Comparison of oxidative stress induced by clarithromycin in two freshwater microalgae Raphidocelis subcapitata and Chlorella vulgaris
•Two microalgae R. subcapitata and C. vulgaris were used to test clarithromycin.•Effects of clarithromycin on the growth, oxidative stress, and photosynthetic pigments of two algae were compared.•R. subcapitata was more sensitive than C. vulgaris when exposure to clarithromycin.•SOD, CAT, and GP in...
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| Veröffentlicht in: | Aquatic toxicology 2020-02, Vol.219, p.105376-105376, Article 105376 |
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| creator | Guo, Jiahua Peng, Jianglin Lei, Yuan Kanerva, Mirella Li, Qi Song, Jinxi Guo, Jipu Sun, Haotian |
| description | •Two microalgae R. subcapitata and C. vulgaris were used to test clarithromycin.•Effects of clarithromycin on the growth, oxidative stress, and photosynthetic pigments of two algae were compared.•R. subcapitata was more sensitive than C. vulgaris when exposure to clarithromycin.•SOD, CAT, and GP in R. subcapitata and C. vulgaris were increased in a concentration-dependent manner.
Clarithromycin (CLA), a macrolide antibiotic, has been frequently detected in the global surface waters. Concerns have been raised over the potential impacts of CLA on the non-target aquatic species, particularly algae acting as the primary producers in the ecosystem. This study therefore evaluated the toxicological effects of CLA at a range of concentration levels (0, 5, 20, 40, 80 μg L−1) on two green algae, Raphidocelis subcapitata (R. subcapitata) and Chlorella vulgaris (C. vulgaris). The algal growth, photosynthetic pigment contents, lipid peroxidation biomarker malondialdehyde (MDA), responses of antioxidants including superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), glutathione peroxidase (GP), and glutathione S-transferase (GST) were measured. After 7 d exposure, the growth of R. subcapitata was inhibited with the CLA exposure levels higher than 20 μg L−1, whereas the inhibition in C. vulgaris was detected at the concentration level of 80 μg L−1. The MDA contents in both species were elevated. To cope with the increased levels of ROS, the activities of enzymatic antioxidants (SOD, CAT, GP, and GST) and the content of non-enzymatic antioxidant (GSH) in R. subcapitata were all enhanced. However, in C. vulgaris, enhancement was detected only in the activities of antioxidant enzymes (SOD, CAT, and GP). In addition, chlorophyll a, b, and carotenoid contents were all significantly increased in R. subcapitata but decreased in C. vulgaris. The results suggested that R. subcapitata is more sensitive to CLA exposure than C. vulgaris. This study provides insights into the CLA - oxidative stress process in two algae. |
| doi_str_mv | 10.1016/j.aquatox.2019.105376 |
| format | Article |
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Clarithromycin (CLA), a macrolide antibiotic, has been frequently detected in the global surface waters. Concerns have been raised over the potential impacts of CLA on the non-target aquatic species, particularly algae acting as the primary producers in the ecosystem. This study therefore evaluated the toxicological effects of CLA at a range of concentration levels (0, 5, 20, 40, 80 μg L−1) on two green algae, Raphidocelis subcapitata (R. subcapitata) and Chlorella vulgaris (C. vulgaris). The algal growth, photosynthetic pigment contents, lipid peroxidation biomarker malondialdehyde (MDA), responses of antioxidants including superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), glutathione peroxidase (GP), and glutathione S-transferase (GST) were measured. After 7 d exposure, the growth of R. subcapitata was inhibited with the CLA exposure levels higher than 20 μg L−1, whereas the inhibition in C. vulgaris was detected at the concentration level of 80 μg L−1. The MDA contents in both species were elevated. To cope with the increased levels of ROS, the activities of enzymatic antioxidants (SOD, CAT, GP, and GST) and the content of non-enzymatic antioxidant (GSH) in R. subcapitata were all enhanced. However, in C. vulgaris, enhancement was detected only in the activities of antioxidant enzymes (SOD, CAT, and GP). In addition, chlorophyll a, b, and carotenoid contents were all significantly increased in R. subcapitata but decreased in C. vulgaris. The results suggested that R. subcapitata is more sensitive to CLA exposure than C. vulgaris. This study provides insights into the CLA - oxidative stress process in two algae.