chemistry</topic><topic>Glucose Oxidase - pharmacokinetics</topic><topic>Gram-Negative Bacteria - drug effects</topic><topic>Gram-Positive Bacteria - drug effects</topic><topic>Gravimetric analysis</topic><topic>Healthy Volunteers</topic><topic>Humans</topic><topic>Hydrogen-Ion Concentration</topic><topic>Immobilization</topic><topic>Immobilized enzymes</topic><topic>Infrared analysis</topic><topic>Infrared spectra</topic><topic>Kinetics</topic><topic>Lymphocytes</topic><topic>Lymphocytes - drug effects</topic><topic>Lymphocytes - metabolism</topic><topic>Male</topic><topic>Micronuclei</topic><topic>Micronucleus Tests</topic><topic>Microorganisms</topic><topic>Microscopy, Electron, Scanning</topic><topic>Microspheres</topic><topic>NMR</topic><topic>Nuclear magnetic resonance</topic><topic>Polymeric microsphere</topic><topic>Properties (attributes)</topic><topic>Pt4+‐azomethine</topic><topic>Reaction kinetics</topic><topic>Scanning electron microscopy</topic><topic>Shelf life</topic><topic>Sister chromatid exchange</topic><topic>Sister Chromatid Exchange - drug effects</topic><topic>Sodium</topic><topic>Sodium azide</topic><topic>Sodium Azide - adverse effects</topic><topic>Sodium Azide - pharmacology</topic><topic>Sodium azides</topic><topic>Storage capacity</topic><topic>Storage stability</topic><topic>Temperature</topic><topic>Thermal stability</topic><topic>Thiosemicarbazones - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nartop, Dilek</creatorcontrib><creatorcontrib>Demirel, Birtane</creatorcontrib><creatorcontrib>Güleç, Murat</creatorcontrib><creatorcontrib>Hasanoğlu Özkan, Elvan</creatorcontrib><creatorcontrib>Kurnaz Yetim, Nurdan</creatorcontrib><creatorcontrib>Sarı, Nurşen</creatorcontrib><creatorcontrib>Çeker, Selçuk</creatorcontrib><creatorcontrib>Öğütcü, Hatice</creatorcontrib><creatorcontrib>Ağar, Güleray</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of biochemical and molecular toxicology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nartop, Dilek</au><au>Demirel, Birtane</au><au>Güleç, Murat</au><au>Hasanoğlu Özkan, Elvan</au><au>Kurnaz Yetim, Nurdan</au><au>Sarı, Nurşen</au><au>Çeker, Selçuk</au><au>Öğütcü, Hatice</au><au>Ağar, Güleray</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Novel polymeric microspheres: Synthesis, enzyme immobilization, antimutagenic activity, and antimicrobial evaluation against pathogenic microorganisms</atitle><jtitle>Journal of biochemical and molecular toxicology</jtitle><addtitle>J Biochem Mol Toxicol</addtitle><date>2020-02</date><risdate>2020</risdate><volume>34</volume><issue>2</issue><spage>e22432</spage><epage>n/a</epage><pages>e22432-n/a</pages><issn>1095-6670</issn><eissn>1099-0461</eissn><abstract>New polymeric microspheres containing azomethine (1a‐1c and 2a‐2c) were synthesized by condensation to compare the enzymatic properties of the enzyme glucose oxidase (GOx) and to investigate antimutagenic and antimicrobial activities. The polymeric microspheres were characterized by elemental analysis, infrared spectra (FT‐IR), proton nuclear magnetic resonance spectra, thermal gravimetric analysis, and scanning electron microscopy analysis. The catalytic activity of the glucose oxidase enzyme follows Michaelis‐Menten kinetics. Influence of temperature, reusability, and storage capacity of the free and immobilized glucose oxidase enzyme were investigated. It is determined that immobilized enzymes exhibit good storage stability and reusability. After immobilization of GOx in polymeric supports, the thermal stability of the enzyme increased and the maximum reaction rate (Vmax) decreased. The activity of the immobilized enzymes was preserved even after 5 months. The antibacterial and antifungal activity of the polymeric microspheres were evaluated by well‐diffusion method against some selected pathogenic microorganisms. The antimutagenic properties of all compounds were also examined against sodium azide in human lymphocyte cells by micronuclei and sister chromatid exchange tests.
