Fabrication of Piperazine Functionalized Polymeric Monolithic Tip for Rapid Enrichment of Glycopeptides/Glycans
Enrichment strategies are designed for the pretreatment of low-abundance glycans and glycopeptides prior to mass spectrometric (MS) analysis. Here, a tip-based strategy is being reported for the enrichment of glycopeptides and glycans using a piperazine modified polymeric monolithic tip. The tip is...
Gespeichert in:
| Veröffentlicht in: | Analytical chemistry (Washington) 2020-01, Vol.92 (1), p.683-689 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 689 |
|---|---|
| container_issue | 1 |
| container_start_page | 683 |
| container_title | Analytical chemistry (Washington) |
| container_volume | 92 |
| creator | Sajid, Muhammad Salman Jovcevski, Blagojce Pukala, Tara Louise Jabeen, Fahmida Najam-ul-Haq, Muhammad |
| description | Enrichment strategies are designed for the pretreatment of low-abundance glycans and glycopeptides prior to mass spectrometric (MS) analysis. Here, a tip-based strategy is being reported for the enrichment of glycopeptides and glycans using a piperazine modified polymeric monolithic tip. The tip is fabricated using the free radical polymerization. Fast separation (2 min) is achieved under optimized conditions with 20 cycles per step of loading, incubation, washing, and elution followed by MALDI-MS analysis. A total of 25, 22, and 34 glycopeptides covering all glycosylation sites are enriched by the modified tips from tryptic digests of horse radish peroxidase, chicken avidin, and human immunoglobulin G, respectively. Piperazine exhibits high selectivity 1:400 horse radish peroxidase/bovine serum albumin, sensitivity to 100 attomoles, recovery 89.51%, and batch to batch reproducibility (RSD > 1) in glycopeptides enrichment. Piperazine tips also enrich glycans from ovalbumin and human immunoglobulin G. High selectivity (1:1200, ovalbumin/BSA) and detection limit of 100 attomole is attained for glycans and furthermore 58 glycans are enriched from human serum. Thus, piperazine tips can be used as an enrichment tool for swift, cost-effective routine analysis of biological samples for separation of glycopeptides and glycans. |
| doi_str_mv | 10.1021/acs.analchem.9b02068 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2327372957</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2327372957</sourcerecordid><originalsourceid>FETCH-LOGICAL-a376t-791bb6464524badc26cbd4f8c3cac6a302fff44ecdcf60c531e85fc3672f36373</originalsourceid><addsrcrecordid>eNp9kc1LwzAchoMoOqf_gUjAi5duv3w07Y4iToWJInouaZqwSJvUpD1sf70t2zx48JSv531_hAehKwIzApTMpYoz6WSt1rqZLUqgIPIjNCEphUTkOT1GEwBgCc0AztB5jF8AhAARp-iMkZzDImcT5JeyDFbJznqHvcFvttVBbq3TeNk7NV7L2m51hd98vWn0wOIX73xtu_Ww_bAtNj7gd9naCj-44XndaNeNVY_1RvlWt52tdJyPJ-niBToxso76cr9O0efy4eP-KVm9Pj7f360SyTLRJdmClKXggqeUl7JSVKiy4iZXTEklJANqjOFcq0oZASplROepUUxk1DDBMjZFt7veNvjvXseuaGxUuq6l076PBWU0YxldpCN68wf98n0Yvj1SLBWCszQfKL6jVPAxBm2KNthGhk1BoBiFFIOQ4iCk2AsZYtf78r5sdPUbOhgYANgBY_x38L-dPzb5nA4</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2335664358</pqid></control><display><type>article</type><title>Fabrication of Piperazine Functionalized Polymeric Monolithic Tip for Rapid Enrichment of Glycopeptides/Glycans</title><source>MEDLINE</source><source>ACS Publications</source><creator>Sajid, Muhammad Salman ; Jovcevski, Blagojce ; Pukala, Tara Louise ; Jabeen, Fahmida ; Najam-ul-Haq, Muhammad</creator><creatorcontrib>Sajid, Muhammad Salman ; Jovcevski, Blagojce ; Pukala, Tara Louise ; Jabeen, Fahmida ; Najam-ul-Haq, Muhammad</creatorcontrib><description>Enrichment strategies are designed for the pretreatment of low-abundance glycans and glycopeptides prior to mass spectrometric (MS) analysis. Here, a tip-based strategy is being reported for the enrichment of glycopeptides and glycans using a piperazine modified polymeric monolithic tip. The tip is fabricated using the free radical polymerization. Fast separation (2 min) is achieved under optimized conditions with 20 cycles per step of loading, incubation, washing, and elution followed by MALDI-MS analysis. A total of 25, 22, and 34 glycopeptides covering all glycosylation sites are enriched by the modified tips from tryptic digests of horse radish