</description><identifier>ISSN: 0166-445X</identifier><identifier>EISSN: 1879-1514</identifier><identifier>DOI: 10.1016/j.aquatox.2019.105376</identifier><identifier>PMID: 31838304</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Antibiotic ; Antioxidant system ; Antioxidants - metabolism ; Catalase - pharmacology ; Chlorella vulgaris - drug effects ; Chlorella vulgaris - metabolism ; Chlorophyll A - metabolism ; Clarithromycin - toxicity ; Ecosystem ; Fresh Water - chemistry ; Glutathione - metabolism ; Growth inhibition ; Lipid peroxidation ; Lipid Peroxidation - drug effects ; Malondialdehyde - metabolism ; Microalgae - drug effects ; Microalgae - metabolism ; Oxidative Stress - drug effects ; Photosynthesis - drug effects ; Photosynthetic pigments ; Superoxide Dismutase - metabolism ; Water Pollutants, Chemical - toxicity</subject><ispartof>Aquatic toxicology, 2020-02, Vol.219, p.105376-105376, Article 105376</ispartof><rights>2019 Elsevier B.V.</rights><rights>Copyright © 2019 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c280t-49ce0736cf73ae9571272964a8a13fa2a8d2fe3be682aeb3b7be1e14cca710d73</citedby><cites>FETCH-LOGICAL-c280t-49ce0736cf73ae9571272964a8a13fa2a8d2fe3be682aeb3b7be1e14cca710d73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.aquatox.2019.105376$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31838304$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Guo, Jiahua</creatorcontrib><creatorcontrib>Peng, Jianglin</creatorcontrib><creatorcontrib>Lei, Yuan</creatorcontrib><creatorcontrib>Kanerva, Mirella</creatorcontrib><creatorcontrib>Li, Qi</creatorcontrib><creatorcontrib>Song, Jinxi</creatorcontrib><creatorcontrib>Guo, Jipu</creatorcontrib><creatorcontrib>Sun, Haotian</creatorcontrib><title>Comparison of oxidative stress induced by clarithromycin in two freshwater microalgae Raphidocelis subcapitata and Chlorella vulgaris</title><title>Aquatic toxicology</title><addtitle>Aquat Toxicol</addtitle><description>•Two microalgae R. subcapitata and C. vulgaris were used to test clarithromycin.•Effects of clarithromycin on the growth, oxidative stress, and photosynthetic pigments of two algae were compared.•R. subcapitata was more sensitive than C. vulgaris when exposure to clarithromycin.•SOD, CAT, and GP in R. subcapitata and C. vulgaris were increased in a concentration-dependent manner.
Clarithromycin (CLA), a macrolide antibiotic, has been frequently detected in the global surface waters. Concerns have been raised over the potential impacts of CLA on the non-target aquatic species, particularly algae acting as the primary producers in the ecosystem. This study therefore evaluated the toxicological effects of CLA at a range of concentration levels (0, 5, 20, 40, 80 μg L−1) on two green algae, Raphidocelis subcapitata (R. subcapitata) and Chlorella vulgaris (C. vulgaris). The algal growth, photosynthetic pigment contents, lipid peroxidation biomarker malondialdehyde (MDA), responses of antioxidants including superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), glutathione peroxidase (GP), and glutathione S-transferase (GST) were measured. After 7 d exposure, the growth of R. subcapitata was inhibited with the CLA exposure levels higher than 20 μg L−1, whereas the inhibition in C. vulgaris was detected at the concentration level of 80 μg L−1. The MDA contents in both species were elevated. To cope