Identification and functional characterization of squalene epoxidases and oxidosqualene cyclases from Tripterygium wilfordii

Key message We cloned two squalene epoxidases and five oxidosqualene cyclases, and identified their function using CRISPR/Cas9 tool and yeast heterologous expression. Triterpenes are the main active ingredients of Tripterygium wilfordii Hook.f., a traditional Chinese medicinal plant with many encour...

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Veröffentlicht in:	Plant cell reports 2020-03, Vol.39 (3), p.409-418
Hauptverfasser:	Liu, Yuan, Zhou, Jiawei, Hu, Tianyuan, Lu, Yun, Gao, Linhui, Tu, Lichan, Gao, Jie, Huang, Luqi, Gao, Wei
Format:	Artikel
Sprache:	eng
Schlagworte:	Biomedical and Life Sciences Biosynthesis Biosynthetic Pathways - genetics Biotechnology Cell Biology Chassis Complementation CRISPR Disruption Fermentation Gene expression Gene Expression Regulation, Plant Genes Genes, Plant Herbal medicine Intramolecular Transferases - metabolism Life Sciences Medicinal plants Mutants Original Article Phylogeny Plant Biochemistry Plant Sciences Saccharomyces cerevisiae - metabolism Squalene Squalene Monooxygenase - metabolism Sterols - chemistry Sterols - metabolism Tripterygium - enzymology Tripterygium - genetics Tripterygium wilfordii Triterpenes Triterpenes - chemistry Triterpenes - metabolism Yeast Yeasts
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