Effects of dietary arachidonic acid in European sea bass (Dicentrarchus labrax) distal intestine lipid classes and gut health
The use of low fishmeal/fish oil in marine fish diets affects dietary essential fatty acids (EFAs) composition and concentration and, subsequently, may produce a marginal deficiency of those fatty acids with a direct impact on the fish intestinal physiology. Supplementation of essential fatty acids...
Gespeichert in:
| Veröffentlicht in: | Fish physiology and biochemistry 2020-04, Vol.46 (2), p.681-697 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 697 |
|---|---|
| container_issue | 2 |
| container_start_page | 681 |
| container_title | Fish physiology and biochemistry |
| container_volume | 46 |
| creator | Rivero-Ramírez, F. Torrecillas, S. Betancor, M. B. Izquierdo, M. S. Caballero, M. J. Montero, D. |
| description | The use of low fishmeal/fish oil in marine fish diets affects dietary essential fatty acids (EFAs) composition and concentration and, subsequently, may produce a marginal deficiency of those fatty acids with a direct impact on the fish intestinal physiology. Supplementation of essential fatty acids is necessary to cover the requirements of the different EFAs, including the ones belonging to the n-6 series, such as arachidonic acid (ARA). ARA, besides its structural role in the configuration of the lipid classes of the intestine, plays an important role in the functionality of the gut-associated immune tissue (GALT). The present study aimed to test five levels of dietary ARA (ARA0.5 (0.5%), ARA1 (1%), ARA2 (2%), ARA4 (4%), and ARA6 (6%)) for European sea bass (
Dicentrarchus labrax
) juveniles in order to determine (a) its effect in selected distal intestine (DI) lipid classes composition and (b) how these changes affected gut bacterial translocation rates and selected GALT-related gene expression pre and post challenge. No differences were found between distal intestines of fish fed with the graded ARA levels in total neutral lipids and total polar lipids. However, DI of fish fed with the ARA6 diet presented a higher (
P
|
| doi_str_mv | 10.1007/s10695-019-00744-0 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2327941900</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2371196475</sourcerecordid><originalsourceid>FETCH-LOGICAL-c419t-e70d09dedaa599478f9c4bb063f545f48fb270606646ed613e9081b62221f5ce3</originalsourceid><addsrcrecordid>eNp9kU1vFSEUhomxsdfqH3BhSNzUxdQDw8ewNPX6kTTppq4Jwxx6aeYyV2ASu_C_S71VExeuCOF5n3PCS8grBhcMQL8rDJSRHTDTtasQHTwhGyZ130mmhqdkA4ZDx7Tgp-R5KXcAYLRiz8hpzwYhQZsN-bENAX0tdAl0ilhdvqcuO7-L05Kip87HicZEt2teDugSLejo6Eqh5x-ix1Szy363Fjq7Mbvvb5ukVDe3SMVSY0I6x0NT-LllsFCXJnq7VrpDN9fdC3IS3Fzw5eN5Rr5-3N5cfu6urj99uXx_1XnBTO1QwwRmwsk5aYzQQzBejCOoPkghgxjCyDUoUEoonBTr0cDARsU5Z0F67M_I-dF7yMu3tS1m97F4nGeXcFmL5T3Xpo0CaOibf9C7Zc2pbdcozZhRQstG8SPl81JKxmAPOe7b51kG9qEceyzHtnLsr3Lsg_r1o3od9zj9ifxuowH9ESjtKd1i_jv7P9qffhaaIg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2371196475</pqid></control><display><type>article</type><title>Effects of dietary arachidonic acid in European sea bass (Dicentrarchus labrax) distal intestine lipid classes and gut health</title><source>SpringerLink_现刊</source><creator>Rivero-Ramírez, F. ; Torrecillas, S. ; Betancor, M. B. ; Izquierdo, M. S. ; Caballero, M. J. ; Montero, D.</creator><creatorcontrib>Rivero-Ramírez, F. ; Torrecillas, S. ; Betancor, M. B. ; Izquierdo, M. S. ; Caballero, M. J. ; Montero, D.</creatorcontrib><description>The use of low fishmeal/fish oil in marine fish diets affects dietary essential fatty acids (EFAs) composition and concentration and, subsequently, may produce a marginal deficiency of those fatty acids with a direct impact on the fish intestinal physiology. Supplementation of essential fatty acids is necessary to cover the requirements of the different EFAs, including the ones belonging to the n-6 series, such as arachidonic acid (ARA). ARA, besides its structural role in the configuration of the lipid classes of the intestine, plays an important role in the functionality of the gut-associated immune tissue (GALT). The present study aimed to test five levels of dietary ARA (ARA0.5 (0.5%), ARA1 (1%), ARA2 (2%), ARA4 (4%), and ARA6 (6%)) for European sea bass (
Dicentrarchus labrax