peroxidase, chicken avidin, and human immunoglobulin G, respectively. Piperazine exhibits high selectivity 1:400 horse radish peroxidase/bovine serum albumin, sensitivity to 100 attomoles, recovery 89.51%, and batch to batch reproducibility (RSD > 1) in glycopeptides enrichment. Piperazine tips also enrich glycans from ovalbumin and human immunoglobulin G. High selectivity (1:1200, ovalbumin/BSA) and detection limit of 100 attomole is attained for glycans and furthermore 58 glycans are enriched from human serum. Thus, piperazine tips can be used as an enrichment tool for swift, cost-effective routine analysis of biological samples for separation of glycopeptides and glycans.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/acs.analchem.9b02068</identifier><identifier>PMID: 31840983</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Animals ; Antibiotics ; Avidin ; Biological properties ; Biological samples ; Bovine serum albumin ; Cattle ; Chemistry ; Cost analysis ; Elution ; Enrichment ; Fabrication ; Free radical polymerization ; Free radicals ; Glycopeptides ; Glycopeptides - blood ; Glycopeptides - metabolism ; Glycosylation ; Horseradish Peroxidase - chemistry ; Horseradish Peroxidase - metabolism ; Horses ; Humans ; IgG antibody ; Immunoglobulin G ; Immunoglobulins ; Ovalbumin ; Peroxidase ; Piperazine ; Polymerization ; Polymers - chemistry ; Polysaccharides ; Polysaccharides - blood ; Polysaccharides - metabolism ; Poultry ; Pretreatment ; Radishes ; Selectivity ; Separation ; Serum albumin ; Serum Albumin, Bovine - chemistry ; Serum Albumin, Bovine - metabolism ; Spectrometry ; Tips</subject><ispartof>Analytical chemistry (Washington), 2020-01, Vol.92 (1), p.683-689</ispartof><rights>Copyright American Chemical Society Jan 7, 2020</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a376t-791bb6464524badc26cbd4f8c3cac6a302fff44ecdcf60c531e85fc3672f36373</citedby><cites>FETCH-LOGICAL-a376t-791bb6464524badc26cbd4f8c3cac6a302fff44ecdcf60c531e85fc3672f36373</cites><orcidid>0000-0002-2090-5072 ; 0000-0001-7999-1385 ; 0000-0001-7391-1436</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.analchem.9b02068$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.analchem.9b02068$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,777,781,2752,27057,27905,27906,56719,56769</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31840983$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sajid, Muhammad Salman</creatorcontrib><creatorcontrib>Jovcevski, Blagojce</creatorcontrib><creatorcontrib>Pukala, Tara Louise</creatorcontrib><creatorcontrib>Jabeen, Fahmida</creatorcontrib><creatorcontrib>Najam-ul-Haq, Muhammad</creatorcontrib><title>Fabrication of Piperazine Functionalized Polymeric Monolithic Tip for Rapid Enrichment of Glycopeptides/Glycans</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. Chem</addtitle><description>Enrichment strategies are designed for the pretreatment of low-abundance glycans and glycopeptides prior to mass spectrometric (MS) analysis. Here, a tip-based strategy is being reported for the enrichment of glycopeptides and glycans using a piperazine modified polymeric monolithic tip. The tip is fabricated using the free radical polymerization. Fast separation (2 min) is achieved under optimized conditions with 20 cycles per step of loading, incubation, washing, and elution followed by MALDI-MS analysis. A total of 25, 22, and 34 glycopeptides covering all glycosylation sites are enriched by the modified tips from tryptic digests of horse radish peroxidase, chicken avidin, and human immunoglobulin G, respectively. Piperazine exhibits high selectivity 1:400 horse radish peroxidase/bovine serum albumin, sensitivity to 100 attomoles, recovery 89.51%, and batch to batch reproducibility (RSD > 1) in glycopeptides enrichment. Piperazine tips also enrich glycans from ovalbumin and human immunoglobulin G. High selectivity (1:1200, ovalbumin/BSA) and detection limit of 100 attomole is attained for glycans and furthermore 58 glycans are enriched from human serum. Thus, piperazine tips can be used as an enrichment tool for swift, cost-effective routine analysis of biological samples for separation of glycopeptides and glycans.