with the increased levels of ROS, the activities of enzymatic antioxidants (SOD, CAT, GP, and GST) and the content of non-enzymatic antioxidant (GSH) in R. subcapitata were all enhanced. However, in C. vulgaris, enhancement was detected only in the activities of antioxidant enzymes (SOD, CAT, and GP). In addition, chlorophyll a, b, and carotenoid contents were all significantly increased in R. subcapitata but decreased in C. vulgaris. The results suggested that R. subcapitata is more sensitive to CLA exposure than C. vulgaris. This study provides insights into the CLA - oxidative stress process in two algae.</description><subject>Antibiotic</subject><subject>Antioxidant system</subject><subject>Antioxidants - metabolism</subject><subject>Catalase - pharmacology</subject><subject>Chlorella vulgaris - drug effects</subject><subject>Chlorella vulgaris - metabolism</subject><subject>Chlorophyll A - metabolism</subject><subject>Clarithromycin - toxicity</subject><subject>Ecosystem</subject><subject>Fresh Water - chemistry</subject><subject>Glutathione - metabolism</subject><subject>Growth inhibition</subject><subject>Lipid peroxidation</subject><subject>Lipid Peroxidation - drug effects</subject><subject>Malondialdehyde - metabolism</subject><subject>Microalgae - drug effects</subject><subject>Microalgae - metabolism</subject><subject>Oxidative Stress - drug effects</subject><subject>Photosynthesis - drug effects</subject><subject>Photosynthetic pigments</subject><subject>Superoxide Dismutase - metabolism</subject><subject>Water Pollutants, Chemical - toxicity</subject><issn>0166-445X</issn><issn>1879-1514</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc9u1DAQxi0EokvhEUA-csniP0mcnBBaFahUqVJFpd6siT1hvUri1Ha23QfgvfFql17xxZLnN_5mvo-Qj5ytOeP1l90aHhdI_nktGG_zWyVV_YqseKPagle8fE1WmauLsqweLsi7GHcsH1G2b8mF5I1sJCtX5M_GjzMEF_1EfU_9s7OQ3B5pTAFjpG6yi0FLuwM1Q-bSNvjxYNyUKzQ9edpnbPsECQMdnQkeht-A9A7mrbPe4OAijUtnYHYJElCYLN1sBx9wGIDul0xn8ffkTQ9DxA_n-5Lcf7_6tflZ3Nz-uN58uymMaFgqytYgU7I2vZKAbaW4UKKtS2iAyx4ENFb0KDusGwHYyU51yJGXxoDizCp5ST6f_p2Df1wwJj26aI6jTOiXqIUUSjaCtzyj1QnNO8UYsNdzcCOEg-ZMHxPQO31OQB8T0KcEct-ns8TSjWhfuv5ZnoGvJwDzonuHQUfjcMomu4AmaevdfyT-AhdYnmc</recordid><startdate>202002</startdate><enddate>202002</enddate><creator>Guo, Jiahua</creator><creator>Peng, Jianglin</creator><creator>Lei, Yuan</creator><creator>Kanerva, Mirella</creator><creator>Li, Qi</creator><creator>Song, Jinxi</creator><creator>Guo, Jipu</creator><creator>Sun, Haotian</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>202002</creationdate><title>Comparison of oxidative stress induced by clarithromycin in two freshwater microalgae Raphidocelis subcapitata and Chlorella vulgaris</title><author>Guo, Jiahua ; Peng, Jianglin ; Lei, Yuan ; Kanerva, Mirella ; Li, Qi ; Song, Jinxi ; Guo, Jipu ; Sun, Haotian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c280t-49ce0736cf73ae9571272964a8a13fa2a8d2fe3be682aeb3b7be1e14cca710d73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Antibiotic</topic><topic>Antioxidant system</topic><topic>Antioxidants - metabolism</topic><topic>Catalase - pharmacology</topic><topic>Chlorella vulgaris - drug effects</topic><topic>Chlorella vulgaris - metabolism</topic><topic>Chlorophyll A - metabolism</topic><topic>Clarithromycin - toxicity</topic><topic>Ecosystem</topic><topic>Fresh Water - chemistry</topic><topic>Glutathione - metabolism</topic><topic>Growth inhibition</topic><topic>Lipid peroxidation</topic><topic>Lipid Peroxidation - drug effects</topic><topic>Malondialdehyde - metabolism</topic><topic>Microalgae - drug effects</topic><topic>Microalgae - metabolism</topic><topic>Oxidative Stress - drug effects</topic><topic>Photosynthesis - drug effects</topic><topic>Photosynthetic pigments</topic><topic>Superoxide Dismutase - metabolism</topic><topic>Water Pollutants, Chemical - toxicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Guo, Jiahua</creatorcontrib><creatorcontrib>Peng, Jianglin</creatorcontrib><creatorcontrib>Lei, Yuan</creatorcontrib><creatorcontrib>Kanerva, Mirella</creatorcontrib><creatorcontrib>Li, Qi</creatorcontrib><creatorcontrib>Song, Jinxi</creatorcontrib><creatorcontrib>Guo, Jipu</creatorcontrib><creatorcontrib>Sun, Haotian</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Aquatic toxicology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Guo, Jiahua</au><au>Peng, Jianglin</au><au>Lei, Yuan</au><au>Kanerva, Mirella</au><au>Li, Qi</au><au>Song, Jinxi</au><au>Guo, Jipu</au><au>Sun, Haotian</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of oxidative stress induced by clarithromycin in two freshwater microalgae Raphidocelis subcapitata and Chlorella vulgaris</atitle><jtitle>Aquatic toxicology</jtitle><addtitle>Aquat Toxicol</addtitle><date>2020-02</date><risdate>2020</risdate><volume>219</volume><spage>105376</spage><epage>105376</epage><pages>105376-105376</pages><artnum>105376</artnum><issn>0166-445X</issn><eissn>1879-1514</eissn><abstract>•Two microalgae R. subcapitata and C. vulgaris were used to test clarithromycin.•Effects of clarithromycin on the growth, oxidative stress, and photosynthetic pigments of two algae were compared.•R. subcapitata was more sensitive than C. vulgaris when exposure to clarithromycin.•SOD, CAT, and GP in R. subcapitata and C. vulgaris were increased in a concentration-dependent manner.
Clarithromycin (CLA), a macrolide antibiotic, has been frequently detected in the global surface waters. Concerns have been raised over the potential impacts of CLA on the non-target aquatic species, particularly algae acting as the primary producers in the ecosystem. This study therefore evaluated the toxicological effects of CLA at a range of concentration levels (0, 5, 20, 40, 80 μg L−1) on two green algae, Raphidocelis subcapitata (R. subcapitata) and Chlorella vulgaris (C. vulgaris). The algal growth, photosynthetic pigment contents, lipid peroxidation biomarker malondialdehyde (MDA), responses of antioxidants including superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), glutathione peroxidase (GP), and glutathione S-transferase (GST) were measured. After 7 d exposure, the growth of R. subcapitata was inhibited with the CLA exposure levels higher than 20 μg L−1, whereas the inhibition in C. vulgaris was detected at the concentration level of 80 μg L−1. The MDA contents in both species were elevated. To cope with the increased levels of ROS, the activities of enzymatic antioxidants (SOD, CAT, GP, and GST) and the content of non-enzymatic antioxidant (GSH) in R. subcapitata were all enhanced. However, in C. vulgaris, enhancement was detected only in the activities of antioxidant enzymes (SOD, CAT, and GP). In addition, chlorophyll a, b, and carotenoid contents were all significantly increased in R. subcapitata but decreased in C. vulgaris. The results suggested that R. subcapitata is more sensitive to CLA exposure than C. vulgaris. This study provides insights into the CLA - oxidative stress process in two algae.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>31838304</pmid><doi>10.1016/j.aquatox.2019.105376</doi><tpages>1</tpages></addata></record> |
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| subjects | Antibiotic Antioxidant system Antioxidants - metabolism Catalase - pharmacology Chlorella vulgaris - drug effects Chlorella vulgaris - metabolism Chlorophyll A - metabolism Clarithromycin - toxicity Ecosystem Fresh Water - chemistry Glutathione - metabolism Growth inhibition Lipid peroxidation Lipid Peroxidation - drug effects Malondialdehyde - metabolism Microalgae - drug effects Microalgae - metabolism Oxidative Stress - drug effects Photosynthesis - drug effects Photosynthetic pigments Superoxide Dismutase - metabolism Water Pollutants, Chemical - toxicity |
| title | Comparison of oxidative stress induced by clarithromycin in two freshwater microalgae Raphidocelis subcapitata and Chlorella vulgaris |
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