) juveniles in order to determine (a) its effect in selected distal intestine (DI) lipid classes composition and (b) how these changes affected gut bacterial translocation rates and selected GALT-related gene expression pre and post challenge. No differences were found between distal intestines of fish fed with the graded ARA levels in total neutral lipids and total polar lipids. However, DI of fish fed with the ARA6 diet presented a higher (
P
< 0.05) level of phosphatidylethanolamine (PE) and sphingomyelin (SM) than those DI of fish fed with the ARA0.5 diet. In general terms, fatty acid profiles of DI lipid classes mirrored those of the diet dietary. Nevertheless, selective retention of ARA could be observed in glycerophospholipids when dietary levels are low (diet ARA0.5), as reflected in the higher glycerophospholipids-ARA/dietary-ARA ratio for those animals. Increased ARA dietary supplementation was inversely correlated with eicosapentaenoic acid (EPA) content in lipid classes, when data from fish fed with the diets with the same basal composition (diets ARA1 to ARA6). ARA supplementation did not affect intestinal morphometry, goblet cell number, or fish survival, in terms of gut bacterial translocation, along the challenge test. However, after the experimental infection with
Vibrio anguillarum
, the relative expression of
cox-2
and
il-1β
were upregulated (
P
< 0.05) in DI of fish fed with the diets ARA0.5 and ARA2 compared with fish fed with the rest of the experimental diets. Although dietary ARA did not affect fish survival, it altered the fatty acid composition of glycerophospholipids and the expression of pro-inflammatory genes after infection when included at the lowest concentration, which could be compromising the physical and the immune functionality of the DI, denoting the importance of ARA supplementation when low FO diets are used for marine fish.</description><identifier>ISSN: 0920-1742</identifier><identifier>EISSN: 1573-5168</identifier><identifier>DOI: 10.1007/s10695-019-00744-0</identifier><identifier>PMID: 31845079</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Animal Anatomy ; Animal Biochemistry ; Animal Physiology ; Arachidonic acid ; Biomedical and Life Sciences ; Cell number ; Cell survival ; Cyclooxygenase-2 ; Dicentrarchus labrax ; Diet ; Dietary supplements ; Digestive system ; Eicosapentaenoic acid ; Experimental infection ; Fatty acid composition ; Fatty acids ; Fish ; Fish diets ; Fish meal ; Fish oils ; Fishmeal ; Freshwater & Marine Ecology ; Gastrointestinal tract ; Gene expression ; Gut-associated lymphoid tissues ; Histology ; IL-1β ; Inflammation ; Intestine ; Intestines ; Juveniles ; Levels ; Life Sciences ; Lipids ; Marine fish ; Marine fishes ; Morphology ; Morphometry ; Nutrient deficiency ; Oils & fats ; Phosphatidylethanolamine ; Profiles ; Sea bass ; Sphingomyelin ; Survival ; Translocation ; Waterborne diseases ; Zoology</subject><ispartof>Fish physiology and biochemistry, 2020-04, Vol.46 (2), p.681-697</ispartof><rights>Springer Nature B.V. 2019</rights><rights>Fish Physiology and Biochemistry is a copyright of Springer, (2019). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c419t-e70d09dedaa599478f9c4bb063f545f48fb270606646ed613e9081b62221f5ce3</citedby><cites>FETCH-LOGICAL-c419t-e70d09dedaa599478f9c4bb063f545f48fb270606646ed613e9081b62221f5ce3</cites><orcidid>0000-0002-4358-2157</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10695-019-00744-0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10695-019-00744-0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31845079$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rivero-Ramírez, F.</creatorcontrib><creatorcontrib>Torrecillas, S.</creatorcontrib><creatorcontrib>Betancor, M. B.</creatorcontrib><creatorcontrib>Izquierdo, M. S.</creatorcontrib><creatorcontrib>Caballero, M. J.</creatorcontrib><creatorcontrib>Montero, D.</creatorcontrib><title>Effects of dietary arachidonic acid in European sea bass (Dicentrarchus labrax) distal intestine lipid classes and gut health</title><title>Fish physiology and biochemistry</title><addtitle>Fish Physiol Biochem</addtitle><addtitle>Fish Physiol Biochem</addtitle><description>The use of low fishmeal/fish oil in marine fish diets affects dietary essential fatty acids (EFAs) composition and concentration and, subsequently, may produce a marginal deficiency of those fatty acids with a direct impact on the fish intestinal physiology. Supplementation of essential fatty acids is necessary to cover the requirements of the different EFAs, including the ones belonging to the n-6 series, such as arachidonic acid (ARA). ARA, besides its structural role in the configuration of the lipid classes of the intestine, plays an important role in the functionality of the gut-associated immune tissue (GALT). The present study aimed to test five levels of dietary ARA (ARA0.5 (0.5%), ARA1 (1%), ARA2 (2%), ARA4 (4%), and ARA6 (6%)) for European sea bass (