</description><subject>Animals</subject><subject>Antibiotics</subject><subject>Avidin</subject><subject>Biological properties</subject><subject>Biological samples</subject><subject>Bovine serum albumin</subject><subject>Cattle</subject><subject>Chemistry</subject><subject>Cost analysis</subject><subject>Elution</subject><subject>Enrichment</subject><subject>Fabrication</subject><subject>Free radical polymerization</subject><subject>Free radicals</subject><subject>Glycopeptides</subject><subject>Glycopeptides - blood</subject><subject>Glycopeptides - metabolism</subject><subject>Glycosylation</subject><subject>Horseradish Peroxidase - chemistry</subject><subject>Horseradish Peroxidase - metabolism</subject><subject>Horses</subject><subject>Humans</subject><subject>IgG antibody</subject><subject>Immunoglobulin G</subject><subject>Immunoglobulins</subject><subject>Ovalbumin</subject><subject>Peroxidase</subject><subject>Piperazine</subject><subject>Polymerization</subject><subject>Polymers - chemistry</subject><subject>Polysaccharides</subject><subject>Polysaccharides - blood</subject><subject>Polysaccharides - metabolism</subject><subject>Poultry</subject><subject>Pretreatment</subject><subject>Radishes</subject><subject>Selectivity</subject><subject>Separation</subject><subject>Serum albumin</subject><subject>Serum Albumin, Bovine - chemistry</subject><subject>Serum Albumin, Bovine - metabolism</subject><subject>Spectrometry</subject><subject>Tips</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc1LwzAchoMoOqf_gUjAi5duv3w07Y4iToWJInouaZqwSJvUpD1sf70t2zx48JSv531_hAehKwIzApTMpYoz6WSt1rqZLUqgIPIjNCEphUTkOT1GEwBgCc0AztB5jF8AhAARp-iMkZzDImcT5JeyDFbJznqHvcFvttVBbq3TeNk7NV7L2m51hd98vWn0wOIX73xtu_Ww_bAtNj7gd9naCj-44XndaNeNVY_1RvlWt52tdJyPJ-niBToxso76cr9O0efy4eP-KVm9Pj7f360SyTLRJdmClKXggqeUl7JSVKiy4iZXTEklJANqjOFcq0oZASplROepUUxk1DDBMjZFt7veNvjvXseuaGxUuq6l076PBWU0YxldpCN68wf98n0Yvj1SLBWCszQfKL6jVPAxBm2KNthGhk1BoBiFFIOQ4iCk2AsZYtf78r5sdPUbOhgYANgBY_x38L-dPzb5nA4</recordid><startdate>20200107</startdate><enddate>20200107</enddate><creator>Sajid, Muhammad Salman</creator><creator>Jovcevski, Blagojce</creator><creator>Pukala, Tara Louise</creator><creator>Jabeen, Fahmida</creator><creator>Najam-ul-Haq, Muhammad</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-2090-5072</orcidid><orcidid>https://orcid.org/0000-0001-7999-1385</orcidid><orcidid>https://orcid.org/0000-0001-7391-1436</orcidid></search><sort><creationdate>20200107</creationdate><title>Fabrication of Piperazine Functionalized Polymeric Monolithic Tip for Rapid Enrichment of Glycopeptides/Glycans</title><author>Sajid, Muhammad Salman ; Jovcevski, Blagojce ; Pukala, Tara Louise ; Jabeen, Fahmida ; Najam-ul-Haq, Muhammad</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a376t-791bb6464524badc26cbd4f8c3cac6a302fff44ecdcf60c531e85fc3672f36373</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Animals</topic><topic>Antibiotics</topic><topic>Avidin</topic><topic>Biological properties</topic><topic>Biological samples</topic><topic>Bovine serum albumin</topic><topic>Cattle</topic><topic>Chemistry</topic><topic>Cost analysis</topic><topic>Elution</topic><topic>Enrichment</topic><topic>Fabrication</topic><topic>Free radical polymerization</topic><topic>Free radicals</topic><topic>Glycopeptides</topic><topic>Glycopeptides - blood</topic><topic>Glycopeptides - metabolism</topic><topic>Glycosylation</topic><topic>Horseradish Peroxidase - chemistry</topic><topic>Horseradish Peroxidase - metabolism</topic><topic>Horses</topic><topic>Humans</topic><topic>IgG antibody</topic><topic>Immunoglobulin G</topic><topic>Immunoglobulins</topic><topic>Ovalbumin</topic><topic>Peroxidase</topic><topic>Piperazine</topic><topic>Polymerization</topic><topic>Polymers - chemistry</topic><topic>Polysaccharides</topic><topic>Polysaccharides - blood</topic><topic>Polysaccharides - metabolism</topic><topic>Poultry</topic><topic>Pretreatment</topic><topic>Radishes</topic><topic>Selectivity</topic><topic>Separation</topic><topic>Serum albumin</topic><topic>Serum Albumin, Bovine - chemistry</topic><topic>Serum Albumin, Bovine - metabolism</topic><topic>Spectrometry</topic><topic>Tips</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sajid, Muhammad