Highlights
Novel polymeric microspheres including azomethine with Pt(IV) were synthesized by means of condensation method.
Polymeric microspheres were characterized by means of spectral measurements.
Glucose oxidase (GOx) enzyme was covalently immobilized on these polymeric microspheres and investigated the enzymatic properties of GOx.
The catalytic activity of GOx enzyme followed Michaelis‐Menten kinetics.
The antibacterial and antifungal activities of all polymeric microspheres were investigated by the well‐diffusion method as antimicrobial agents.
The antimutagenic properties of these polymeric microspheres were evaluated against sodium azide (NaN3) in human lymphocyte cells by micronuclei (MN) and sister chromatid exchange (SCE) tests.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>31851403</pmid><doi>10.1002/jbt.22432</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0002-0705-5018</orcidid><orcidid>https://orcid.org/0000-0001-6227-0346</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1095-6670 |
| ispartof | Journal of biochemical and molecular toxicology, 2020-02, Vol.34 (2), p.e22432-n/a |
| issn | 1095-6670 1099-0461 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2328349281 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Anti-Infective Agents - pharmacology Antifungal activity Antimicrobial agents antimicrobial property Antimutagenic Agents - pharmacology antimutagenic effect Azo Compounds - chemistry Candida albicans - drug effects Catalytic activity Cells, Cultured Chemical synthesis Condensation polymerization Enzymes Enzymes, Immobilized - chemistry Enzymes, Immobilized - pharmacokinetics Evaluation Exchanging Female Fungicides Glucose Glucose oxidase Glucose Oxidase - chemistry Glucose Oxidase - pharmacokinetics Gram-Negative Bacteria - drug effects Gram-Positive Bacteria - drug effects Gravimetric analysis Healthy Volunteers Humans Hydrogen-Ion Concentration Immobilization Immobilized enzymes Infrared analysis Infrared spectra Kinetics Lymphocytes Lymphocytes - drug effects Lymphocytes - metabolism Male Micronuclei Micronucleus Tests Microorganisms Microscopy, Electron, Scanning Microspheres NMR Nuclear magnetic resonance Polymeric microsphere Properties (attributes) Pt4+‐azomethine Reaction kinetics Scanning electron microscopy Shelf life Sister chromatid exchange Sister Chromatid Exchange - drug effects Sodium Sodium azide Sodium Azide - adverse effects Sodium Azide - pharmacology Sodium azides Storage capacity Storage stability Temperature Thermal stability Thiosemicarbazones - chemistry |
| title | Novel polymeric microspheres: Synthesis, enzyme immobilization, antimutagenic activity, and antimicrobial evaluation against pathogenic microorganisms |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-07T21%3A06%3A00IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Novel%20polymeric%20microspheres:%20Synthesis,%20enzyme%20immobilization,%20antimutagenic%20activity,%20and%20antimicrobial%20evaluation%20against%20pathogenic%20microorganisms&rft.jtitle=Journal%20of%20biochemical%20and%20molecular%20toxicology&rft.au=Nartop,%20Dilek&rft.date=2020-02&rft.volume=34&rft.issue=2&rft.spage=e22432&rft.epage=n/a&rft.pages=e22432-n/a&rft.issn=1095-6670&rft.eissn=1099-0461&rft_id=info:doi/10.1002/jbt.22432&rft_dat=%3Cproquest_cross%3E2351936166%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2351936166&rft_id=info:pmid/31851403&rfr_iscdi=true |