Dicentrarchus labrax
) juveniles in order to determine (a) its effect in selected distal intestine (DI) lipid classes composition and (b) how these changes affected gut bacterial translocation rates and selected GALT-related gene expression pre and post challenge. No differences were found between distal intestines of fish fed with the graded ARA levels in total neutral lipids and total polar lipids. However, DI of fish fed with the ARA6 diet presented a higher (
P
< 0.05) level of phosphatidylethanolamine (PE) and sphingomyelin (SM) than those DI of fish fed with the ARA0.5 diet. In general terms, fatty acid profiles of DI lipid classes mirrored those of the diet dietary. Nevertheless, selective retention of ARA could be observed in glycerophospholipids when dietary levels are low (diet ARA0.5), as reflected in the higher glycerophospholipids-ARA/dietary-ARA ratio for those animals. Increased ARA dietary supplementation was inversely correlated with eicosapentaenoic acid (EPA) content in lipid classes, when data from fish fed with the diets with the same basal composition (diets ARA1 to ARA6). ARA supplementation did not affect intestinal morphometry, goblet cell number, or fish survival, in terms of gut bacterial translocation, along the challenge test. However, after the experimental infection with
Vibrio anguillarum
, the relative expression of
cox-2
and
il-1β
were upregulated (
P
< 0.05) in DI of fish fed with the diets ARA0.5 and ARA2 compared with fish fed with the rest of the experimental diets. Although dietary ARA did not affect fish survival, it altered the fatty acid composition of glycerophospholipids and the expression of pro-inflammatory genes after infection when included at the lowest concentration, which could be compromising the physical and the immune functionality of the DI, denoting the importance of ARA supplementation when low FO diets are used for marine fish.</description><subject>Animal Anatomy</subject><subject>Animal Biochemistry</subject><subject>Animal Physiology</subject><subject>Arachidonic acid</subject><subject>Biomedical and Life Sciences</subject><subject>Cell number</subject><subject>Cell survival</subject><subject>Cyclooxygenase-2</subject><subject>Dicentrarchus labrax</subject><subject>Diet</subject><subject>Dietary supplements</subject><subject>Digestive system</subject><subject>Eicosapentaenoic acid</subject><subject>Experimental infection</subject><subject>Fatty acid composition</subject><subject>Fatty acids</subject><subject>Fish</subject><subject>Fish diets</subject><subject>Fish meal</subject><subject>Fish oils</subject><subject>Fishmeal</subject><subject>Freshwater & Marine Ecology</subject><subject>Gastrointestinal tract</subject><subject>Gene expression</subject><subject>Gut-associated lymphoid tissues</subject><subject>Histology</subject><subject>IL-1β</subject><subject>Inflammation</subject><subject>Intestine</subject><subject>Intestines</subject><subject>Juveniles</subject><subject>Levels</subject><subject>Life Sciences</subject><subject>Lipids</subject><subject>Marine fish</subject><subject>Marine fishes</subject><subject>Morphology</subject><subject>Morphometry</subject><subject>Nutrient deficiency</subject><subject>Oils & fats</subject><subject>Phosphatidylethanolamine</subject><subject>Profiles</subject><subject>Sea bass</subject><subject>Sphingomyelin</subject><subject>Survival</subject><subject>Translocation</subject><subject>Waterborne diseases</subject><subject>Zoology</subject><issn>0920-1742</issn><issn>1573-5168</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kU1vFSEUhomxsdfqH3BhSNzUxdQDw8ewNPX6kTTppq4Jwxx6aeYyV2ASu_C_S71VExeuCOF5n3PCS8grBhcMQL8rDJSRHTDTtasQHTwhGyZ130mmhqdkA4ZDx7Tgp-R5KXcAYLRiz8hpzwYhQZsN-bENAX0tdAl0ilhdvqcuO7-L05Kip87HicZEt2teDugSLejo6Eqh5x-ix1Szy363Fjq7Mbvvb5ukVDe3SMVSY0I6x0NT-LllsFCXJnq7VrpDN9fdC3IS3Fzw5eN5Rr5-3N5cfu6urj99uXx_1XnBTO1QwwRmwsk5aYzQQzBejCOoPkghgxjCyDUoUEoonBTr0cDARsU5Z0F67M_I-dF7yMu3tS1m97F4nGeXcFmL5T3Xpo0CaOibf9C7Zc2pbdcozZhRQstG8SPl81JKxmAPOe7b51kG9qEceyzHtnLsr3Lsg_r1o3od9zj9ifxuowH9ESjtKd1i_jv7P9qffhaaIg</recordid><startdate>20200401</startdate><enddate>20200401</enddate><creator>Rivero-Ramírez, F.