Salman</creatorcontrib><creatorcontrib>Jovcevski, Blagojce</creatorcontrib><creatorcontrib>Pukala, Tara Louise</creatorcontrib><creatorcontrib>Jabeen, Fahmida</creatorcontrib><creatorcontrib>Najam-ul-Haq, Muhammad</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sajid, Muhammad Salman</au><au>Jovcevski, Blagojce</au><au>Pukala, Tara Louise</au><au>Jabeen, Fahmida</au><au>Najam-ul-Haq, Muhammad</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fabrication of Piperazine Functionalized Polymeric Monolithic Tip for Rapid Enrichment of Glycopeptides/Glycans</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. Chem</addtitle><date>2020-01-07</date><risdate>2020</risdate><volume>92</volume><issue>1</issue><spage>683</spage><epage>689</epage><pages>683-689</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><abstract>Enrichment strategies are designed for the pretreatment of low-abundance glycans and glycopeptides prior to mass spectrometric (MS) analysis. Here, a tip-based strategy is being reported for the enrichment of glycopeptides and glycans using a piperazine modified polymeric monolithic tip. The tip is fabricated using the free radical polymerization. Fast separation (2 min) is achieved under optimized conditions with 20 cycles per step of loading, incubation, washing, and elution followed by MALDI-MS analysis. A total of 25, 22, and 34 glycopeptides covering all glycosylation sites are enriched by the modified tips from tryptic digests of horse radish peroxidase, chicken avidin, and human immunoglobulin G, respectively. Piperazine exhibits high selectivity 1:400 horse radish peroxidase/bovine serum albumin, sensitivity to 100 attomoles, recovery 89.51%, and batch to batch reproducibility (RSD > 1) in glycopeptides enrichment. Piperazine tips also enrich glycans from ovalbumin and human immunoglobulin G. High selectivity (1:1200, ovalbumin/BSA) and detection limit of 100 attomole is attained for glycans and furthermore 58 glycans are enriched from human serum. Thus, piperazine tips can be used as an enrichment tool for swift, cost-effective routine analysis of biological samples for separation of glycopeptides and glycans.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>31840983</pmid><doi>10.1021/acs.analchem.9b02068</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0002-2090-5072</orcidid><orcidid>https://orcid.org/0000-0001-7999-1385</orcidid><orcidid>https://orcid.org/0000-0001-7391-1436</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0003-2700 |
| ispartof | Analytical chemistry (Washington), 2020-01, Vol.92 (1), p.683-689 |
| issn | 0003-2700 1520-6882 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2327372957 |
| source | MEDLINE; ACS Publications |
| subjects | Animals Antibiotics Avidin Biological properties Biological samples Bovine serum albumin Cattle Chemistry Cost analysis Elution Enrichment Fabrication Free radical polymerization Free radicals Glycopeptides Glycopeptides - blood Glycopeptides - metabolism Glycosylation Horseradish Peroxidase - chemistry Horseradish Peroxidase - metabolism Horses Humans IgG antibody Immunoglobulin G Immunoglobulins Ovalbumin Peroxidase Piperazine Polymerization Polymers - chemistry Polysaccharides Polysaccharides - blood Polysaccharides - metabolism Poultry Pretreatment Radishes Selectivity Separation Serum albumin Serum Albumin, Bovine - chemistry Serum Albumin, Bovine - metabolism Spectrometry Tips |
| title | Fabrication of Piperazine Functionalized Polymeric Monolithic Tip for Rapid Enrichment of Glycopeptides/Glycans |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-18T07%3A46%3A12IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Fabrication%20of%20Piperazine%20Functionalized%20Polymeric%20Monolithic%20Tip%20for%20Rapid%20Enrichment%20of%20Glycopeptides/Glycans&rft.jtitle=Analytical%20chemistry%20(Washington)&rft.au=Sajid,%20Muhammad%20Salman&rft.date=2020-01-07&rft.volume=92&rft.issue=1&rft.spage=683&rft.epage=689&rft.pages=683-689&rft.issn=0003-2700&rft.eissn=1520-6882&rft_id=info:doi/10.1021/acs.analchem.9b02068&rft_dat=%3Cproquest_cross%3E2327372957%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2335664358&rft_id=info:pmid/31840983&rfr_iscdi=true |