Rapid strand replacement primer thermostat visual sensor based on Bst DNA polymerase and pyrophosphatase for detecting Vibrio parahaemolyticus

•A strand replacement primer thermostat phosphate (SRPP) sensor is designed.•SRP facilitates chain substitution and opens a hairpin with it added at its 3′ end.•The amplification is within 35 min in the manner of thermostat amplification.•Phosphate from the by-product PPi triggers phosphomolybdate b...

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Veröffentlicht in:	Food chemistry 2020-04, Vol.310, p.125955-125955, Article 125955
Hauptverfasser:	Li, Xuetong, Su, Yuan, Chu, Huashuo, Lyu, Shuxia, Tian, Jingjing, Xu, Wentao
Format:	Artikel
Sprache:	eng
Schlagworte:	DNA Primers - genetics DNA-Directed DNA Polymerase - genetics Food Analysis - instrumentation Food Analysis - methods Food Microbiology - methods Phosphate Pyrophosphatase Pyrophosphatases - genetics Sensitivity and Specificity Strand replacement primer Vibrio parahaemolyticus Vibrio parahaemolyticus - genetics Visualization
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