</creator><creator>Torrecillas, S.</creator><creator>Betancor, M. B.</creator><creator>Izquierdo, M. S.</creator><creator>Caballero, M. J.</creator><creator>Montero, D.</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QH</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TM</scope><scope>7TN</scope><scope>7U7</scope><scope>7UA</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H95</scope><scope>H98</scope><scope>H99</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L.F</scope><scope>L.G</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PCBAR</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-4358-2157</orcidid></search><sort><creationdate>20200401</creationdate><title>Effects of dietary arachidonic acid in European sea bass (Dicentrarchus labrax) distal intestine lipid classes and gut health</title><author>Rivero-Ramírez, F. ; Torrecillas, S. ; Betancor, M. B. ; Izquierdo, M. S. ; Caballero, M. J. ; Montero, D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c419t-e70d09dedaa599478f9c4bb063f545f48fb270606646ed613e9081b62221f5ce3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Animal Anatomy</topic><topic>Animal Biochemistry</topic><topic>Animal Physiology</topic><topic>Arachidonic acid</topic><topic>Biomedical and Life Sciences</topic><topic>Cell number</topic><topic>Cell survival</topic><topic>Cyclooxygenase-2</topic><topic>Dicentrarchus labrax</topic><topic>Diet</topic><topic>Dietary supplements</topic><topic>Digestive system</topic><topic>Eicosapentaenoic acid</topic><topic>Experimental infection</topic><topic>Fatty acid composition</topic><topic>Fatty acids</topic><topic>Fish</topic><topic>Fish diets</topic><topic>Fish meal</topic><topic>Fish oils</topic><topic>Fishmeal</topic><topic>Freshwater & Marine Ecology</topic><topic>Gastrointestinal tract</topic><topic>Gene expression</topic><topic>Gut-associated lymphoid tissues</topic><topic>Histology</topic><topic>IL-1β</topic><topic>Inflammation</topic><topic>Intestine</topic><topic>Intestines</topic><topic>Juveniles</topic><topic>Levels</topic><topic>Life Sciences</topic><topic>Lipids</topic><topic>Marine fish</topic><topic>Marine fishes</topic><topic>Morphology</topic><topic>Morphometry</topic><topic>Nutrient deficiency</topic><topic>Oils & fats</topic><topic>Phosphatidylethanolamine</topic><topic>Profiles</topic><topic>Sea bass</topic><topic>Sphingomyelin</topic><topic>Survival</topic><topic>Translocation</topic><topic>Waterborne diseases</topic><topic>Zoology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rivero-Ramírez, F.</creatorcontrib><creatorcontrib>Torrecillas, S.</creatorcontrib><creatorcontrib>Betancor, M. B.</creatorcontrib><creatorcontrib>Izquierdo, M. S.</creatorcontrib><creatorcontrib>Caballero, M. J.</creatorcontrib><creatorcontrib>Montero, D.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Aqualine</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oceanic Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Water Resources Abstracts</collection><collection>ProQuest_Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>Earth, Atmospheric & Aquatic Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Earth, Atmospheric & Aquatic Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Fish physiology and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rivero-Ramírez, F.</au><au>Torrecillas, S.</au><au>Betancor, M. B.</au><au>Izquierdo, M. S.</au><au>Caballero, M. J.</au><au>Montero, D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of dietary arachidonic acid in European sea bass (Dicentrarchus labrax) distal intestine lipid classes and gut health</atitle><jtitle>Fish physiology and biochemistry</jtitle><stitle>Fish Physiol Biochem</stitle><addtitle>Fish Physiol Biochem</addtitle><date>2020-04-01</date><risdate>2020</risdate><volume>46</volume><issue>2</issue><spage>681</spage><epage>697</epage><pages>681-697</pages><issn>0920-1742</issn><eissn>1573-5168</eissn><abstract>The use of low fishmeal/fish oil in marine fish diets affects dietary essential fatty acids (EFAs) composition and concentration and, subsequently, may produce a marginal deficiency of those fatty acids with a direct impact on the fish intestinal physiology. Supplementation of essential fatty acids is necessary to cover the requirements of the different EFAs, including the ones belonging to the n-6 series, such as arachidonic acid (ARA). ARA, besides its structural role in the configuration of the lipid classes of the intestine, plays an important role in the functionality of the gut-associated immune tissue (GALT). The present study aimed to test five levels of dietary ARA (ARA0.5 (0.5%), ARA1 (1%), ARA2 (2%), ARA4 (4%), and ARA6 (6%)) for European sea bass (
Dicentrarchus labrax
) juveniles in order to determine (a) its effect in selected distal intestine (DI) lipid classes composition and (b) how these changes affected gut bacterial translocation rates and selected GALT-related gene expression pre and post challenge. No differences were found between distal intestines of fish fed with the graded ARA levels in total neutral lipids and total polar lipids. However, DI of fish fed with the ARA6 diet presented a higher (
P
< 0.05) level of phosphatidylethanolamine (PE) and sphingomyelin (SM) than those DI of fish fed with the ARA0.5 diet. In general terms, fatty acid profiles of DI lipid classes mirrored those of the diet dietary. Nevertheless, selective retention of ARA could be observed in glycerophospholipids when dietary levels are low (diet ARA0.5), as reflected in the higher glycerophospholipids-ARA/dietary-ARA ratio for those animals. Increased ARA dietary supplementation was inversely correlated with eicosapentaenoic acid (EPA) content in lipid classes, when data from fish fed with the diets with the same basal composition (diets ARA1 to ARA6). ARA supplementation did not affect intestinal morphometry, goblet cell number, or fish survival, in terms of gut bacterial translocation, along the challenge test. However, after the experimental infection with
Vibrio anguillarum
, the relative expression of
cox-2
and
il-1β
were upregulated (
P
< 0.05) in DI of fish fed with the diets ARA0.5 and ARA2 compared with fish fed with the rest of the experimental diets. Although dietary ARA did not affect fish survival, it altered the fatty acid composition of glycerophospholipids and the expression of pro-inflammatory genes after infection when included at the lowest concentration, which could be compromising the physical and the immune functionality of the DI, denoting the importance of ARA supplementation when low FO diets are used for marine fish.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>31845079</pmid><doi>10.1007/s10695-019-00744-0</doi><tpages>17</tpages><orcidid>https://orcid.org/0000-0002-4358-2157</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0920-1742 |
| ispartof | Fish physiology and biochemistry, 2020-04, Vol.46 (2), p.681-697 |
| issn | 0920-1742 1573-5168 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2327941900 |
| source | SpringerLink_现刊 |
| subjects | Animal Anatomy Animal Biochemistry Animal Physiology Arachidonic acid Biomedical and Life Sciences Cell number Cell survival Cyclooxygenase-2 Dicentrarchus labrax Diet Dietary supplements Digestive system Eicosapentaenoic acid Experimental infection Fatty acid composition Fatty acids Fish Fish diets Fish meal Fish oils Fishmeal Freshwater & Marine Ecology Gastrointestinal tract Gene expression Gut-associated lymphoid tissues Histology IL-1β Inflammation Intestine Intestines Juveniles Levels Life Sciences Lipids Marine fish Marine fishes Morphology Morphometry Nutrient deficiency Oils & fats Phosphatidylethanolamine Profiles Sea bass Sphingomyelin Survival Translocation Waterborne diseases Zoology |
| title | Effects of dietary arachidonic acid in European sea bass (Dicentrarchus labrax) distal intestine lipid classes and gut health |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-28T07%3A50%3A29IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effects%20of%20dietary%20arachidonic%20acid%20in%20European%20sea%20bass%20(Dicentrarchus%20labrax)%20distal%20intestine%20lipid%20classes%20and%20gut%20health&rft.jtitle=Fish%20physiology%20and%20biochemistry&rft.au=Rivero-Ram%C3%ADrez,%20F.&rft.date=2020-04-01&rft.volume=46&rft.issue=2&rft.spage=681&rft.epage=697&rft.pages=681-697&rft.issn=0920-1742&rft.eissn=1573-5168&rft_id=info:doi/10.1007/s10695-019-00744-0&rft_dat=%3Cproquest_cross%3E2371196475%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2371196475&rft_id=info:pmid/31845079&rfr_iscdi=true |